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The map label for this gene is rph

Identifier: 87200077

GI number: 87200077

Start: 2201499

End: 2202182

Strand: Direct

Name: rph

Synonym: Saro_2061

Alternate gene names: 87200077

Gene position: 2201499-2202182 (Clockwise)

Preceding gene: 87200074

Following gene: 87200078

Centisome position: 61.81

GC content: 66.67

Gene sequence:

>684_bases
ATGCGCGCCATCTCCATCGAAACCGCCTTCACCAAGCATGCCGAAGGCTCGGTCCTGATCTGCTTCGGCGATACCAAGGT
ACTCGTCACCGCCTCGGTGGAAGAGCGCGTGCCGCCATTCCTGCGCGGCAAGGGCGAAGGCTGGGTCACGGCGGAATACT
CGATGCTGCCGCGCGCCACCCATACCCGTGGCAGCCGCGAGGCGGCCAAGGGCAAGCAGTCGGGCCGCACCCAGGAAATC
CAGCGCCTCATCGGCCGCAGCCTGCGTGCCGTGACCGATCTCAAGAAGCTGGGCGAACGCCAGATCACGCTCGATTGCGA
CGTGATCCAGGCGGACGGCGGCACCCGCACCGCCGCCATTTCCGGCGCATGGGTCGCGCTGCGCCTTGCCGTCCAGAAGC
TGCTCGATAGCGGCGCGATCAAGGAAGACCCGCTGACCGAAAAGGTCGCGGCGGTGTCCTGCGGCATCGTCAACGGCCAG
CCTGTGCTCGATCTCGACTATATCGAGGATTCCTCGGCGGATGCCGACGCTAACTTCGTCCTGATCGAAGGCGGCAAGAT
CGCAGAGGCCCAGGCCACCGCCGAAGGCGCGACCTACGACGAGGAAGGCCTCCTGCGCCTGCTGCGCCTCGCCCGGATGG
GCTGCGCGGAAATCTTCGCCGCGCAGGAAAAGGCGGTCCGCTGA

Upstream 100 bases:

>100_bases
CTATTCACCGCTCTTGCAGGCTCAAGAGCAATGCGCCAGAGGCCGCGCAAACTTGCGAGGGAAATCTTTCATGCGACCTT
CCGGCCGCGCGCCTGATGAA

Downstream 100 bases:

>100_bases
TGTCTTCGCCCCGTCTCGGTTCCGGAAAGCTGGTGATTGCCACGCACAATTCCGGAAAGCTCAAGGAAATCCAGGCCCTG
CTCGCCCCCTATGGCATCGA

Product: ribonuclease PH

Products: NA

Alternate protein names: RNase PH; tRNA nucleotidyltransferase [H]

Number of amino acids: Translated: 227; Mature: 227

Protein sequence:

>227_residues
MRAISIETAFTKHAEGSVLICFGDTKVLVTASVEERVPPFLRGKGEGWVTAEYSMLPRATHTRGSREAAKGKQSGRTQEI
QRLIGRSLRAVTDLKKLGERQITLDCDVIQADGGTRTAAISGAWVALRLAVQKLLDSGAIKEDPLTEKVAAVSCGIVNGQ
PVLDLDYIEDSSADADANFVLIEGGKIAEAQATAEGATYDEEGLLRLLRLARMGCAEIFAAQEKAVR

Sequences:

>Translated_227_residues
MRAISIETAFTKHAEGSVLICFGDTKVLVTASVEERVPPFLRGKGEGWVTAEYSMLPRATHTRGSREAAKGKQSGRTQEI
QRLIGRSLRAVTDLKKLGERQITLDCDVIQADGGTRTAAISGAWVALRLAVQKLLDSGAIKEDPLTEKVAAVSCGIVNGQ
PVLDLDYIEDSSADADANFVLIEGGKIAEAQATAEGATYDEEGLLRLLRLARMGCAEIFAAQEKAVR
>Mature_227_residues
MRAISIETAFTKHAEGSVLICFGDTKVLVTASVEERVPPFLRGKGEGWVTAEYSMLPRATHTRGSREAAKGKQSGRTQEI
QRLIGRSLRAVTDLKKLGERQITLDCDVIQADGGTRTAAISGAWVALRLAVQKLLDSGAIKEDPLTEKVAAVSCGIVNGQ
PVLDLDYIEDSSADADANFVLIEGGKIAEAQATAEGATYDEEGLLRLLRLARMGCAEIFAAQEKAVR

Specific function: Phosphorolytic exoribonuclease that removes nucleotide residues following the -CCA terminus of tRNA and adds nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates [H]

COG id: COG0689

COG function: function code J; RNase PH

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase PH family [H]

Homologues:

Organism=Escherichia coli, GI157672248, Length=199, Percent_Identity=64.321608040201, Blast_Score=266, Evalue=1e-72,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR020568
- InterPro:   IPR002381
- InterPro:   IPR018336 [H]

Pfam domain/function: PF01138 RNase_PH; PF03725 RNase_PH_C [H]

EC number: =2.7.7.56 [H]

Molecular weight: Translated: 24329; Mature: 24329

Theoretical pI: Translated: 5.45; Mature: 5.45

Prosite motif: PS01277 RIBONUCLEASE_PH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRAISIETAFTKHAEGSVLICFGDTKVLVTASVEERVPPFLRGKGEGWVTAEYSMLPRAT
CCEEEEEHHHHHCCCCCEEEEECCCEEEEEECHHHHCCHHHCCCCCCEEEEEHHHCCCCC
HTRGSREAAKGKQSGRTQEIQRLIGRSLRAVTDLKKLGERQITLDCDVIQADGGTRTAAI
CCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEECCCCCEEEEE
SGAWVALRLAVQKLLDSGAIKEDPLTEKVAAVSCGIVNGQPVLDLDYIEDSSADADANFV
CHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHEEEEECCCCEEEEECCCCCCCCCCCCEE
LIEGGKIAEAQATAEGATYDEEGLLRLLRLARMGCAEIFAAQEKAVR
EEECCCEECHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRAISIETAFTKHAEGSVLICFGDTKVLVTASVEERVPPFLRGKGEGWVTAEYSMLPRAT
CCEEEEEHHHHHCCCCCEEEEECCCEEEEEECHHHHCCHHHCCCCCCEEEEEHHHCCCCC
HTRGSREAAKGKQSGRTQEIQRLIGRSLRAVTDLKKLGERQITLDCDVIQADGGTRTAAI
CCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEECCCCCEEEEE
SGAWVALRLAVQKLLDSGAIKEDPLTEKVAAVSCGIVNGQPVLDLDYIEDSSADADANFV
CHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHEEEEECCCCEEEEECCCCCCCCCCCCEE
LIEGGKIAEAQATAEGATYDEEGLLRLLRLARMGCAEIFAAQEKAVR
EEECCCEECHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA