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The map label for this gene is rfbB [C]

Identifier: 87201163

GI number: 87201163

Start: 3363104

End: 3364102

Strand: Direct

Name: rfbB [C]

Synonym: Saro_3151

Alternate gene names: 87201163

Gene position: 3363104-3364102 (Clockwise)

Preceding gene: 87201162

Following gene: 87201166

Centisome position: 94.43

GC content: 65.97

Gene sequence:

>999_bases
GTGAAAGTCCTCGTAACCGGAGCCGCCGGCTTCATCGGCTACTCGCTCGCCACCCGCCTTCTCGCACGGGGCGACGAGGT
GATCGGCGTCGATATCGTCAACGACTACTACGATCCGCGCCTCAAGGAGGCCCGCCTCGCCCGTCTCGCCCAGCAGGGCG
GCGGCCGCTTCACCTTCCTGCGCACCGACTTCGCCGATTACCCCGCGCTGACCGCAGCGCTCGAAGGCGCGCACTTCGAC
CGCATCGTCCACCTCGGCGCGCAGGCGGGCGTGCGCTATTCGATCGAGAACCCCCACGCCTACGTGCAGTCGAACCTCGT
CGGCCACGTCAACCTGCTCGAGGTTGCGCGCCATCGCGGGGTCGAACACATGGTCTACGCGTCCTCGTCCTCGGTCTACG
GCGGCAACACCAAGCTGCCCTTTTCGGTCGACGACCGCGTCGACCATCCGCTCTCGCTCTACGCCGCCACCAAGAAGGCG
GACGAGTTGATGAGCGAGACTTACGCCCACCTCTATCGCTTGCCGCTCACCGGCCTGCGCTTCTTCACCGTCTATGGCCC
GTGGGGCCGCCCGGACATGATGATGTGGCTGTTCACCCGCGCGATCCTCGCGGGCGAACCGATCCAGGTCTTCAACCACG
GCGACATGTACCGCGACTTCACCTATGTCGACGACATCGTCTCCGGCGTGGTCGCCTGCCTCGACAACCCGCCGCTCGAC
GACGGCGCGCCCAAGGCCGGCGGCAGCCTCAAGCCGCACCGGCTCTACAACATCGGCAACCACAAGTCGGAACACCTGAT
GAAGGTGATCGCCATTCTCGAAGCCGAGCTTGGCCGCAAGGCGGAGATGCGGATGCTGCCGATGCAGCCCGGCGACGTGC
GCCAGTCCTTCGCCGATATCGACGCGATCTCCGGCGATCTAGGCTACCGGCCGACGACCGGCATCGAAACGGGCGTTCCG
AACTTCGTCCGCTGGTACAAGGACTACCACGGCCTCTGA

Upstream 100 bases:

>100_bases
CCCGGTCATCTGGCCCGGTCGTCTGGCCCGGACGGGCAGGCAATGACGTGCTAGGCCTGAAACGCGAATCGAAGACCTGC
GAATTTCCGGAGAAAGCTGC

Downstream 100 bases:

>100_bases
CGCCGGGCAGTCCTCGCCCGTTTTCCGGTCAGGCCGCCGAGGCCCGCGATGCGCCGGCCCTTGGCATCAGTGCGCCCAGC
ACGACGGCAACCAGCACCAG

Product: NAD-dependent epimerase/dehydratase

Products: dTDP-4-dehydro-6-deoxy-D-glucose; H2O [C]

Alternate protein names: ORF2 [H]

Number of amino acids: Translated: 332; Mature: 332

Protein sequence:

>332_residues
MKVLVTGAAGFIGYSLATRLLARGDEVIGVDIVNDYYDPRLKEARLARLAQQGGGRFTFLRTDFADYPALTAALEGAHFD
RIVHLGAQAGVRYSIENPHAYVQSNLVGHVNLLEVARHRGVEHMVYASSSSVYGGNTKLPFSVDDRVDHPLSLYAATKKA
DELMSETYAHLYRLPLTGLRFFTVYGPWGRPDMMMWLFTRAILAGEPIQVFNHGDMYRDFTYVDDIVSGVVACLDNPPLD
DGAPKAGGSLKPHRLYNIGNHKSEHLMKVIAILEAELGRKAEMRMLPMQPGDVRQSFADIDAISGDLGYRPTTGIETGVP
NFVRWYKDYHGL

Sequences:

>Translated_332_residues
MKVLVTGAAGFIGYSLATRLLARGDEVIGVDIVNDYYDPRLKEARLARLAQQGGGRFTFLRTDFADYPALTAALEGAHFD
RIVHLGAQAGVRYSIENPHAYVQSNLVGHVNLLEVARHRGVEHMVYASSSSVYGGNTKLPFSVDDRVDHPLSLYAATKKA
DELMSETYAHLYRLPLTGLRFFTVYGPWGRPDMMMWLFTRAILAGEPIQVFNHGDMYRDFTYVDDIVSGVVACLDNPPLD
DGAPKAGGSLKPHRLYNIGNHKSEHLMKVIAILEAELGRKAEMRMLPMQPGDVRQSFADIDAISGDLGYRPTTGIETGVP
NFVRWYKDYHGL
>Mature_332_residues
MKVLVTGAAGFIGYSLATRLLARGDEVIGVDIVNDYYDPRLKEARLARLAQQGGGRFTFLRTDFADYPALTAALEGAHFD
RIVHLGAQAGVRYSIENPHAYVQSNLVGHVNLLEVARHRGVEHMVYASSSSVYGGNTKLPFSVDDRVDHPLSLYAATKKA
DELMSETYAHLYRLPLTGLRFFTVYGPWGRPDMMMWLFTRAILAGEPIQVFNHGDMYRDFTYVDDIVSGVVACLDNPPLD
DGAPKAGGSLKPHRLYNIGNHKSEHLMKVIAILEAELGRKAEMRMLPMQPGDVRQSFADIDAISGDLGYRPTTGIETGVP
NFVRWYKDYHGL

Specific function: DTDP-L-RHAMNOSE BIOSYNTHESIS WITHIN THE O ANTIGEN BIOSYNTHESIS PATHWAY OF LIPOPOLYSACCHARIDE BIOSYNTHESIS. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI56237023, Length=347, Percent_Identity=25.3602305475504, Blast_Score=102, Evalue=5e-22,
Organism=Homo sapiens, GI56118217, Length=347, Percent_Identity=25.3602305475504, Blast_Score=102, Evalue=5e-22,
Organism=Homo sapiens, GI189083684, Length=347, Percent_Identity=25.3602305475504, Blast_Score=102, Evalue=5e-22,
Organism=Homo sapiens, GI7657641, Length=334, Percent_Identity=25.1497005988024, Blast_Score=99, Evalue=5e-21,
Organism=Homo sapiens, GI42516563, Length=336, Percent_Identity=24.702380952381, Blast_Score=95, Evalue=7e-20,
Organism=Escherichia coli, GI1788353, Length=353, Percent_Identity=26.9121813031161, Blast_Score=118, Evalue=6e-28,
Organism=Escherichia coli, GI48994969, Length=348, Percent_Identity=27.2988505747126, Blast_Score=104, Evalue=9e-24,
Organism=Escherichia coli, GI1786974, Length=343, Percent_Identity=22.7405247813411, Blast_Score=98, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI71982035, Length=346, Percent_Identity=26.878612716763, Blast_Score=115, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI71982038, Length=348, Percent_Identity=26.4367816091954, Blast_Score=112, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI17568069, Length=342, Percent_Identity=25.4385964912281, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI115532424, Length=333, Percent_Identity=24.024024024024, Blast_Score=88, Evalue=8e-18,
Organism=Caenorhabditis elegans, GI17539532, Length=337, Percent_Identity=25.5192878338279, Blast_Score=80, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6319493, Length=348, Percent_Identity=25.2873563218391, Blast_Score=82, Evalue=8e-17,
Organism=Drosophila melanogaster, GI19923002, Length=343, Percent_Identity=26.530612244898, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI21356223, Length=337, Percent_Identity=24.9258160237389, Blast_Score=91, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040
- InterPro:   IPR008089 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 4.2.1.46 [C]

Molecular weight: Translated: 36879; Mature: 36879

Theoretical pI: Translated: 6.85; Mature: 6.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVLVTGAAGFIGYSLATRLLARGDEVIGVDIVNDYYDPRLKEARLARLAQQGGGRFTFL
CEEEEECCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCEEEEE
RTDFADYPALTAALEGAHFDRIVHLGAQAGVRYSIENPHAYVQSNLVGHVNLLEVARHRG
ECCCCCCHHHHHHHCCCHHHHHHHHCCCCCEEEEECCCHHHHHHCCCCCHHHHHHHHHCC
VEHMVYASSSSVYGGNTKLPFSVDDRVDHPLSLYAATKKADELMSETYAHLYRLPLTGLR
CEEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEE
FFTVYGPWGRPDMMMWLFTRAILAGEPIQVFNHGDMYRDFTYVDDIVSGVVACLDNPPLD
EEEEECCCCCCHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCC
DGAPKAGGSLKPHRLYNIGNHKSEHLMKVIAILEAELGRKAEMRMLPMQPGDVRQSFADI
CCCCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHCCCCCEEECCCCCCHHHHHHHHH
DAISGDLGYRPTTGIETGVPNFVRWYKDYHGL
HHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MKVLVTGAAGFIGYSLATRLLARGDEVIGVDIVNDYYDPRLKEARLARLAQQGGGRFTFL
CEEEEECCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCEEEEE
RTDFADYPALTAALEGAHFDRIVHLGAQAGVRYSIENPHAYVQSNLVGHVNLLEVARHRG
ECCCCCCHHHHHHHCCCHHHHHHHHCCCCCEEEEECCCHHHHHHCCCCCHHHHHHHHHCC
VEHMVYASSSSVYGGNTKLPFSVDDRVDHPLSLYAATKKADELMSETYAHLYRLPLTGLR
CEEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEE
FFTVYGPWGRPDMMMWLFTRAILAGEPIQVFNHGDMYRDFTYVDDIVSGVVACLDNPPLD
EEEEECCCCCCHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCC
DGAPKAGGSLKPHRLYNIGNHKSEHLMKVIAILEAELGRKAEMRMLPMQPGDVRQSFADI
CCCCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHCCCCCEEECCCCCCHHHHHHHHH
DAISGDLGYRPTTGIETGVPNFVRWYKDYHGL
HHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.019 {NAD+}} 0.034 {dTDPglucose}} [C]

Substrates: dTDPglucose [C]

Specific reaction: dTDPglucose --> dTDP-4-dehydro-6-deoxy-D-glucose + H2O [C]

General reaction: Elimination (of H2O C-O bond cleavage [C]

Inhibitor: TDP; TTP [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7682279 [H]