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The map label for this gene is amiD [H]

Identifier: 87201063

GI number: 87201063

Start: 3266537

End: 3267241

Strand: Direct

Name: amiD [H]

Synonym: Saro_3051

Alternate gene names: 87201063

Gene position: 3266537-3267241 (Clockwise)

Preceding gene: 87201062

Following gene: 87201064

Centisome position: 91.72

GC content: 66.95

Gene sequence:

>705_bases
ATGCGCCGCTGGCTCGTCCACCGCGAGATACCCTCTCCGAACTGGAACGAGCGCAAGCTGCCGGTCAGCATGGTCGTCCT
CCACTATACCGGCATGGAGACCGCCGATGCCGCACTGGAACGCCTGTGCGATCCGGCGGCGGAAGTGTCGGCGCACTACA
TGATCGACGAGGACGGGACCGTCACCCAGCTCGTCGACGAGGAAAAGCGCGCCTGGCACGCCGGGCGCTCTTCCTGGCGC
GGGATCACCGACGTCAATTCGGCCAGCATCGGGATCGAGCTGGTCAACCCCGGCCACGAGTTCGGCTACCGCCCCTTCCC
GGATGCGCAGATCGAGGCGCTGCTGCCACTGCTGGCGGACATCGTGAAGCGCCATGACATCCCGCGCGCCAACGTCGTCG
GCCATTCGGATGTCGCTCCGGCGCGCAAGGACGATCCGGGCGAGCTGTTCCCCTGGGAACTCCTCGCCCGCCACCGCCTT
TGCCTGCCGACGCCGAAGCTGTCCATGCGGCTTCTCTACGACAACGAGGGCGCCTTTTTCCTCGCCCTCGAACGCTTTGG
CTACGACATTACCGATCCGCCGGCCGCTGTGCGCGCGTTCCAGCGGCGCTGGCGACCGCAGCGGATCGACGGGCAGGTCG
ACGGGCAGATCGGCGCGCTGCTCTTCGAACTCCTGTTGGAACGCGATCTCGGGCATGCTAGGTGA

Upstream 100 bases:

>100_bases
CTCGCCGCTGCGGTCGGCGGCGGCGTACAGGTCCACATCGCGCCCGACAGCCTCGTCCTCGGCGCGGTCCTTCCCTCGGC
CTGACGGAACGGGGGGCACC

Downstream 100 bases:

>100_bases
CCCGTCGCCAGGGGGCCGGGCAGCCGCGTCATCTCACGATGCCGAGGAAAGTCCGGGCTCCACGAAACAAGGGTGGCGGG
TAACGCCCGCCGGGTCGGGC

Product: negative regulator of AmpC, AmpD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 234; Mature: 234

Protein sequence:

>234_residues
MRRWLVHREIPSPNWNERKLPVSMVVLHYTGMETADAALERLCDPAAEVSAHYMIDEDGTVTQLVDEEKRAWHAGRSSWR
GITDVNSASIGIELVNPGHEFGYRPFPDAQIEALLPLLADIVKRHDIPRANVVGHSDVAPARKDDPGELFPWELLARHRL
CLPTPKLSMRLLYDNEGAFFLALERFGYDITDPPAAVRAFQRRWRPQRIDGQVDGQIGALLFELLLERDLGHAR

Sequences:

>Translated_234_residues
MRRWLVHREIPSPNWNERKLPVSMVVLHYTGMETADAALERLCDPAAEVSAHYMIDEDGTVTQLVDEEKRAWHAGRSSWR
GITDVNSASIGIELVNPGHEFGYRPFPDAQIEALLPLLADIVKRHDIPRANVVGHSDVAPARKDDPGELFPWELLARHRL
CLPTPKLSMRLLYDNEGAFFLALERFGYDITDPPAAVRAFQRRWRPQRIDGQVDGQIGALLFELLLERDLGHAR
>Mature_234_residues
MRRWLVHREIPSPNWNERKLPVSMVVLHYTGMETADAALERLCDPAAEVSAHYMIDEDGTVTQLVDEEKRAWHAGRSSWR
GITDVNSASIGIELVNPGHEFGYRPFPDAQIEALLPLLADIVKRHDIPRANVVGHSDVAPARKDDPGELFPWELLARHRL
CLPTPKLSMRLLYDNEGAFFLALERFGYDITDPPAAVRAFQRRWRPQRIDGQVDGQIGALLFELLLERDLGHAR

Specific function: Unknown

COG id: COG3023

COG function: function code V; Negative regulator of beta-lactamase expression

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family [H]

Homologues:

Organism=Escherichia coli, GI1787092, Length=233, Percent_Identity=37.3390557939914, Blast_Score=132, Evalue=3e-32,
Organism=Escherichia coli, GI1786300, Length=177, Percent_Identity=35.0282485875706, Blast_Score=80, Evalue=9e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002502
- InterPro:   IPR002477 [H]

Pfam domain/function: PF01510 Amidase_2 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 26599; Mature: 26599

Theoretical pI: Translated: 6.04; Mature: 6.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRWLVHREIPSPNWNERKLPVSMVVLHYTGMETADAALERLCDPAAEVSAHYMIDEDGT
CCCCEEECCCCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHCCCHHHCCEEEEECCCCC
VTQLVDEEKRAWHAGRSSWRGITDVNSASIGIELVNPGHEFGYRPFPDAQIEALLPLLAD
HHHHHHHHHHHHHHCHHHCCCCCCCCCCEEEEEEECCCHHCCCCCCCCHHHHHHHHHHHH
IVKRHDIPRANVVGHSDVAPARKDDPGELFPWELLARHRLCLPTPKLSMRLLYDNEGAFF
HHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCEEEEEEECCCCEE
LALERFGYDITDPPAAVRAFQRRWRPQRIDGQVDGQIGALLFELLLERDLGHAR
EEEHHHCCCCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MRRWLVHREIPSPNWNERKLPVSMVVLHYTGMETADAALERLCDPAAEVSAHYMIDEDGT
CCCCEEECCCCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHCCCHHHCCEEEEECCCCC
VTQLVDEEKRAWHAGRSSWRGITDVNSASIGIELVNPGHEFGYRPFPDAQIEALLPLLAD
HHHHHHHHHHHHHHCHHHCCCCCCCCCCEEEEEEECCCHHCCCCCCCCHHHHHHHHHHHH
IVKRHDIPRANVVGHSDVAPARKDDPGELFPWELLARHRLCLPTPKLSMRLLYDNEGAFF
HHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCEEEEEEECCCCEE
LALERFGYDITDPPAAVRAFQRRWRPQRIDGQVDGQIGALLFELLLERDLGHAR
EEEHHHCCCCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]