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The map label for this gene is hslU

Identifier: 87200647

GI number: 87200647

Start: 2870737

End: 2872038

Strand: Reverse

Name: hslU

Synonym: Saro_2634

Alternate gene names: 87200647

Gene position: 2872038-2870737 (Counterclockwise)

Preceding gene: 87200648

Following gene: 87200646

Centisome position: 80.64

GC content: 64.82

Gene sequence:

>1302_bases
ATGAACGACTCCCTTACCCCCAAGGCCATCGTCCGCGCGCTGGACGAACATATCGTCGGCCAGACCGCCGCGAAGAAGGC
CGTGGCCGTGGCACTGCGCAATCGTTGGCGCCGTCAGCGGCTGTCCGCCGACCTGCGCGACGAGGTTTCCCCCAAGAACA
TCCTGATGATCGGGCCCACCGGCTGCGGCAAGACCGAGATCAGCCGCCGCCTTGCCAAGCTTGCCGATGCGCCGTTCGTG
AAGGTCGAGGCGACGAAGTTCACCGAAGTCGGCTATGTCGGCCGCGACGTTGAGCAGATCGCGCGCGACCTCGTGGAAGA
GGCGATCCGGCTGGAGAAGGAGCGCCGCCGCGACGCGGTGCGGGAAGCCGCCAGCAAGGCGGCGATGGACCGGCTGCTCA
AGGCGCTGGTCGGCGATGGCGCAAGCGAGGCGACGCGGGAAAGCTTCAAGGCGCGGCTTTCGGACGGCTCGATGAACGAC
GTCGAAGTGGAAATCGAGGTCGAGGATGCGCCATCGATGCCGATGGAAATACCGGGCATGGGCGGTGGGATCGGCATGAT
CAACCTCAGCGACATGATGGGCAAGGCTTTCGGCAAGCAGAACCTCAAGCGTCGCAAGATGCGCGTGGTCGATGCCTGGG
ACAAGCTGGTCGACGAGGAAGCCGAAAAGCGCATGGACCAGGACGATGTCGCGCGCGAGGCGATCCGCAACGCCGAGACC
AACGGCATCGTCTTCCTTGACGAGATCGACAAGATCGCAGTTTCCGACGTGCGCGGCGGTTCGGTGAGCCGCGAGGGCGT
GCAGCGCGATCTCCTGCCGCTGATCGAGGGCACGACGGTCGCCACCAAGTACGGCCCGATGAAGACCGACCACGTGCTGT
TCATCGCGAGCGGGGCGTTCCATGTCGCCAAGCCTTCGGACATGCTGCCCGAACTCCAGGGGCGCCTGCCGATCCGGGTC
GAGCTGAATGCGCTGTCGGAAGACGATTTCGTGCGCATCCTGTCGGAAACGCGGGCCAATCTCGTCGAGCAATACCGCGC
GCTGATCGCGACCGAGAACGTTACGCTGGACATCACCCCCGCAGCGATCCGCGCGATTGCCCGCACCGCCGCGCAGGTCA
ACGAAAGCGTCGAGAACATCGGCGCACGGCGCTTGCAGACGGTGATGGAAAAGCTGCTGGAGGAAGTGAGCTTCGACGCC
GAGGATCGCGCGGGCGAGACCGTCATGGTGGACGAGGCCTACGTGGCCGACAAGCTGGCCAACCTTGCCGGCAACGCGGA
TCTTTCGAAGTACATCCTGTGA

Upstream 100 bases:

>100_bases
CTGACCCCGTTTTACCCCGTTCGTGTCGAGCGAAGTCGAGACACGCCAGCGCCAGGGTGTCTCGACTTCGCTCGACACGA
ACGGATCTTGTGAGATTGCC

Downstream 100 bases:

>100_bases
CGGTCAGGCTGGTCCCGGCCACGAATGAGCACTTCGCGCGAATACTGGCGGGGGAGAGGCCGGACGCAGGCGTGGCTCCG
CCTGATACTCCGGTGGCGGA

Product: ATP-dependent protease ATP-binding subunit HslU

Products: NA

Alternate protein names: Unfoldase HslU [H]

Number of amino acids: Translated: 433; Mature: 433

Protein sequence:

>433_residues
MNDSLTPKAIVRALDEHIVGQTAAKKAVAVALRNRWRRQRLSADLRDEVSPKNILMIGPTGCGKTEISRRLAKLADAPFV
KVEATKFTEVGYVGRDVEQIARDLVEEAIRLEKERRRDAVREAASKAAMDRLLKALVGDGASEATRESFKARLSDGSMND
VEVEIEVEDAPSMPMEIPGMGGGIGMINLSDMMGKAFGKQNLKRRKMRVVDAWDKLVDEEAEKRMDQDDVAREAIRNAET
NGIVFLDEIDKIAVSDVRGGSVSREGVQRDLLPLIEGTTVATKYGPMKTDHVLFIASGAFHVAKPSDMLPELQGRLPIRV
ELNALSEDDFVRILSETRANLVEQYRALIATENVTLDITPAAIRAIARTAAQVNESVENIGARRLQTVMEKLLEEVSFDA
EDRAGETVMVDEAYVADKLANLAGNADLSKYIL

Sequences:

