Click here to switch to the map view.

The map label for this gene is ligA

Identifier: 87199755

GI number: 87199755

Start: 1832438

End: 1834603

Strand: Direct

Name: ligA

Synonym: Saro_1738

Alternate gene names: 87199755

Gene position: 1832438-1834603 (Clockwise)

Preceding gene: 87199754

Following gene: 87199756

Centisome position: 51.45

GC content: 65.97

Gene sequence:

>2166_bases
ATGACCACATCGGCCCTCACCGAAGCCGAAGCCGCCAACGAGCTGATGCGCCTGGCAAGGCAGATCGCGAAGCACAACCG
CCTCTATCATGCAGAGGATTCGCCCGAGATCACCGATGCGGAATACGATGCGCTTGTTCGCCGCAATGCTGAACTCGAAG
CTGCCTTTCCGCACCTGATCCGGCCCGACAGCCCGAGCGCGCAGATCGGGCACGAGATTGCCGCTTCGCCCCTGGGCAAG
GTGCAGCACGAGGTTCGCATGATGAGCCTCGACAATGCCTTCACCGACGAGGAGGTCGAGGAATTCGTCGCGCGCGTCCG
CCGTTTCCTGGCGCTGCCCGAAGATGCCGAAGTGGTGATGACCGCCGAAGACAAGATCGACGGCTTGTCCTGCTCGCTGC
GCTACGAGAACGGCAGGCTGGTCCGCGCCGCGACGCGCGGCGATGGACAGGTGGGCGAGGACGTGACCGCCAACGTCGCC
CACATCCCGGACATCCCGCAGGAGTTGAAAGCCGCCGGGCTGTTCGACATCCCCGCCGTCTTCGAGATTCGCGGCGAGGT
CTACATGGCGAAGGACGATTTCCTCGCCCTCAATGCCCGCCAGGCCGAAGCGGGCGAAAAGATCTTCGCCAACCCGCGCA
ACGGCGCTGCGGGTTCGCTCCGCCAGAAGGACGCCAGCGTCACCGCAAGCCGTCCTTTGCGCTTCCTCGCCCATGGCTGG
GGCGCGGCGAGCGAAGTCCCCGCTGCCACCCAGTTCGAGATGATGCGCAAGATTGCGGACTGGGGCGTACCTGTCTCGCC
GCTGCTCGTGCGCTGTTCGTCCGCCGCCGAAATGGTCGCGCACTATCGCGACATTGGCGAGAAGCGCGCTTCGCTGCCCT
ATGACATCGACGGCGTGGTCTACAAGGTCGACCGGCTCGACTGGCAGGACCGGCTCGGCTTCGTCGCGAAGGCCCCGCGC
TGGGGCATTGCCCACAAGTTCCCGGCGGAACGTGCCGAAACCACGCTCGATGCCATCGACATCCAGGTCGGCCGCACCGG
AAAGCTGACGCCGGTCGGCCGCCTCAAGCCGGTACTGGTCGGCGGGGTTACCGTCACCAACGTCACCTTGCACAATCGCG
ATGAAATCGGCCGTCTGGGCCTTCGCGTGGGCGACCGCATCGTCCTCCAGCGGGCAGGCGACGTGATCCCGCAGGTTGTC
GAGAACCTCACCCGCGAAGAACCCCGCGACCCTTACCACTTTCCCGACCATTGCCCCGAATGCGGGTCCGAAGCCGTTGC
CGAGGAAGGCGAGGTCGACGTGCGCTGCACCGGCGGCCTGATCTGCCCGGCCCAGCGCGTCGAACGCCTCAAGCACTTCG
TCAGCCGCGCCGCGCTCGACATCGAAGGGCTGGGCGAAAAGACAATCATCGAATTCTTCCAGCTGGGCTGGCTCGAAAGC
CCCGCCGATATCTTCCGCCTCAGGAAGCGCCGCAGCGAGATCGTCGGCCGTGAAGGCTGGAAGGACAAGTCGGTCGACAA
CCTTCTCGCCGCGATCGAGGCCAAGCGCCAGCCCGATGCCGCCCGCCTGCTGTTCGGCCTTGGCATCCGGCATGTCGGTG
CGGTCACCGCCCGGGACCTTATGAAACGCTTCGTCACGCTTCCCGCTCTGCGCGAAGCCGCCCGGCAAGCATCGTCGGCG
GCACGGGAAGGGGAACCGGCGAACGCCGATGGAGCGTACGATCCGGCAACAGTTACGCCCGATTCCGACACTGCCGGCGC
GGAGGCGGGCCGATCCGATGCCTTGGCCGACCTTCTGTCCATCGATGGCGTCGGCCCGGTCGTGGTCGAGGCGCTAGGCG
ATTTCTTCCACGAACCCCACAACATCGCCGTCTGGGAAGATCTGCTTTCCGAAGTCTCGCCGCCGCCCTATGTCGTCGAA
ACGAAGGACAGTGCCGTGGCCGGAAAGACCATCGTGTTCACCGGCAAGCTCGAAACCATGAGCCGTGACGAAGCCAAGGC
ACAGGCCGAGGCCTTGGGCGCGCGGACGGCGGGCTCGGTTTCGGCCAAGACCGATCTGGTCGTGGCGGGGCCGGGCGCAG
GTTCCAAGCTGAAGCAGGCGGCGGCGCTGGGCATCGATGTGATCGACGAGGCGGCATGGGCCGAAATCGTCAGGCAGGCG
GGGTAA

