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The map label for this gene is sseA [H]

Identifier: 87200566

GI number: 87200566

Start: 2755872

End: 2756741

Strand: Direct

Name: sseA [H]

Synonym: Saro_2553

Alternate gene names: 87200566

Gene position: 2755872-2756741 (Clockwise)

Preceding gene: 87200565

Following gene: 87200567

Centisome position: 77.38

GC content: 65.98

Gene sequence:

>870_bases
ATGCAGCAGGCAGGAGCACAGGCTAAGATGGATTCGCTGGTTTCGACCCAGTGGCTCGCGAACGAAATGGGCGCGAGCGA
CCTCCGTATCGTCGACGCAACGGCGTTCCTGCCGGAGCACGGCCGCAACGCCCTGCTGGAATACGAGGCCTGCCATATCC
CCGGCGCGGTGTTCATGGACCTTGCGGACCTTGTCGATTCGGCATCCGCCGTGCCGAACACCCTGCCCCCGGCCGAGAAA
TTCGCCAGCAGGATGCAGGCCCTGGGCCTTGGCGACGGCAGCCGCGTCGTGATCTACGACGACAGCCCGATCAAGTCCGC
CACCCGCGCCTGGTTCATGCTGACGATGTTCGGGGCGCAGAACGTGGCGCTGCTCGACGGCGGCATCGCCAAGTGGAAAG
CGGAAGGCCGCAAGTGCGCCCAGGGCCGCGAAACCTTGCGCGCCCGCCACTTCACCGTGTGGTCCGATCAGAGCCACGTG
CGCACCAAGGGCGATGTCCTCGCCAATCTGGACACCAAGGCCGAACAGGTGGTCGACGCGCGGGGCGCGGGCCGCTTTAC
CGGCGAGATGGCGGAAACCAATCCGGCCGTGGCGAGCGGGCACATCCCCGGTGCGCGCAACGTGCCCTATTCCAGCCTGT
TCAACGCCGACGGCACGTGGAAATCGCCCGACGCGATCCGCGCTATCTTCGAGGCGGCGGGAGTCGATCTTTCGCGTCCA
CTGATCTCGTCGTGCGGATCGGGCATGACCGCCAACGTGGTGATCTTCGCCCTGCACCTGATCGGCAAGGACGACGTATC
GCTCTATGACGGATCGTGGAGCGAATGGGGGGTCGATCCCGAAACGCCCAAGGCGACTGGACCGGCGTGA

Upstream 100 bases:

>100_bases
CGTCCCGGGATGATGCGCGCATCGGCCCTTTTACGGCTCGACGGTCCCGAACAGGCGGTCTAGTCTGCCAGGCACGAACC
CGCGCAAAGACGGGTCGCGC

Downstream 100 bases:

>100_bases
GAAACTTCCCCGGGGGCTGAAGCCGCAGGGAAGCAGTCGAAACTGGTGGGCATTGCTGCTAACAGCGGGCCGATGAGCAG
TTCCGACAACATGAGCGATC

Product: 3-mercaptopyruvate sulfurtransferase

Products: NA

Alternate protein names: MST; Rhodanese-like protein [H]

Number of amino acids: Translated: 289; Mature: 289

Protein sequence:

>289_residues
MQQAGAQAKMDSLVSTQWLANEMGASDLRIVDATAFLPEHGRNALLEYEACHIPGAVFMDLADLVDSASAVPNTLPPAEK
FASRMQALGLGDGSRVVIYDDSPIKSATRAWFMLTMFGAQNVALLDGGIAKWKAEGRKCAQGRETLRARHFTVWSDQSHV
RTKGDVLANLDTKAEQVVDARGAGRFTGEMAETNPAVASGHIPGARNVPYSSLFNADGTWKSPDAIRAIFEAAGVDLSRP
LISSCGSGMTANVVIFALHLIGKDDVSLYDGSWSEWGVDPETPKATGPA

Sequences:

>Translated_289_residues
MQQAGAQAKMDSLVSTQWLANEMGASDLRIVDATAFLPEHGRNALLEYEACHIPGAVFMDLADLVDSASAVPNTLPPAEK
FASRMQALGLGDGSRVVIYDDSPIKSATRAWFMLTMFGAQNVALLDGGIAKWKAEGRKCAQGRETLRARHFTVWSDQSHV
RTKGDVLANLDTKAEQVVDARGAGRFTGEMAETNPAVASGHIPGARNVPYSSLFNADGTWKSPDAIRAIFEAAGVDLSRP
LISSCGSGMTANVVIFALHLIGKDDVSLYDGSWSEWGVDPETPKATGPA
>Mature_289_residues
MQQAGAQAKMDSLVSTQWLANEMGASDLRIVDATAFLPEHGRNALLEYEACHIPGAVFMDLADLVDSASAVPNTLPPAEK
FASRMQALGLGDGSRVVIYDDSPIKSATRAWFMLTMFGAQNVALLDGGIAKWKAEGRKCAQGRETLRARHFTVWSDQSHV
RTKGDVLANLDTKAEQVVDARGAGRFTGEMAETNPAVASGHIPGARNVPYSSLFNADGTWKSPDAIRAIFEAAGVDLSRP
LISSCGSGMTANVVIFALHLIGKDDVSLYDGSWSEWGVDPETPKATGPA

Specific function: Transfers a sulfur ion to cyanide or to other thiol compounds. Also has weak rhodanese activity. Its participation in detoxification of cyanide may be small. May be involved in the enhancement of serine sensitivity [H]

COG id: COG2897

COG function: function code P; Rhodanese-related sulfurtransferase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 rhodanese domains [H]

Homologues:

Organism=Homo sapiens, GI194473668, Length=271, Percent_Identity=40.9594095940959, Blast_Score=216, Evalue=2e-56,
Organism=Homo sapiens, GI61835204, Length=271, Percent_Identity=40.9594095940959, Blast_Score=216, Evalue=2e-56,
Organism=Homo sapiens, GI194473681, Length=271, Percent_Identity=40.9594095940959, Blast_Score=216, Evalue=2e-56,
Organism=Homo sapiens, GI17402865, Length=277, Percent_Identity=39.3501805054152, Blast_Score=191, Evalue=8e-49,
Organism=Escherichia coli, GI87082121, Length=272, Percent_Identity=39.7058823529412, Blast_Score=199, Evalue=2e-52,
Organism=Escherichia coli, GI87081967, Length=302, Percent_Identity=25.1655629139073, Blast_Score=76, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17561888, Length=303, Percent_Identity=28.7128712871287, Blast_Score=101, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI115534702, Length=236, Percent_Identity=29.6610169491525, Blast_Score=93, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI71997283, Length=306, Percent_Identity=27.7777777777778, Blast_Score=92, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17559150, Length=269, Percent_Identity=24.907063197026, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17543836, Length=268, Percent_Identity=25, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17543838, Length=268, Percent_Identity=25, Blast_Score=69, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6324825, Length=270, Percent_Identity=28.8888888888889, Blast_Score=111, Evalue=2e-25,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001763
- InterPro:   IPR001307 [H]

Pfam domain/function: PF00581 Rhodanese [H]

EC number: =2.8.1.2 [H]

Molecular weight: Translated: 30807; Mature: 30807

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: PS00683 RHODANESE_2 ; PS50206 RHODANESE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQQAGAQAKMDSLVSTQWLANEMGASDLRIVDATAFLPEHGRNALLEYEACHIPGAVFMD
CCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHCCCCCCCCEEEEHHHCCCHHHHHH
LADLVDSASAVPNTLPPAEKFASRMQALGLGDGSRVVIYDDSPIKSATRAWFMLTMFGAQ
HHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCC
NVALLDGGIAKWKAEGRKCAQGRETLRARHFTVWSDQSHVRTKGDVLANLDTKAEQVVDA
CEEEECCCHHHHHHCCHHHHHHHHHHHHHHEEEECCCCCCCCCCCEEECCCHHHHHHHHH
RGAGRFTGEMAETNPAVASGHIPGARNVPYSSLFNADGTWKSPDAIRAIFEAAGVDLSRP
CCCCCCCCCHHHCCCCEECCCCCCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHCCCCCHH
LISSCGSGMTANVVIFALHLIGKDDVSLYDGSWSEWGVDPETPKATGPA
HHHHCCCCCHHHHHHHHHHHHCCCCCEEECCCCHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MQQAGAQAKMDSLVSTQWLANEMGASDLRIVDATAFLPEHGRNALLEYEACHIPGAVFMD
CCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHCCCCCCCCEEEEHHHCCCHHHHHH
LADLVDSASAVPNTLPPAEKFASRMQALGLGDGSRVVIYDDSPIKSATRAWFMLTMFGAQ
HHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCC
NVALLDGGIAKWKAEGRKCAQGRETLRARHFTVWSDQSHVRTKGDVLANLDTKAEQVVDA
CEEEECCCHHHHHHCCHHHHHHHHHHHHHHEEEECCCCCCCCCCCEEECCCHHHHHHHHH
RGAGRFTGEMAETNPAVASGHIPGARNVPYSSLFNADGTWKSPDAIRAIFEAAGVDLSRP
CCCCCCCCCHHHCCCCEECCCCCCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHCCCCCHH
LISSCGSGMTANVVIFALHLIGKDDVSLYDGSWSEWGVDPETPKATGPA
HHHHCCCCCHHHHHHHHHHHHCCCCCEEECCCCHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]