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The map label for this gene is nasA [H]

Identifier: 87200978

GI number: 87200978

Start: 3182727

End: 3185312

Strand: Reverse

Name: nasA [H]

Synonym: Saro_2966

Alternate gene names: 87200978

Gene position: 3185312-3182727 (Counterclockwise)

Preceding gene: 87200979

Following gene: 87200977

Centisome position: 89.44

GC content: 68.25

Gene sequence:

>2586_bases
GTGACGATGAAGGACATCCGCACCACATGCGCCTACTGCGGTGTCGGCTGCGGTATCCGGGCGACGGTGACGGGCGACCG
GGCCGTTTCGATCAAGGGCGACCCGGAGCACCCCGCGAACTTCGGGCGCCTGTGCTCGAAGGGGACGCACCTGGGCGAAA
CGGTGGGACTTGAGGGGCGGCTGCTTCACCCGATGGTGGATGCAGAGCGCGCAACCTGGAACGAGGCGCTCGATCTTGCG
GCGGCGCGGATGCGCGAATGCATCGAGCGCCACGGCCCCGACAGCGTTGCCTTCTACGTTTCGGGACAACTGCTCACCGA
GGACTACTACGTCGCCAACAAGCTGATGAAGGGCTTCATCGGCAGCGGCAACATCGACACCAACAGCCGGCTGTGCATGG
CCAGCGCGGTGGCGGCTCATAACCGCGCCTTCGGCGAGGACCTGGTGCCATGCACCTACGACGACCTGGAGGCGGCCGAC
CTCGTCCTGCTGGTCGGCTCGAACACGGCGTGGTGCCACCCGGTGATCTGGCAGAGGATCGAAGCGGCTCGCTCGGCACG
CGGGACAAGGCTGGTCGTGATCGACCCGCGCCGTACGGAGACCGCCGAGCAGGCCGACCTGCACGTCGCGCTCGCGCCCG
ACGGCGACATCGCGCTGTTCAACGCCCTTCTGGCCGAGATGCGCGACCGCGATCTTCTGGACGAGGCCTTCATGGCCGAG
CGGTGCGCCGTGCCCGAGGGATACGTCGCCGGGCTCGATCCGCGCGGTCACGGGATAGATCCCGCGACGTTCCGCGCGCT
TGCAGACCTCGTCGCGGCCAGCCCGCGCATGGTGACACTGTTCAGCCAGGGGGCGAATCAGTCGCGCTGCGGGACCGACA
AGGGCAACGCGATCATCAACCTCCACCTTGCGCTTGGCCGGATCAGCCAGACCGGTGCGGGGCCGTTCTCGATCACCGGC
CAGCCCAATGCGATGGGCGGACGCGAAGTTGGCGGGCTGGCGAACATGCTTGCGTGTCACCTGGGCTTTTCGTCCGAGGA
GCGCGCGGATGCGGCGATCTTCTGGAACGCGCCCAATATCTGCGCAGGGCCGGGGCTGAAGGCGGTGGACCTGTTTCGCG
CGGTTCACGATGGCCGCGTGAAGTTCGTTTGGGTCATGGCGACGAACCCGGCGGTGTCGATGCCCGACGCGGGCTTCGTG
CGCGAAGCGCTGGCGCGGTGTGAAACGCTGGTCGTCTCCGACGTGATCGCGGATACGGACACGGCGCGCCTCGCGCATAT
CCGCCTGCCCGCGCTGGCGTGGGGCGAAAAGGACGGCACCGTCACCAATTCCGAGCGGCGAATCAGCCGCCAGCGCGCGC
TGTTCGAGGCACCCGGCCAAGCGCGGGCCGACTGGGCGATCCTGTGCGATTTCGCCGAGCGGATGGGTTGGGGCCGGGCC
TTCGCCTTTCCGACCGCTGCCGCGATATTCCGCGAATACGCGGCGATGACCGCCCTTGCCGGCGCGCGGGGCAAGGCGCT
CGACCTGTCGCATCTGGCGGGGCTTGGCGACGCTGCCTACGAAGCACTGGAGCCGACGCTGTGGGGCGGGGTGCATCCGA
CGGCCCGGACGTTCAGCCACCCTGACGGCAAGGCCCGGCTGGTGGCGGTCGAAGCGCCGGTCGCGGCTCCTGCTGATCCT
GAGTTCCCGTTCCGCCTCAACACCGGGCGTTATCGTGACCAGTGGCACACGATGACGCGCACCGGGCTTTCCCCGACGCT
GTCGCAGCACCGGCGCGAACCCCTGCTCGAAATCCATCCGGAAGACGCGGCGCTTGCAGGGATCGAGGATGGCGGGCTGG
CGAGGCTGGCGACGCGCGTGGGCGCGACGGTTTTCCGAGTCCTGCATTCGACAGCCCAGCGGCGCGGCGATCTGTTCGTG
CCGATGCACTGGACCGATGCGATGGCTGGCGAAGGCCGCGCCAACCGCCTGCCGCTTCAGGAAACCGATCCGCTTTCAGG
CCAGCCCGGGTTCAAGGACACGCCCGCGCGCGTGGAACCGGTCGTGCCGGAATGGCGCGCGTTCCTGGCGACACGTGATG
AGGCCGCGCTTCCATCGCTTTTGTGGTGGAGCCGCGCCAAGGTCGCCAATGGATGGCTCTGCGAACTGGCAGGCAACGGC
GCGCCGGATCTGGAAGTGATACTTCCGAAGGGTACGTGGCTTGAAGCGGCGGACATGGTGCGCGGCATGCGCCGGATCAT
CGTGCTGGATAAGGCGGGCGGGCTTCTGGCCGCGCTTTATCTGACGCGCAACGGCGAACTTCCGCCCCGGGACTGGGTAG
CGGCGCAGATCGGCAACGCCGAGGCATCGGCGCTCGAACTTCTGGCTGGGCGTCCGGCAGTTGCCTTGCCCGATCGCGGG
CCCATCGTGTGCGTCTGCCACGGCGTCGGCGTGAACGAGATTGCCGCTGCCGCCTGCGCTGGCGCGATGACGCTCGAAGC
GATCGCGGGATGCACCCGCGCCGGATCGAATTGCGGAAGCTGCCGCCCGGCCATCGCCCGGATGCTCGACGAGATGAACC
TGGATACACAGGAGGCTGCGGAATGA

Upstream 100 bases:

>100_bases
CGATGGGCGATGACGAGGGCTGCGTGCCCACGATCCCGCTAAAAGTGGACGCGGGCCGCATCTACCTGCTGCGCGAGGCG
GTTCTGGGGAAGAAGGCGGC

Downstream 100 bases:

>100_bases
ACGACTTTCCGGCGGGAATGGTCTGGCTGGTCGGTGCGGGACCCGGCGATCCCGAACTGCTGACGCGCAAGGCCGAACGG
CTGATCGGCAGAGCCAGCGT

Product: assimilatory nitrate reductase (NADH) subunit alpha apoprotein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 861; Mature: 860

Protein sequence:

>861_residues
MTMKDIRTTCAYCGVGCGIRATVTGDRAVSIKGDPEHPANFGRLCSKGTHLGETVGLEGRLLHPMVDAERATWNEALDLA
AARMRECIERHGPDSVAFYVSGQLLTEDYYVANKLMKGFIGSGNIDTNSRLCMASAVAAHNRAFGEDLVPCTYDDLEAAD
LVLLVGSNTAWCHPVIWQRIEAARSARGTRLVVIDPRRTETAEQADLHVALAPDGDIALFNALLAEMRDRDLLDEAFMAE
RCAVPEGYVAGLDPRGHGIDPATFRALADLVAASPRMVTLFSQGANQSRCGTDKGNAIINLHLALGRISQTGAGPFSITG
QPNAMGGREVGGLANMLACHLGFSSEERADAAIFWNAPNICAGPGLKAVDLFRAVHDGRVKFVWVMATNPAVSMPDAGFV
REALARCETLVVSDVIADTDTARLAHIRLPALAWGEKDGTVTNSERRISRQRALFEAPGQARADWAILCDFAERMGWGRA
FAFPTAAAIFREYAAMTALAGARGKALDLSHLAGLGDAAYEALEPTLWGGVHPTARTFSHPDGKARLVAVEAPVAAPADP
EFPFRLNTGRYRDQWHTMTRTGLSPTLSQHRREPLLEIHPEDAALAGIEDGGLARLATRVGATVFRVLHSTAQRRGDLFV
PMHWTDAMAGEGRANRLPLQETDPLSGQPGFKDTPARVEPVVPEWRAFLATRDEAALPSLLWWSRAKVANGWLCELAGNG
APDLEVILPKGTWLEAADMVRGMRRIIVLDKAGGLLAALYLTRNGELPPRDWVAAQIGNAEASALELLAGRPAVALPDRG
PIVCVCHGVGVNEIAAAACAGAMTLEAIAGCTRAGSNCGSCRPAIARMLDEMNLDTQEAAE

