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The map label for this gene is exoT [H]

Identifier: 87200642

GI number: 87200642

Start: 2865605

End: 2867119

Strand: Direct

Name: exoT [H]

Synonym: Saro_2629

Alternate gene names: 87200642

Gene position: 2865605-2867119 (Clockwise)

Preceding gene: 87200641

Following gene: 87200643

Centisome position: 80.46

GC content: 66.01

Gene sequence:

>1515_bases
GTGAAGCGAACGCCGAACCCGCCAACAGGGAACACGGCTCCCGCCATGTCGGTCAGGGCGGCGGCCCTGTGGGCGCTTGT
GTCTCAATACCTTTCGTTCGCGATGCAGTTCGCGACGAGCATCGTGCTGGCCAGATGGTTCATCACGCCGGCACAACTCG
GACAATTCTCGATCGCCTTTGCCGCGGTCACGCTGGTGGCTTTCCTGCAGGACTTCGGCGTAACCCGCTACGTCAACGGC
GAACGGGACCTTACGCCCGAGAAGCTGCGGATGGCGTTCACCATATCGGTGGCATTCGCCTGGAGTATAGCTCTGCTCGC
GCTTGCGTCCGCATGGCCGATCGCGGCATTCTATGCGGACCCGGCCTTGTTCGGCATCACGCTGATCGTGGCGTCGTCCT
ATCTACTTGTCCCGCTGGCCATCGTCCCGCAGGCGACTTGCCAGCGGCGCATGGACTATCGCTCCAATGCGATGATCGAG
ATCGGTTCGTCGGTCGCCAACGCGGCAACGGCACTGGTCCTCGCGCTTCTTGGATACGGAGCTCTCGCCCTGGCCTGGGG
CGCGTTCGCCCAGCAGGCAGCCCGGCTCGTCATCAGCCAATGGCGCTCGGGTGGCATGCTTCCCTGGCCGCTTCGCGTAA
GCGAGGCCAGGGCAGTCCTCGCGCTGGGAGGGACCAACACCGTACAGGTGGTGTGCCAGACCGTATCCTCACGCGCGCCC
GAACTTGCCTTGGGCGCGGTGGTCGGCAACGCCGCCGTTGGACTTTTCTCGCGCGCGACAGGCCTGGCCCTCCAGTTGCG
GACGCTGATTGCGGGGGCGGTGACAGGCGTATTCTACCCGGCGTTCCGGCGCCTTCGCGACAGCGGCGAGCCGCTCGGCC
CACCCTATCTGCGCGTAGTCGCCGCCTATACCGGCGTCACCTGGCCGGCCATGGCGGGGATCGCGGTGCTGGCCGATCCG
GTCGTGCGCCTGCTCTATGGCGAGCGCTGGATGGAAGCAGCCCCATTGCTTGCCTGGGTGGCGTTTGCGCAGATGTTCCA
GGTCGCGGTTCCGCTCAATGCAGATCTTCCCCTGCTGCTCGGCCGGCACCGGACGCTCATGCGGGTTATGGTTCTTGAAA
CCATTGCGTCCATCGCGCTCCTCGCCGTCACCGCTCCCTTCGGACTGCTCTGGGTCGCAATCTCGCGCGCCATCCACGGC
ATAGTCTGGGTTGCGATGTACGCGCCCCTGTTATGCAGGTCGGTAGGCCTTGGCGCGCGCCCGCTGATCGCGACCTGGAT
GAAAGGCGCGGTTGCGACAGTCGCAGCCATCGTCCCGGCACTCGCCGGTTTCGTGTTCTGGGCGCCTCCCGGGGAGGCCG
GCTTCCTGCAGGTCCTTGCAACGGCCTGCGCGGGCGTGGCTCTGTGGTTTGTCGCGCTGGTCGTGACTGCCCATCCATTG
CATCGCGAACTGGTTGAACTGGCCGCCAGCACGTTGGCCAGCCGCCGAACCGGCGGGCAATTGCCCGCACGCTGA

Upstream 100 bases:

>100_bases
TGGCGAGGCCGATGCTGCGTTGGCGGAGACGGGCGCACAGTCGCGGCGGTTCAGGCGTGGGCGGATCGGCCATGTTCACC
AGCTTCGTGGAGATCGAGGG

Downstream 100 bases:

>100_bases
ATCGCTGGCCGAACGTCGCGCAGAGGCGTATCATCTCCTGCGGGAGAATGGTCATGCCGCACAGACTCGCAAAGTCGGCG
CTGACAGCCTTGCTGGGCAT

Product: polysaccharide biosynthesis protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 504; Mature: 504

Protein sequence:

>504_residues
MKRTPNPPTGNTAPAMSVRAAALWALVSQYLSFAMQFATSIVLARWFITPAQLGQFSIAFAAVTLVAFLQDFGVTRYVNG
ERDLTPEKLRMAFTISVAFAWSIALLALASAWPIAAFYADPALFGITLIVASSYLLVPLAIVPQATCQRRMDYRSNAMIE
IGSSVANAATALVLALLGYGALALAWGAFAQQAARLVISQWRSGGMLPWPLRVSEARAVLALGGTNTVQVVCQTVSSRAP
ELALGAVVGNAAVGLFSRATGLALQLRTLIAGAVTGVFYPAFRRLRDSGEPLGPPYLRVVAAYTGVTWPAMAGIAVLADP
VVRLLYGERWMEAAPLLAWVAFAQMFQVAVPLNADLPLLLGRHRTLMRVMVLETIASIALLAVTAPFGLLWVAISRAIHG
IVWVAMYAPLLCRSVGLGARPLIATWMKGAVATVAAIVPALAGFVFWAPPGEAGFLQVLATACAGVALWFVALVVTAHPL
HRELVELAASTLASRRTGGQLPAR

Sequences:

>Translated_504_residues
MKRTPNPPTGNTAPAMSVRAAALWALVSQYLSFAMQFATSIVLARWFITPAQLGQFSIAFAAVTLVAFLQDFGVTRYVNG
ERDLTPEKLRMAFTISVAFAWSIALLALASAWPIAAFYADPALFGITLIVASSYLLVPLAIVPQATCQRRMDYRSNAMIE
IGSSVANAATALVLALLGYGALALAWGAFAQQAARLVISQWRSGGMLPWPLRVSEARAVLALGGTNTVQVVCQTVSSRAP
ELALGAVVGNAAVGLFSRATGLALQLRTLIAGAVTGVFYPAFRRLRDSGEPLGPPYLRVVAAYTGVTWPAMAGIAVLADP
VVRLLYGERWMEAAPLLAWVAFAQMFQVAVPLNADLPLLLGRHRTLMRVMVLETIASIALLAVTAPFGLLWVAISRAIHG
IVWVAMYAPLLCRSVGLGARPLIATWMKGAVATVAAIVPALAGFVFWAPPGEAGFLQVLATACAGVALWFVALVVTAHPL
HRELVELAASTLASRRTGGQLPAR
>Mature_504_residues
MKRTPNPPTGNTAPAMSVRAAALWALVSQYLSFAMQFATSIVLARWFITPAQLGQFSIAFAAVTLVAFLQDFGVTRYVNG
ERDLTPEKLRMAFTISVAFAWSIALLALASAWPIAAFYADPALFGITLIVASSYLLVPLAIVPQATCQRRMDYRSNAMIE
IGSSVANAATALVLALLGYGALALAWGAFAQQAARLVISQWRSGGMLPWPLRVSEARAVLALGGTNTVQVVCQTVSSRAP
ELALGAVVGNAAVGLFSRATGLALQLRTLIAGAVTGVFYPAFRRLRDSGEPLGPPYLRVVAAYTGVTWPAMAGIAVLADP
VVRLLYGERWMEAAPLLAWVAFAQMFQVAVPLNADLPLLLGRHRTLMRVMVLETIASIALLAVTAPFGLLWVAISRAIHG
IVWVAMYAPLLCRSVGLGARPLIATWMKGAVATVAAIVPALAGFVFWAPPGEAGFLQVLATACAGVALWFVALVVTAHPL
HRELVELAASTLASRRTGGQLPAR

Specific function: Lipopolysaccharide biosynthesis. [C]

COG id: COG2244

COG function: function code R; Membrane protein involved in the export of O-antigen and teichoic acid

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polysaccharide synthase family [H]

Homologues:

Organism=Escherichia coli, GI1788359, Length=486, Percent_Identity=20.5761316872428, Blast_Score=74, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002797 [H]

Pfam domain/function: PF01943 Polysacc_synt [H]

EC number: NA

Molecular weight: Translated: 53427; Mature: 53427

Theoretical pI: Translated: 10.76; Mature: 10.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRTPNPPTGNTAPAMSVRAAALWALVSQYLSFAMQFATSIVLARWFITPAQLGQFSIAF
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCHHHHHH
AAVTLVAFLQDFGVTRYVNGERDLTPEKLRMAFTISVAFAWSIALLALASAWPIAAFYAD
HHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHC
PALFGITLIVASSYLLVPLAIVPQATCQRRMDYRSNAMIEIGSSVANAATALVLALLGYG
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCEEECHHHHHHHHHHHHHHHHHH
ALALAWGAFAQQAARLVISQWRSGGMLPWPLRVSEARAVLALGGTNTVQVVCQTVSSRAP
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHCCCEEEEECCCHHHHHHHHHHHCCCC
ELALGAVVGNAAVGLFSRATGLALQLRTLIAGAVTGVFYPAFRRLRDSGEPLGPPYLRVV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHH
AAYTGVTWPAMAGIAVLADPVVRLLYGERWMEAAPLLAWVAFAQMFQVAVPLNADLPLLL
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
GRHRTLMRVMVLETIASIALLAVTAPFGLLWVAISRAIHGIVWVAMYAPLLCRSVGLGAR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
PLIATWMKGAVATVAAIVPALAGFVFWAPPGEAGFLQVLATACAGVALWFVALVVTAHPL
HHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHH
HRELVELAASTLASRRTGGQLPAR
HHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MKRTPNPPTGNTAPAMSVRAAALWALVSQYLSFAMQFATSIVLARWFITPAQLGQFSIAF
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCHHHHHH
AAVTLVAFLQDFGVTRYVNGERDLTPEKLRMAFTISVAFAWSIALLALASAWPIAAFYAD
HHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHC
PALFGITLIVASSYLLVPLAIVPQATCQRRMDYRSNAMIEIGSSVANAATALVLALLGYG
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCEEECHHHHHHHHHHHHHHHHHH
ALALAWGAFAQQAARLVISQWRSGGMLPWPLRVSEARAVLALGGTNTVQVVCQTVSSRAP
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHCCCEEEEECCCHHHHHHHHHHHCCCC
ELALGAVVGNAAVGLFSRATGLALQLRTLIAGAVTGVFYPAFRRLRDSGEPLGPPYLRVV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHH
AAYTGVTWPAMAGIAVLADPVVRLLYGERWMEAAPLLAWVAFAQMFQVAVPLNADLPLLL
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
GRHRTLMRVMVLETIASIALLAVTAPFGLLWVAISRAIHGIVWVAMYAPLLCRSVGLGAR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
PLIATWMKGAVATVAAIVPALAGFVFWAPPGEAGFLQVLATACAGVALWFVALVVTAHPL
HHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHH
HRELVELAASTLASRRTGGQLPAR
HHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226645; 8226646; 8118055; 11481431 [H]