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The map label for this gene is lcfB [H]

Identifier: 87201173

GI number: 87201173

Start: 3373450

End: 3375000

Strand: Direct

Name: lcfB [H]

Synonym: Saro_3161

Alternate gene names: 87201173

Gene position: 3373450-3375000 (Clockwise)

Preceding gene: 87201172

Following gene: 87201174

Centisome position: 94.72

GC content: 66.15

Gene sequence:

>1551_bases
GTGCACTTTCACCCCAAGTACCATGCCACCGCAAATCCCGATCGCGCAGCCGTGATCATGGGCGGCAGCGGCGAGGTGAT
GACCTATGCCGAGCTGGAGGCCCGATCGAACCGCTTCGCGCAGCTCATGCGCGCGCGCGGATTGCAGATCGGCGATACCA
TCGCGCTGTGCCTTGAGAACCGTGCCGACTACTTTCCCATCGCCTGGGGCGCGCAGCGCGCCGGGCTGGTCTATGTCGCC
ATATCCAGCCGCCTTGCCGCGCCCGAGATCGCCTACATCGCCAAGGACAGCGGAAGCCGGTTGCTGATCGGCTCTGCCTA
TACGGCGCCGGTACTCGACGAAGTGGCGAAGCTGGAACCGGGCGTGCCGCAACTGCGGCTCGATACCGATGGCCCGCTTT
CACTCGACGCGGCGATGGCGGAAATGCCGGATACCCCCATCGACGACGAACGCGCCGGGTGCGACATGCTCTATTCGTCG
GGCACGACCGGCAAGCCCAAGGGCGTGCGCATCCCCCTGCCCGAAGACCCGGAGATCGGCGCGGCCAATTCCCTCATGGC
CATCGCCAACCAGGCCTTCGGCATCGGCGGCGATGCGGTCTACCTCTCGCCCGCGCCGCTCTATCATGCGGCGCCGCTGC
GCTGGTCGATGACCATCCACCGCTTGGGCGGCACGGTGGTGGCAATGGAGAAGTTCGATCCCGAACACGCGCTCGAACTG
ATCGAGAAGCACAAGGTGACCGACAGCCAATGGGTGCCGACGCATTTCGTGCGGATGCTCAAGCTGCCGGACGAGGTGCG
GTCGAAGTACGATACCTCCTCGCTCAAGCTCGCGATCCATGCCGCCGCGCCCTGCCCGGTGCCGGTAAAGCAGGCGATGA
TCGAATGGTGGGGGCCGGCGCTGCGCGAATACTACGCCGGGACCGAAGGCAACGGCTTCACCTTCATTTCCAGCGAGGAA
TGGCTGAAGCGGCCCGGATCGGTCGGCCGCGCGCTGCTTGGCACCATCCGCATCTGCGACGAGAACGGCGATGAGGTGCC
GCCGCGCACCGAAGGGCAGGTCTTCTTCGAGGGCGGCAGCCCGTTCAGCTACCACAACGATCCCGACAAGACCCGCGACG
CCACCAACAAGCATGGCTGGACCAGCCTTGGCGACGTGGGCTGGGTGGACGAGGACGGCTACCTGTTCCTCACCGACCGC
AAGAGCTTCATGATCATTTCGGGTGGCGTGAACATCTATCCGCAGGAGATCGAGAACCTGCTGGTCACGCATCCCAAGGT
GGCTGACGTGGCGGTGATCGGCGCGCCCGATCCGGACATGGGCGAACGCGTGGTGGCGGTGGTCCAGCCGCGCGACATGG
CCGAGGCCGGGCCCGCGCTGGCGGCGGAACTGACCGAGTGGCTCGCCCCCCAGCTTTCGCGCGTGAAGATGCCGCGCCAG
ATCGATTTCCGCGCCGAACTCCCGCGCGAACCGACCGGCAAGCTGTTCAAGCGCCTGCTGCGTGACGAATACAAGCAGGC
TTACGAAGCGGCGCAGGCCGCGGCGGTCTGA

Upstream 100 bases:

>100_bases
CCGGCGCGGCGCGAATCCTGGTTGCGCCGCTTAAGTGATCGGTTAAACTCTCGCGGAAAAGGTGGCTTGAAGCCGCCAGG
AACGAGAGAGGACGAGGCCG

Downstream 100 bases:

