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The map label for this gene is gltX2

Identifier: 87200047

GI number: 87200047

Start: 2165934

End: 2167397

Strand: Direct

Name: gltX2

Synonym: Saro_2031

Alternate gene names: 87200047

Gene position: 2165934-2167397 (Clockwise)

Preceding gene: 87200045

Following gene: 87200048

Centisome position: 60.81

GC content: 65.3

Gene sequence:

>1464_bases
ATGGCAAGCGCCACTGACACCGTTTCTGCAGGATCGACGCCCCGGGACAAGGTCGTCACCCGTTTCGCCCCCTCGCCCAC
GGGTTTCCTGCACATTGGCGGCGCGCGCACGGCGCTGTTCAACTGGCTCTATGCGCGCCACCATGGCGGCACCTACCTCC
TGCGCATCGAGGATACCGACCGTGCGCGTTCGACGGACGCCGCGATCGACGCGATCTTCGACGGACTCGAATGGCTGGGC
CTTGGCGGCGACGAGCCGGCGGTGTTCCAGTTCGCGCGGTCGGACCGGCACGCCGAAGTGGCGCACAAGCTGCTCGAGGC
CGGCCATGCCTATCGCTGCTATCTGACCCAGGAAGAACTGGCGGCACGGCGCGAACTGGCGCAGGCGGAACGGCGCCCGT
TCCGCATCGACAGCGAATGGCGCGATGCCACGCCCGACCAGTGGCCCGCCGATCAGTCCTATGTCGTGCGGATGAAGGCC
CCGCGCGAAGGCGAGACGACGATCGTCGACAAGGTGCAGGGTTCGATCACCGTCCAGAACAGCGAACTGGACGACTTCAT
CATCCTGCGTTCCGACGGCACGCCGACCTACATGCTGGCGGTGGTGGTGGACGACCACGACATGGGCGTGACGCACGTCA
TCCGCGGCGACGACCACATCAACAACGCGTTCCGCCAGCTCGTCATCATCCGCGGCATGCATGCCATCGAAGGCGGTTGG
CCGGACCCCGTCTATGCTCACATTCCGCTGATCCACGGCGCGGACGGGGCGAAGCTTTCAAAGCGCCACGGAGCGCTGGG
CGTCGACGCCTATCGCGACGAGATGGGGCTCCTGCCCGAGGCGGTGTTCAACTATCTGCTGCGTCTTGGCTGGGGCCACG
GCGACGAGGAAATCATCAGCCGCGAACAGGCAGTGGCGTGGTTCGATATTGGCGACGTCAACAAGGGCGCATCACGGTTC
GACCTGAAGAAGCTGCTCAACCTCAACGGGCACTATATCCGCGAGGCGGACGATGCACGCCTGGCAGCCCTGGTCGCGCC
CCGCCTGGCGACGCTGGCTCCCGGGTTCGCGCCCGACAAGGGGCTGGACCTGCTGACCCGGGCGATGCCGGTGCTGAAGG
TGCGCGCGGCGGATATCAACGAACTGGCAGCCGGTTCGGTGTTCCTTTTCGCCCAGCGTCCGCTGGCCATGGCCGAGAAG
GCAGCAAGCCTGTTGACCGACGACGCACGTGCGATCCTGACCAAGGTTGCAGGGGTCCTGGAGGCTGAAAACGTCTGGAC
AACCGGAGTGCTCGAGGCCACGGTAAAGCAAATGGCCGAGGAGCTTGGGATAGGCTTGGGCAAGATTGCCCAACCGTTGC
GCGCAAGTTTGACGGGACAGACTACCTCGCCGGGAATTTTCGACGTATTGGCGCTGCTCGGCAAGGACGAGTCGCTGTCG
CGCATTCGCGATCAGGCGGCCTGA

Upstream 100 bases:

>100_bases
CGCGCTGGCAGAGCATCCGGATCGGCGGCAGGAAATCCTGCTGCTGGGCCAACTTCTGCCGGAGACACCATGGATTTCAA
AGGGAAGGAAAGCGCGGCAG

Downstream 100 bases:

>100_bases
CCGGGCAAGCTATTGCAAAGACACAACGAACAGGAGGGCTAAGCGTGAGCGATAACCAGGCGTCTTTGACCGCTGACGGC
AAGACCGTGGAAATGCCGGT

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase 2; GluRS 2

Number of amino acids: Translated: 487; Mature: 486

Protein sequence:

