Click here to switch to the map view.

The map label for this gene is pntB [H]

Identifier: 87199862

GI number: 87199862

Start: 1944995

End: 1946407

Strand: Direct

Name: pntB [H]

Synonym: Saro_1845

Alternate gene names: 87199862

Gene position: 1944995-1946407 (Clockwise)

Preceding gene: 87199861

Following gene: 87199863

Centisome position: 54.61

GC content: 64.54

Gene sequence:

>1413_bases
ATGGAACACGTCGCTGTCTCTCCCTGGGTCGCACTGGCCTATCTCGTCTCGGGCGTCCTGTTCATCCTCGCGCTGCGCGG
GCTTTCCAGCCCCGCGACGAGCCGCGCGGGCAACCGCTATGGCATGATCGGCATGACCATCGCGGTCGCCACGACGCTGC
TCACGCACGAGATCGCCAGTCTGCCGGAAATCCTCGTGGCGCTGGCTATCGGTGGTGGCATTGGCTTCGTCATTGCGCGC
AAGATCGCGATGACCGACATGCCGCAGCTGGTTGCCGCGTTTCACTCGCTGGTCGGTCTGGCTGCGGTGCTGGTGGGCCT
TGCGGCCTACCTGAACCCCGGCGCCTTCGGGATCATGCTGCCGGACGGTCAGATCAACCCGGTCAGCCGCATCGAGCTGG
GCCTCGGCATCGCCATCGGTGCGATCACGTTCTCGGGCTCGGTCATCGCGTTCCTCAAGCTTGCCGGCAAGATGAGCGGA
TCGCCGATCCTGCTGCCGGGACGCCATGTGCTGAACCTCGGCACGCTGATCGCGATCGTCGGGCTGGTGGCATACTTCAC
GCAGGACCAGTCGGCCTGGGTGATCGCGACGATCACGGTGCTCTCGTTCATCATCGGCTTCCTGCTGATCATCCCGATCG
GCGGCGCGGACATGCCGGTGGTCGTCTCGATGCTCAACTCGTACTCGGGCTGGGCAGCGGCGGCGATGGGCTTCACGCTG
CACAACACCGCGATGATCGTGACCGGCGCTCTGGTCGGCTCGTCGGGTGCGATCCTCTCGTACATCATGTGCAAGGCGAT
GAATCGCGGGTTCCTCGCCGTTATCGCGGGCGGCTTCGGTGCCGAAGCTGCTGCCGGTGGCGGCGCCGCGAAGGAACAGC
GCCCGTGGAAGCGCGGGTCCGCCGAAGACGCGGCCTACATGATGAAGGAAGCCGAGAACGTCATCATCATCCCGGGCTAC
GGCATGGCCGTGTCCCAGGCGCAGCACGCGCTGCGCGAGATGGGCGACGTGCTCAAGAAGGAAGGCGTCAACGTCAAATA
CGCCATCCACCCGGTCGCGGGCCGCATGCCCGGCCACATGAACGTGCTGCTGGCCGAAGCCAACGTGCCCTACGATGAAG
TGTTCGAGCTGGAGGACATCAACAGCGAGTTCGCGCAGGCCGACGTGGCCTTCATCATCGGCGCCAACGACGTGGTCAAT
CCGGCCGCGAAGACCGACAAGTCCTCGCCGATCTACGGCATGCCGGTGTTCGACGTCGACAAGGCCAAGACGATCTTCTT
CGTCAAGCGCTCGATGGGCGGCGTGGGCTATGCTGGCGTTGACAACGACGTGTTCTACATGGACCAGACGATGATGCTCC
TGGCCGACGCCAAGAAGATGGTCGAGGACATCGTCAAGGCGCTCCAGCACTGA

Upstream 100 bases:

>100_bases
TTGGGCTGGGTGCGGTCGTTCTGGCAAGCGTCAACATCTTCGGCGGGTTCGCCGTGACCGCGCGCATGCTGGCGATGTAC
AAGAAGAAGGAGAAGAAGTG

Downstream 100 bases:

>100_bases
TGAAAGCGCGCATCGCTCCCGCCGCTTTGGCCCTGGCGCTGGCGGCGGGAGCATGCTCGCCTTCGAAACTGGCTTATGGA
AAGGTCCGCTCGGCGCTGGT

Product: NAD(P) transhydrogenase subunit beta

Products: NA

Alternate protein names: Nicotinamide nucleotide transhydrogenase subunit beta; Proton-translocating transhydrogenase NADP(H)-binding component; Pyridine nucleotide transhydrogenase subunit beta; dIII [H]

Number of amino acids: Translated: 470; Mature: 470

Protein sequence:

>470_residues
MEHVAVSPWVALAYLVSGVLFILALRGLSSPATSRAGNRYGMIGMTIAVATTLLTHEIASLPEILVALAIGGGIGFVIAR
KIAMTDMPQLVAAFHSLVGLAAVLVGLAAYLNPGAFGIMLPDGQINPVSRIELGLGIAIGAITFSGSVIAFLKLAGKMSG
SPILLPGRHVLNLGTLIAIVGLVAYFTQDQSAWVIATITVLSFIIGFLLIIPIGGADMPVVVSMLNSYSGWAAAAMGFTL
HNTAMIVTGALVGSSGAILSYIMCKAMNRGFLAVIAGGFGAEAAAGGGAAKEQRPWKRGSAEDAAYMMKEAENVIIIPGY
GMAVSQAQHALREMGDVLKKEGVNVKYAIHPVAGRMPGHMNVLLAEANVPYDEVFELEDINSEFAQADVAFIIGANDVVN
PAAKTDKSSPIYGMPVFDVDKAKTIFFVKRSMGGVGYAGVDNDVFYMDQTMMLLADAKKMVEDIVKALQH

Sequences:

>Translated_470_residues
MEHVAVSPWVALAYLVSGVLFILALRGLSSPATSRAGNRYGMIGMTIAVATTLLTHEIASLPEILVALAIGGGIGFVIAR
KIAMTDMPQLVAAFHSLVGLAAVLVGLAAYLNPGAFGIMLPDGQINPVSRIELGLGIAIGAITFSGSVIAFLKLAGKMSG
SPILLPGRHVLNLGTLIAIVGLVAYFTQDQSAWVIATITVLSFIIGFLLIIPIGGADMPVVVSMLNSYSGWAAAAMGFTL
HNTAMIVTGALVGSSGAILSYIMCKAMNRGFLAVIAGGFGAEAAAGGGAAKEQRPWKRGSAEDAAYMMKEAENVIIIPGY
GMAVSQAQHALREMGDVLKKEGVNVKYAIHPVAGRMPGHMNVLLAEANVPYDEVFELEDINSEFAQADVAFIIGANDVVN
PAAKTDKSSPIYGMPVFDVDKAKTIFFVKRSMGGVGYAGVDNDVFYMDQTMMLLADAKKMVEDIVKALQH
>Mature_470_residues
MEHVAVSPWVALAYLVSGVLFILALRGLSSPATSRAGNRYGMIGMTIAVATTLLTHEIASLPEILVALAIGGGIGFVIAR
KIAMTDMPQLVAAFHSLVGLAAVLVGLAAYLNPGAFGIMLPDGQINPVSRIELGLGIAIGAITFSGSVIAFLKLAGKMSG
SPILLPGRHVLNLGTLIAIVGLVAYFTQDQSAWVIATITVLSFIIGFLLIIPIGGADMPVVVSMLNSYSGWAAAAMGFTL
HNTAMIVTGALVGSSGAILSYIMCKAMNRGFLAVIAGGFGAEAAAGGGAAKEQRPWKRGSAEDAAYMMKEAENVIIIPGY
GMAVSQAQHALREMGDVLKKEGVNVKYAIHPVAGRMPGHMNVLLAEANVPYDEVFELEDINSEFAQADVAFIIGANDVVN
PAAKTDKSSPIYGMPVFDVDKAKTIFFVKRSMGGVGYAGVDNDVFYMDQTMMLLADAKKMVEDIVKALQH

Specific function: The transhydrogenation between NADH and NADP is coupled to respiration and ATP hydrolysis and functions as a proton pump across the membrane [H]

COG id: COG1282

COG function: function code C; NAD/NADP transhydrogenase beta subunit

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PNT beta subunit family [H]

Homologues:

Organism=Homo sapiens, GI122939155, Length=461, Percent_Identity=48.3731019522777, Blast_Score=396, Evalue=1e-110,
Organism=Homo sapiens, GI122939153, Length=461, Percent_Identity=48.3731019522777, Blast_Score=396, Evalue=1e-110,
Organism=Escherichia coli, GI1787886, Length=463, Percent_Identity=52.4838012958963, Blast_Score=455, Evalue=1e-129,
Organism=Caenorhabditis elegans, GI17550456, Length=466, Percent_Identity=47.2103004291845, Blast_Score=369, Evalue=1e-102,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012136
- InterPro:   IPR004003 [H]

Pfam domain/function: PF02233 PNTB [H]

EC number: =1.6.1.2 [H]

Molecular weight: Translated: 49206; Mature: 49206

Theoretical pI: Translated: 6.43; Mature: 6.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
5.3 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
5.3 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEHVAVSPWVALAYLVSGVLFILALRGLSSPATSRAGNRYGMIGMTIAVATTLLTHEIAS
CCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCCCHHHHHHHHHHHHHHHHHHH
LPEILVALAIGGGIGFVIARKIAMTDMPQLVAAFHSLVGLAAVLVGLAAYLNPGAFGIML
HHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEE
PDGQINPVSRIELGLGIAIGAITFSGSVIAFLKLAGKMSGSPILLPGRHVLNLGTLIAIV
CCCCCCCHHHHHHHHCEEEEEEEECCHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHH
GLVAYFTQDQSAWVIATITVLSFIIGFLLIIPIGGADMPVVVSMLNSYSGWAAAAMGFTL
HHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHEE
HNTAMIVTGALVGSSGAILSYIMCKAMNRGFLAVIAGGFGAEAAAGGGAAKEQRPWKRGS
ECCEEEEEEHHCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCC
AEDAAYMMKEAENVIIIPGYGMAVSQAQHALREMGDVLKKEGVNVKYAIHPVAGRMPGHM
CHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCE
NVLLAEANVPYDEVFELEDINSEFAQADVAFIIGANDVVNPAAKTDKSSPIYGMPVFDVD
EEEEEECCCCHHHHHHHHHCCHHHHHCCEEEEECCCHHCCCCCCCCCCCCEECCCCEECC
KAKTIFFVKRSMGGVGYAGVDNDVFYMDQTMMLLADAKKMVEDIVKALQH
CCCEEEEEEECCCCCEECCCCCCEEEECHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MEHVAVSPWVALAYLVSGVLFILALRGLSSPATSRAGNRYGMIGMTIAVATTLLTHEIAS
CCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCCCHHHHHHHHHHHHHHHHHHH
LPEILVALAIGGGIGFVIARKIAMTDMPQLVAAFHSLVGLAAVLVGLAAYLNPGAFGIML
HHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEE
PDGQINPVSRIELGLGIAIGAITFSGSVIAFLKLAGKMSGSPILLPGRHVLNLGTLIAIV
CCCCCCCHHHHHHHHCEEEEEEEECCHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHH
GLVAYFTQDQSAWVIATITVLSFIIGFLLIIPIGGADMPVVVSMLNSYSGWAAAAMGFTL
HHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHEE
HNTAMIVTGALVGSSGAILSYIMCKAMNRGFLAVIAGGFGAEAAAGGGAAKEQRPWKRGS
ECCEEEEEEHHCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCC
AEDAAYMMKEAENVIIIPGYGMAVSQAQHALREMGDVLKKEGVNVKYAIHPVAGRMPGHM
CHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCE
NVLLAEANVPYDEVFELEDINSEFAQADVAFIIGANDVVNPAAKTDKSSPIYGMPVFDVD
EEEEEECCCCHHHHHHHHHCCHHHHHCCEEEEECCCHHCCCCCCCCCCCCEECCCCEECC
KAKTIFFVKRSMGGVGYAGVDNDVFYMDQTMMLLADAKKMVEDIVKALQH
CCCEEEEEEECCCCCEECCCCCCEEEECHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8075801 [H]