Click here to switch to the map view.

The map label for this gene is ligF [H]

Identifier: 87200886

GI number: 87200886

Start: 3096844

End: 3097614

Strand: Reverse

Name: ligF [H]

Synonym: Saro_2873

Alternate gene names: 87200886

Gene position: 3097614-3096844 (Counterclockwise)

Preceding gene: 87200887

Following gene: 87200885

Centisome position: 86.97

GC content: 62.0

Gene sequence:

>771_bases
ATGGACGAGGTAAGCCTCTATCATTGGGAACCCAATGCCAACTCTGGCAAGCCGATGCTCGCGTTGATGGAGAAGGGCGT
GCCCTTTTCCAGCCATTACATCGACATGCTCCAGTTCGATCAGCACAAGCCGGAATACCTTGCGATCAACCCGCAAGGCA
CGATCCCGGCGATGACGCACAATGGCCAGGTGCTGACGGAAAGCACCGCGATCATGGAGTACGTGAACGACCGCTTCGAC
GGGCCGGACCTCATGCCCGCCGACGCGCAGGATCGCTGGCGCGTGCGCTGGTGGATGAAGTTCATGGACCAGTGGCTTGG
CCCCAGTTTCTCGATGATCGGCTGGAGCGTGTTTGTCGGTCCCATGGTCCGCCAGCGCGACCCCGCCGAACTTGCCGCCG
CGATCGACCGTATCCCCTTGCCCGAACGCCGCACCGCGTGGCGCAAGGCGATCAACGGCGACTTCTCGGAAAGCGAGATG
GCCGAAAGCCGCCGCCGCGTGGGGCTGGGCATCGCCAAGCTGGAAGAGGAACTGGGCAAGCGGCCCTATGTCGGTTCGAA
CCAGTACAGCCTGGCCGACATCAACATCTTCAACAGCACCTATTCGCTGCCCATTTCCCAGCCCGATCTGGCGGGCAAGG
ACAGGACGCCGAACATCATGCGCTGGCTCAAGCGTGTCTACACCCGCGAAGCGGTGAAGAAGACCTGGGCCATGGGCAAG
ACGGACCTTGCCCATCGCTACGGCCTCATCATGGCGGAGATCGAGGGATGA

Upstream 100 bases:

>100_bases
CCGCGCTCCCGCTGAATTAGTATGTGTTACTAACAAATTCCGGCGCGACTCCGTCGCTGACCGGAATCGGGAGAGAGACA
ATGCAGCGGATGGGCAGCGG

Downstream 100 bases:

>100_bases
GCGCGCTTCTTTACCACGGCGAGCCCAACGGCGCGTCGCTCACCGTTCTTGCGGCGCTTGCGGAAACGGGCCTCGATATC
GAGTGTCGCCGCATCGACCT

Product: glutathione S-transferase-like protein

Products: 4-fumarylacetoacetate

Alternate protein names: NA

Number of amino acids: Translated: 256; Mature: 256

Protein sequence:

>256_residues
MDEVSLYHWEPNANSGKPMLALMEKGVPFSSHYIDMLQFDQHKPEYLAINPQGTIPAMTHNGQVLTESTAIMEYVNDRFD
GPDLMPADAQDRWRVRWWMKFMDQWLGPSFSMIGWSVFVGPMVRQRDPAELAAAIDRIPLPERRTAWRKAINGDFSESEM
AESRRRVGLGIAKLEEELGKRPYVGSNQYSLADINIFNSTYSLPISQPDLAGKDRTPNIMRWLKRVYTREAVKKTWAMGK
TDLAHRYGLIMAEIEG

Sequences:

>Translated_256_residues
MDEVSLYHWEPNANSGKPMLALMEKGVPFSSHYIDMLQFDQHKPEYLAINPQGTIPAMTHNGQVLTESTAIMEYVNDRFD
GPDLMPADAQDRWRVRWWMKFMDQWLGPSFSMIGWSVFVGPMVRQRDPAELAAAIDRIPLPERRTAWRKAINGDFSESEM
AESRRRVGLGIAKLEEELGKRPYVGSNQYSLADINIFNSTYSLPISQPDLAGKDRTPNIMRWLKRVYTREAVKKTWAMGK
TDLAHRYGLIMAEIEG
>Mature_256_residues
MDEVSLYHWEPNANSGKPMLALMEKGVPFSSHYIDMLQFDQHKPEYLAINPQGTIPAMTHNGQVLTESTAIMEYVNDRFD
GPDLMPADAQDRWRVRWWMKFMDQWLGPSFSMIGWSVFVGPMVRQRDPAELAAAIDRIPLPERRTAWRKAINGDFSESEM
AESRRRVGLGIAKLEEELGKRPYVGSNQYSLADINIFNSTYSLPISQPDLAGKDRTPNIMRWLKRVYTREAVKKTWAMGK
TDLAHRYGLIMAEIEG

Specific function: Lignin degradation enzyme [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GST N-terminal domain [H]

Homologues:

None

Paralogues:

None

Copy number: 480 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1982 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010987
- InterPro:   IPR004045
- InterPro:   IPR017933
- InterPro:   IPR004046
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00043 GST_C; PF02798 GST_N [H]

EC number: 5.2.1.2

Molecular weight: Translated: 29385; Mature: 29385

Theoretical pI: Translated: 6.69; Mature: 6.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
5.9 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
5.9 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDEVSLYHWEPNANSGKPMLALMEKGVPFSSHYIDMLQFDQHKPEYLAINPQGTIPAMTH
CCCCEEEEECCCCCCCCHHHHHHHCCCCCCHHHHHHHHHCCCCCCEEEECCCCCCCEEEC
NGQVLTESTAIMEYVNDRFDGPDLMPADAQDRWRVRWWMKFMDQWLGPSFSMIGWSVFVG
CCEEEEHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
PMVRQRDPAELAAAIDRIPLPERRTAWRKAINGDFSESEMAESRRRVGLGIAKLEEELGK
HHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHCC
RPYVGSNQYSLADINIFNSTYSLPISQPDLAGKDRTPNIMRWLKRVYTREAVKKTWAMGK
CCCCCCCCEEEEEEEEECCEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCC
TDLAHRYGLIMAEIEG
HHHHHHHCEEEEEECC
>Mature Secondary Structure
MDEVSLYHWEPNANSGKPMLALMEKGVPFSSHYIDMLQFDQHKPEYLAINPQGTIPAMTH
CCCCEEEEECCCCCCCCHHHHHHHCCCCCCHHHHHHHHHCCCCCCEEEECCCCCCCEEEC
NGQVLTESTAIMEYVNDRFDGPDLMPADAQDRWRVRWWMKFMDQWLGPSFSMIGWSVFVG
CCEEEEHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
PMVRQRDPAELAAAIDRIPLPERRTAWRKAINGDFSESEMAESRRRVGLGIAKLEEELGK
HHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHCC
RPYVGSNQYSLADINIFNSTYSLPISQPDLAGKDRTPNIMRWLKRVYTREAVKKTWAMGK
CCCCCCCCEEEEEEEEECCEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCC
TDLAHRYGLIMAEIEG
HHHHHHHCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 4-maleylacetoacetate

Specific reaction: 4-maleylacetoacetate = 4-fumarylacetoacetate

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1744051; 8495726 [H]