>100_bases
CGGCAAGCGGGAGGGTTTTATGGACAGCATTCCGATGGTGCCGGCCGAAGTCGGCCGCGCGGTGATAGACCCGAAGTCCT
ACGGGACGTGGGAGCCCTTG

Product: long-chain-fatty-acid--CoA ligase

Products: NA

Alternate protein names: Long-chain acyl-CoA synthetase [H]

Number of amino acids: Translated: 516; Mature: 516

Protein sequence:

>516_residues
MHFHPKYHATANPDRAAVIMGGSGEVMTYAELEARSNRFAQLMRARGLQIGDTIALCLENRADYFPIAWGAQRAGLVYVA
ISSRLAAPEIAYIAKDSGSRLLIGSAYTAPVLDEVAKLEPGVPQLRLDTDGPLSLDAAMAEMPDTPIDDERAGCDMLYSS
GTTGKPKGVRIPLPEDPEIGAANSLMAIANQAFGIGGDAVYLSPAPLYHAAPLRWSMTIHRLGGTVVAMEKFDPEHALEL
IEKHKVTDSQWVPTHFVRMLKLPDEVRSKYDTSSLKLAIHAAAPCPVPVKQAMIEWWGPALREYYAGTEGNGFTFISSEE
WLKRPGSVGRALLGTIRICDENGDEVPPRTEGQVFFEGGSPFSYHNDPDKTRDATNKHGWTSLGDVGWVDEDGYLFLTDR
KSFMIISGGVNIYPQEIENLLVTHPKVADVAVIGAPDPDMGERVVAVVQPRDMAEAGPALAAELTEWLAPQLSRVKMPRQ
IDFRAELPREPTGKLFKRLLRDEYKQAYEAAQAAAV

Sequences:

>Translated_516_residues
MHFHPKYHATANPDRAAVIMGGSGEVMTYAELEARSNRFAQLMRARGLQIGDTIALCLENRADYFPIAWGAQRAGLVYVA
ISSRLAAPEIAYIAKDSGSRLLIGSAYTAPVLDEVAKLEPGVPQLRLDTDGPLSLDAAMAEMPDTPIDDERAGCDMLYSS
GTTGKPKGVRIPLPEDPEIGAANSLMAIANQAFGIGGDAVYLSPAPLYHAAPLRWSMTIHRLGGTVVAMEKFDPEHALEL
IEKHKVTDSQWVPTHFVRMLKLPDEVRSKYDTSSLKLAIHAAAPCPVPVKQAMIEWWGPALREYYAGTEGNGFTFISSEE
WLKRPGSVGRALLGTIRICDENGDEVPPRTEGQVFFEGGSPFSYHNDPDKTRDATNKHGWTSLGDVGWVDEDGYLFLTDR
KSFMIISGGVNIYPQEIENLLVTHPKVADVAVIGAPDPDMGERVVAVVQPRDMAEAGPALAAELTEWLAPQLSRVKMPRQ
IDFRAELPREPTGKLFKRLLRDEYKQAYEAAQAAAV
>Mature_516_residues
MHFHPKYHATANPDRAAVIMGGSGEVMTYAELEARSNRFAQLMRARGLQIGDTIALCLENRADYFPIAWGAQRAGLVYVA
ISSRLAAPEIAYIAKDSGSRLLIGSAYTAPVLDEVAKLEPGVPQLRLDTDGPLSLDAAMAEMPDTPIDDERAGCDMLYSS
GTTGKPKGVRIPLPEDPEIGAANSLMAIANQAFGIGGDAVYLSPAPLYHAAPLRWSMTIHRLGGTVVAMEKFDPEHALEL
IEKHKVTDSQWVPTHFVRMLKLPDEVRSKYDTSSLKLAIHAAAPCPVPVKQAMIEWWGPALREYYAGTEGNGFTFISSEE
WLKRPGSVGRALLGTIRICDENGDEVPPRTEGQVFFEGGSPFSYHNDPDKTRDATNKHGWTSLGDVGWVDEDGYLFLTDR
KSFMIISGGVNIYPQEIENLLVTHPKVADVAVIGAPDPDMGERVVAVVQPRDMAEAGPALAAELTEWLAPQLSRVKMPRQ
IDFRAELPREPTGKLFKRLLRDEYKQAYEAAQAAAV

Specific function: Involved in the degradation of long-chain fatty acids [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=544, Percent_Identity=27.3897058823529, Blast_Score=154, Evalue=1e-37,
Organism=Homo sapiens, GI187761345, Length=518, Percent_Identity=29.1505791505791, Blast_Score=151, Evalue=1e-36,
Organism=Homo sapiens, GI187761343, Length=518, Percent_Identity=29.1505791505791, Blast_Score=151, Evalue=1e-36,
Organism=Homo sapiens, GI157311624, Length=532, Percent_Identity=27.4436090225564, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI157311622, Length=532, Percent_Identity=27.4436090225564, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI42544132, Length=526, Percent_Identity=26.425855513308, Blast_Score=132, Evalue=7e-31,
Organism=Homo sapiens, GI58082049, Length=528, Percent_Identity=27.2727272727273, Blast_Score=132, Evalue=9e-31,
Organism=Homo sapiens, GI122937307, Length=518, Percent_Identity=27.027027027027, Blast_Score=123, Evalue=5e-28,
Organism=Homo sapiens, GI115511026, Length=520, Percent_Identity=25.1923076923077, Blast_Score=122, Evalue=8e-28,
Organism=Homo sapiens, GI38505220, Length=523, Percent_Identity=25.8126195028681, Blast_Score=117, Evalue=3e-26,
Organism=Homo sapiens, GI28416953, Length=542, Percent_Identity=24.169741697417, Blast_Score=93, Evalue=5e-19,
Organism=Homo sapiens, GI38524616, Length=547, Percent_Identity=25.9597806215722, Blast_Score=92, Evalue=9e-19,
Organism=Homo sapiens, GI62865631, Length=526, Percent_Identity=23.384030418251, Blast_Score=85, Evalue=2e-16,
Organism=Homo sapiens, GI13325055, Length=526, Percent_Identity=23.384030418251, Blast_Score=85, Evalue=2e-16,
Organism=Homo sapiens, GI8923896, Length=580, Percent_Identity=21.551724137931, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI253970489, Length=437, Percent_Identity=24.0274599542334, Blast_Score=69, Evalue=2e-11,
Organism=Escherichia coli, GI1788107, Length=527, Percent_Identity=29.0322580645161, Blast_Score=160, Evalue=3e-40,
Organism=Escherichia coli, GI221142682, Length=517, Percent_Identity=26.3056092843327, Blast_Score=146, Evalue=2e-36,
Organism=Escherichia coli, GI145693145, Length=521, Percent_Identity=26.8714011516315, Blast_Score=145, Evalue=5e-36,
Organism=Escherichia coli, GI1790505, Length=559, Percent_Identity=24.5080500894454, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI1786810, Length=510, Percent_Identity=24.7058823529412, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI1786529, Length=526, Percent_Identity=22.4334600760456, Blast_Score=74, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17531443, Length=527, Percent_Identity=29.4117647058824, Blast_Score=186, Evalue=3e-47,
Organism=Caenorhabditis elegans, GI17558820, Length=516, Percent_Identity=28.2945736434109, Blast_Score=183, Evalue=2e-46,
Organism=Caenorhabditis elegans, GI32563687, Length=488, Percent_Identity=27.2540983606557, Blast_Score=163, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI71994690, Length=546, Percent_Identity=27.1062271062271, Blast_Score=155, Evalue=4e-38,
Organism=Caenorhabditis elegans, GI71994694, Length=546, Percent_Identity=27.1062271062271, Blast_Score=155, Evalue=4e-38,
Organism=Caenorhabditis elegans, GI71994703, Length=546, Percent_Identity=27.1062271062271, Blast_Score=155, Evalue=4e-38,
Organism=Caenorhabditis elegans, GI17560308, Length=535, Percent_Identity=25.7943925233645, Blast_Score=135, Evalue=6e-32,
Organism=Caenorhabditis elegans, GI17557194, Length=523, Percent_Identity=26.7686424474187, Blast_Score=125, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI17559526, Length=379, Percent_Identity=29.023746701847, Blast_Score=124, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17538037, Length=520, Percent_Identity=25.5769230769231, Blast_Score=119, Evalue=5e-27,
Organism=Caenorhabditis elegans, GI17560140, Length=506, Percent_Identity=24.1106719367589, Blast_Score=110, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI17551278, Length=561, Percent_Identity=25.8467023172906, Blast_Score=107, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI115534440, Length=521, Percent_Identity=24.3761996161228, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI71985884, Length=246, Percent_Identity=29.6747967479675, Blast_Score=77, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6319699, Length=361, Percent_Identity=27.4238227146814, Blast_Score=118, Evalue=3e-27,
Organism=Saccharomyces cerevisiae, GI6323182, Length=575, Percent_Identity=24.3478260869565, Blast_Score=92, Evalue=3e-19,
Organism=Drosophila melanogaster, GI21355181, Length=513, Percent_Identity=26.5107212475634, Blast_Score=143, Evalue=3e-34,
Organism=Drosophila melanogaster, GI24581924, Length=545, Percent_Identity=24.0366972477064, Blast_Score=132, Evalue=5e-31,
Organism=Drosophila melanogaster, GI21356441, Length=504, Percent_Identity=24.6031746031746, Blast_Score=129, Evalue=5e-30,
Organism=Drosophila melanogaster, GI24648253, Length=456, Percent_Identity=26.3157894736842, Blast_Score=126, Evalue=3e-29,
Organism=Drosophila melanogaster, GI24648255, Length=456, Percent_Identity=26.5350877192982, Blast_Score=126, Evalue=4e-29,
Organism=Drosophila melanogaster, GI18859661, Length=529, Percent_Identity=26.2759924385633, Blast_Score=125, Evalue=7e-29,
Organism=Drosophila melanogaster, GI24648257, Length=511, Percent_Identity=24.853228962818, Blast_Score=119, Evalue=4e-27,
Organism=Drosophila melanogaster, GI24648260, Length=511, Percent_Identity=24.26614481409, Blast_Score=119, Evalue=5e-27,
Organism=Drosophila melanogaster, GI21356947, Length=506, Percent_Identity=23.7154150197628, Blast_Score=118, Evalue=1e-26,
Organism=Drosophila melanogaster, GI161076582, Length=529, Percent_Identity=23.8185255198488, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI21358303, Length=443, Percent_Identity=25.2821670428894, Blast_Score=117, Evalue=3e-26,
Organism=Drosophila melanogaster, GI281365686, Length=465, Percent_Identity=26.4516129032258, Blast_Score=116, Evalue=3e-26,
Organism=Drosophila melanogaster, GI24653035, Length=525, Percent_Identity=24.1904761904762, Blast_Score=116, Evalue=4e-26,
Organism=Drosophila melanogaster, GI20130357, Length=514, Percent_Identity=23.3463035019455, Blast_Score=102, Evalue=7e-22,
Organism=Drosophila melanogaster, GI24656500, Length=521, Percent_Identity=23.4165067178503, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI62472339, Length=535, Percent_Identity=23.7383177570093, Blast_Score=97, Evalue=4e-20,
Organism=Drosophila melanogaster, GI24667955, Length=535, Percent_Identity=23.7383177570093, Blast_Score=96, Evalue=6e-20,
Organism=Drosophila melanogaster, GI281364798, Length=520, Percent_Identity=23.8461538461538, Blast_Score=92, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24583465, Length=520, Percent_Identity=23.8461538461538, Blast_Score=92, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24583463, Length=520, Percent_Identity=23.8461538461538, Blast_Score=92, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24667959, Length=368, Percent_Identity=25.8152173913043, Blast_Score=87, Evalue=4e-17,
Organism=Drosophila melanogaster, GI21355225, Length=374, Percent_Identity=24.331550802139, Blast_Score=74, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020459
- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.3 [H]