Upstream 100 bases:

>100_bases
TGCACAGTTACGCCAGCAACGCCTCGGCAATCCGCCTCTACGAATCCCTCGGCTTCCGCGCCCGCCGCGCGATGACGGCC
ACGCTTCTCGGGAAATCGAC

Downstream 100 bases:

>100_bases
GCGCAGTGGCATTCCATACCTGGTGGCTGTTCGTCGTCACCGTGTTCTTCATCTCGGGATCGCCCGGCCCCAACATGCTG
CACGTGATGACGCGCAGCGT

Product: DNA ligase, NAD-dependent

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+]

Number of amino acids: Translated: 721; Mature: 720

Protein sequence:

>721_residues
MTTSALTEAEAANELMRLARQIAKHNRLYHAEDSPEITDAEYDALVRRNAELEAAFPHLIRPDSPSAQIGHEIAASPLGK
VQHEVRMMSLDNAFTDEEVEEFVARVRRFLALPEDAEVVMTAEDKIDGLSCSLRYENGRLVRAATRGDGQVGEDVTANVA
HIPDIPQELKAAGLFDIPAVFEIRGEVYMAKDDFLALNARQAEAGEKIFANPRNGAAGSLRQKDASVTASRPLRFLAHGW
GAASEVPAATQFEMMRKIADWGVPVSPLLVRCSSAAEMVAHYRDIGEKRASLPYDIDGVVYKVDRLDWQDRLGFVAKAPR
WGIAHKFPAERAETTLDAIDIQVGRTGKLTPVGRLKPVLVGGVTVTNVTLHNRDEIGRLGLRVGDRIVLQRAGDVIPQVV
ENLTREEPRDPYHFPDHCPECGSEAVAEEGEVDVRCTGGLICPAQRVERLKHFVSRAALDIEGLGEKTIIEFFQLGWLES
PADIFRLRKRRSEIVGREGWKDKSVDNLLAAIEAKRQPDAARLLFGLGIRHVGAVTARDLMKRFVTLPALREAARQASSA
AREGEPANADGAYDPATVTPDSDTAGAEAGRSDALADLLSIDGVGPVVVEALGDFFHEPHNIAVWEDLLSEVSPPPYVVE
TKDSAVAGKTIVFTGKLETMSRDEAKAQAEALGARTAGSVSAKTDLVVAGPGAGSKLKQAAALGIDVIDEAAWAEIVRQA
G

Sequences:

>Translated_721_residues
MTTSALTEAEAANELMRLARQIAKHNRLYHAEDSPEITDAEYDALVRRNAELEAAFPHLIRPDSPSAQIGHEIAASPLGK
VQHEVRMMSLDNAFTDEEVEEFVARVRRFLALPEDAEVVMTAEDKIDGLSCSLRYENGRLVRAATRGDGQVGEDVTANVA
HIPDIPQELKAAGLFDIPAVFEIRGEVYMAKDDFLALNARQAEAGEKIFANPRNGAAGSLRQKDASVTASRPLRFLAHGW
GAASEVPAATQFEMMRKIADWGVPVSPLLVRCSSAAEMVAHYRDIGEKRASLPYDIDGVVYKVDRLDWQDRLGFVAKAPR
WGIAHKFPAERAETTLDAIDIQVGRTGKLTPVGRLKPVLVGGVTVTNVTLHNRDEIGRLGLRVGDRIVLQRAGDVIPQVV
ENLTREEPRDPYHFPDHCPECGSEAVAEEGEVDVRCTGGLICPAQRVERLKHFVSRAALDIEGLGEKTIIEFFQLGWLES
PADIFRLRKRRSEIVGREGWKDKSVDNLLAAIEAKRQPDAARLLFGLGIRHVGAVTARDLMKRFVTLPALREAARQASSA
AREGEPANADGAYDPATVTPDSDTAGAEAGRSDALADLLSIDGVGPVVVEALGDFFHEPHNIAVWEDLLSEVSPPPYVVE
TKDSAVAGKTIVFTGKLETMSRDEAKAQAEALGARTAGSVSAKTDLVVAGPGAGSKLKQAAALGIDVIDEAAWAEIVRQA
G
>Mature_720_residues
TTSALTEAEAANELMRLARQIAKHNRLYHAEDSPEITDAEYDALVRRNAELEAAFPHLIRPDSPSAQIGHEIAASPLGKV
QHEVRMMSLDNAFTDEEVEEFVARVRRFLALPEDAEVVMTAEDKIDGLSCSLRYENGRLVRAATRGDGQVGEDVTANVAH
IPDIPQELKAAGLFDIPAVFEIRGEVYMAKDDFLALNARQAEAGEKIFANPRNGAAGSLRQKDASVTASRPLRFLAHGWG
AASEVPAATQFEMMRKIADWGVPVSPLLVRCSSAAEMVAHYRDIGEKRASLPYDIDGVVYKVDRLDWQDRLGFVAKAPRW
GIAHKFPAERAETTLDAIDIQVGRTGKLTPVGRLKPVLVGGVTVTNVTLHNRDEIGRLGLRVGDRIVLQRAGDVIPQVVE
NLTREEPRDPYHFPDHCPECGSEAVAEEGEVDVRCTGGLICPAQRVERLKHFVSRAALDIEGLGEKTIIEFFQLGWLESP
ADIFRLRKRRSEIVGREGWKDKSVDNLLAAIEAKRQPDAARLLFGLGIRHVGAVTARDLMKRFVTLPALREAARQASSAA
REGEPANADGAYDPATVTPDSDTAGAEAGRSDALADLLSIDGVGPVVVEALGDFFHEPHNIAVWEDLLSEVSPPPYVVET
KDSAVAGKTIVFTGKLETMSRDEAKAQAEALGARTAGSVSAKTDLVVAGPGAGSKLKQAAALGIDVIDEAAWAEIVRQAG

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain

Homologues:

Organism=Escherichia coli, GI1788750, Length=720, Percent_Identity=45.6944444444444, Blast_Score=563, Evalue=1e-161,
Organism=Escherichia coli, GI87082305, Length=468, Percent_Identity=24.3589743589744, Blast_Score=100, Evalue=3e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DNLJ_NOVAD (Q2G7J5)

Other databases:

- EMBL:   CP000248
- RefSeq:   YP_497012.1
- ProteinModelPortal:   Q2G7J5
- STRING:   Q2G7J5
- GeneID:   3916313
- GenomeReviews:   CP000248_GR
- KEGG:   nar:Saro_1738
- eggNOG:   COG0272
- HOGENOM:   HBG620317
- OMA:   IKHFASR
- PhylomeDB:   Q2G7J5
- ProtClustDB:   CLSK843976
- BioCyc:   NARO279238:SARO_1738-MONOMER
- GO:   GO:0005622
- HAMAP:   MF_01588
- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149
- Gene3D:   G3DSA:2.40.50.140
- PIRSF:   PIRSF001604
- SMART:   SM00292
- SMART:   SM00278
- SMART:   SM00532
- TIGRFAMs:   TIGR00575

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD; SSF52113 BRCT; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like

EC number: =6.5.1.2

Molecular weight: Translated: 78116; Mature: 77985

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: PS50172 BRCT; PS01055 DNA_LIGASE_N1; PS01056 DNA_LIGASE_N2

Important sites: ACT_SITE 125-125 BINDING 123-123 BINDING 146-146 BINDING 186-186 BINDING 302-302 BINDING 326-326

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTSALTEAEAANELMRLARQIAKHNRLYHAEDSPEITDAEYDALVRRNAELEAAFPHLI
CCCHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCHHHHHHHHCCCCHHHCCCHHC
RPDSPSAQIGHEIAASPLGKVQHEVRMMSLDNAFTDEEVEEFVARVRRFLALPEDAEVVM
CCCCCCHHHHHHHHHCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCEEE
TAEDKIDGLSCSLRYENGRLVRAATRGDGQVGEDVTANVAHIPDIPQELKAAGLFDIPAV
EECCCCCCCEEEEEECCCCEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCHH
FEIRGEVYMAKDDFLALNARQAEAGEKIFANPRNGAAGSLRQKDASVTASRPLRFLAHGW
EEECCCEEEECCCEEEEECHHHHCCCCEECCCCCCCCCCCHHHCCCCCHHCCHHHHHHCC
GAASEVPAATQFEMMRKIADWGVPVSPLLVRCSSAAEMVAHYRDIGEKRASLPYDIDGVV
CCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEE
YKVDRLDWQDRLGFVAKAPRWGIAHKFPAERAETTLDAIDIQVGRTGKLTPVGRLKPVLV
EEEECCCHHHHCCHHHCCCCCCCCCCCCHHHHHHHHHHEEEEECCCCCCCCCCCCCCEEE
GGVTVTNVTLHNRDEIGRLGLRVGDRIVLQRAGDVIPQVVENLTREEPRDPYHFPDHCPE
CCEEEEEEEEECCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCH
CGSEAVAEEGEVDVRCTGGLICPAQRVERLKHFVSRAALDIEGLGEKTIIEFFQLGWLES
HCHHHHHCCCCEEEEECCCEECHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCC
PADIFRLRKRRSEIVGREGWKDKSVDNLLAAIEAKRQPDAARLLFGLGIRHVGAVTARDL
HHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
MKRFVTLPALREAARQASSAAREGEPANADGAYDPATVTPDSDTAGAEAGRSDALADLLS
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
IDGVGPVVVEALGDFFHEPHNIAVWEDLLSEVSPPPYVVETKDSAVAGKTIVFTGKLETM
CCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCEEEECCCCCCCCCEEEEECCHHHH
SRDEAKAQAEALGARTAGSVSAKTDLVVAGPGAGSKLKQAAALGIDVIDEAAWAEIVRQA
CHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHCHHHHHHHHHHHHHHHC
G
C
>Mature Secondary Structure 
TTSALTEAEAANELMRLARQIAKHNRLYHAEDSPEITDAEYDALVRRNAELEAAFPHLI
CCHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCHHHHHHHHCCCCHHHCCCHHC
RPDSPSAQIGHEIAASPLGKVQHEVRMMSLDNAFTDEEVEEFVARVRRFLALPEDAEVVM
CCCCCCHHHHHHHHHCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCEEE
TAEDKIDGLSCSLRYENGRLVRAATRGDGQVGEDVTANVAHIPDIPQELKAAGLFDIPAV
EECCCCCCCEEEEEECCCCEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCHH
FEIRGEVYMAKDDFLALNARQAEAGEKIFANPRNGAAGSLRQKDASVTASRPLRFLAHGW
EEECCCEEEECCCEEEEECHHHHCCCCEECCCCCCCCCCCHHHCCCCCHHCCHHHHHHCC
GAASEVPAATQFEMMRKIADWGVPVSPLLVRCSSAAEMVAHYRDIGEKRASLPYDIDGVV
CCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEE
YKVDRLDWQDRLGFVAKAPRWGIAHKFPAERAETTLDAIDIQVGRTGKLTPVGRLKPVLV
EEEECCCHHHHCCHHHCCCCCCCCCCCCHHHHHHHHHHEEEEECCCCCCCCCCCCCCEEE
GGVTVTNVTLHNRDEIGRLGLRVGDRIVLQRAGDVIPQVVENLTREEPRDPYHFPDHCPE
CCEEEEEEEEECCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCH
CGSEAVAEEGEVDVRCTGGLICPAQRVERLKHFVSRAALDIEGLGEKTIIEFFQLGWLES
HCHHHHHCCCCEEEEECCCEECHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCC
PADIFRLRKRRSEIVGREGWKDKSVDNLLAAIEAKRQPDAARLLFGLGIRHVGAVTARDL
HHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
MKRFVTLPALREAARQASSAAREGEPANADGAYDPATVTPDSDTAGAEAGRSDALADLLS
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
IDGVGPVVVEALGDFFHEPHNIAVWEDLLSEVSPPPYVVETKDSAVAGKTIVFTGKLETM
CCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCEEEECCCCCCCCCEEEEECCHHHH
SRDEAKAQAEALGARTAGSVSAKTDLVVAGPGAGSKLKQAAALGIDVIDEAAWAEIVRQA
CHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHCHHHHHHHHHHHHHHHC
G
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA