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The map label for this gene is lexA

Identifier: 87200045

GI number: 87200045

Start: 2162774

End: 2163478

Strand: Direct

Name: lexA

Synonym: Saro_2029

Alternate gene names: 87200045

Gene position: 2162774-2163478 (Clockwise)

Preceding gene: 87200044

Following gene: 87200047

Centisome position: 60.73

GC content: 65.82

Gene sequence:

>705_bases
ATGTTGACGCGCAAGCAGCATGAACTGCTCACCTTCATCCAGACCCGGCTGGAAGATTCCGGTATCTCGCCCTCGTTCGA
GGAGATGAAGGAAGCGCTCGACCTCAAGTCGAAGTCCGGCGTCCACCGCCTGATCTCTGCGCTCGAGGAACGCGGCTTCA
TCCGCCGCCTGCCCAACCGCGCCCGCGCGCTCGAAGTCCTGCGCCAGCCCGATAGCGCCGTTGGCAAGGCCGCGCCTGTT
TCGCAGCGCGAGGCGGCGAACACGAATTCCGCCCTTCCCCCGCTGCGCGCCGCGCCCAAGGCTGCACCTGCGCCGGCGAA
CGACGTGATCGAACTGCCGCTCCACGGCAAGATCGCCGCGGGCGTCCCGATAGAAGCGCTGGAAACCACGGCAACGTTGC
CGGTCCCGGCGGCCCTGCTCGGCGCCGGCGAACACTATGCGCTCGAAGTTTCCGGTGATTCCATGGTCGAAGCCGGCATC
TTCGATGGCGACTATGCCCTCGTCCGCAAGACCGACGTTGCGCGCGACGGCGAAATCGTGGTTGCGCTCGTTCGCGGAGA
GGAAGCGACGCTGAAGTACCTGCACCGCGAAAAGGGCATGGTCCGCCTCGATCCTGCGAATGCGGCCTACGACCCGCAGT
ATTACCGCCCGGAAGAAGTGGCCGTGCAGGGCAAGCTCGCCGGATTGCTGCGCCGCTACCATTGA

Upstream 100 bases:

>100_bases
CTTATCCGTTCCACATTCGTTCACCGGAACATGCACCCCGTTCCGGTCTATGGAAGACCCGTGGTTTCGCCATGAAACCA
CCATTGCGGAGTGGCCGCCG

Downstream 100 bases:

>100_bases
CCCCCTGCCACTGAGCCCCTGCCACTGAGCCCCTGCCGCTGAGTTCCTGACGCAGGGCTCCGGTCACGGGGCCGAACCGT
GCGGGGTTGCGCTTCGGCCG

Product: LexA repressor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 234; Mature: 234

Protein sequence:

>234_residues
MLTRKQHELLTFIQTRLEDSGISPSFEEMKEALDLKSKSGVHRLISALEERGFIRRLPNRARALEVLRQPDSAVGKAAPV
SQREAANTNSALPPLRAAPKAAPAPANDVIELPLHGKIAAGVPIEALETTATLPVPAALLGAGEHYALEVSGDSMVEAGI
FDGDYALVRKTDVARDGEIVVALVRGEEATLKYLHREKGMVRLDPANAAYDPQYYRPEEVAVQGKLAGLLRRYH

Sequences:

>Translated_234_residues
MLTRKQHELLTFIQTRLEDSGISPSFEEMKEALDLKSKSGVHRLISALEERGFIRRLPNRARALEVLRQPDSAVGKAAPV
SQREAANTNSALPPLRAAPKAAPAPANDVIELPLHGKIAAGVPIEALETTATLPVPAALLGAGEHYALEVSGDSMVEAGI
FDGDYALVRKTDVARDGEIVVALVRGEEATLKYLHREKGMVRLDPANAAYDPQYYRPEEVAVQGKLAGLLRRYH
>Mature_234_residues
MLTRKQHELLTFIQTRLEDSGISPSFEEMKEALDLKSKSGVHRLISALEERGFIRRLPNRARALEVLRQPDSAVGKAAPV
SQREAANTNSALPPLRAAPKAAPAPANDVIELPLHGKIAAGVPIEALETTATLPVPAALLGAGEHYALEVSGDSMVEAGI
FDGDYALVRKTDVARDGEIVVALVRGEEATLKYLHREKGMVRLDPANAAYDPQYYRPEEVAVQGKLAGLLRRYH