>487_residues
MASATDTVSAGSTPRDKVVTRFAPSPTGFLHIGGARTALFNWLYARHHGGTYLLRIEDTDRARSTDAAIDAIFDGLEWLG
LGGDEPAVFQFARSDRHAEVAHKLLEAGHAYRCYLTQEELAARRELAQAERRPFRIDSEWRDATPDQWPADQSYVVRMKA
PREGETTIVDKVQGSITVQNSELDDFIILRSDGTPTYMLAVVVDDHDMGVTHVIRGDDHINNAFRQLVIIRGMHAIEGGW
PDPVYAHIPLIHGADGAKLSKRHGALGVDAYRDEMGLLPEAVFNYLLRLGWGHGDEEIISREQAVAWFDIGDVNKGASRF
DLKKLLNLNGHYIREADDARLAALVAPRLATLAPGFAPDKGLDLLTRAMPVLKVRAADINELAAGSVFLFAQRPLAMAEK
AASLLTDDARAILTKVAGVLEAENVWTTGVLEATVKQMAEELGIGLGKIAQPLRASLTGQTTSPGIFDVLALLGKDESLS
RIRDQAA

Sequences:

>Translated_487_residues
MASATDTVSAGSTPRDKVVTRFAPSPTGFLHIGGARTALFNWLYARHHGGTYLLRIEDTDRARSTDAAIDAIFDGLEWLG
LGGDEPAVFQFARSDRHAEVAHKLLEAGHAYRCYLTQEELAARRELAQAERRPFRIDSEWRDATPDQWPADQSYVVRMKA
PREGETTIVDKVQGSITVQNSELDDFIILRSDGTPTYMLAVVVDDHDMGVTHVIRGDDHINNAFRQLVIIRGMHAIEGGW
PDPVYAHIPLIHGADGAKLSKRHGALGVDAYRDEMGLLPEAVFNYLLRLGWGHGDEEIISREQAVAWFDIGDVNKGASRF
DLKKLLNLNGHYIREADDARLAALVAPRLATLAPGFAPDKGLDLLTRAMPVLKVRAADINELAAGSVFLFAQRPLAMAEK
AASLLTDDARAILTKVAGVLEAENVWTTGVLEATVKQMAEELGIGLGKIAQPLRASLTGQTTSPGIFDVLALLGKDESLS
RIRDQAA
>Mature_486_residues
ASATDTVSAGSTPRDKVVTRFAPSPTGFLHIGGARTALFNWLYARHHGGTYLLRIEDTDRARSTDAAIDAIFDGLEWLGL
GGDEPAVFQFARSDRHAEVAHKLLEAGHAYRCYLTQEELAARRELAQAERRPFRIDSEWRDATPDQWPADQSYVVRMKAP
REGETTIVDKVQGSITVQNSELDDFIILRSDGTPTYMLAVVVDDHDMGVTHVIRGDDHINNAFRQLVIIRGMHAIEGGWP
DPVYAHIPLIHGADGAKLSKRHGALGVDAYRDEMGLLPEAVFNYLLRLGWGHGDEEIISREQAVAWFDIGDVNKGASRFD
LKKLLNLNGHYIREADDARLAALVAPRLATLAPGFAPDKGLDLLTRAMPVLKVRAADINELAAGSVFLFAQRPLAMAEKA
ASLLTDDARAILTKVAGVLEAENVWTTGVLEATVKQMAEELGIGLGKIAQPLRASLTGQTTSPGIFDVLALLGKDESLSR
IRDQAA

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu)

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI134288884, Length=489, Percent_Identity=28.8343558282209, Blast_Score=200, Evalue=3e-51,
Organism=Escherichia coli, GI1788743, Length=471, Percent_Identity=43.9490445859873, Blast_Score=376, Evalue=1e-105,
Organism=Escherichia coli, GI226510925, Length=250, Percent_Identity=34.8, Blast_Score=121, Evalue=9e-29,
Organism=Caenorhabditis elegans, GI71993394, Length=490, Percent_Identity=30.6122448979592, Blast_Score=198, Evalue=7e-51,
Organism=Saccharomyces cerevisiae, GI6324540, Length=502, Percent_Identity=30.0796812749004, Blast_Score=210, Evalue=5e-55,
Organism=Saccharomyces cerevisiae, GI6324742, Length=114, Percent_Identity=33.3333333333333, Blast_Score=75, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6321192, Length=216, Percent_Identity=27.7777777777778, Blast_Score=71, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24665321, Length=493, Percent_Identity=31.4401622718053, Blast_Score=216, Evalue=3e-56,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYE2_NOVAD (Q2G6Q3)

Other databases:

- EMBL:   CP000248
- RefSeq:   YP_497304.1
- ProteinModelPortal:   Q2G6Q3
- SMR:   Q2G6Q3
- STRING:   Q2G6Q3
- GeneID:   3917678
- GenomeReviews:   CP000248_GR
- KEGG:   nar:Saro_2031
- NMPDR:   fig|48935.1.peg.2297
- eggNOG:   COG0008
- HOGENOM:   HBG628189
- OMA:   NEIFAKR
- PhylomeDB:   Q2G6Q3
- BioCyc:   NARO279238:SARO_2031-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00022_B
- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729
- Gene3D:   G3DSA:1.10.1160.10
- Gene3D:   G3DSA:3.40.50.620
- Gene3D:   G3DSA:1.10.10.350
- PANTHER:   PTHR10119
- PRINTS:   PR00987
- TIGRFAMs:   TIGR00464

Pfam domain/function: PF00749 tRNA-synt_1c; SSF48163 tRNA-synt_bind

EC number: =6.1.1.17

Molecular weight: Translated: 53205; Mature: 53074

Theoretical pI: Translated: 5.60; Mature: 5.60

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 261-261

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASATDTVSAGSTPRDKVVTRFAPSPTGFLHIGGARTALFNWLYARHHGGTYLLRIEDTD
CCCCCCCCCCCCCCHHHHHEEECCCCCCEEEECCHHHHHHHHHHHEECCCEEEEEEECCC
RARSTDAAIDAIFDGLEWLGLGGDEPAVFQFARSDRHAEVAHKLLEAGHAYRCYLTQEEL
CCCHHHHHHHHHHHCHHHCCCCCCCHHHHHHHHCCCHHHHHHHHHHCCCCEEEEEEHHHH
AARRELAQAERRPFRIDSEWRDATPDQWPADQSYVVRMKAPREGETTIVDKVQGSITVQN
HHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCEEEEECCCEEEEEC
SELDDFIILRSDGTPTYMLAVVVDDHDMGVTHVIRGDDHINNAFRQLVIIRGMHAIEGGW
CCCCCEEEEECCCCCEEEEEEEEECCCCCEEEEECCCHHHHHHHHHHHHHHCCHHHCCCC
PDPVYAHIPLIHGADGAKLSKRHGALGVDAYRDEMGLLPEAVFNYLLRLGWGHGDEEIIS
CCCCEEEEEEEECCCCCCHHHHCCCCCCHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHH
REQAVAWFDIGDVNKGASRFDLKKLLNLNGHYIREADDARLAALVAPRLATLAPGFAPDK
HHHEEEEEECCCCCCCCHHHHHHHHHCCCCCEEECCCCHHHHHHHHHHHHHHCCCCCCCC
GLDLLTRAMPVLKVRAADINELAAGSVFLFAQRPLAMAEKAASLLTDDARAILTKVAGVL
CHHHHHHHHHHHEEECCCHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHH
EAENVWTTGVLEATVKQMAEELGIGLGKIAQPLRASLTGQTTSPGIFDVLALLGKDESLS
HCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCHHHH
RIRDQAA
HHHHHCC
>Mature Secondary Structure 
ASATDTVSAGSTPRDKVVTRFAPSPTGFLHIGGARTALFNWLYARHHGGTYLLRIEDTD
CCCCCCCCCCCCCHHHHHEEECCCCCCEEEECCHHHHHHHHHHHEECCCEEEEEEECCC
RARSTDAAIDAIFDGLEWLGLGGDEPAVFQFARSDRHAEVAHKLLEAGHAYRCYLTQEEL
CCCHHHHHHHHHHHCHHHCCCCCCCHHHHHHHHCCCHHHHHHHHHHCCCCEEEEEEHHHH
AARRELAQAERRPFRIDSEWRDATPDQWPADQSYVVRMKAPREGETTIVDKVQGSITVQN
HHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCEEEEECCCEEEEEC
SELDDFIILRSDGTPTYMLAVVVDDHDMGVTHVIRGDDHINNAFRQLVIIRGMHAIEGGW
CCCCCEEEEECCCCCEEEEEEEEECCCCCEEEEECCCHHHHHHHHHHHHHHCCHHHCCCC
PDPVYAHIPLIHGADGAKLSKRHGALGVDAYRDEMGLLPEAVFNYLLRLGWGHGDEEIIS
CCCCEEEEEEEECCCCCCHHHHCCCCCCHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHH
REQAVAWFDIGDVNKGASRFDLKKLLNLNGHYIREADDARLAALVAPRLATLAPGFAPDK
HHHEEEEEECCCCCCCCHHHHHHHHHCCCCCEEECCCCHHHHHHHHHHHHHHCCCCCCCC
GLDLLTRAMPVLKVRAADINELAAGSVFLFAQRPLAMAEKAASLLTDDARAILTKVAGVL
CHHHHHHHHHHHEEECCCHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHH
EAENVWTTGVLEATVKQMAEELGIGLGKIAQPLRASLTGQTTSPGIFDVLALLGKDESLS
HCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCHHHH
RIRDQAA
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA