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The map label for this gene is nasA [H]

Identifier: 87200982

GI number: 87200982

Start: 3189683

End: 3190936

Strand: Reverse

Name: nasA [H]

Synonym: Saro_2970

Alternate gene names: 87200982

Gene position: 3190936-3189683 (Counterclockwise)

Preceding gene: 87200984

Following gene: 87200981

Centisome position: 89.59

GC content: 66.91

Gene sequence:

>1254_bases
ATGGCATCGTCCGCAATGACACTGAACACACCCAGAATTCCGGTCGGAAAGTCGTTCTGGCAATCCGGCCACCGCCCGAC
GCTGTTCGCGGCGTTCCTCTATTTCGACATCGCCTTCATGGTCTGGGTGATCCTCGGCCCGCTGGCGCCGATCATCTCGA
AGGAGCTGGGGCTGGACCCCGCACAGAAGGGGATGATGGTGGCGGTGCCAACGCTGGCGGGCGCGGTCATCCGCCTCGTC
AACGGGCTGCTGGTCGACCGCATCGGCCCCAAGCGCACGGGCATCATCAGCCAGGTGGTGGTCATCGCGGGCATGTTCGC
CGCGTGGTGGTTCGGGATTTCGTCCTACGAGGGGACGCTGGCGCTTGGCGTGATCCTCGGCTTTGCCGGGGCGAGCTTCG
CGGTCTCGCTGCCGCTGGCGAGCCGGTGGTATCCGCCCGAGCACCAGGGCAAGGCCATGGGCATTGCCGGCATGGGCAAT
TCGGGCACGGTCTTCGCCTCGCTCTTCGCGGCCTTGCTGGCCAAGTCGTTCGGCTGGACCAATGTCTTCGGCCTGGCGCT
GATCCCGCTGGTCGCGGTCTTCGCGTTCTATCTCGTCGCGGCCAAGGACAGCCCCGAACAACCGGAGCCCAAGCCGCTTT
CGGCCTATGCCTCGGTGCTGAAGGAGCAGGACGCATGGTGGTTCATGCTGTTCTACTTCGTGACCTTTGGCGGCTTCGTC
GGCCTGTCCAGTTCGCTGCCGACGTTCTTTGCCGACAGCTTTGGCGTGACGCCGGTGATCGCCGGGTATTGCACGGCGGC
GGCGGTGTTCATGGGCTCGATGCTGCGGCCGCTGGGTGGCGCGCTTGCCGACCGCTTCGGCGGCATCCGCACCCTTTCGG
TCGTCTACGTGGTGGCCGGTGCCCTGCTGGCGGTTATCGCCGCTGTTTCCACCCAGGGACTGGCGCTGGCCATTGCGCTG
TTCGTGGCGGTGATGGGGACGCTGGGCGTGGGCAACGGTGCGGTTTTCCAGCTCGTGCCGCAACGGTTCCGCAAGGAGAT
CGGTGTGATGACCGGGCTCGTAGGCTTCGGGGGCGGCGTTGGCGGTTTCTACCTCGCATCGTCGCTGGGCGCGATCAAGA
AGGCCACCGGCAGCGCCTCGCTCGGCTTCCTGATCTTCGCTGCCCTCGCGGTGGTCGCGCTGGTCGGCCTGACGGCAGTC
AAGCGCCGCTGGCGGACGACCTGGGGCGCAGCGCTGGGCGGCGCGCGCATCTGA

Upstream 100 bases:

>100_bases
CGCATCGCCCGATCCCGCTCCTCGCATGTACGGGCGCAACGCCCGCCCGCGTGAGGATCGGTTCGGAGAGCTTCCAGTCA
AGTCCAGGGAGGACAAGGCA

Downstream 100 bases:

>100_bases
CCGCTGACGTACCGGCGACCGGGGGGCGACACAGCCGCCCGGCGCCACCATGAGGGAATTCGATCGATGAAGACCAGGAT
CCTCGGCGCCGCCGCGATGG

Product: major facilitator transporter

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 417; Mature: 416

Protein sequence:

>417_residues
MASSAMTLNTPRIPVGKSFWQSGHRPTLFAAFLYFDIAFMVWVILGPLAPIISKELGLDPAQKGMMVAVPTLAGAVIRLV
NGLLVDRIGPKRTGIISQVVVIAGMFAAWWFGISSYEGTLALGVILGFAGASFAVSLPLASRWYPPEHQGKAMGIAGMGN
SGTVFASLFAALLAKSFGWTNVFGLALIPLVAVFAFYLVAAKDSPEQPEPKPLSAYASVLKEQDAWWFMLFYFVTFGGFV
GLSSSLPTFFADSFGVTPVIAGYCTAAAVFMGSMLRPLGGALADRFGGIRTLSVVYVVAGALLAVIAAVSTQGLALAIAL
FVAVMGTLGVGNGAVFQLVPQRFRKEIGVMTGLVGFGGGVGGFYLASSLGAIKKATGSASLGFLIFAALAVVALVGLTAV
KRRWRTTWGAALGGARI

Sequences:

>Translated_417_residues
MASSAMTLNTPRIPVGKSFWQSGHRPTLFAAFLYFDIAFMVWVILGPLAPIISKELGLDPAQKGMMVAVPTLAGAVIRLV
NGLLVDRIGPKRTGIISQVVVIAGMFAAWWFGISSYEGTLALGVILGFAGASFAVSLPLASRWYPPEHQGKAMGIAGMGN
SGTVFASLFAALLAKSFGWTNVFGLALIPLVAVFAFYLVAAKDSPEQPEPKPLSAYASVLKEQDAWWFMLFYFVTFGGFV
GLSSSLPTFFADSFGVTPVIAGYCTAAAVFMGSMLRPLGGALADRFGGIRTLSVVYVVAGALLAVIAAVSTQGLALAIAL
FVAVMGTLGVGNGAVFQLVPQRFRKEIGVMTGLVGFGGGVGGFYLASSLGAIKKATGSASLGFLIFAALAVVALVGLTAV
KRRWRTTWGAALGGARI
>Mature_416_residues
ASSAMTLNTPRIPVGKSFWQSGHRPTLFAAFLYFDIAFMVWVILGPLAPIISKELGLDPAQKGMMVAVPTLAGAVIRLVN
GLLVDRIGPKRTGIISQVVVIAGMFAAWWFGISSYEGTLALGVILGFAGASFAVSLPLASRWYPPEHQGKAMGIAGMGNS
GTVFASLFAALLAKSFGWTNVFGLALIPLVAVFAFYLVAAKDSPEQPEPKPLSAYASVLKEQDAWWFMLFYFVTFGGFVG
LSSSLPTFFADSFGVTPVIAGYCTAAAVFMGSMLRPLGGALADRFGGIRTLSVVYVVAGALLAVIAAVSTQGLALAIALF
VAVMGTLGVGNGAVFQLVPQRFRKEIGVMTGLVGFGGGVGGFYLASSLGAIKKATGSASLGFLIFAALAVVALVGLTAVK
RRWRTTWGAALGGARI

Specific function: May function as a nitrate transporter [H]

COG id: COG2223

COG function: function code P; Nitrate/nitrite transporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Nitrate/nitrite porter (TC 2.A.1.8) family [H]

Homologues:

Organism=Escherichia coli, GI1787475, Length=446, Percent_Identity=26.457399103139, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1787743, Length=437, Percent_Identity=23.7986270022883, Blast_Score=69, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004737 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 43431; Mature: 43300

Theoretical pI: Translated: 10.60; Mature: 10.60

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASSAMTLNTPRIPVGKSFWQSGHRPTLFAAFLYFDIAFMVWVILGPLAPIISKELGLDP
CCCCCCCCCCCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
AQKGMMVAVPTLAGAVIRLVNGLLVDRIGPKRTGIISQVVVIAGMFAAWWFGISSYEGTL
HHCCCEEEHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
ALGVILGFAGASFAVSLPLASRWYPPEHQGKAMGIAGMGNSGTVFASLFAALLAKSFGWT
HHHHHHHHCCCHHEEECCHHHCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCH
NVFGLALIPLVAVFAFYLVAAKDSPEQPEPKPLSAYASVLKEQDAWWFMLFYFVTFGGFV
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHH
GLSSSLPTFFADSFGVTPVIAGYCTAAAVFMGSMLRPLGGALADRFGGIRTLSVVYVVAG
CCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
ALLAVIAAVSTQGLALAIALFVAVMGTLGVGNGAVFQLVPQRFRKEIGVMTGLVGFGGGV
HHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCH
GGFYLASSLGAIKKATGSASLGFLIFAALAVVALVGLTAVKRRWRTTWGAALGGARI
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
ASSAMTLNTPRIPVGKSFWQSGHRPTLFAAFLYFDIAFMVWVILGPLAPIISKELGLDP
CCCCCCCCCCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
AQKGMMVAVPTLAGAVIRLVNGLLVDRIGPKRTGIISQVVVIAGMFAAWWFGISSYEGTL
HHCCCEEEHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
ALGVILGFAGASFAVSLPLASRWYPPEHQGKAMGIAGMGNSGTVFASLFAALLAKSFGWT
HHHHHHHHCCCHHEEECCHHHCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCH
NVFGLALIPLVAVFAFYLVAAKDSPEQPEPKPLSAYASVLKEQDAWWFMLFYFVTFGGFV
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHH
GLSSSLPTFFADSFGVTPVIAGYCTAAAVFMGSMLRPLGGALADRFGGIRTLSVVYVVAG
CCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
ALLAVIAAVSTQGLALAIALFVAVMGTLGVGNGAVFQLVPQRFRKEIGVMTGLVGFGGGV
HHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCH
GGFYLASSLGAIKKATGSASLGFLIFAALAVVALVGLTAVKRRWRTTWGAALGGARI
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7868621; 8969502; 9384377 [H]