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The map label for this gene is rluE [H]

Identifier: 87201125

GI number: 87201125

Start: 3327559

End: 3328107

Strand: Reverse

Name: rluE [H]

Synonym: Saro_3113

Alternate gene names: 87201125

Gene position: 3328107-3327559 (Counterclockwise)

Preceding gene: 87201126

Following gene: 87201121

Centisome position: 93.44

GC content: 69.03

Gene sequence:

>549_bases
ATGGCCCTCATCCTGTTCAACAAGCCCTATGGCGTGCTCTGCCAGTTCACCGACGAACGCACCGGTCCGCCCCGGCCGAC
GCTTGCGGACCATATCCACCGTCCCGGCGTCTATCCGGCCGGCCGCCTCGACCATGACAGCGAGGGTCTCCTCCTCCTCA
CCGACGATGGCCGCCTCCAGGCCCGTATCGCGGACCCGCGCCACAAGATGCCCAAGACCTACCTCGCGCAGGTCGAAGGC
GTACCCGACGAAGCCGCGCTTGCTTCCTTGCGCAAGGGCGTGCGCCTCAAGGACGGCATGACGCTTCCGGCCGAAGCCGC
GCTGATCGAAGATCCGGCGCTTTGGCCGCGCGATCCGCCGATCCGCGTGCGCAAGACCGTGCCCGACAGTTGGCTGCGCC
TGACCATCCGCGAAGGCCGCAACCGCCAGGTCCGGCGCATGACCGCCGCCGTGGGCCATCCCACGCTGCGCTTGGTCCGC
TGGCAGGTCGGCGACTGGTCGCTCGATGGCTTGCGGCCTGGCGAATGGCGCGAAGTCGAGGTCCGCTGA

Upstream 100 bases:

>100_bases
TCGTGTCCGGTGCCATGCGCGCCTATTTCGCGCGCCTCGGGCAACGGCCCGCTTTCCAGCGCGCGATTGCCGCGCAGGGA
TCGCCGGCCTAAGCCGGGGC

Downstream 100 bases:

>100_bases
TGCGGCGGCGGCCTATCGCCGCGATCTGACAGCCGGAGTTTCACCCTGTCCGGCAAGGCCTTCGGCTTGCGCGATCGCCG
CCTTGGCGATCGGCTCCATC

Product: ribosomal large subunit pseudouridine synthase E

Products: NA

Alternate protein names: rRNA pseudouridylate synthase E; rRNA-uridine isomerase E [H]

Number of amino acids: Translated: 182; Mature: 181

Protein sequence:

>182_residues
MALILFNKPYGVLCQFTDERTGPPRPTLADHIHRPGVYPAGRLDHDSEGLLLLTDDGRLQARIADPRHKMPKTYLAQVEG
VPDEAALASLRKGVRLKDGMTLPAEAALIEDPALWPRDPPIRVRKTVPDSWLRLTIREGRNRQVRRMTAAVGHPTLRLVR
WQVGDWSLDGLRPGEWREVEVR

Sequences:

>Translated_182_residues
MALILFNKPYGVLCQFTDERTGPPRPTLADHIHRPGVYPAGRLDHDSEGLLLLTDDGRLQARIADPRHKMPKTYLAQVEG
VPDEAALASLRKGVRLKDGMTLPAEAALIEDPALWPRDPPIRVRKTVPDSWLRLTIREGRNRQVRRMTAAVGHPTLRLVR
WQVGDWSLDGLRPGEWREVEVR
>Mature_181_residues
ALILFNKPYGVLCQFTDERTGPPRPTLADHIHRPGVYPAGRLDHDSEGLLLLTDDGRLQARIADPRHKMPKTYLAQVEGV
PDEAALASLRKGVRLKDGMTLPAEAALIEDPALWPRDPPIRVRKTVPDSWLRLTIREGRNRQVRRMTAAVGHPTLRLVRW
QVGDWSLDGLRPGEWREVEVR

Specific function: Responsible for synthesis of pseudouridine from uracil- 2457 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase rsuA family [H]

Homologues:

Organism=Escherichia coli, GI87081838, Length=178, Percent_Identity=62.3595505617977, Blast_Score=216, Evalue=7e-58,
Organism=Escherichia coli, GI1788510, Length=179, Percent_Identity=37.9888268156425, Blast_Score=99, Evalue=1e-22,
Organism=Escherichia coli, GI1787524, Length=185, Percent_Identity=35.6756756756757, Blast_Score=82, Evalue=2e-17,
Organism=Escherichia coli, GI1790453, Length=180, Percent_Identity=30, Blast_Score=77, Evalue=5e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496 [H]

Pfam domain/function: PF00849 PseudoU_synth_2 [H]

EC number: NA

Molecular weight: Translated: 20625; Mature: 20494

Theoretical pI: Translated: 10.21; Mature: 10.21

Prosite motif: PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALILFNKPYGVLCQFTDERTGPPRPTLADHIHRPGVYPAGRLDHDSEGLLLLTDDGRLQ
CEEEEEECCCCEEEEECCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCEEEEECCCEEE
ARIADPRHKMPKTYLAQVEGVPDEAALASLRKGVRLKDGMTLPAEAALIEDPALWPRDPP
EEECCCHHHCCHHHHHHHCCCCCHHHHHHHHCCCEECCCCCCCCCCEEECCCCCCCCCCC
IRVRKTVPDSWLRLTIREGRNRQVRRMTAAVGHPTLRLVRWQVGDWSLDGLRPGEWREVE
EEEEECCCCCEEEEEEECCCCCHHHHHHHHHCCCEEEEEEEECCCEECCCCCCCCCEEEE
VR
EC
>Mature Secondary Structure 
ALILFNKPYGVLCQFTDERTGPPRPTLADHIHRPGVYPAGRLDHDSEGLLLLTDDGRLQ
EEEEEECCCCEEEEECCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCEEEEECCCEEE
ARIADPRHKMPKTYLAQVEGVPDEAALASLRKGVRLKDGMTLPAEAALIEDPALWPRDPP
EEECCCHHHCCHHHHHHHCCCCCHHHHHHHHCCCEECCCCCCCCCCEEECCCCCCCCCCC
IRVRKTVPDSWLRLTIREGRNRQVRRMTAAVGHPTLRLVRWQVGDWSLDGLRPGEWREVE
EEEEECCCCCEEEEEEECCCCCHHHHHHHHHCCCEEEEEEEECCCEECCCCCCCCCEEEE
VR
EC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]