Molecular weight: Translated: 56449; Mature: 56449

Theoretical pI: Translated: 5.08; Mature: 5.08

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHFHPKYHATANPDRAAVIMGGSGEVMTYAELEARSNRFAQLMRARGLQIGDTIALCLEN
CCCCCCCCCCCCCCCEEEEECCCCCEEEEEHHHHHHHHHHHHHHHCCCCCCCHHEEEECC
RADYFPIAWGAQRAGLVYVAISSRLAAPEIAYIAKDSGSRLLIGSAYTAPVLDEVAKLEP
CCCEEEEECCCCCCCEEEEEECCCCCCCEEEEEEECCCCEEEEECCCCHHHHHHHHHCCC
GVPQLRLDTDGPLSLDAAMAEMPDTPIDDERAGCDMLYSSGTTGKPKGVRIPLPEDPEIG
CCCEEEECCCCCCCHHHHHHHCCCCCCCCCCCCCHHEECCCCCCCCCCEECCCCCCCCCC
AANSLMAIANQAFGIGGDAVYLSPAPLYHAAPLRWSMTIHRLGGTVVAMEKFDPEHALEL
CCHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCEEEEEEEECCCEEEEEECCCHHHHHHH
IEKHKVTDSQWVPTHFVRMLKLPDEVRSKYDTSSLKLAIHAAAPCPVPVKQAMIEWWGPA
HHHHCCCCCCCCHHHHHHHHCCCHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHCHH
LREYYAGTEGNGFTFISSEEWLKRPGSVGRALLGTIRICDENGDEVPPRTEGQVFFEGGS
HHHHHCCCCCCCEEEECCHHHHHCCCHHHHHHHHEEEEECCCCCCCCCCCCCEEEEECCC
PFSYHNDPDKTRDATNKHGWTSLGDVGWVDEDGYLFLTDRKSFMIISGGVNIYPQEIENL
CCCCCCCCCHHHCCCCCCCCCCCCCCCEECCCCEEEEECCCEEEEEECCCEECHHHHHHH
LVTHPKVADVAVIGAPDPDMGERVVAVVQPRDMAEAGPALAAELTEWLAPQLSRVKMPRQ
HHCCCCCCCEEEEECCCCCCCCEEEEEECCCCHHHCCCHHHHHHHHHHHHHHHHCCCCCC
IDFRAELPREPTGKLFKRLLRDEYKQAYEAAQAAAV
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MHFHPKYHATANPDRAAVIMGGSGEVMTYAELEARSNRFAQLMRARGLQIGDTIALCLEN
CCCCCCCCCCCCCCCEEEEECCCCCEEEEEHHHHHHHHHHHHHHHCCCCCCCHHEEEECC
RADYFPIAWGAQRAGLVYVAISSRLAAPEIAYIAKDSGSRLLIGSAYTAPVLDEVAKLEP
CCCEEEEECCCCCCCEEEEEECCCCCCCEEEEEEECCCCEEEEECCCCHHHHHHHHHCCC
GVPQLRLDTDGPLSLDAAMAEMPDTPIDDERAGCDMLYSSGTTGKPKGVRIPLPEDPEIG
CCCEEEECCCCCCCHHHHHHHCCCCCCCCCCCCCHHEECCCCCCCCCCEECCCCCCCCCC
AANSLMAIANQAFGIGGDAVYLSPAPLYHAAPLRWSMTIHRLGGTVVAMEKFDPEHALEL
CCHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCEEEEEEEECCCEEEEEECCCHHHHHHH
IEKHKVTDSQWVPTHFVRMLKLPDEVRSKYDTSSLKLAIHAAAPCPVPVKQAMIEWWGPA
HHHHCCCCCCCCHHHHHHHHCCCHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHCHH
LREYYAGTEGNGFTFISSEEWLKRPGSVGRALLGTIRICDENGDEVPPRTEGQVFFEGGS
HHHHHCCCCCCCEEEECCHHHHHCCCHHHHHHHHEEEEECCCCCCCCCCCCCEEEEECCC
PFSYHNDPDKTRDATNKHGWTSLGDVGWVDEDGYLFLTDRKSFMIISGGVNIYPQEIENL
CCCCCCCCCHHHCCCCCCCCCCCCCCCEECCCCEEEEECCCEEEEEECCCEECHHHHHHH
LVTHPKVADVAVIGAPDPDMGERVVAVVQPRDMAEAGPALAAELTEWLAPQLSRVKMPRQ
HHCCCCCCCEEEEECCCCCCCCEEEEEECCCCHHHCCCHHHHHHHHHHHHHHHHCCCCCC
IDFRAELPREPTGKLFKRLLRDEYKQAYEAAQAAAV
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377 [H]