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The map label for this gene is ysgA [H]

Identifier: 87200913

GI number: 87200913

Start: 3119359

End: 3120162

Strand: Reverse

Name: ysgA [H]

Synonym: Saro_2901

Alternate gene names: 87200913

Gene position: 3120162-3119359 (Counterclockwise)

Preceding gene: 87200914

Following gene: 87200912

Centisome position: 87.61

GC content: 66.29

Gene sequence:

>804_bases
ATGACCCGCAAGATCATCACCGGCTATTCCAACCCGACGGTAAAGTTCGTCCGCAGCCTGCGCGAGAAGAAGCATCGGCG
GCGCGAGCGCAAGTTCCTTGCCGAGGGACTGCGCCTGCTGACGGATGCACGTGAAGGCGGGCGGCTGCCGGAAATCCTCC
TGATGGCCGAAGGGCGCGAAGCCCATCCGCTGCTGGACGAACTGGAACAGGCGGTGGACGCAGCCGGCGGCGAGGTGATC
GAACTGCCGCTGGATATCCTGTCCAAGGTCACCGGCAAGGAAAACCCGCAGGCCGTCGCCGGGGTCTTCGCGGAATGGGA
CACATCTATCAGGCTGATAGATCGCAACGCCGCGCCGATCTGGCTGGTCGCCCATGCGATGCGCGATCCCGGGAACCTCG
GCACCATGCTGCGCACCGCCGACGCGGTGGGCGCGGGCGGGCTCATCCTGATCGACGATTGCGTCGACCCCTTCTCGGTC
GAGGCGGTGCGCGCCAGCATGGGTGCGATCTTTACCGAAAAGCTTGCCCAGGCGCGGTGGGACGAGTTCATCGCCTGGCT
GCGCGCGGGTGAGGGGCAGCTGGTTGCCGCTTCCCTGCGCGATGCCGTGCCTTATCGCGGCGCGCCATACGTCGCGCCAT
GCTTCGTCATGGTCGGCAACGAATCGCGGGGCCTGCCCGAAGATTACGAACTGGCTTGCGACCTGCGCGTGACGATGCCG
ATGAAGGGCCGGGCCGACAGCCTGAACGCGGCCGTGGCAGGCGCGGTACTCGCTTACGAAGTGCTTGCCACGCTCGAGCG
ATAG

Upstream 100 bases:

>100_bases
CCTGATGAAGCTCACCGGCCTGATCAAGGACGGCTTCGGGGAAGACTGAAACAGCTTGCGGGCGGTTGCCGCCGCGCGCT
GGCTGCCGCATGGGGGCGCG

Downstream 100 bases:

>100_bases
GATCGGACTGTCCGGTTAATCCCCCGGTCAGTCGCAAGGGCGCATGTCTGTCTCATGCGCAGTGTCCTTGCCCTTATCGT
CGCCAGCGTCACCCTTGGCG

Product: tRNA/rRNA methyltransferase

Products: S-adenosyl-L-homocysteine; tRNATyr Containing 2'-O-methylguanosine [C]

Alternate protein names: NA

Number of amino acids: Translated: 267; Mature: 266

Protein sequence:

>267_residues
MTRKIITGYSNPTVKFVRSLREKKHRRRERKFLAEGLRLLTDAREGGRLPEILLMAEGREAHPLLDELEQAVDAAGGEVI
ELPLDILSKVTGKENPQAVAGVFAEWDTSIRLIDRNAAPIWLVAHAMRDPGNLGTMLRTADAVGAGGLILIDDCVDPFSV
EAVRASMGAIFTEKLAQARWDEFIAWLRAGEGQLVAASLRDAVPYRGAPYVAPCFVMVGNESRGLPEDYELACDLRVTMP
MKGRADSLNAAVAGAVLAYEVLATLER

Sequences:

>Translated_267_residues
MTRKIITGYSNPTVKFVRSLREKKHRRRERKFLAEGLRLLTDAREGGRLPEILLMAEGREAHPLLDELEQAVDAAGGEVI
ELPLDILSKVTGKENPQAVAGVFAEWDTSIRLIDRNAAPIWLVAHAMRDPGNLGTMLRTADAVGAGGLILIDDCVDPFSV
EAVRASMGAIFTEKLAQARWDEFIAWLRAGEGQLVAASLRDAVPYRGAPYVAPCFVMVGNESRGLPEDYELACDLRVTMP
MKGRADSLNAAVAGAVLAYEVLATLER
>Mature_266_residues
TRKIITGYSNPTVKFVRSLREKKHRRRERKFLAEGLRLLTDAREGGRLPEILLMAEGREAHPLLDELEQAVDAAGGEVIE
LPLDILSKVTGKENPQAVAGVFAEWDTSIRLIDRNAAPIWLVAHAMRDPGNLGTMLRTADAVGAGGLILIDDCVDPFSVE
AVRASMGAIFTEKLAQARWDEFIAWLRAGEGQLVAASLRDAVPYRGAPYVAPCFVMVGNESRGLPEDYELACDLRVTMPM
KGRADSLNAAVAGAVLAYEVLATLER

Specific function: Specifically Methylates Guanosine-18 In Various tRNAs. [C]

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Homo sapiens, GI8922534, Length=157, Percent_Identity=29.9363057324841, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24666840, Length=277, Percent_Identity=23.826714801444, Blast_Score=71, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001537
- InterPro:   IPR013123 [H]

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind [H]

EC number: 2.1.1.34 [C]

Molecular weight: Translated: 29152; Mature: 29021

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRKIITGYSNPTVKFVRSLREKKHRRRERKFLAEGLRLLTDAREGGRLPEILLMAEGRE
CCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCC
AHPLLDELEQAVDAAGGEVIELPLDILSKVTGKENPQAVAGVFAEWDTSIRLIDRNAAPI
CCHHHHHHHHHHHHCCCCEEEECHHHHHHHCCCCCCHHHHHHHHHCCCEEEEEECCCCCE
WLVAHAMRDPGNLGTMLRTADAVGAGGLILIDDCVDPFSVEAVRASMGAIFTEKLAQARW
EEEHHHHCCCCCHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHH
DEFIAWLRAGEGQLVAASLRDAVPYRGAPYVAPCFVMVGNESRGLPEDYELACDLRVTMP
HHHHHHHHCCCCCEEEEHHHHCCCCCCCCCCCEEEEEECCCCCCCCCCCEEEEEEEEECC
MKGRADSLNAAVAGAVLAYEVLATLER
CCCCCCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TRKIITGYSNPTVKFVRSLREKKHRRRERKFLAEGLRLLTDAREGGRLPEILLMAEGRE
CCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCC
AHPLLDELEQAVDAAGGEVIELPLDILSKVTGKENPQAVAGVFAEWDTSIRLIDRNAAPI
CCHHHHHHHHHHHHCCCCEEEECHHHHHHHCCCCCCHHHHHHHHHCCCEEEEEECCCCCE
WLVAHAMRDPGNLGTMLRTADAVGAGGLILIDDCVDPFSVEAVRASMGAIFTEKLAQARW
EEEHHHHCCCCCHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHH
DEFIAWLRAGEGQLVAASLRDAVPYRGAPYVAPCFVMVGNESRGLPEDYELACDLRVTMP
HHHHHHHHCCCCCEEEEHHHHCCCCCCCCCCCEEEEEECCCCCCCCCCCEEEEEEEEECC
MKGRADSLNAAVAGAVLAYEVLATLER
CCCCCCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-Adenosyl-L-methionine; tRNATyr [C]

Specific reaction: S-Adenosyl-L-methionine + tRNATyr = S-adenosyl-L-homocysteine + tRNATyr Containing 2'-O-methylguanosine [C]

General reaction: Methyl group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377 [H]