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The map label for this gene is rutB [H]

Identifier: 87200074

GI number: 87200074

Start: 2198985

End: 2199662

Strand: Direct

Name: rutB [H]

Synonym: Saro_2058

Alternate gene names: 87200074

Gene position: 2198985-2199662 (Clockwise)

Preceding gene: 87200072

Following gene: 87200077

Centisome position: 61.74

GC content: 66.67

Gene sequence:

>678_bases
ATGCCACATGCCGTCGATCACCACGCAATTCTCGCCCGCTTCGCACCTTCACGGGGCGGCGACGGCAACATCTTCCGCGA
CATCGATCCGTCCCGGCTCGCCCATATAGTGGTCGACATGCAGAACGGCTTCATGGAGGAAGGCGCACCGGTCGAGGTGC
CGGTGGCGCGCACCATCGTGGACAACATCAACCGCATCTCGGCCGCCGTCCGCGATGCCGGCGGGCACAACGTCTTCCTG
CGCTACACGACCCCGCCCGAAGGCGGCCCAAGCTGGTCGAACTTCATGGTGCGCATGGGCCGCGAGGCGCAAGGCCACCG
CGAAGCCTTCACGCCGGGCGCGCACCACTGGCAGCTCTGGCCGACGCTGGACGTCCGAGAAGCCGACGCAATCGTCGACA
AGCACCGCTTCAGCGGCTTCACGCCGGGCACCTGCGCCCTGAAAGACGTCCTCGACGCGCGCGGCATCGACACCGTGCTC
ATCACCGGCACGCTCACCAACTGCTGCTGCGAAAGCACCGCCCGCGACGCGATGCAGCACAATTACCGCGTGCTGATGGC
GAGCGACGCCAACGCTGCCCTCTCGGACGAGGAACACGCCGCCACCCTGCACATCATGGCCATGGTCTTCGCCGATCTCC
ACTCGACGCAGGAAGTCGTCGACCTGCTGGGCAGCTGA

Upstream 100 bases:

>100_bases
CGGTGCGCCCGCACGTTTCTCCTGCCGAAATGCCATGGTGCCAGCCCCGGACAAACGGAGGCGCATCGGCCACAATGGCG
CTTCATAGGGAGAACAGCCC

Downstream 100 bases:

>100_bases
AAAGAGAAGCCCCCGCGCAGGCGGGGGCTCCGGGTTGCGTTACGGAACGCAATTACAGGCCGTCCCGGCCCCCGCCTGAA
CGGAGACCCGACGGCGCAGA

Product: isochorismatase hydrolase

Products: NA

Alternate protein names: Ureidoacrylate amidohydrolase [H]

Number of amino acids: Translated: 225; Mature: 224

Protein sequence:

>225_residues
MPHAVDHHAILARFAPSRGGDGNIFRDIDPSRLAHIVVDMQNGFMEEGAPVEVPVARTIVDNINRISAAVRDAGGHNVFL
RYTTPPEGGPSWSNFMVRMGREAQGHREAFTPGAHHWQLWPTLDVREADAIVDKHRFSGFTPGTCALKDVLDARGIDTVL
ITGTLTNCCCESTARDAMQHNYRVLMASDANAALSDEEHAATLHIMAMVFADLHSTQEVVDLLGS

Sequences:

>Translated_225_residues
MPHAVDHHAILARFAPSRGGDGNIFRDIDPSRLAHIVVDMQNGFMEEGAPVEVPVARTIVDNINRISAAVRDAGGHNVFL
RYTTPPEGGPSWSNFMVRMGREAQGHREAFTPGAHHWQLWPTLDVREADAIVDKHRFSGFTPGTCALKDVLDARGIDTVL
ITGTLTNCCCESTARDAMQHNYRVLMASDANAALSDEEHAATLHIMAMVFADLHSTQEVVDLLGS
>Mature_224_residues
PHAVDHHAILARFAPSRGGDGNIFRDIDPSRLAHIVVDMQNGFMEEGAPVEVPVARTIVDNINRISAAVRDAGGHNVFLR
YTTPPEGGPSWSNFMVRMGREAQGHREAFTPGAHHWQLWPTLDVREADAIVDKHRFSGFTPGTCALKDVLDARGIDTVLI
TGTLTNCCCESTARDAMQHNYRVLMASDANAALSDEEHAATLHIMAMVFADLHSTQEVVDLLGS

Specific function: In vivo, quickly hydrolyzes the ureidoacrylate peracid to avoid toxicity, but can also hydrolyzes ureidoacrylate that is formed spontaneously from ureidoacrylate peracid. One of the products of hydrolysis, carbamate, hydrolyzes spontaneously, thereby rele

COG id: COG1335

COG function: function code Q; Amidases related to nicotinamidase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isochorismatase family. RutB subfamily [H]

Homologues:

Organism=Escherichia coli, GI87081820, Length=180, Percent_Identity=31.6666666666667, Blast_Score=74, Evalue=8e-15,
Organism=Escherichia coli, GI87081992, Length=199, Percent_Identity=26.1306532663317, Blast_Score=61, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019916
- InterPro:   IPR000868 [H]

Pfam domain/function: PF00857 Isochorismatase [H]

EC number: NA

Molecular weight: Translated: 24576; Mature: 24445

Theoretical pI: Translated: 5.85; Mature: 5.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPHAVDHHAILARFAPSRGGDGNIFRDIDPSRLAHIVVDMQNGFMEEGAPVEVPVARTIV
CCCCCCHHHHHHHHCCCCCCCCCEECCCCHHHHEEEEEECCCCCCCCCCCEECHHHHHHH
DNINRISAAVRDAGGHNVFLRYTTPPEGGPSWSNFMVRMGREAQGHREAFTPGAHHWQLW
HHHHHHHHHHHCCCCCEEEEEECCCCCCCCCHHHHHHHHCCCCCCCHHHCCCCCCCEEEC
PTLDVREADAIVDKHRFSGFTPGTCALKDVLDARGIDTVLITGTLTNCCCESTARDAMQH
CCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHC
NYRVLMASDANAALSDEEHAATLHIMAMVFADLHSTQEVVDLLGS
CCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHCC
>Mature Secondary Structure 
PHAVDHHAILARFAPSRGGDGNIFRDIDPSRLAHIVVDMQNGFMEEGAPVEVPVARTIV
CCCCCHHHHHHHHCCCCCCCCCEECCCCHHHHEEEEEECCCCCCCCCCCEECHHHHHHH
DNINRISAAVRDAGGHNVFLRYTTPPEGGPSWSNFMVRMGREAQGHREAFTPGAHHWQLW
HHHHHHHHHHHCCCCCEEEEEECCCCCCCCCHHHHHHHHCCCCCCCHHHCCCCCCCEEEC
PTLDVREADAIVDKHRFSGFTPGTCALKDVLDARGIDTVLITGTLTNCCCESTARDAMQH
CCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHC
NYRVLMASDANAALSDEEHAATLHIMAMVFADLHSTQEVVDLLGS
CCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA