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The map label for this gene is epsD [H]

Identifier: 87201160

GI number: 87201160

Start: 3359153

End: 3360457

Strand: Reverse

Name: epsD [H]

Synonym: Saro_3148

Alternate gene names: 87201160

Gene position: 3360457-3359153 (Counterclockwise)

Preceding gene: 87201161

Following gene: 87201158

Centisome position: 94.35

GC content: 67.05

Gene sequence:

>1305_bases
ATGATGGGTGATGTGAAGCCGGACGTCTGTGTCGTCGGCCTTGGCTATATCGGACTTCCCACCGCCGCCATCATTGCCCG
CGCCGGCTGCCGCGTGCTGGGCCTCGACGTTTCGCAGAAGGTGGTCGACACGATCAACCGGGGCGAGATTCACATCGAGG
AAGTGGACCTCGACGGTCTTGTCCATGGCGTCGTCCAGCGCGGGCTGCTGCGCGCCTCGACCGAGATTGCGCCCGCCGAC
GTCTTCGTGATCGCGGTGCCGACGCCTTTCGAGAAGGACGGCCACCACACGCCCGACATTTCCTATGTGCTGGCCGCTGC
GACGCAGGTGGCCGCCGTGCTCAAGGCGGGCGACACGGTGATCCTCGAATCGACCTCGCCGGTCGGCACGACCGAACAGA
TGCGCGACCTGATGGCGGGGCTGCGGCCGGACCTGAAGTTTCCGGGCAAGACGCGCGAGACGCCCGACGTTTCGATCGCC
TATTGCCCCGAGCGCGTGCTGCCGGGGCGCATCCTGGAAGAGCTGACCAACAACGACCGTTCCATCGGCGGCATCACGCC
GCGCTGCGCACGCAAGGCACTGGCATTCTACAAGCGCTTCGTGCGCGGAACCTGCGTGACCACCGATGCCCGCTCTGCCG
AAATGACCAAGCTGGTCGAGAACGCCTATCGCGACGTCAACATCGCCTTCGCAAACGAGCTTTCGATGGTCGCGGACCGC
ATGGGGCTAGACGTGTGGGAGGTCATCCGTCTTGCCAACCGCCACCCGCGCGTGAACATCCTCCAGCCAGGGCCGGGCGT
GGGCGGGCATTGCATCGCGGTGGACCCGTGGTTCATCGTCGCCGGCGCACCCGACGAGACGCCGCTTATCCGCACCGCGC
GCAACGTCAACGACGGCAAGATGCATCACGTGGTGAAGAAGGCGACGGAACTGGTCGAAGCTCACCCCGGCGAAAAGGTC
GCGTGCCTCGGCCTCGCGTTCAAGGCGAACATCGACGACTTCCGCGAGAGCCCGGCGCGCTATGTCGCGGCGAGCCTAGC
CCGGCGCTTCGGCAGCCGCGTCCACATCGTCGAGCCCTATGCCGGCACCCTGCCGCGCGAATTCGAGGGCACCGGCGCGG
TGCAGATCGACGTCGACACGGCGCTGGAGGATTGCGGCGTGCTGATCGTGCTGGTCGACCACGACGTGTTCAAGGTGATC
CCGCTCGCCGAGCGCGCGGGCAAGGTGGTCTACGACACGCGCGGCATCTGGCCGGACCAGCCGGGCGCCGAAAGCGCTGG
CGCGGGTTTGCGGCTGGCGAGCTAG

Upstream 100 bases:

>100_bases
ACCCTGCTCGACGATAAGGCTGCCTATTCGGCCATGGCGCGCGCTCACAATCCCTTCGGGGATGGGCAATCTTCGCGCCG
AATCGTGGAGTTGCTGGCGA

Downstream 100 bases:

>100_bases
GCCGTAAACTCATAGAGGGCGCCATCGAGGTTTGAGGCAAAACGGCCAGGTGCAAGGAAGGAGGGCGCAGGAATATGTCG
ATATTTCAAGCCCGACTGAC

Product: UDP-glucose/GDP-mannose dehydrogenase

Products: UDP-N-acetyl-D-mannosaminuronic-acid; NADH; Proton [C]

Alternate protein names: NA

Number of amino acids: Translated: 434; Mature: 434

Protein sequence:

>434_residues
MMGDVKPDVCVVGLGYIGLPTAAIIARAGCRVLGLDVSQKVVDTINRGEIHIEEVDLDGLVHGVVQRGLLRASTEIAPAD
VFVIAVPTPFEKDGHHTPDISYVLAAATQVAAVLKAGDTVILESTSPVGTTEQMRDLMAGLRPDLKFPGKTRETPDVSIA
YCPERVLPGRILEELTNNDRSIGGITPRCARKALAFYKRFVRGTCVTTDARSAEMTKLVENAYRDVNIAFANELSMVADR
MGLDVWEVIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVAGAPDETPLIRTARNVNDGKMHHVVKKATELVEAHPGEKV
ACLGLAFKANIDDFRESPARYVAASLARRFGSRVHIVEPYAGTLPREFEGTGAVQIDVDTALEDCGVLIVLVDHDVFKVI
PLAERAGKVVYDTRGIWPDQPGAESAGAGLRLAS

Sequences:

>Translated_434_residues
MMGDVKPDVCVVGLGYIGLPTAAIIARAGCRVLGLDVSQKVVDTINRGEIHIEEVDLDGLVHGVVQRGLLRASTEIAPAD
VFVIAVPTPFEKDGHHTPDISYVLAAATQVAAVLKAGDTVILESTSPVGTTEQMRDLMAGLRPDLKFPGKTRETPDVSIA
YCPERVLPGRILEELTNNDRSIGGITPRCARKALAFYKRFVRGTCVTTDARSAEMTKLVENAYRDVNIAFANELSMVADR
MGLDVWEVIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVAGAPDETPLIRTARNVNDGKMHHVVKKATELVEAHPGEKV
ACLGLAFKANIDDFRESPARYVAASLARRFGSRVHIVEPYAGTLPREFEGTGAVQIDVDTALEDCGVLIVLVDHDVFKVI
PLAERAGKVVYDTRGIWPDQPGAESAGAGLRLAS
>Mature_434_residues
MMGDVKPDVCVVGLGYIGLPTAAIIARAGCRVLGLDVSQKVVDTINRGEIHIEEVDLDGLVHGVVQRGLLRASTEIAPAD
VFVIAVPTPFEKDGHHTPDISYVLAAATQVAAVLKAGDTVILESTSPVGTTEQMRDLMAGLRPDLKFPGKTRETPDVSIA
YCPERVLPGRILEELTNNDRSIGGITPRCARKALAFYKRFVRGTCVTTDARSAEMTKLVENAYRDVNIAFANELSMVADR
MGLDVWEVIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVAGAPDETPLIRTARNVNDGKMHHVVKKATELVEAHPGEKV
ACLGLAFKANIDDFRESPARYVAASLARRFGSRVHIVEPYAGTLPREFEGTGAVQIDVDTALEDCGVLIVLVDHDVFKVI
PLAERAGKVVYDTRGIWPDQPGAESAGAGLRLAS

Specific function: Probably involved in the synthesis of sugar components of EPS I, by converting NDP-N-acetyl-D-galactosamine into NDP-N- acetyl-D-galactosaminuronic acid [H]

COG id: COG0677

COG function: function code M; UDP-N-acetyl-D-mannosaminuronate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the UDP-glucose/GDP-mannose dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI4507813, Length=388, Percent_Identity=27.0618556701031, Blast_Score=112, Evalue=6e-25,
Organism=Homo sapiens, GI296040438, Length=283, Percent_Identity=26.8551236749117, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI296040443, Length=225, Percent_Identity=28.4444444444444, Blast_Score=74, Evalue=2e-13,
Organism=Escherichia coli, GI48994968, Length=424, Percent_Identity=54.0094339622642, Blast_Score=442, Evalue=1e-125,
Organism=Escherichia coli, GI1788340, Length=361, Percent_Identity=23.2686980609418, Blast_Score=64, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17560350, Length=368, Percent_Identity=29.3478260869565, Blast_Score=112, Evalue=5e-25,
Organism=Drosophila melanogaster, GI17136908, Length=374, Percent_Identity=28.0748663101604, Blast_Score=112, Evalue=6e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008927
- InterPro:   IPR016040
- InterPro:   IPR017476
- InterPro:   IPR014027
- InterPro:   IPR014026
- InterPro:   IPR014028
- InterPro:   IPR001732 [H]

Pfam domain/function: PF00984 UDPG_MGDP_dh; PF03720 UDPG_MGDP_dh_C; PF03721 UDPG_MGDP_dh_N [H]

EC number: 1.1.1.- [C]

Molecular weight: Translated: 46753; Mature: 46753

Theoretical pI: Translated: 6.14; Mature: 6.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMGDVKPDVCVVGLGYIGLPTAAIIARAGCRVLGLDVSQKVVDTINRGEIHIEEVDLDGL
CCCCCCCCEEEEECCHHHHHHHHHHHHCCCEEEECCHHHHHHHHCCCCEEEEEEECHHHH
VHGVVQRGLLRASTEIAPADVFVIAVPTPFEKDGHHTPDISYVLAAATQVAAVLKAGDTV
HHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEE
ILESTSPVGTTEQMRDLMAGLRPDLKFPGKTRETPDVSIAYCPERVLPGRILEELTNNDR
EEECCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEECCCCCCCHHHHHHHHCCCC
SIGGITPRCARKALAFYKRFVRGTCVTTDARSAEMTKLVENAYRDVNIAFANELSMVADR
CCCCCCHHHHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHCEEEEEHHHHHHHHHH
MGLDVWEVIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVAGAPDETPLIRTARNVNDGK
HCCCHHHHHHHHCCCCCEEEECCCCCCCCEEEEECCEEEEECCCCCCCHHHHHCCCCCHH
MHHVVKKATELVEAHPGEKVACLGLAFKANIDDFRESPARYVAASLARRFGSRVHIVEPY
HHHHHHHHHHHHHCCCCCEEEEEEEEEECCCHHHHHCHHHHHHHHHHHHHCCEEEEECCC
AGTLPREFEGTGAVQIDVDTALEDCGVLIVLVDHDVFKVIPLAERAGKVVYDTRGIWPDQ
CCCCCCCCCCCCEEEEEHHHHHHCCCEEEEEECCCHHHEEEHHHHCCCEEEECCCCCCCC
PGAESAGAGLRLAS
CCCCCCCCCCEECC
>Mature Secondary Structure
MMGDVKPDVCVVGLGYIGLPTAAIIARAGCRVLGLDVSQKVVDTINRGEIHIEEVDLDGL
CCCCCCCCEEEEECCHHHHHHHHHHHHCCCEEEECCHHHHHHHHCCCCEEEEEEECHHHH
VHGVVQRGLLRASTEIAPADVFVIAVPTPFEKDGHHTPDISYVLAAATQVAAVLKAGDTV
HHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEE
ILESTSPVGTTEQMRDLMAGLRPDLKFPGKTRETPDVSIAYCPERVLPGRILEELTNNDR
EEECCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEECCCCCCCHHHHHHHHCCCC
SIGGITPRCARKALAFYKRFVRGTCVTTDARSAEMTKLVENAYRDVNIAFANELSMVADR
CCCCCCHHHHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHCEEEEEHHHHHHHHHH
MGLDVWEVIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVAGAPDETPLIRTARNVNDGK
HCCCHHHHHHHHCCCCCEEEECCCCCCCCEEEEECCEEEEECCCCCCCHHHHHCCCCCHH
MHHVVKKATELVEAHPGEKVACLGLAFKANIDDFRESPARYVAASLARRFGSRVHIVEPY
HHHHHHHHHHHHHCCCCCEEEEEEEEEECCCHHHHHCHHHHHHHHHHHHHCCEEEEECCC
AGTLPREFEGTGAVQIDVDTALEDCGVLIVLVDHDVFKVIPLAERAGKVVYDTRGIWPDQ
CCCCCCCCCCCCEEEEEHHHHHHCCCEEEEEECCCHHHEEEHHHHCCCEEEECCCCCCCC
PGAESAGAGLRLAS
CCCCCCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: UDP-N-acetyl-D-mannosamine; NAD [C]

Specific reaction: UDP-N-acetyl-D-mannosamine + NAD = UDP-N-acetyl-D-mannosaminuronic-acid + NADH + Proton [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]