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The map label for this gene is 87200822

Identifier: 87200822

GI number: 87200822

Start: 3033028

End: 3034521

Strand: Reverse

Name: 87200822

Synonym: Saro_2809

Alternate gene names: NA

Gene position: 3034521-3033028 (Counterclockwise)

Preceding gene: 87200823

Following gene: 87200821

Centisome position: 85.2

GC content: 65.93

Gene sequence:

>1494_bases
ATGGGCGCTTTTCCGCAGACGATCTATTTCACCGGCGCGAATGCGCCGGTCGGCGAGGAACCGGACCTCCGCGGCCTGAA
GGTGGAAGGCGATCTGCCCGCCGAAGTGCGCGGCAGCTTCTATCGCGCCATCCCCGATCCCGCGTTTCCGCCCAGGTTCG
AGAACGACCACACCCTGTCCGGCGACGGCATGGTCAGCCGCCTTTCGTTCAACGGCGACGGCACGGCGGACTTCATCCAG
AAGTACGTCGAGACGGCGCGCTACAAGGCGGAGAAGGCTGCGGGCAAGGCGCTGTTCGGCAAGTACCGCAATCCGTTCAC
CGACGATCCGGAAGTGCAAGGCGTGGACCGCACCGTGGCGAACACCACGCCGGTCTGGCACGCGGGCCGGATGCTGATGG
CCAAGGAAGACGGTCGGCCCTATCGCGTCGACCCCCGGACCCTGGCGACGATCGGCTCCTACGATTTCGGCGGCGCGCTC
AAGAGCGAGACGATGACCGCGCACGTACGGATCGACGCTGGGACGGGCGAGCTGTTCTTCTATGGCTACGAGGCGGACGG
CCAGGCTTCGACCAAGGTGGCGTACTGCATTGTCGGCCCGGATGGCGAACTGAAGCGCGAGCAGTGGTTCGATGCGCCCT
ATTGCGCGATGATGCACGATTTCACGATCAGCGAGAACTATGCGCTGTTCCCGATCTACCCGACCACGGCGGACCTCGAC
CGGCTGAAGGCGGGCGGGGAGCATTGGCACCACCAGCCGGAGCTGGACTCGTGGCTGGGCGTGATGCCGCGCTATGGCGA
TGTTTCGGAGATCAAGTGGTTCAAGGGCCCCAAGGGGTGCCATTCGTACCACATGATGAATGCGTGGGAGGATGCCGACG
GCATGCTCCACTTCGACGCCTGCCTCAACAATACCAACGCCTTCGCCTTCATCCGCGAACCGTCGGGCATCCACATGGGG
CCGCAGGATATCAAGGGCGCGCTGACACGCTGGACTGTCGATCCCCGGGCCGATGGCGGCGACGTGGTGGAGACTGTCAT
CGGGCCTCCGGGCGATTTCCCGGTGATCCCGGCGAAGTTGCAGGGGCGCCCGTACAAGACCGGCTGGATGCTGAGCATGA
ATCCCGAACTTCAGGGGCCGCCGCTCTTCGCCGGGCCGGTCGGGGTTAGCTTCAACCTGCTGCTGCGACTGGACGGGATG
GACACGCCCGCGCCGCAGGTCACGGGCGCGCTGGCGCTGCCGCCGATGGCGGGTTTCAACGAGCCGGTGCATGTGCCTGC
CGCCGATCCCGCGAAGGACGGCTGGCTGGTATTCCTTGTCGACCAGCAGGTTGGCGACAATCAGTTCGTGCACGAAGCCT
GGGTTGTCGATGCGGGGAACATCGGCGCGGGCGCTGTGGCCAAGGTGCACATCCCGACGCGGCTGCGACCCCAGGTCCAC
GGCTGGTGGGTGCCCCAGGCGCAACTGGACGCGCTTGAAGGCTCCGCAGCGTGA

Upstream 100 bases:

>100_bases
CGTCAGCATCGAAATGCGCGCTACGGCCTACTGGTGGGCTTGATCTCGGGGAGGGAAACGCTACAAGAACGATAACAATT
GATAGGGTGAGGTTCTTGCC

Downstream 100 bases:

>100_bases
GCCGCATCGTCATTACCGGAGCCTCGGGCAACTATGGCCGGGGCGTGACGGACCGCCTGATCGCGCAGGGGCGCGCCGAA
GACCTGATCCTCATCACGCG

Product: carotenoid oxygenase

Products: NA

Alternate protein names: ACO; 8'-apo-beta-carotenal 15,15'-oxygenase; Diox1 [H]

Number of amino acids: Translated: 497; Mature: 496

Protein sequence:

>497_residues
MGAFPQTIYFTGANAPVGEEPDLRGLKVEGDLPAEVRGSFYRAIPDPAFPPRFENDHTLSGDGMVSRLSFNGDGTADFIQ
KYVETARYKAEKAAGKALFGKYRNPFTDDPEVQGVDRTVANTTPVWHAGRMLMAKEDGRPYRVDPRTLATIGSYDFGGAL
KSETMTAHVRIDAGTGELFFYGYEADGQASTKVAYCIVGPDGELKREQWFDAPYCAMMHDFTISENYALFPIYPTTADLD
RLKAGGEHWHHQPELDSWLGVMPRYGDVSEIKWFKGPKGCHSYHMMNAWEDADGMLHFDACLNNTNAFAFIREPSGIHMG
PQDIKGALTRWTVDPRADGGDVVETVIGPPGDFPVIPAKLQGRPYKTGWMLSMNPELQGPPLFAGPVGVSFNLLLRLDGM
DTPAPQVTGALALPPMAGFNEPVHVPAADPAKDGWLVFLVDQQVGDNQFVHEAWVVDAGNIGAGAVAKVHIPTRLRPQVH
GWWVPQAQLDALEGSAA

Sequences:

>Translated_497_residues
MGAFPQTIYFTGANAPVGEEPDLRGLKVEGDLPAEVRGSFYRAIPDPAFPPRFENDHTLSGDGMVSRLSFNGDGTADFIQ
KYVETARYKAEKAAGKALFGKYRNPFTDDPEVQGVDRTVANTTPVWHAGRMLMAKEDGRPYRVDPRTLATIGSYDFGGAL
KSETMTAHVRIDAGTGELFFYGYEADGQASTKVAYCIVGPDGELKREQWFDAPYCAMMHDFTISENYALFPIYPTTADLD
RLKAGGEHWHHQPELDSWLGVMPRYGDVSEIKWFKGPKGCHSYHMMNAWEDADGMLHFDACLNNTNAFAFIREPSGIHMG
PQDIKGALTRWTVDPRADGGDVVETVIGPPGDFPVIPAKLQGRPYKTGWMLSMNPELQGPPLFAGPVGVSFNLLLRLDGM
DTPAPQVTGALALPPMAGFNEPVHVPAADPAKDGWLVFLVDQQVGDNQFVHEAWVVDAGNIGAGAVAKVHIPTRLRPQVH
GWWVPQAQLDALEGSAA
>Mature_496_residues
GAFPQTIYFTGANAPVGEEPDLRGLKVEGDLPAEVRGSFYRAIPDPAFPPRFENDHTLSGDGMVSRLSFNGDGTADFIQK
YVETARYKAEKAAGKALFGKYRNPFTDDPEVQGVDRTVANTTPVWHAGRMLMAKEDGRPYRVDPRTLATIGSYDFGGALK
SETMTAHVRIDAGTGELFFYGYEADGQASTKVAYCIVGPDGELKREQWFDAPYCAMMHDFTISENYALFPIYPTTADLDR
LKAGGEHWHHQPELDSWLGVMPRYGDVSEIKWFKGPKGCHSYHMMNAWEDADGMLHFDACLNNTNAFAFIREPSGIHMGP
QDIKGALTRWTVDPRADGGDVVETVIGPPGDFPVIPAKLQGRPYKTGWMLSMNPELQGPPLFAGPVGVSFNLLLRLDGMD
TPAPQVTGALALPPMAGFNEPVHVPAADPAKDGWLVFLVDQQVGDNQFVHEAWVVDAGNIGAGAVAKVHIPTRLRPQVHG
WWVPQAQLDALEGSAA

Specific function: Cleaves a number of carotenals and carotenols in the all-trans configuration at the 15-15' double bond producing retinal or retinol, respectively. Also shows activity toward lycopenals and the corresponding alcohols. Does not cleave beta- carotene or lyco

COG id: COG3670

COG function: function code Q; Lignostilbene-alpha,beta-dioxygenase and related enzymes

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the carotenoid oxygenase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004294 [H]

Pfam domain/function: PF03055 RPE65 [H]

EC number: =1.14.99.41 [H]

