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The map label for this gene is glnA [H]

Identifier: 87200622

GI number: 87200622

Start: 2820902

End: 2822227

Strand: Direct

Name: glnA [H]

Synonym: Saro_2609

Alternate gene names: 87200622

Gene position: 2820902-2822227 (Clockwise)

Preceding gene: 87200621

Following gene: 87200623

Centisome position: 79.2

GC content: 66.14

Gene sequence:

>1326_bases
ATGAACACCATGCCGAAGATCGACGCTCAGTTCGCGCAAGGTCTTGCTGCGGAGCGATGCCGCGTCCTGTTCGCCGACCA
GCTCAACCTCGCGCGCGGCAAGTACGTGCCGATGGCGGATGCCGCGCGGGGGCACACCCGCATGTGCGTGGGGACCTTTG
CCGTGACCTATGACAAGGCGCTGGTCGCCGCTCCCGGCGGGGGGATGCTCGACGGCCTGCCGGACATGGAGGTCACCTTC
GACCCGCAGGCACTGCGGCCGTCCTGGGACCCGGGCACGAAGATCGCGCTTGGCAGCCTGCGCTTCAAGGGCGAACCCTT
CGCGCTGTGCGGGCGCAGCGCGCTGCAACGCGCGATCGACGCCTGGCGCGCGCGCGGGCTGGAGCCGATGGTGGGCATCG
AGATGGAAGCCTATGTCTTCCAGCGCGGCGCCGATGGCGGCTGGGTGCCCTATGACACCCCCGGCGCCTTCGTCTATGGC
ACCGGCCCGTTCTCCGACCCGGCGGGATTGATCGACGAGATCTGGCGCACTGCCGAGGCGTGCGGCATTCCGGTGGAATC
GATGAACGCGGAGTTCGACGCCCCGCAGTTCGAACTGACGCTGCGCTATGCCGACGCGATGAAGGCGGCGGACGATGCCT
TCCTTTTCCGCCAGATGGCGCGCGAAGTGCTGTATAAACGAGGCTACCTGCTGTCGTTCCTGCCCAAGCCATTTGCGCAG
AAAAGCGGCAGCGGCCTGCACTTCAACCTGAGCTTCACCAAGGACGACGGCGGCAACGTCTTTGCCAACGACGTCGCGAC
CGGTACGCTCTCACCCGAAATGAAGGGCTGCATCGCCGGGCTGATCCGCCACCACGAAGCGCTTGCCGCGATCATGGCGC
CGATAACCAACAGCTATGCCCGCCTCCAGCCAGCCAGCCTTTCCGGATACTGGGCGAACTGGGGCATCGACCATCGCTCG
GTAACCGTGCGAGTATCGGCCGAGACAGGCCCAGCAGCACGCATCGAGCACCGCATGGCCGATTGCGCCGCCAGCCCATA
CTTCGCGATGGCAGCCATGCTGCAGGCCGCGCTGCTCGGCCTCGAGAACGGCTACGACCTGCCCGAGCCGGAAACCAACG
ACGGGCTGGAGACTATCAACACCGACCGTCACACACCGCACTGCCTGTCCGACGCTCTGGATGCGCTGGAGGCGGACACG
GCTCTGGCATATGCCGTGGGCGCGGACCTCGTCGCCAACTTCGTCGCGATCAAGCGCGACGAAGTGCGGATCGTCGGCGA
CCTGAGCGAGGCCGAACAACTCGCCTACTACCTGCATTACGTCTGA

Upstream 100 bases:

>100_bases
TTCATTCACGGCATCAAGCACCTGCCGGTTCGCATTGTCGCCCGCTGAACGCAGTCCGCAAAGGACTGCCGGAAAAAGCG
GAAGCGACCTGGGGAGACAC

Downstream 100 bases:

>100_bases
GGTCCCCATGCGCGAACGCATCGACATTGCCGGCATTCCCGTTTCGCCCGATCATTTCATCGGCGGCAAGCGGGTGTCAT
CGCCCTATACCTTCGAAACG

Product: glutamate--ammonia ligase

Products: NA

Alternate protein names: GS; Glutamate--ammonia ligase [H]

Number of amino acids: Translated: 441; Mature: 441

Protein sequence:

>441_residues
MNTMPKIDAQFAQGLAAERCRVLFADQLNLARGKYVPMADAARGHTRMCVGTFAVTYDKALVAAPGGGMLDGLPDMEVTF
DPQALRPSWDPGTKIALGSLRFKGEPFALCGRSALQRAIDAWRARGLEPMVGIEMEAYVFQRGADGGWVPYDTPGAFVYG
TGPFSDPAGLIDEIWRTAEACGIPVESMNAEFDAPQFELTLRYADAMKAADDAFLFRQMAREVLYKRGYLLSFLPKPFAQ
KSGSGLHFNLSFTKDDGGNVFANDVATGTLSPEMKGCIAGLIRHHEALAAIMAPITNSYARLQPASLSGYWANWGIDHRS
VTVRVSAETGPAARIEHRMADCAASPYFAMAAMLQAALLGLENGYDLPEPETNDGLETINTDRHTPHCLSDALDALEADT
ALAYAVGADLVANFVAIKRDEVRIVGDLSEAEQLAYYLHYV

Sequences:

>Translated_441_residues
MNTMPKIDAQFAQGLAAERCRVLFADQLNLARGKYVPMADAARGHTRMCVGTFAVTYDKALVAAPGGGMLDGLPDMEVTF
DPQALRPSWDPGTKIALGSLRFKGEPFALCGRSALQRAIDAWRARGLEPMVGIEMEAYVFQRGADGGWVPYDTPGAFVYG
TGPFSDPAGLIDEIWRTAEACGIPVESMNAEFDAPQFELTLRYADAMKAADDAFLFRQMAREVLYKRGYLLSFLPKPFAQ
KSGSGLHFNLSFTKDDGGNVFANDVATGTLSPEMKGCIAGLIRHHEALAAIMAPITNSYARLQPASLSGYWANWGIDHRS
VTVRVSAETGPAARIEHRMADCAASPYFAMAAMLQAALLGLENGYDLPEPETNDGLETINTDRHTPHCLSDALDALEADT
ALAYAVGADLVANFVAIKRDEVRIVGDLSEAEQLAYYLHYV
>Mature_441_residues
MNTMPKIDAQFAQGLAAERCRVLFADQLNLARGKYVPMADAARGHTRMCVGTFAVTYDKALVAAPGGGMLDGLPDMEVTF
DPQALRPSWDPGTKIALGSLRFKGEPFALCGRSALQRAIDAWRARGLEPMVGIEMEAYVFQRGADGGWVPYDTPGAFVYG
TGPFSDPAGLIDEIWRTAEACGIPVESMNAEFDAPQFELTLRYADAMKAADDAFLFRQMAREVLYKRGYLLSFLPKPFAQ
KSGSGLHFNLSFTKDDGGNVFANDVATGTLSPEMKGCIAGLIRHHEALAAIMAPITNSYARLQPASLSGYWANWGIDHRS
VTVRVSAETGPAARIEHRMADCAASPYFAMAAMLQAALLGLENGYDLPEPETNDGLETINTDRHTPHCLSDALDALEADT
ALAYAVGADLVANFVAIKRDEVRIVGDLSEAEQLAYYLHYV

Specific function: Unknown

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Homo sapiens, GI221307485, Length=342, Percent_Identity=24.5614035087719, Blast_Score=92, Evalue=1e-18,
Organism=Escherichia coli, GI87081870, Length=417, Percent_Identity=30.6954436450839, Blast_Score=159, Evalue=4e-40,
Organism=Escherichia coli, GI1790301, Length=275, Percent_Identity=30.5454545454545, Blast_Score=116, Evalue=3e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146
- InterPro:   IPR004809 [H]

Pfam domain/function: PF00120 Gln-synt_C; PF03951 Gln-synt_N [H]

EC number: =6.3.1.2 [H]

Molecular weight: Translated: 47810; Mature: 47810

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: PS00237 G_PROTEIN_RECEP_F1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTMPKIDAQFAQGLAAERCRVLFADQLNLARGKYVPMADAARGHTRMCVGTFAVTYDKA
CCCCCCCHHHHHHCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEEEEECCE
LVAAPGGGMLDGLPDMEVTFDPQALRPSWDPGTKIALGSLRFKGEPFALCGRSALQRAID
EEECCCCCCCCCCCCCEEEECCHHCCCCCCCCCEEEEEEEEECCCCHHHHHHHHHHHHHH
AWRARGLEPMVGIEMEAYVFQRGADGGWVPYDTPGAFVYGTGPFSDPAGLIDEIWRTAEA
HHHHCCCCCCCCCEEEEHHEEECCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHH
CGIPVESMNAEFDAPQFELTLRYADAMKAADDAFLFRQMAREVLYKRGYLLSFLPKPFAQ
HCCCHHHCCCCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCHHHH
KSGSGLHFNLSFTKDDGGNVFANDVATGTLSPEMKGCIAGLIRHHEALAAIMAPITNSYA
CCCCCEEEEEEEEECCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHH
RLQPASLSGYWANWGIDHRSVTVRVSAETGPAARIEHRMADCAASPYFAMAAMLQAALLG
HCCCCCCCCEEECCCCCCCEEEEEEECCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
LENGYDLPEPETNDGLETINTDRHTPHCLSDALDALEADTALAYAVGADLVANFVAIKRD
CCCCCCCCCCCCCCCCHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
EVRIVGDLSEAEQLAYYLHYV
CEEEEECHHHHHHHHHHHHCC
>Mature Secondary Structure
MNTMPKIDAQFAQGLAAERCRVLFADQLNLARGKYVPMADAARGHTRMCVGTFAVTYDKA
CCCCCCCHHHHHHCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEEEEECCE
LVAAPGGGMLDGLPDMEVTFDPQALRPSWDPGTKIALGSLRFKGEPFALCGRSALQRAID
EEECCCCCCCCCCCCCEEEECCHHCCCCCCCCCEEEEEEEEECCCCHHHHHHHHHHHHHH
AWRARGLEPMVGIEMEAYVFQRGADGGWVPYDTPGAFVYGTGPFSDPAGLIDEIWRTAEA
HHHHCCCCCCCCCEEEEHHEEECCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHH
CGIPVESMNAEFDAPQFELTLRYADAMKAADDAFLFRQMAREVLYKRGYLLSFLPKPFAQ
HCCCHHHCCCCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCHHHH
KSGSGLHFNLSFTKDDGGNVFANDVATGTLSPEMKGCIAGLIRHHEALAAIMAPITNSYA
CCCCCEEEEEEEEECCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHH
RLQPASLSGYWANWGIDHRSVTVRVSAETGPAARIEHRMADCAASPYFAMAAMLQAALLG
HCCCCCCCCEEECCCCCCCEEEEEEECCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
LENGYDLPEPETNDGLETINTDRHTPHCLSDALDALEADTALAYAVGADLVANFVAIKRD
CCCCCCCCCCCCCCCCHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
EVRIVGDLSEAEQLAYYLHYV
CEEEEECHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9371463 [H]