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The map label for this gene is rpoH [H]

Identifier: 87201057

GI number: 87201057

Start: 3260186

End: 3261085

Strand: Reverse

Name: rpoH [H]

Synonym: Saro_3045

Alternate gene names: 87201057

Gene position: 3261085-3260186 (Counterclockwise)

Preceding gene: 87201058

Following gene: 87201055

Centisome position: 91.56

GC content: 62.56

Gene sequence:

>900_bases
GTGAGTGAGGAAAAGACCGCGCTGACCGTCCCCGCGCTGGGTGGGGAAGCCAGCCTCAACCGCTATCTCTCGGAAATCCG
CAAGTTTCCCGTGCTGACGGCAGAGCAGGAATACATGCTCGCCAAGCGCTTTCAGGAACATCAGGACCCCGAGGCCGCCG
CACAACTGGTGACGAGCCACCTGCGGCTCGTGGCGAAGATCGCCATGGGCTATCGCGGCTATGGCCTGCCGGTGAGCGAG
CTGATCAGCGAGGGCAACATCGGCCTGATGCAGGGCGTCAAGAAGTTCGAGCCGGACCGGGGTTTCCGTCTGGCGACTTA
CGCGATGTGGTGGATCAAGGCCTCGATGCAGGAATTCATCCTGCGCAGCTGGTCGCTCGTGAAGATGGGCACCACCGCCG
CGCAGAAGAAGCTGTTCTTCAACCTGCGGCGAATGAAGAAGAACCTCGAGGCTTTCGAGGATTCCGACCTTCATCCCGAC
GACGTGAGGAAGATCGCGACCGACCTCGGCGTACCCGAGCAGGAAGTGGTCAACATGAACCGGCGCATGATGATGGGCGG
CGATGCGTCGCTCAACGTCTCGATGCGCGAGGACGGCGAAGGATCGTGGCAGGACTGGTTGACGGACGACCGTCCGCTCC
AGGATGAAACCGTGGCCGACGCCGAGGAAGCGCAGTATCGCCACGAACTGCTGGTCGAGGCGATGGAAAGCCTCAACGAG
CGCGAGCGCCACATCCTGACCGACCGCAGGCTGATCGACGATCCCAAGACGCTCGAGGAACTGAGCCAGGTCTACAACGT
CAGCCGCGAACGCGTGCGTCAGATCGAGGTGCGCGCCTTCGAGAAGCTGCAGAAGGCGATCCAGCGCATCGCGGTGGAGC
GCAAGCTCCTGCCGGCATAA

Upstream 100 bases:

>100_bases
GGAGTCGCCCGAAAGGCGGCTTCGCAATGTGTGGTCCCGCCGCCGGTCGCCCATCAGGGGGCCGACAAAGGGGAACCGAC
GCGACGAAAGGAATATTCAG

Downstream 100 bases:

>100_bases
GGATCGGGTGGATCGGATGAGCGGGGCAAGGTGAATTGCTATTTTGCCCCGTCGTCCTAGAATGTACCCAGTTTGCCGCC
CCGGCGGCGCGAACAGCGAG

Product: RNA polymerase factor sigma-32

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 299; Mature: 298

Protein sequence:

>299_residues
MSEEKTALTVPALGGEASLNRYLSEIRKFPVLTAEQEYMLAKRFQEHQDPEAAAQLVTSHLRLVAKIAMGYRGYGLPVSE
LISEGNIGLMQGVKKFEPDRGFRLATYAMWWIKASMQEFILRSWSLVKMGTTAAQKKLFFNLRRMKKNLEAFEDSDLHPD
DVRKIATDLGVPEQEVVNMNRRMMMGGDASLNVSMREDGEGSWQDWLTDDRPLQDETVADAEEAQYRHELLVEAMESLNE
RERHILTDRRLIDDPKTLEELSQVYNVSRERVRQIEVRAFEKLQKAIQRIAVERKLLPA

Sequences:

>Translated_299_residues
MSEEKTALTVPALGGEASLNRYLSEIRKFPVLTAEQEYMLAKRFQEHQDPEAAAQLVTSHLRLVAKIAMGYRGYGLPVSE
LISEGNIGLMQGVKKFEPDRGFRLATYAMWWIKASMQEFILRSWSLVKMGTTAAQKKLFFNLRRMKKNLEAFEDSDLHPD
DVRKIATDLGVPEQEVVNMNRRMMMGGDASLNVSMREDGEGSWQDWLTDDRPLQDETVADAEEAQYRHELLVEAMESLNE
RERHILTDRRLIDDPKTLEELSQVYNVSRERVRQIEVRAFEKLQKAIQRIAVERKLLPA
>Mature_298_residues
SEEKTALTVPALGGEASLNRYLSEIRKFPVLTAEQEYMLAKRFQEHQDPEAAAQLVTSHLRLVAKIAMGYRGYGLPVSEL
ISEGNIGLMQGVKKFEPDRGFRLATYAMWWIKASMQEFILRSWSLVKMGTTAAQKKLFFNLRRMKKNLEAFEDSDLHPDD
VRKIATDLGVPEQEVVNMNRRMMMGGDASLNVSMREDGEGSWQDWLTDDRPLQDETVADAEEAQYRHELLVEAMESLNER
ERHILTDRRLIDDPKTLEELSQVYNVSRERVRQIEVRAFEKLQKAIQRIAVERKLLPA

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of heat shock promoters [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. Sigma-32 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789871, Length=303, Percent_Identity=38.9438943894389, Blast_Score=192, Evalue=3e-50,
Organism=Escherichia coli, GI1789098, Length=272, Percent_Identity=31.6176470588235, Blast_Score=117, Evalue=7e-28,
Organism=Escherichia coli, GI1789448, Length=269, Percent_Identity=29.368029739777, Blast_Score=92, Evalue=6e-20,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012759
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 34473; Mature: 34342

Theoretical pI: Translated: 5.55; Mature: 5.55

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEEKTALTVPALGGEASLNRYLSEIRKFPVLTAEQEYMLAKRFQEHQDPEAAAQLVTSH
CCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHH
LRLVAKIAMGYRGYGLPVSELISEGNIGLMQGVKKFEPDRGFRLATYAMWWIKASMQEFI
HHHHHHHHHCCCCCCCCHHHHHCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHH
LRSWSLVKMGTTAAQKKLFFNLRRMKKNLEAFEDSDLHPDDVRKIATDLGVPEQEVVNMN
HHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCHHHHHCCC
RRMMMGGDASLNVSMREDGEGSWQDWLTDDRPLQDETVADAEEAQYRHELLVEAMESLNE
HHEEECCCCEEEEEECCCCCCCHHHHCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHH
RERHILTDRRLIDDPKTLEELSQVYNVSRERVRQIEVRAFEKLQKAIQRIAVERKLLPA
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SEEKTALTVPALGGEASLNRYLSEIRKFPVLTAEQEYMLAKRFQEHQDPEAAAQLVTSH
CCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHH
LRLVAKIAMGYRGYGLPVSELISEGNIGLMQGVKKFEPDRGFRLATYAMWWIKASMQEFI
HHHHHHHHHCCCCCCCCHHHHHCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHH
LRSWSLVKMGTTAAQKKLFFNLRRMKKNLEAFEDSDLHPDDVRKIATDLGVPEQEVVNMN
HHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCHHHHHCCC
RRMMMGGDASLNVSMREDGEGSWQDWLTDDRPLQDETVADAEEAQYRHELLVEAMESLNE
HHEEECCCCEEEEEECCCCCCCHHHHCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHH
RERHILTDRRLIDDPKTLEELSQVYNVSRERVRQIEVRAFEKLQKAIQRIAVERKLLPA
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7501460 [H]