Molecular weight: Translated: 54364; Mature: 54233

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGAFPQTIYFTGANAPVGEEPDLRGLKVEGDLPAEVRGSFYRAIPDPAFPPRFENDHTLS
CCCCCCEEEEECCCCCCCCCCCCCCEEECCCCCHHHCCCEEECCCCCCCCCCCCCCCEEC
GDGMVSRLSFNGDGTADFIQKYVETARYKAEKAAGKALFGKYRNPFTDDPEVQGVDRTVA
CCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHCCCCCCCCCCCCCCCHHHC
NTTPVWHAGRMLMAKEDGRPYRVDPRTLATIGSYDFGGALKSETMTAHVRIDAGTGELFF
CCCCCEECCEEEEEECCCCCEEECCHHEEEECCCCCCCCCCCCEEEEEEEEECCCCEEEE
YGYEADGQASTKVAYCIVGPDGELKREQWFDAPYCAMMHDFTISENYALFPIYPTTADLD
EEEECCCCCCCEEEEEEECCCCCCCHHHHCCCCHHHHEEEEEECCCEEEEEECCCCCCHH
RLKAGGEHWHHQPELDSWLGVMPRYGDVSEIKWFKGPKGCHSYHMMNAWEDADGMLHFDA
HHHCCCHHCCCCCCHHHHHHCCCCCCCHHHEEEECCCCCCCCEECCCCCCCCCCEEEEEH
CLNNTNAFAFIREPSGIHMGPQDIKGALTRWTVDPRADGGDVVETVIGPPGDFPVIPAKL
HHCCCCEEEEEECCCCCCCCHHHHHCCHHEEEECCCCCCCHHHHHHCCCCCCCCEECHHC
QGRPYKTGWMLSMNPELQGPPLFAGPVGVSFNLLLRLDGMDTPAPQVTGALALPPMAGFN
CCCCCCCEEEEEECCCCCCCCEEECCCCCEEEEEEEECCCCCCCCCCCCEEECCCCCCCC
EPVHVPAADPAKDGWLVFLVDQQVGDNQFVHEAWVVDAGNIGAGAVAKVHIPTRLRPQVH
CCEECCCCCCCCCCEEEEEEECCCCCCCEEEEEEEEECCCCCCCCEEEEECCCCCCCCCC
GWWVPQAQLDALEGSAA
EEECCHHHHHCCCCCCC
>Mature Secondary Structure 
GAFPQTIYFTGANAPVGEEPDLRGLKVEGDLPAEVRGSFYRAIPDPAFPPRFENDHTLS
CCCCCEEEEECCCCCCCCCCCCCCEEECCCCCHHHCCCEEECCCCCCCCCCCCCCCEEC
GDGMVSRLSFNGDGTADFIQKYVETARYKAEKAAGKALFGKYRNPFTDDPEVQGVDRTVA
CCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHCCCCCCCCCCCCCCCHHHC
NTTPVWHAGRMLMAKEDGRPYRVDPRTLATIGSYDFGGALKSETMTAHVRIDAGTGELFF
CCCCCEECCEEEEEECCCCCEEECCHHEEEECCCCCCCCCCCCEEEEEEEEECCCCEEEE
YGYEADGQASTKVAYCIVGPDGELKREQWFDAPYCAMMHDFTISENYALFPIYPTTADLD
EEEECCCCCCCEEEEEEECCCCCCCHHHHCCCCHHHHEEEEEECCCEEEEEECCCCCCHH
RLKAGGEHWHHQPELDSWLGVMPRYGDVSEIKWFKGPKGCHSYHMMNAWEDADGMLHFDA
HHHCCCHHCCCCCCHHHHHHCCCCCCCHHHEEEECCCCCCCCEECCCCCCCCCCEEEEEH
CLNNTNAFAFIREPSGIHMGPQDIKGALTRWTVDPRADGGDVVETVIGPPGDFPVIPAKL
HHCCCCEEEEEECCCCCCCCHHHHHCCHHEEEECCCCCCCHHHHHHCCCCCCCCEECHHC
QGRPYKTGWMLSMNPELQGPPLFAGPVGVSFNLLLRLDGMDTPAPQVTGALALPPMAGFN
CCCCCCCEEEEEECCCCCCCCEEECCCCCEEEEEEEECCCCCCCCCCCCEEECCCCCCCC
EPVHVPAADPAKDGWLVFLVDQQVGDNQFVHEAWVVDAGNIGAGAVAKVHIPTRLRPQVH
CCEECCCCCCCCCCEEEEEEECCCCCCCEEEEEEEEECCCCCCCCEEEEECCCCCCCCCC
GWWVPQAQLDALEGSAA
EEECCHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]