Sequences:

>Translated_861_residues
MTMKDIRTTCAYCGVGCGIRATVTGDRAVSIKGDPEHPANFGRLCSKGTHLGETVGLEGRLLHPMVDAERATWNEALDLA
AARMRECIERHGPDSVAFYVSGQLLTEDYYVANKLMKGFIGSGNIDTNSRLCMASAVAAHNRAFGEDLVPCTYDDLEAAD
LVLLVGSNTAWCHPVIWQRIEAARSARGTRLVVIDPRRTETAEQADLHVALAPDGDIALFNALLAEMRDRDLLDEAFMAE
RCAVPEGYVAGLDPRGHGIDPATFRALADLVAASPRMVTLFSQGANQSRCGTDKGNAIINLHLALGRISQTGAGPFSITG
QPNAMGGREVGGLANMLACHLGFSSEERADAAIFWNAPNICAGPGLKAVDLFRAVHDGRVKFVWVMATNPAVSMPDAGFV
REALARCETLVVSDVIADTDTARLAHIRLPALAWGEKDGTVTNSERRISRQRALFEAPGQARADWAILCDFAERMGWGRA
FAFPTAAAIFREYAAMTALAGARGKALDLSHLAGLGDAAYEALEPTLWGGVHPTARTFSHPDGKARLVAVEAPVAAPADP
EFPFRLNTGRYRDQWHTMTRTGLSPTLSQHRREPLLEIHPEDAALAGIEDGGLARLATRVGATVFRVLHSTAQRRGDLFV
PMHWTDAMAGEGRANRLPLQETDPLSGQPGFKDTPARVEPVVPEWRAFLATRDEAALPSLLWWSRAKVANGWLCELAGNG
APDLEVILPKGTWLEAADMVRGMRRIIVLDKAGGLLAALYLTRNGELPPRDWVAAQIGNAEASALELLAGRPAVALPDRG
PIVCVCHGVGVNEIAAAACAGAMTLEAIAGCTRAGSNCGSCRPAIARMLDEMNLDTQEAAE
>Mature_860_residues
TMKDIRTTCAYCGVGCGIRATVTGDRAVSIKGDPEHPANFGRLCSKGTHLGETVGLEGRLLHPMVDAERATWNEALDLAA
ARMRECIERHGPDSVAFYVSGQLLTEDYYVANKLMKGFIGSGNIDTNSRLCMASAVAAHNRAFGEDLVPCTYDDLEAADL
VLLVGSNTAWCHPVIWQRIEAARSARGTRLVVIDPRRTETAEQADLHVALAPDGDIALFNALLAEMRDRDLLDEAFMAER
CAVPEGYVAGLDPRGHGIDPATFRALADLVAASPRMVTLFSQGANQSRCGTDKGNAIINLHLALGRISQTGAGPFSITGQ
PNAMGGREVGGLANMLACHLGFSSEERADAAIFWNAPNICAGPGLKAVDLFRAVHDGRVKFVWVMATNPAVSMPDAGFVR
EALARCETLVVSDVIADTDTARLAHIRLPALAWGEKDGTVTNSERRISRQRALFEAPGQARADWAILCDFAERMGWGRAF
AFPTAAAIFREYAAMTALAGARGKALDLSHLAGLGDAAYEALEPTLWGGVHPTARTFSHPDGKARLVAVEAPVAAPADPE
FPFRLNTGRYRDQWHTMTRTGLSPTLSQHRREPLLEIHPEDAALAGIEDGGLARLATRVGATVFRVLHSTAQRRGDLFVP
MHWTDAMAGEGRANRLPLQETDPLSGQPGFKDTPARVEPVVPEWRAFLATRDEAALPSLLWWSRAKVANGWLCELAGNGA
PDLEVILPKGTWLEAADMVRGMRRIIVLDKAGGLLAALYLTRNGELPPRDWVAAQIGNAEASALELLAGRPAVALPDRGP
IVCVCHGVGVNEIAAAACAGAMTLEAIAGCTRAGSNCGSCRPAIARMLDEMNLDTQEAAE

