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The map label for this gene is nusA

Identifier: 87200505

GI number: 87200505

Start: 2693350

End: 2694984

Strand: Reverse

Name: nusA

Synonym: Saro_2492

Alternate gene names: 87200505

Gene position: 2694984-2693350 (Counterclockwise)

Preceding gene: 87200506

Following gene: 87200504

Centisome position: 75.67

GC content: 65.5

Gene sequence:

>1635_bases
ATGGCCAGTGCAATTTCCGCGAACCGCGCCGAACTGCTTGCGATCGCCAACGCGGTCGCCACCGAGAAGATGATCGACAA
GTCGATCGTCATCGAGGCGATGGAAGAGGCGATCCAGAAGTCCGCGCGCAACCGCTACGGCGCCGAGAACGACATTCGCG
CCAAGCTCGACCCGCGCACCGGCGACCTGCGCCTGTGGCGCGTGGTGGAAGTGGTCGAGGTGGTCGAGGACTACTTCAAG
CAGGTCGATCTCAAGCAGGCCGAGAAGCTCCAGCCCGGCGCCAAGATCGGCGACTTCATCGTCGATCCGCTGCCCCCGGT
CGATCTCGGCCGCATCGACGCGCAGTCGGCCAAGCAGGTCATCTTCCAGAAGGTCCGCGACGCCGAGCGTGATCGCCAGT
ACGACGAGTTCAAGGACCGCGCCGGCGAAGTCATCACCGGCGTGATCAAGTCGGTCGAATTCGGCCACGTGATCGTCAAC
CTCGGCCGCGCCGAAGGCGTGATCCGCCGCGACCAGCAGATCCCCCGCGAAGTGCCCCGCGTGGGCGAGCGCGTGCGTGC
GCTGATCCTCAAGGTCGAGCGCCAGAACCGCGGTCCGCAGATCTTCCTGTCCCGCGCGCACCCCGAATTCATGAAGAAGC
TCTTCGCGCAGGAAGTGCCCGAGATCTACGATGGCATCATCGAGATCAAGGCCGCCGCCCGCGACCCGGGCTCGCGCGCC
AAGATCGGCGTGATCAGCCGCGACAGCAGCATCGACCCGGTCGGCGCCTGCGTCGGTATGAAGGGTAGCCGCGTCCAGGC
GGTCGTGCAGGAACTGCAGGGCGAGAAGATCGACATCATCCCCTGGAGCGAGGACACCGCGACTTTCGTCGTCAACGCTC
TCCAGCCCGCGACCGTCAGCCGCGTGGTGATCGACGAGGAAGAGAGCCGCATCGAGGTCGTCGTGCCCGATGACCAGCTC
TCGCTCGCCATCGGTCGCCGCGGTCAGAACGTGCGCCTTGCCTCGTCGCTGACGGGCTCGGCCATCGACATCATGACCGA
GGCGGAAGCTTCGGAGAAGCGCCAGAAGGAATTCGCCGAGCGCTCGAAGATGTTCGAGGAAGAGCTCGACGTCGACGAAA
CCCTCTCGCAGCTCCTCGTCGCCGAAGGCTTCACCGAGCTGGAGGAAGTGGCCTACATCGAAATGGCCGAACTGGCCGCG
ATCGAGGGCTTCGACGAGGAACTCGCCGAGGAACTGCAGAGCCGCGCGTCCGAGGCGATCGAACGCCGCGAGGAGGCTCT
GCGCGAGCAGCGTCGTGCCCTTGGCGTCGACGATGCCCTGGCCGAACTGCCGCACCTGACCGAAGCGATGCTCGTCGCGC
TCGGCAAGGCCGGCATCAAGACGCTCGACGATCTTGCCGATCTCGCGACCGATGAACTCATCGCCAAGAAGCGCGCCGAA
CAGCGTCGCCGCAACGACAAGGGCCCGCGCGAGCGGACCGAGCGTTCGGAGCGGACAGAGGACAAGGGCGGCGTGCTCGG
CGAATTTGGCCTGAGCGAAGAACAGGGCAACGAGATCATCATGGCCGCGCGTGCCCACTGGTTCGAAGACGAACCGGTAG
CTGAGGAGGCCGCCGATGCGGATTCCTCACAATGA

Upstream 100 bases:

>100_bases
CAATGCCAAGCTTATCCTCACCGACAAGCTTATCGCCGCCTCCCGACCGCTGGATACCAGCGGTGCGGACGAAATTCTCG
AAGAACAGGAAGACTGACCA

Downstream 100 bases:

>100_bases
GCCGCTGAGCTCCGACATCGACGGCAAGGCACCGGCGGGCAAGTCCCCGCCGGAGCGCAAGTGCGTGCTGAGCGGGCGAC
ATGATGCGCGTGGCGATCTG

Product: transcription elongation factor NusA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 544; Mature: 543

Protein sequence:

>544_residues
MASAISANRAELLAIANAVATEKMIDKSIVIEAMEEAIQKSARNRYGAENDIRAKLDPRTGDLRLWRVVEVVEVVEDYFK
QVDLKQAEKLQPGAKIGDFIVDPLPPVDLGRIDAQSAKQVIFQKVRDAERDRQYDEFKDRAGEVITGVIKSVEFGHVIVN
LGRAEGVIRRDQQIPREVPRVGERVRALILKVERQNRGPQIFLSRAHPEFMKKLFAQEVPEIYDGIIEIKAAARDPGSRA
KIGVISRDSSIDPVGACVGMKGSRVQAVVQELQGEKIDIIPWSEDTATFVVNALQPATVSRVVIDEEESRIEVVVPDDQL
SLAIGRRGQNVRLASSLTGSAIDIMTEAEASEKRQKEFAERSKMFEEELDVDETLSQLLVAEGFTELEEVAYIEMAELAA
IEGFDEELAEELQSRASEAIERREEALREQRRALGVDDALAELPHLTEAMLVALGKAGIKTLDDLADLATDELIAKKRAE
QRRRNDKGPRERTERSERTEDKGGVLGEFGLSEEQGNEIIMAARAHWFEDEPVAEEAADADSSQ

Sequences:

>Translated_544_residues
MASAISANRAELLAIANAVATEKMIDKSIVIEAMEEAIQKSARNRYGAENDIRAKLDPRTGDLRLWRVVEVVEVVEDYFK
QVDLKQAEKLQPGAKIGDFIVDPLPPVDLGRIDAQSAKQVIFQKVRDAERDRQYDEFKDRAGEVITGVIKSVEFGHVIVN
LGRAEGVIRRDQQIPREVPRVGERVRALILKVERQNRGPQIFLSRAHPEFMKKLFAQEVPEIYDGIIEIKAAARDPGSRA
KIGVISRDSSIDPVGACVGMKGSRVQAVVQELQGEKIDIIPWSEDTATFVVNALQPATVSRVVIDEEESRIEVVVPDDQL
SLAIGRRGQNVRLASSLTGSAIDIMTEAEASEKRQKEFAERSKMFEEELDVDETLSQLLVAEGFTELEEVAYIEMAELAA
IEGFDEELAEELQSRASEAIERREEALREQRRALGVDDALAELPHLTEAMLVALGKAGIKTLDDLADLATDELIAKKRAE
QRRRNDKGPRERTERSERTEDKGGVLGEFGLSEEQGNEIIMAARAHWFEDEPVAEEAADADSSQ
>Mature_543_residues
ASAISANRAELLAIANAVATEKMIDKSIVIEAMEEAIQKSARNRYGAENDIRAKLDPRTGDLRLWRVVEVVEVVEDYFKQ
VDLKQAEKLQPGAKIGDFIVDPLPPVDLGRIDAQSAKQVIFQKVRDAERDRQYDEFKDRAGEVITGVIKSVEFGHVIVNL
GRAEGVIRRDQQIPREVPRVGERVRALILKVERQNRGPQIFLSRAHPEFMKKLFAQEVPEIYDGIIEIKAAARDPGSRAK
IGVISRDSSIDPVGACVGMKGSRVQAVVQELQGEKIDIIPWSEDTATFVVNALQPATVSRVVIDEEESRIEVVVPDDQLS
LAIGRRGQNVRLASSLTGSAIDIMTEAEASEKRQKEFAERSKMFEEELDVDETLSQLLVAEGFTELEEVAYIEMAELAAI
EGFDEELAEELQSRASEAIERREEALREQRRALGVDDALAELPHLTEAMLVALGKAGIKTLDDLADLATDELIAKKRAEQ
RRRNDKGPRERTERSERTEDKGGVLGEFGLSEEQGNEIIMAARAHWFEDEPVAEEAADADSSQ

Specific function: Participates in both the termination and antitermination of transcription. Interacts with RNA polymerase and binds RNA [H]

COG id: COG0195

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1789560, Length=527, Percent_Identity=40.607210626186, Blast_Score=362, Evalue=1e-101,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1612 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010995
- InterPro:   IPR004087
- InterPro:   IPR015946
- InterPro:   IPR009019
- InterPro:   IPR004088
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967
- InterPro:   IPR013735
- InterPro:   IPR010214
- InterPro:   IPR010213 [H]

Pfam domain/function: PF08529 NusA_N; PF00575 S1 [H]

EC number: NA

Molecular weight: Translated: 60510; Mature: 60379

Theoretical pI: Translated: 4.48; Mature: 4.48

Prosite motif: PS50084 KH_TYPE_1 ; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASAISANRAELLAIANAVATEKMIDKSIVIEAMEEAIQKSARNRYGAENDIRAKLDPRT
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCC
GDLRLWRVVEVVEVVEDYFKQVDLKQAEKLQPGAKIGDFIVDPLPPVDLGRIDAQSAKQV
CCHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCHHHHHCCCCCCCCCCCCCHHHHHHH
IFQKVRDAERDRQYDEFKDRAGEVITGVIKSVEFGHVIVNLGRAEGVIRRDQQIPREVPR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCHHHHHHHHHHHHHHHHHH
VGERVRALILKVERQNRGPQIFLSRAHPEFMKKLFAQEVPEIYDGIIEIKAAARDPGSRA
HHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
KIGVISRDSSIDPVGACVGMKGSRVQAVVQELQGEKIDIIPWSEDTATFVVNALQPATVS
EEEEEECCCCCCHHHHHHCCCCHHHHHHHHHHCCCEEEEEECCCCCHHHHHHCCCCCHHH
RVVIDEEESRIEVVVPDDQLSLAIGRRGQNVRLASSLTGSAIDIMTEAEASEKRQKEFAE
EEEEECCCCEEEEEECCCCEEHHCCCCCCCEEEHHCCCCCHHHHHHHHHHHHHHHHHHHH
RSKMFEEELDVDETLSQLLVAEGFTELEEVAYIEMAELAAIEGFDEELAEELQSRASEAI
HHHHHHHHCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
ERREEALREQRRALGVDDALAELPHLTEAMLVALGKAGIKTLDDLADLATDELIAKKRAE
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QRRRNDKGPRERTERSERTEDKGGVLGEFGLSEEQGNEIIMAARAHWFEDEPVAEEAADA
HHHCCCCCCHHHHHHHHCCHHCCCEEECCCCCCCCCCEEEEEEHHHCCCCCCHHHHHCCC
DSSQ
CCCC
>Mature Secondary Structure 
ASAISANRAELLAIANAVATEKMIDKSIVIEAMEEAIQKSARNRYGAENDIRAKLDPRT
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCC
GDLRLWRVVEVVEVVEDYFKQVDLKQAEKLQPGAKIGDFIVDPLPPVDLGRIDAQSAKQV
CCHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCHHHHHCCCCCCCCCCCCCHHHHHHH
IFQKVRDAERDRQYDEFKDRAGEVITGVIKSVEFGHVIVNLGRAEGVIRRDQQIPREVPR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCHHHHHHHHHHHHHHHHHH
VGERVRALILKVERQNRGPQIFLSRAHPEFMKKLFAQEVPEIYDGIIEIKAAARDPGSRA
HHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
KIGVISRDSSIDPVGACVGMKGSRVQAVVQELQGEKIDIIPWSEDTATFVVNALQPATVS
EEEEEECCCCCCHHHHHHCCCCHHHHHHHHHHCCCEEEEEECCCCCHHHHHHCCCCCHHH
RVVIDEEESRIEVVVPDDQLSLAIGRRGQNVRLASSLTGSAIDIMTEAEASEKRQKEFAE
EEEEECCCCEEEEEECCCCEEHHCCCCCCCEEEHHCCCCCHHHHHHHHHHHHHHHHHHHH
RSKMFEEELDVDETLSQLLVAEGFTELEEVAYIEMAELAAIEGFDEELAEELQSRASEAI
HHHHHHHHCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
ERREEALREQRRALGVDDALAELPHLTEAMLVALGKAGIKTLDDLADLATDELIAKKRAE
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QRRRNDKGPRERTERSERTEDKGGVLGEFGLSEEQGNEIIMAARAHWFEDEPVAEEAADA
HHHCCCCCCHHHHHHHHCCHHCCCEEECCCCCCCCCCEEEEEEHHHCCCCCCHHHHHCCC
DSSQ
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11557893 [H]