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The map label for this gene is eda [H]

Identifier: 87200581

GI number: 87200581

Start: 2772819

End: 2773433

Strand: Reverse

Name: eda [H]

Synonym: Saro_2568

Alternate gene names: 87200581

Gene position: 2773433-2772819 (Counterclockwise)

Preceding gene: 87200582

Following gene: 87200580

Centisome position: 77.87

GC content: 67.15

Gene sequence:

>615_bases
ATGAAGCCGATCGAAACTATCATGCGCACCGCGCCGGTCATCCCGGTGCTGGTCATCGAGGACGTTGACCACGCCCTCCC
CATCGCCCGTGCGCTCGTGAAGGGCGGGCTCAAGGTGCTCGAAGTCACGCTTCGCACTCCTGCCGCGCTCGACGTGATCC
ACGCCATGAAGGGCGTCGAAGGCGCCATCGTCGGCGCGGGCACGGTCACGAACCCCGATGAACTGAAGGCCGCGATCGAC
GCGGGCGCGGAATTCATCGTCTCGCCGGGCCTTACCGACAATCTCGGCAAGGCGGCGGTCTCCGCAGGCATTCCGTTCCT
GCCGGGCATCGCTTCGGCCGGAGACATCATGCGCGGCCTCGATCTGGGCCTCACGCACTTCAAGTTCTTCCCTGCCTCGA
CGTCCGGCGGCCTGCCGGCGCTCAAGGCGCTCGCGGCGCCGTTCCACCAGTGCCGTTTCTGCCCGACCGGCGGCATTACC
GCCGCCACCGCGCCCGAGTGGCTGGGTTTCGACCCCGTCCTGTGCGTCGGTGGCAGCTGGGTTGTCGGTAAGGGCAAACC
CGATGAAGCAGCAATCGAAGCCGCCGCGCGCGAAGCTTCGGCATTGGGGCAGTAA

Upstream 100 bases:

>100_bases
CTATGCGCCCTGCCTGAGCAGGTGCACAATAAGGCTGAGTCGCGTTAAGCCACCATCCGCAACGGAGTGCGCACCAGTCG
GGTCGCGCAGGGGAGACGCC

Downstream 100 bases:

>100_bases
GCGGATACAGGGAATTCAGTGGACATGAAGACGGTCGCCAAGCTCAAGGCCAGGTTGCAGAAACTCCATGAGCGGACGGC
CGAAACGCCGCTGTTCAACC

Product: 2-keto-3-deoxy-phosphogluconate aldolase

Products: NA

Alternate protein names: 4-hydroxy-2-oxoglutarate aldolase; 2-keto-4-hydroxyglutarate aldolase; KHG-aldolase; 2-dehydro-3-deoxy-phosphogluconate aldolase; 2-keto-3-deoxy-6-phosphogluconate aldolase; KDPG-aldolase; Phospho-2-dehydro-3-deoxygluconate aldolase; Phospho-2-keto-3-deoxygluconate aldolase [H]

Number of amino acids: Translated: 204; Mature: 204

Protein sequence:

>204_residues
MKPIETIMRTAPVIPVLVIEDVDHALPIARALVKGGLKVLEVTLRTPAALDVIHAMKGVEGAIVGAGTVTNPDELKAAID
AGAEFIVSPGLTDNLGKAAVSAGIPFLPGIASAGDIMRGLDLGLTHFKFFPASTSGGLPALKALAAPFHQCRFCPTGGIT
AATAPEWLGFDPVLCVGGSWVVGKGKPDEAAIEAAAREASALGQ

Sequences:

>Translated_204_residues
MKPIETIMRTAPVIPVLVIEDVDHALPIARALVKGGLKVLEVTLRTPAALDVIHAMKGVEGAIVGAGTVTNPDELKAAID
AGAEFIVSPGLTDNLGKAAVSAGIPFLPGIASAGDIMRGLDLGLTHFKFFPASTSGGLPALKALAAPFHQCRFCPTGGIT
AATAPEWLGFDPVLCVGGSWVVGKGKPDEAAIEAAAREASALGQ
>Mature_204_residues
MKPIETIMRTAPVIPVLVIEDVDHALPIARALVKGGLKVLEVTLRTPAALDVIHAMKGVEGAIVGAGTVTNPDELKAAID
AGAEFIVSPGLTDNLGKAAVSAGIPFLPGIASAGDIMRGLDLGLTHFKFFPASTSGGLPALKALAAPFHQCRFCPTGGIT
AATAPEWLGFDPVLCVGGSWVVGKGKPDEAAIEAAAREASALGQ

Specific function: KEY ENZYME IN THE ENTNER-DOUDOROFF PATHWAY. PARTICIPATES IN THE REGULATION OF THE INTRACELLULAR LEVEL OF GLYOXYLATE. [C]

COG id: COG0800

COG function: function code G; 2-keto-3-deoxy-6-phosphogluconate aldolase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the KHG/KDPG aldolase family [H]

Homologues:

Organism=Escherichia coli, GI1788156, Length=179, Percent_Identity=54.7486033519553, Blast_Score=186, Evalue=7e-49,
Organism=Escherichia coli, GI48994954, Length=161, Percent_Identity=31.055900621118, Blast_Score=64, Evalue=7e-12,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000887
- InterPro:   IPR013785 [H]

Pfam domain/function: PF01081 Aldolase [H]

EC number: =4.1.3.16; =4.1.2.14 [H]

Molecular weight: Translated: 20720; Mature: 20720

Theoretical pI: Translated: 5.63; Mature: 5.63

Prosite motif: PS00159 ALDOLASE_KDPG_KHG_1 ; PS00160 ALDOLASE_KDPG_KHG_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPIETIMRTAPVIPVLVIEDVDHALPIARALVKGGLKVLEVTLRTPAALDVIHAMKGVE
CCHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHCCCC
GAIVGAGTVTNPDELKAAIDAGAEFIVSPGLTDNLGKAAVSAGIPFLPGIASAGDIMRGL
CEEEECCCCCCHHHHHHHHHCCCCEEECCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHH
DLGLTHFKFFPASTSGGLPALKALAAPFHQCRFCPTGGITAATAPEWLGFDPVLCVGGSW
HCCCHHEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEECCCE
VVGKGKPDEAAIEAAAREASALGQ
EECCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKPIETIMRTAPVIPVLVIEDVDHALPIARALVKGGLKVLEVTLRTPAALDVIHAMKGVE
CCHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHCCCC
GAIVGAGTVTNPDELKAAIDAGAEFIVSPGLTDNLGKAAVSAGIPFLPGIASAGDIMRGL
CEEEECCCCCCHHHHHHHHHCCCCEEECCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHH
DLGLTHFKFFPASTSGGLPALKALAAPFHQCRFCPTGGITAATAPEWLGFDPVLCVGGSW
HCCCHHEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEECCCE
VVGKGKPDEAAIEAAAREASALGQ
EECCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1809834 [H]