Specific function: Nitrate reductase is a key enzyme involved in the first step of nitrate assimilation in plants, fungi and bacteria [H]

COG id: COG0243

COG function: function code C; Anaerobic dehydrogenases, typically selenocysteine-containing

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/napA/narB subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788534, Length=791, Percent_Identity=28.9506953223767, Blast_Score=281, Evalue=1e-76,
Organism=Escherichia coli, GI3868721, Length=729, Percent_Identity=27.1604938271605, Blast_Score=240, Evalue=3e-64,
Organism=Escherichia coli, GI3868719, Length=611, Percent_Identity=23.0769230769231, Blast_Score=139, Evalue=7e-34,
Organism=Escherichia coli, GI3868720, Length=621, Percent_Identity=22.3832528180354, Blast_Score=134, Evalue=3e-32,
Organism=Escherichia coli, GI1787778, Length=696, Percent_Identity=23.4195402298851, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI1787870, Length=253, Percent_Identity=24.5059288537549, Blast_Score=75, Evalue=3e-14,
Organism=Escherichia coli, GI87081797, Length=253, Percent_Identity=25.6916996047431, Blast_Score=72, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009010
- InterPro:   IPR007419
- InterPro:   IPR006657
- InterPro:   IPR006656
- InterPro:   IPR006963
- InterPro:   IPR006655 [H]

Pfam domain/function: PF04324 Fer2_BFD; PF04879 Molybdop_Fe4S4; PF00384 Molybdopterin; PF01568 Molydop_binding [H]

EC number: =1.7.99.4 [H]