Specific function: Represses a number of genes involved in the response to DNA damage (SOS response), including recA and lexA. In the presence of single-stranded DNA, recA interacts with lexA causing an autocatalytic cleavage which disrupts the DNA-binding part of lexA, lea

COG id: COG1974

COG function: function code KT; SOS-response transcriptional repressors (RecA-mediated autopeptidases)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S24 family

Homologues:

Organism=Escherichia coli, GI1790476, Length=233, Percent_Identity=25.3218884120172, Blast_Score=74, Evalue=6e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LEXA_NOVAD (Q2G6Q5)

Other databases:

- EMBL:   CP000248
- RefSeq:   YP_497302.1
- ProteinModelPortal:   Q2G6Q5
- SMR:   Q2G6Q5
- STRING:   Q2G6Q5
- MEROPS:   S24.001
- GeneID:   3917676
- GenomeReviews:   CP000248_GR
- KEGG:   nar:Saro_2029
- NMPDR:   fig|48935.1.peg.2295
- eggNOG:   COG1974
- HOGENOM:   HBG679610
- OMA:   LMFIHER
- PhylomeDB:   Q2G6Q5
- ProtClustDB:   PRK00215
- BioCyc:   NARO279238:SARO_2029-MONOMER
- GO:   GO:0006508
- GO:   GO:0006350
- HAMAP:   MF_00015
- InterPro:   IPR006199
- InterPro:   IPR006200
- InterPro:   IPR006197
- InterPro:   IPR019759
- InterPro:   IPR015927
- InterPro:   IPR011056
- InterPro:   IPR011991
- Gene3D:   G3DSA:2.10.109.10
- Gene3D:   G3DSA:1.10.10.10
- PRINTS:   PR00726
- TIGRFAMs:   TIGR00498

Pfam domain/function: PF01726 LexA_DNA_bind; PF00717 Peptidase_S24; SSF51306 Pept_S24_S26_C

EC number: =3.4.21.88

Molecular weight: Translated: 25414; Mature: 25414

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: NA

Important sites: ACT_SITE 154-154 ACT_SITE 192-192

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTRKQHELLTFIQTRLEDSGISPSFEEMKEALDLKSKSGVHRLISALEERGFIRRLPNR
CCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHCCHHHHCCHH
ARALEVLRQPDSAVGKAAPVSQREAANTNSALPPLRAAPKAAPAPANDVIELPLHGKIAA
HHHHHHHHCCCHHHCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCEEC
GVPIEALETTATLPVPAALLGAGEHYALEVSGDSMVEAGIFDGDYALVRKTDVARDGEIV
CCCHHHHHHCCCCCCCHHHHCCCCEEEEEECCCCEEECCCCCCCEEEEEECCCCCCCCEE
VALVRGEEATLKYLHREKGMVRLDPANAAYDPQYYRPEEVAVQGKLAGLLRRYH
EEEEECCHHHHHHHHHHCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLTRKQHELLTFIQTRLEDSGISPSFEEMKEALDLKSKSGVHRLISALEERGFIRRLPNR
CCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHCCHHHHCCHH
ARALEVLRQPDSAVGKAAPVSQREAANTNSALPPLRAAPKAAPAPANDVIELPLHGKIAA
HHHHHHHHCCCHHHCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCEEC
GVPIEALETTATLPVPAALLGAGEHYALEVSGDSMVEAGIFDGDYALVRKTDVARDGEIV
CCCHHHHHHCCCCCCCHHHHCCCCEEEEEECCCCEEECCCCCCCEEEEEECCCCCCCCEE
VALVRGEEATLKYLHREKGMVRLDPANAAYDPQYYRPEEVAVQGKLAGLLRRYH
EEEEECCHHHHHHHHHHCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA