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The map label for this gene is 87201105

Identifier: 87201105

GI number: 87201105

Start: 3313271

End: 3314119

Strand: Reverse

Name: 87201105

Synonym: Saro_3093

Alternate gene names: NA

Gene position: 3314119-3313271 (Counterclockwise)

Preceding gene: 87201106

Following gene: 87201104

Centisome position: 93.05

GC content: 65.72

Gene sequence:

>849_bases
ATGACCGAGATCACCGTCGCCGCCCTGCAGCTCCCTCTCGGGGGAGGCGAAGCCGAGAACATCCCAGCCGTCGCCGCCCT
GGTCGAAGAGGCCGCACGCGGCGGTGCGCAGATCGTCCTGCCGCCCGAACTGTTCGCGGGCCCCTATTTCTGCAAGGTCG
AGGACGAGGGCCTGTTCGGCCTTGCCTATACTCTGGACGAGGACCCTTCGGTCCGCGAGATGCGCAAGCTGGCCAAGGCG
CATGGCATCGCCATCCCGACGAGCTTCTTCGAGCGCGACGGGCACCACTACTACAACACCCTGGCGATGATCGGCACGGA
CGGCGAAGTCATGGGGATCTATCGCAAGAGCCACATCCCCGATGGCCCGGGCTATGAAGAGAAGTACTATTTCCGCCCTG
GAAATACGGGCTTCAAGGTGTGGGACGTGTTCGGCGCGCGCATCGGCGTGGGCGTCTGCTGGGACCAGTGGTATCCGGAA
TGCGCGCGCGCGATGGCGCTGATGGGCGCGGAACTGCTGTTCTACCCCACCGCCATCGGGTCGGAACCCTATGACGCCGA
TCTCGACACCAGCCGCATGTGGCGCCGGGCAATGCTGGGCCATGCGGTGTCGAACTGCATGCCGGTGATCGCCGCCAACC
GTATTGGCACGGAAGATGGCCAGAAGTTCTACGGCCACAGCTTCATCAGTGACGAATGGGGCGATTTCCTGGGCGAGTTC
GGCGCCGCCGAGACCGGCGTGCTCACCGCCACGATCGACCTGGCGCGTGCGGCAAGGCACCGTGCCGGCATGGGCTTCTT
CCGCGACCGCCGGCCGCAGCTCTATGCGCGCCTCGCCGAGGACAAGTGA

Upstream 100 bases:

>100_bases
ATTGGCGAAAGCGACCGGATGACGCTGCTGTTCCGCAAGCGCCCGTGATTCGGAACGGCAACGGTTGAATTGGCAAGGCG
GGGGGCCTAGTTCGGCTGCC

Downstream 100 bases:

>100_bases
ACCGATATCTCATCGCGCTGGGATCAAATCAGCGCCACCCCCGTCATGGCCTGCCGTCCATGATCCTGCGGGCAGCGGTG
GACGAACTGCGCGGTGAAGG

Product: Nitrilase/cyanide hydratase and apolipoprotein N-acyltransferase

Products: NA

Alternate protein names: D-N-alpha-carbamilase [H]

Number of amino acids: Translated: 282; Mature: 281

Protein sequence:

>282_residues
MTEITVAALQLPLGGGEAENIPAVAALVEEAARGGAQIVLPPELFAGPYFCKVEDEGLFGLAYTLDEDPSVREMRKLAKA
HGIAIPTSFFERDGHHYYNTLAMIGTDGEVMGIYRKSHIPDGPGYEEKYYFRPGNTGFKVWDVFGARIGVGVCWDQWYPE
CARAMALMGAELLFYPTAIGSEPYDADLDTSRMWRRAMLGHAVSNCMPVIAANRIGTEDGQKFYGHSFISDEWGDFLGEF
GAAETGVLTATIDLARAARHRAGMGFFRDRRPQLYARLAEDK

Sequences:

>Translated_282_residues
MTEITVAALQLPLGGGEAENIPAVAALVEEAARGGAQIVLPPELFAGPYFCKVEDEGLFGLAYTLDEDPSVREMRKLAKA
HGIAIPTSFFERDGHHYYNTLAMIGTDGEVMGIYRKSHIPDGPGYEEKYYFRPGNTGFKVWDVFGARIGVGVCWDQWYPE
CARAMALMGAELLFYPTAIGSEPYDADLDTSRMWRRAMLGHAVSNCMPVIAANRIGTEDGQKFYGHSFISDEWGDFLGEF
GAAETGVLTATIDLARAARHRAGMGFFRDRRPQLYARLAEDK
>Mature_281_residues
TEITVAALQLPLGGGEAENIPAVAALVEEAARGGAQIVLPPELFAGPYFCKVEDEGLFGLAYTLDEDPSVREMRKLAKAH
GIAIPTSFFERDGHHYYNTLAMIGTDGEVMGIYRKSHIPDGPGYEEKYYFRPGNTGFKVWDVFGARIGVGVCWDQWYPEC
ARAMALMGAELLFYPTAIGSEPYDADLDTSRMWRRAMLGHAVSNCMPVIAANRIGTEDGQKFYGHSFISDEWGDFLGEFG
AAETGVLTATIDLARAARHRAGMGFFRDRRPQLYARLAEDK

