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The map label for this gene is aglA [H]

Identifier: 87199908

GI number: 87199908

Start: 2001334

End: 2002968

Strand: Direct

Name: aglA [H]

Synonym: Saro_1891

Alternate gene names: 87199908

Gene position: 2001334-2002968 (Clockwise)

Preceding gene: 87199907

Following gene: 87199909

Centisome position: 56.19

GC content: 63.85

Gene sequence:

>1635_bases
ATGAAGCAGACCGAAACAGCCATTGCCGACCCCAAAATGCACACGGAGACGCCGTGGTGGCGCGGTGCGGCGATCTATCA
GATCTATCCACGCAGCTTTTGCGATTCCAACGGCGACGGCATCGGGGACCTGAATGGCATCGCCTCGCGACTGGATCACG
TCGCGCGCCTTGGCGTAGACGCGATCTGGATCTCGCCGTTCTTCACCTCGCCGATGAAGGACTTCGGCTACGACGTCGCC
GACTACTGCGACGTCGATCCGATCTTCGGGACGCTGGCGGATTTCGATGCCCTGGTAAAACGCGCGCACGAGCTGGGCCT
CAAGGTCACGATCGACCAGGTCTATGCGCATACCTCGGACATTCATCCGTGGTTTGCCGAAAGCCGGCAGGACCGTACCA
ACGCCAGGGCGGACTGGTATGTCTGGGCCGATCCTAAGCCCGATGGGTCGCCCCCGTCGAACTGGCAGTCGGTATTCGGC
GGTCCGGCATGGACGTGGGATGCGCGGCGCTGCCAGTATTACCTGCACAACTTCCTATCCAGCCAGCCCCAGGTAAACGC
CCACAATCCCGAAGTGCAGGAAGCGCTGCTCGGGGCGATGAAGTTCTGGCTGGACCGGGGTGTTGACGGCTTCCGCCTCG
ATGCCCTGAACTTCCTGATGCATGACCCGACCTTGCGGGACAATCCGCCGGCGCCGGACGATGGCCGACGCAAGACGCGG
CCATTCGACTTCCAGCTCAAGATCTACAACCAGTCGCACCCCGACATCCTGAAGTTCATCCAGCGGGTGCGGAACCTGTG
CGACGACTATGGCGCGGTGTTCACAGTCGCGGAAGTCGGCGGAGACCTTGCCGAGACGGAGATGAAGGCGTTCACTGCGG
GGGACAGGCATCTCAACAGCGCCTACGGCTTCGACTTCCTCTACGCGGACAGGCTGACGCCGCATTTCGTGGAAAAGGCC
GTGGCCAAATGGCCCGATGCACCCGGCATGGGCTGGCCGAGCTGGGCCTTCGAGAACCACGATGCGCCGCGCGCGCTTTC
GCGCTGGTGCGCGCCGGACCAGCGCGAGCCGTTCGCCCGGCTGAAGGCCATGCTCTTCGCTTCGTTGCGGGGGAACATCA
TCGTCTACCAGGGCGAGGAACTGGGACTGACGCAGGTCGACATTCCGTTCGAGCAGTTGCAGGACCCCGAGGCCATCGCC
AATTGGCCGCTGACGCTTTCACGCGACGGTGCACGCACGCCGATGCCCTGGTTAGTACAATCGGGCGAGGGCGGGTTCAC
GTCGGGCGCGCCCTGGTTGCCGCTGGGCGAGGAAAACCTGTCGCGGGCCGTCGACCGGCAGGAAGGCGATCCGGCATCGC
TCTTGAACCTGACCACGCGCCTGCTTCGCCTGCGCCGCGAGACGCCCGCGTTACGGATCGGTTCCTTCGAGGTGATCCAT
GCAGACGAATGCCTTCTTGCCATTCGGCGCGTTTTGGGTGAGCAATCGATTGCAGGGCTGTTCAACCTGTCCTCCGTCCC
GGTGGTCTGGCCCCACGGGCTGGTGCGGGAGGGCAAGGAAATGGCGTCGGTCAACGGCGCGACAGTGGGGCAGTTGCCGC
CGTTCGGCGCGCTCCTGATCGAAGAGAGGATCTGA

Upstream 100 bases:

>100_bases
GTCCGTTATCGGTGGCGGCGCCGGGTTCGGTGCTGCTCGATCTCCCCGCATTTGGCAGCGTCGTCTGTCGTGTCATCCTC
TCCCCGGAATCCAGCGTTCG

Downstream 100 bases:

>100_bases
TGCGTAACCTTCTGAACGTTGCAGCGCCGCTGGCGCTGACCCTTTCGTGCGCCCTGGCGACGCCTGCCACGGCGGAGACG
GTGACGGCAACATCGCCGGA

Product: alpha amylase catalytic subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 544; Mature: 544

Protein sequence:

>544_residues
MKQTETAIADPKMHTETPWWRGAAIYQIYPRSFCDSNGDGIGDLNGIASRLDHVARLGVDAIWISPFFTSPMKDFGYDVA
DYCDVDPIFGTLADFDALVKRAHELGLKVTIDQVYAHTSDIHPWFAESRQDRTNARADWYVWADPKPDGSPPSNWQSVFG
GPAWTWDARRCQYYLHNFLSSQPQVNAHNPEVQEALLGAMKFWLDRGVDGFRLDALNFLMHDPTLRDNPPAPDDGRRKTR
PFDFQLKIYNQSHPDILKFIQRVRNLCDDYGAVFTVAEVGGDLAETEMKAFTAGDRHLNSAYGFDFLYADRLTPHFVEKA
VAKWPDAPGMGWPSWAFENHDAPRALSRWCAPDQREPFARLKAMLFASLRGNIIVYQGEELGLTQVDIPFEQLQDPEAIA
NWPLTLSRDGARTPMPWLVQSGEGGFTSGAPWLPLGEENLSRAVDRQEGDPASLLNLTTRLLRLRRETPALRIGSFEVIH
ADECLLAIRRVLGEQSIAGLFNLSSVPVVWPHGLVREGKEMASVNGATVGQLPPFGALLIEERI

Sequences:

>Translated_544_residues
MKQTETAIADPKMHTETPWWRGAAIYQIYPRSFCDSNGDGIGDLNGIASRLDHVARLGVDAIWISPFFTSPMKDFGYDVA
DYCDVDPIFGTLADFDALVKRAHELGLKVTIDQVYAHTSDIHPWFAESRQDRTNARADWYVWADPKPDGSPPSNWQSVFG
GPAWTWDARRCQYYLHNFLSSQPQVNAHNPEVQEALLGAMKFWLDRGVDGFRLDALNFLMHDPTLRDNPPAPDDGRRKTR
PFDFQLKIYNQSHPDILKFIQRVRNLCDDYGAVFTVAEVGGDLAETEMKAFTAGDRHLNSAYGFDFLYADRLTPHFVEKA
VAKWPDAPGMGWPSWAFENHDAPRALSRWCAPDQREPFARLKAMLFASLRGNIIVYQGEELGLTQVDIPFEQLQDPEAIA
NWPLTLSRDGARTPMPWLVQSGEGGFTSGAPWLPLGEENLSRAVDRQEGDPASLLNLTTRLLRLRRETPALRIGSFEVIH
ADECLLAIRRVLGEQSIAGLFNLSSVPVVWPHGLVREGKEMASVNGATVGQLPPFGALLIEERI
>Mature_544_residues
MKQTETAIADPKMHTETPWWRGAAIYQIYPRSFCDSNGDGIGDLNGIASRLDHVARLGVDAIWISPFFTSPMKDFGYDVA
DYCDVDPIFGTLADFDALVKRAHELGLKVTIDQVYAHTSDIHPWFAESRQDRTNARADWYVWADPKPDGSPPSNWQSVFG
GPAWTWDARRCQYYLHNFLSSQPQVNAHNPEVQEALLGAMKFWLDRGVDGFRLDALNFLMHDPTLRDNPPAPDDGRRKTR
PFDFQLKIYNQSHPDILKFIQRVRNLCDDYGAVFTVAEVGGDLAETEMKAFTAGDRHLNSAYGFDFLYADRLTPHFVEKA
VAKWPDAPGMGWPSWAFENHDAPRALSRWCAPDQREPFARLKAMLFASLRGNIIVYQGEELGLTQVDIPFEQLQDPEAIA
NWPLTLSRDGARTPMPWLVQSGEGGFTSGAPWLPLGEENLSRAVDRQEGDPASLLNLTTRLLRLRRETPALRIGSFEVIH
ADECLLAIRRVLGEQSIAGLFNLSSVPVVWPHGLVREGKEMASVNGATVGQLPPFGALLIEERI

Specific function: Unknown

COG id: COG0366

COG function: function code G; Glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Homo sapiens, GI187423904, Length=521, Percent_Identity=32.0537428023033, Blast_Score=253, Evalue=4e-67,
Organism=Escherichia coli, GI1790687, Length=537, Percent_Identity=33.3333333333333, Blast_Score=284, Evalue=9e-78,
Organism=Escherichia coli, GI1786604, Length=490, Percent_Identity=22.0408163265306, Blast_Score=79, Evalue=9e-16,
Organism=Caenorhabditis elegans, GI32565753, Length=488, Percent_Identity=26.2295081967213, Blast_Score=120, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI25147709, Length=473, Percent_Identity=26.8498942917548, Blast_Score=112, Evalue=5e-25,
Organism=Saccharomyces cerevisiae, GI6321726, Length=549, Percent_Identity=34.0619307832423, Blast_Score=286, Evalue=8e-78,
Organism=Saccharomyces cerevisiae, GI6322241, Length=553, Percent_Identity=34.1772151898734, Blast_Score=286, Evalue=8e-78,
Organism=Saccharomyces cerevisiae, GI6322021, Length=553, Percent_Identity=34.1772151898734, Blast_Score=286, Evalue=8e-78,
Organism=Saccharomyces cerevisiae, GI6324416, Length=550, Percent_Identity=34.3636363636364, Blast_Score=285, Evalue=9e-78,
Organism=Saccharomyces cerevisiae, GI6321731, Length=572, Percent_Identity=33.5664335664336, Blast_Score=276, Evalue=7e-75,
Organism=Saccharomyces cerevisiae, GI6319776, Length=572, Percent_Identity=33.5664335664336, Blast_Score=276, Evalue=7e-75,
Organism=Saccharomyces cerevisiae, GI6322245, Length=558, Percent_Identity=31.7204301075269, Blast_Score=256, Evalue=6e-69,
Organism=Drosophila melanogaster, GI24586591, Length=513, Percent_Identity=36.8421052631579, Blast_Score=297, Evalue=1e-80,
Organism=Drosophila melanogaster, GI24583745, Length=510, Percent_Identity=36.8627450980392, Blast_Score=295, Evalue=8e-80,
Organism=Drosophila melanogaster, GI24586593, Length=503, Percent_Identity=36.9781312127237, Blast_Score=294, Evalue=8e-80,
Organism=Drosophila melanogaster, GI24586597, Length=514, Percent_Identity=36.9649805447471, Blast_Score=294, Evalue=1e-79,
Organism=Drosophila melanogaster, GI24586599, Length=529, Percent_Identity=36.1058601134215, Blast_Score=293, Evalue=2e-79,
Organism=Drosophila melanogaster, GI24586589, Length=516, Percent_Identity=36.6279069767442, Blast_Score=289, Evalue=3e-78,
Organism=Drosophila melanogaster, GI24583749, Length=507, Percent_Identity=35.8974358974359, Blast_Score=289, Evalue=4e-78,
Organism=Drosophila melanogaster, GI24583747, Length=507, Percent_Identity=35.8974358974359, Blast_Score=289, Evalue=4e-78,
Organism=Drosophila melanogaster, GI24586587, Length=499, Percent_Identity=36.6733466933868, Blast_Score=286, Evalue=2e-77,
Organism=Drosophila melanogaster, GI221330053, Length=581, Percent_Identity=33.3907056798623, Blast_Score=283, Evalue=2e-76,
Organism=Drosophila melanogaster, GI45549022, Length=521, Percent_Identity=34.5489443378119, Blast_Score=283, Evalue=3e-76,
Organism=Drosophila melanogaster, GI281360393, Length=450, Percent_Identity=33.3333333333333, Blast_Score=214, Evalue=2e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR006589
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF00128 Alpha-amylase [H]

EC number: =3.2.1.20 [H]

Molecular weight: Translated: 61088; Mature: 61088

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQTETAIADPKMHTETPWWRGAAIYQIYPRSFCDSNGDGIGDLNGIASRLDHVARLGVD
CCCCCCCCCCCCCCCCCCCCCCEEEEEECCHHHCCCCCCCCCHHHHHHHHHHHHHHHCCC
AIWISPFFTSPMKDFGYDVADYCDVDPIFGTLADFDALVKRAHELGLKVTIDQVYAHTSD
EEEECCHHCCCHHHHCCCHHHHCCCCHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHCCC
IHPWFAESRQDRTNARADWYVWADPKPDGSPPSNWQSVFGGPAWTWDARRCQYYLHNFLS
CCCHHHHCHHHCCCCCCCEEEECCCCCCCCCCCCHHHHCCCCCCCCHHHHHHHHHHHHHC
SQPQVNAHNPEVQEALLGAMKFWLDRGVDGFRLDALNFLMHDPTLRDNPPAPDDGRRKTR
CCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHCCCCCCCCCCCCCCCCCCCC
PFDFQLKIYNQSHPDILKFIQRVRNLCDDYGAVFTVAEVGGDLAETEMKAFTAGDRHLNS
CCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHEEEHHHHCCCHHHHHHHHHHCCCHHCCH
AYGFDFLYADRLTPHFVEKAVAKWPDAPGMGWPSWAFENHDAPRALSRWCAPDQREPFAR
HCCCCEEECCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCHHHH
LKAMLFASLRGNIIVYQGEELGLTQVDIPFEQLQDPEAIANWPLTLSRDGARTPMPWLVQ
HHHHHHHHHCCCEEEEECCCCCCEEECCCHHHCCCCHHHHCCCEEECCCCCCCCCCCEEE
SGEGGFTSGAPWLPLGEENLSRAVDRQEGDPASLLNLTTRLLRLRRETPALRIGSFEVIH
CCCCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCEEECCEEEEE
ADECLLAIRRVLGEQSIAGLFNLSSVPVVWPHGLVREGKEMASVNGATVGQLPPFGALLI
HHHHHHHHHHHHCHHHHHHHHCCCCCCEECCCHHHHCCHHHHHCCCCCCCCCCCCCHHHE
EERI
ECCC
>Mature Secondary Structure
MKQTETAIADPKMHTETPWWRGAAIYQIYPRSFCDSNGDGIGDLNGIASRLDHVARLGVD
CCCCCCCCCCCCCCCCCCCCCCEEEEEECCHHHCCCCCCCCCHHHHHHHHHHHHHHHCCC
AIWISPFFTSPMKDFGYDVADYCDVDPIFGTLADFDALVKRAHELGLKVTIDQVYAHTSD
EEEECCHHCCCHHHHCCCHHHHCCCCHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHCCC
IHPWFAESRQDRTNARADWYVWADPKPDGSPPSNWQSVFGGPAWTWDARRCQYYLHNFLS
CCCHHHHCHHHCCCCCCCEEEECCCCCCCCCCCCHHHHCCCCCCCCHHHHHHHHHHHHHC
SQPQVNAHNPEVQEALLGAMKFWLDRGVDGFRLDALNFLMHDPTLRDNPPAPDDGRRKTR
CCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHCCCCCCCCCCCCCCCCCCCC
PFDFQLKIYNQSHPDILKFIQRVRNLCDDYGAVFTVAEVGGDLAETEMKAFTAGDRHLNS
CCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHEEEHHHHCCCHHHHHHHHHHCCCHHCCH
AYGFDFLYADRLTPHFVEKAVAKWPDAPGMGWPSWAFENHDAPRALSRWCAPDQREPFAR
HCCCCEEECCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCHHHH
LKAMLFASLRGNIIVYQGEELGLTQVDIPFEQLQDPEAIANWPLTLSRDGARTPMPWLVQ
HHHHHHHHHCCCEEEEECCCCCCEEECCCHHHCCCCHHHHCCCEEECCCCCCCCCCCEEE
SGEGGFTSGAPWLPLGEENLSRAVDRQEGDPASLLNLTTRLLRLRRETPALRIGSFEVIH
CCCCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCEEECCEEEEE
ADECLLAIRRVLGEQSIAGLFNLSSVPVVWPHGLVREGKEMASVNGATVGQLPPFGALLI
HHHHHHHHHHHHCHHHHHHHHCCCCCCEECCCHHHHCCHHHHHCCCCCCCCCCCCCHHHE
EERI
ECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10400573; 11481430 [H]