Molecular weight: Translated: 92202; Mature: 92071

Theoretical pI: Translated: 5.99; Mature: 5.99

Prosite motif: PS00551 MOLYBDOPTERIN_PROK_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTMKDIRTTCAYCGVGCGIRATVTGDRAVSIKGDPEHPANFGRLCSKGTHLGETVGLEGR
CCHHHHHHHHHHHCCCCCEEEEECCCEEEEECCCCCCCHHHHHHHHCCCCCCHHCCCCCE
LLHPMVDAERATWNEALDLAAARMRECIERHGPDSVAFYVSGQLLTEDYYVANKLMKGFI
EECCHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECEEECHHHHHHHHHHHHHC
GSGNIDTNSRLCMASAVAAHNRAFGEDLVPCTYDDLEAADLVLLVGSNTAWCHPVIWQRI
CCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCCCEECHHHHHHH
EAARSARGTRLVVIDPRRTETAEQADLHVALAPDGDIALFNALLAEMRDRDLLDEAFMAE
HHHHHCCCCEEEEECCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHH
RCAVPEGYVAGLDPRGHGIDPATFRALADLVAASPRMVTLFSQGANQSRCGTDKGNAIIN
HHCCCCCCEECCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCEEEE
LHLALGRISQTGAGPFSITGQPNAMGGREVGGLANMLACHLGFSSEERADAAIFWNAPNI
EEEEECCHHCCCCCCEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCC
CAGPGLKAVDLFRAVHDGRVKFVWVMATNPAVSMPDAGFVREALARCETLVVSDVIADTD
CCCCCCHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCC
TARLAHIRLPALAWGEKDGTVTNSERRISRQRALFEAPGQARADWAILCDFAERMGWGRA
CCEEEEEECCCEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCE
FAFPTAAAIFREYAAMTALAGARGKALDLSHLAGLGDAAYEALEPTLWGGVHPTARTFSH
EHHHHHHHHHHHHHHHHHHHCCCCCEECHHHHHCCCHHHHHHHCCHHCCCCCCCHHCCCC
PDGKARLVAVEAPVAAPADPEFPFRLNTGRYRDQWHTMTRTGLSPTLSQHRREPLLEIHP
CCCCEEEEEEECCCCCCCCCCCCEEECCCCCHHHHHHHHHCCCCHHHHHHHCCCCEEECC
EDAALAGIEDGGLARLATRVGATVFRVLHSTAQRRGDLFVPMHWTDAMAGEGRANRLPLQ
CCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCC
ETDPLSGQPGFKDTPARVEPVVPEWRAFLATRDEAALPSLLWWSRAKVANGWLCELAGNG
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCHHCCHHHHHHHHHHCCCEEEEECCCC
APDLEVILPKGTWLEAADMVRGMRRIIVLDKAGGLLAALYLTRNGELPPRDWVAAQIGNA
CCCEEEEECCCCCHHHHHHHHHHEEEEEEECCCCEEEEEEEECCCCCCCHHHHHHHCCCC
EASALELLAGRPAVALPDRGPIVCVCHGVGVNEIAAAACAGAMTLEAIAGCTRAGSNCGS
HHHHHHHHCCCCCEECCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
CRPAIARMLDEMNLDTQEAAE
CHHHHHHHHHHCCCCHHHHCC
>Mature Secondary Structure 
TMKDIRTTCAYCGVGCGIRATVTGDRAVSIKGDPEHPANFGRLCSKGTHLGETVGLEGR
CHHHHHHHHHHHCCCCCEEEEECCCEEEEECCCCCCCHHHHHHHHCCCCCCHHCCCCCE
LLHPMVDAERATWNEALDLAAARMRECIERHGPDSVAFYVSGQLLTEDYYVANKLMKGFI
EECCHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECEEECHHHHHHHHHHHHHC
GSGNIDTNSRLCMASAVAAHNRAFGEDLVPCTYDDLEAADLVLLVGSNTAWCHPVIWQRI
CCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCCCEECHHHHHHH
EAARSARGTRLVVIDPRRTETAEQADLHVALAPDGDIALFNALLAEMRDRDLLDEAFMAE
HHHHHCCCCEEEEECCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHH
RCAVPEGYVAGLDPRGHGIDPATFRALADLVAASPRMVTLFSQGANQSRCGTDKGNAIIN
HHCCCCCCEECCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCEEEE
LHLALGRISQTGAGPFSITGQPNAMGGREVGGLANMLACHLGFSSEERADAAIFWNAPNI
EEEEECCHHCCCCCCEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCC
CAGPGLKAVDLFRAVHDGRVKFVWVMATNPAVSMPDAGFVREALARCETLVVSDVIADTD
CCCCCCHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCC
TARLAHIRLPALAWGEKDGTVTNSERRISRQRALFEAPGQARADWAILCDFAERMGWGRA
CCEEEEEECCCEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCE
FAFPTAAAIFREYAAMTALAGARGKALDLSHLAGLGDAAYEALEPTLWGGVHPTARTFSH
EHHHHHHHHHHHHHHHHHHHCCCCCEECHHHHHCCCHHHHHHHCCHHCCCCCCCHHCCCC
PDGKARLVAVEAPVAAPADPEFPFRLNTGRYRDQWHTMTRTGLSPTLSQHRREPLLEIHP
CCCCEEEEEEECCCCCCCCCCCCEEECCCCCHHHHHHHHHCCCCHHHHHHHCCCCEEECC
EDAALAGIEDGGLARLATRVGATVFRVLHSTAQRRGDLFVPMHWTDAMAGEGRANRLPLQ
CCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCC
ETDPLSGQPGFKDTPARVEPVVPEWRAFLATRDEAALPSLLWWSRAKVANGWLCELAGNG
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCHHCCHHHHHHHHHHCCCEEEEECCCC
APDLEVILPKGTWLEAADMVRGMRRIIVLDKAGGLLAALYLTRNGELPPRDWVAAQIGNA
CCCEEEEECCCCCHHHHHHHHHHEEEEEEECCCCEEEEEEEECCCCCCCHHHHHHHCCCC
EASALELLAGRPAVALPDRGPIVCVCHGVGVNEIAAAACAGAMTLEAIAGCTRAGSNCGS
HHHHHHHHCCCCCEECCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
CRPAIARMLDEMNLDTQEAAE
CHHHHHHHHHHCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8468296 [H]