>Translated_433_residues
MNDSLTPKAIVRALDEHIVGQTAAKKAVAVALRNRWRRQRLSADLRDEVSPKNILMIGPTGCGKTEISRRLAKLADAPFV
KVEATKFTEVGYVGRDVEQIARDLVEEAIRLEKERRRDAVREAASKAAMDRLLKALVGDGASEATRESFKARLSDGSMND
VEVEIEVEDAPSMPMEIPGMGGGIGMINLSDMMGKAFGKQNLKRRKMRVVDAWDKLVDEEAEKRMDQDDVAREAIRNAET
NGIVFLDEIDKIAVSDVRGGSVSREGVQRDLLPLIEGTTVATKYGPMKTDHVLFIASGAFHVAKPSDMLPELQGRLPIRV
ELNALSEDDFVRILSETRANLVEQYRALIATENVTLDITPAAIRAIARTAAQVNESVENIGARRLQTVMEKLLEEVSFDA
EDRAGETVMVDEAYVADKLANLAGNADLSKYIL
>Mature_433_residues
MNDSLTPKAIVRALDEHIVGQTAAKKAVAVALRNRWRRQRLSADLRDEVSPKNILMIGPTGCGKTEISRRLAKLADAPFV
KVEATKFTEVGYVGRDVEQIARDLVEEAIRLEKERRRDAVREAASKAAMDRLLKALVGDGASEATRESFKARLSDGSMND
VEVEIEVEDAPSMPMEIPGMGGGIGMINLSDMMGKAFGKQNLKRRKMRVVDAWDKLVDEEAEKRMDQDDVAREAIRNAET
NGIVFLDEIDKIAVSDVRGGSVSREGVQRDLLPLIEGTTVATKYGPMKTDHVLFIASGAFHVAKPSDMLPELQGRLPIRV
ELNALSEDDFVRILSETRANLVEQYRALIATENVTLDITPAAIRAIARTAAQVNESVENIGARRLQTVMEKLLEEVSFDA
EDRAGETVMVDEAYVADKLANLAGNADLSKYIL

Specific function: ATPase subunit of a proteasome-like degradation complex; this subunit has chaperone activity. The binding of ATP and its subsequent hydrolysis by HslU are essential for unfolding of protein substrates subsequently hydrolyzed by HslV. HslU recognizes the N

COG id: COG1220

COG function: function code O; ATP-dependent protease HslVU (ClpYQ), ATPase subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ClpX chaperone family. HslU subfamily [H]

Homologues:

Organism=Homo sapiens, GI7242140, Length=111, Percent_Identity=39.6396396396396, Blast_Score=76, Evalue=7e-14,
Organism=Escherichia coli, GI1790366, Length=441, Percent_Identity=52.1541950113379, Blast_Score=444, Evalue=1e-126,
Organism=Escherichia coli, GI1786642, Length=105, Percent_Identity=45.7142857142857, Blast_Score=96, Evalue=6e-21,
Organism=Saccharomyces cerevisiae, GI6319704, Length=142, Percent_Identity=33.8028169014084, Blast_Score=71, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013093
- InterPro:   IPR003959
- InterPro:   IPR019489
- InterPro:   IPR004491 [H]

Pfam domain/function: PF00004 AAA; PF07724 AAA_2; PF10431 ClpB_D2-small [H]

EC number: NA

Molecular weight: Translated: 47670; Mature: 47670

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDSLTPKAIVRALDEHIVGQTAAKKAVAVALRNRWRRQRLSADLRDEVSPKNILMIGPT
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCEEEECCC
GCGKTEISRRLAKLADAPFVKVEATKFTEVGYVGRDVEQIARDLVEEAIRLEKERRRDAV
CCCHHHHHHHHHHHHCCCCEEEECCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
REAASKAAMDRLLKALVGDGASEATRESFKARLSDGSMNDVEVEIEVEDAPSMPMEIPGM
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCCCCCC
GGGIGMINLSDMMGKAFGKQNLKRRKMRVVDAWDKLVDEEAEKRMDQDDVAREAIRNAET
CCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCC
NGIVFLDEIDKIAVSDVRGGSVSREGVQRDLLPLIEGTTVATKYGPMKTDHVLFIASGAF
CCEEEEECCCHHHHHCCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCCEEEEECCCE
HVAKPSDMLPELQGRLPIRVELNALSEDDFVRILSETRANLVEQYRALIATENVTLDITP
EECCCHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECH
AAIRAIARTAAQVNESVENIGARRLQTVMEKLLEEVSFDAEDRAGETVMVDEAYVADKLA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECHHHHHHHHH
NLAGNADLSKYIL
HHCCCCCHHHHCC
>Mature Secondary Structure
MNDSLTPKAIVRALDEHIVGQTAAKKAVAVALRNRWRRQRLSADLRDEVSPKNILMIGPT
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCEEEECCC
GCGKTEISRRLAKLADAPFVKVEATKFTEVGYVGRDVEQIARDLVEEAIRLEKERRRDAV
CCCHHHHHHHHHHHHCCCCEEEECCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
REAASKAAMDRLLKALVGDGASEATRESFKARLSDGSMNDVEVEIEVEDAPSMPMEIPGM
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCCCCCC
GGGIGMINLSDMMGKAFGKQNLKRRKMRVVDAWDKLVDEEAEKRMDQDDVAREAIRNAET
CCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCC
NGIVFLDEIDKIAVSDVRGGSVSREGVQRDLLPLIEGTTVATKYGPMKTDHVLFIASGAF
CCEEEEECCCHHHHHCCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCCEEEEECCCE
HVAKPSDMLPELQGRLPIRVELNALSEDDFVRILSETRANLVEQYRALIATENVTLDITP
EECCCHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECH
AAIRAIARTAAQVNESVENIGARRLQTVMEKLLEEVSFDAEDRAGETVMVDEAYVADKLA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECHHHHHHHHH
NLAGNADLSKYIL
HHCCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA