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The map label for this gene is fliA [H]

Identifier: 87201086

GI number: 87201086

Start: 3293161

End: 3293895

Strand: Reverse

Name: fliA [H]

Synonym: Saro_3074

Alternate gene names: 87201086

Gene position: 3293895-3293161 (Counterclockwise)

Preceding gene: 87201088

Following gene: 87201085

Centisome position: 92.48

GC content: 66.94

Gene sequence:

>735_bases
ATGAAGCACGATCACCGCGGCTTTGCCGCAGCACAGGCCTATCGCAAGGACGAGACAGCGGATCGCATTCGGCGGTTTCT
GCCCATGGTCAAGCGGCTCGCCTGGCACGTCCACGGCTCGGGCCGGTCGGGCATCGAGATCGAGGATCTGATCCAGGCCG
GCCTTGTCGCGCTTACCGAATGCGCGCAGCGCCACGATGGTCCGACCGAGGATGGCTTCGCCGCCTATGCCAAGCTGCGG
GTGAGGGGCGCGATGGTCGACCTCGTGCGCCGGTCGACGCCGCTGTCGCGCTCGGCAACCGAACGGCGCCGCGTCCTGCG
CGAGAAGACCGCGACGCTCGCGACCGAACTGGGCCGCCCGCCGAGCGAAGCCGAGCTTGCCCGCGCGGTGGGCCTGACCG
AGCGCGAACTGGCCGACCAGCGTGCCGCCGACGAACCCCTGCGTTTCGAGGCGATCGACGATGCCTATTCCGACAGCAAC
TCGGCGTTTGCCGACGACCGGCCCGACAGCTTCGACATGCTGGCCGATGCGGAAATGCGCGGAGCCGTCGCGGCTGCCAT
CGCCGGACTGCCGGAGCGGTTGCAGTTGATCATCCAGCTCTACTTCGTCGAGGAACTTAACCTTGCGGAGATCGCCGAAG
TGCTCGGCGTGAGCATCCCGCGCGTGCATCAGTTGAAGGCCCAGGCCATCGACAAGCTGCGCATGGCGCTCGGAGAGGGG
TACGATATTCTCTAA

Upstream 100 bases:

>100_bases
TCGATGAATCCTGTGGTTCGCCTCAGCCCGAGAGGCGCATCGGCGGCGAGGCGCCGCAACCTGCTCATCACGAGATGCCG
ATCCCCGAGGGAATTGCCGC

Downstream 100 bases:

>100_bases
GCGGCTTGCCATCGTTCCCAATAGCGTTTAACCGATCGGTTAAACGCTATTGGGAGAGGCAATGATGGCGCTCAAGACAC
GCATCACCCAGATGCTCGGC

Product: sigma 28 (flagella/sporulation)

Products: NA

Alternate protein names: Sigma-28; Sigma-F factor [H]

Number of amino acids: Translated: 244; Mature: 244

Protein sequence:

>244_residues
MKHDHRGFAAAQAYRKDETADRIRRFLPMVKRLAWHVHGSGRSGIEIEDLIQAGLVALTECAQRHDGPTEDGFAAYAKLR
VRGAMVDLVRRSTPLSRSATERRRVLREKTATLATELGRPPSEAELARAVGLTERELADQRAADEPLRFEAIDDAYSDSN
SAFADDRPDSFDMLADAEMRGAVAAAIAGLPERLQLIIQLYFVEELNLAEIAEVLGVSIPRVHQLKAQAIDKLRMALGEG
YDIL

Sequences:

>Translated_244_residues
MKHDHRGFAAAQAYRKDETADRIRRFLPMVKRLAWHVHGSGRSGIEIEDLIQAGLVALTECAQRHDGPTEDGFAAYAKLR
VRGAMVDLVRRSTPLSRSATERRRVLREKTATLATELGRPPSEAELARAVGLTERELADQRAADEPLRFEAIDDAYSDSN
SAFADDRPDSFDMLADAEMRGAVAAAIAGLPERLQLIIQLYFVEELNLAEIAEVLGVSIPRVHQLKAQAIDKLRMALGEG
YDIL
>Mature_244_residues
MKHDHRGFAAAQAYRKDETADRIRRFLPMVKRLAWHVHGSGRSGIEIEDLIQAGLVALTECAQRHDGPTEDGFAAYAKLR
VRGAMVDLVRRSTPLSRSATERRRVLREKTATLATELGRPPSEAELARAVGLTERELADQRAADEPLRFEAIDDAYSDSN
SAFADDRPDSFDMLADAEMRGAVAAAIAGLPERLQLIIQLYFVEELNLAEIAEVLGVSIPRVHQLKAQAIDKLRMALGEG
YDIL

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This alternative sigma factor is specific for the flagellin gene (fliC) expression [H]

COG id: COG1191

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1788231, Length=224, Percent_Identity=34.375, Blast_Score=119, Evalue=1e-28,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR012845
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 27030; Mature: 27030

Theoretical pI: Translated: 5.70; Mature: 5.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKHDHRGFAAAQAYRKDETADRIRRFLPMVKRLAWHVHGSGRSGIEIEDLIQAGLVALTE
CCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHH
CAQRHDGPTEDGFAAYAKLRVRGAMVDLVRRSTPLSRSATERRRVLREKTATLATELGRP
HHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCC
PSEAELARAVGLTERELADQRAADEPLRFEAIDDAYSDSNSAFADDRPDSFDMLADAEMR
CCHHHHHHHHCCHHHHHHHHHCCCCCCEEHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHH
GAVAAAIAGLPERLQLIIQLYFVEELNLAEIAEVLGVSIPRVHQLKAQAIDKLRMALGEG
HHHHHHHHCCHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCC
YDIL
CCCC
>Mature Secondary Structure
MKHDHRGFAAAQAYRKDETADRIRRFLPMVKRLAWHVHGSGRSGIEIEDLIQAGLVALTE
CCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHH
CAQRHDGPTEDGFAAYAKLRVRGAMVDLVRRSTPLSRSATERRRVLREKTATLATELGRP
HHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCC
PSEAELARAVGLTERELADQRAADEPLRFEAIDDAYSDSNSAFADDRPDSFDMLADAEMR
CCHHHHHHHHCCHHHHHHHHHCCCCCCEEHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHH
GAVAAAIAGLPERLQLIIQLYFVEELNLAEIAEVLGVSIPRVHQLKAQAIDKLRMALGEG
HHHHHHHHCCHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCC
YDIL
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1560774; 10984043 [H]