Specific function: The enzyme catalyzes the hydrolysis of N-carbamoyl-D- amino acids to the corresponding which are useful intermediates in the preparation of beta-lactam antibiotics. Industrial production of beta-lactam antibiotics is now being developed using this enzyme

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Homo sapiens, GI9910460, Length=285, Percent_Identity=31.2280701754386, Blast_Score=117, Evalue=1e-26,
Organism=Homo sapiens, GI7706509, Length=298, Percent_Identity=29.5302013422819, Blast_Score=111, Evalue=7e-25,
Organism=Homo sapiens, GI297632350, Length=286, Percent_Identity=29.020979020979, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI297632348, Length=286, Percent_Identity=29.020979020979, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI5031947, Length=286, Percent_Identity=29.020979020979, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI297632346, Length=188, Percent_Identity=30.3191489361702, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17533173, Length=277, Percent_Identity=29.6028880866426, Blast_Score=107, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17556280, Length=288, Percent_Identity=25.3472222222222, Blast_Score=81, Evalue=5e-16,
Organism=Saccharomyces cerevisiae, GI6323383, Length=292, Percent_Identity=25, Blast_Score=74, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6322335, Length=309, Percent_Identity=22.9773462783171, Blast_Score=72, Evalue=1e-13,
Organism=Drosophila melanogaster, GI21358471, Length=276, Percent_Identity=29.3478260869565, Blast_Score=111, Evalue=5e-25,
Organism=Drosophila melanogaster, GI17933642, Length=285, Percent_Identity=27.3684210526316, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI21355835, Length=281, Percent_Identity=24.5551601423488, Blast_Score=79, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003010 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: =3.5.1.77 [H]

Molecular weight: Translated: 31094; Mature: 30962

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEITVAALQLPLGGGEAENIPAVAALVEEAARGGAQIVLPPELFAGPYFCKVEDEGLFG
CCCEEEEEEEEECCCCCCCCCHHHHHHHHHHHCCCCEEEECHHHHCCCEEEEEECCCEEE
LAYTLDEDPSVREMRKLAKAHGIAIPTSFFERDGHHYYNTLAMIGTDGEVMGIYRKSHIP
EEEEECCCCCHHHHHHHHHHCCCCCCHHHHHCCCCEEEEEEEEECCCCCEEEEEECCCCC
DGPGYEEKYYFRPGNTGFKVWDVFGARIGVGVCWDQWYPECARAMALMGAELLFYPTAIG
CCCCCCCEEEECCCCCCCCCEEEHHHHHCCCHHHHHHHHHHHHHHHHHCCHHEEEEHHCC
SEPYDADLDTSRMWRRAMLGHAVSNCMPVIAANRIGTEDGQKFYGHSFISDEWGDFLGEF
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCCHHHHHHHHHH
GAAETGVLTATIDLARAARHRAGMGFFRDRRPQLYARLAEDK
CCCCCCEEEEHHHHHHHHHHHCCCCHHHCCCHHHHHHHHCCC
>Mature Secondary Structure 
TEITVAALQLPLGGGEAENIPAVAALVEEAARGGAQIVLPPELFAGPYFCKVEDEGLFG
CCEEEEEEEEECCCCCCCCCHHHHHHHHHHHCCCCEEEECHHHHCCCEEEEEECCCEEE
LAYTLDEDPSVREMRKLAKAHGIAIPTSFFERDGHHYYNTLAMIGTDGEVMGIYRKSHIP
EEEEECCCCCHHHHHHHHHHCCCCCCHHHHHCCCCEEEEEEEEECCCCCEEEEEECCCCC
DGPGYEEKYYFRPGNTGFKVWDVFGARIGVGVCWDQWYPECARAMALMGAELLFYPTAIG
CCCCCCCEEEECCCCCCCCCEEEHHHHHCCCHHHHHHHHHHHHHHHHHCCHHEEEEHHCC
SEPYDADLDTSRMWRRAMLGHAVSNCMPVIAANRIGTEDGQKFYGHSFISDEWGDFLGEF
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCCHHHHHHHHHH
GAAETGVLTATIDLARAARHRAGMGFFRDRRPQLYARLAEDK
CCCCCCEEEEHHHHHHHHHHHCCCCHHHCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9648217; 10903946 [H]