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The map label for this gene is benA [H]

Identifier: 87199697

GI number: 87199697

Start: 1764996

End: 1766339

Strand: Direct

Name: benA [H]

Synonym: Saro_1680

Alternate gene names: 87199697

Gene position: 1764996-1766339 (Clockwise)

Preceding gene: 87199696

Following gene: 87199698

Centisome position: 49.56

GC content: 64.73

Gene sequence:

>1344_bases
GTGACCTTGCGCCTTTCCGACCTTATCGACGACCGCCCTTCGCAAGGCGTGTTCCGCGTGAACCGCGCGATCTTCACCGA
CCAAAGCGTGTTCGACGCGGAAATGCGCCGCCTGTTCGAGGGCGGATGGGTCTTTCTCGGCATGGAATCGCAGGCGTCCG
GTCCGCACGATTTCTTCACCACCTCCGCCGGGCGCGTGCCGGTCATGGTCCAGCGCGACGGGGAGGGCGTGTTGCGCGCC
TTCGTCAATTCCTGCCCGCACAAGGGCGCGCGCCTGGCGCAAGTGCGGCAAGGCAACGCGCGGCTGCACGTCTGTCCGTA
CCATAGCTGGTCGTTCGACAGCGCCGGCCGCAACAAGGCCGTGAAATGGAAGGCGGCCGGGTGCTATTCCGATGCCTTCG
ACCGCGACGATCATGGCCTCGCGGTCCTCCCGCGCTTTGAAGGCTATCGCGGCTTCCTGTTCGGCAGCGTTTCGCCTGAA
GTCCCGCCGCTGGCCGAACATCTCGGCGAAGCCGCCAAGCTGCTCGATCTCGTCGCGGATCAGAGCGAGGAAGGGCTGGA
ACTCGTGCCCGGGCAGGTGACCTTCACCTATCAGGCGAACTGGAAGCTCCAGCTCGAGAACTGTTCCGACGCCTATCACT
TCACATCCGCCCACCCATCCTACATCCGCGTGCTGGAACGGCGGCAGAAGGAGATCAGCGAAGAGGTCGTTGCCTCGGTG
TGGGAGAACAGCGACTACTGGAAGGAGGACACCAAGGGCGTCGGCGGCGGCAGCTTCAGCATGGCAAATGGCCACGTGCT
GAACTGGGGTGTGTTTGGCGTCACCCCCGCGATCCCGCTCTACGAACGCGCGGCGCAACTGGCGGAGCGTGTGGGTGAGG
GGAAGCGCGACTGGATGTTCAACATGCGCAACCTCACGATCTTTCCCAACCTGCAGGTCGCGGAGAACGCGTCGAGCCAG
CTTCGCGTGATCCGCCCGATTTCGCCGGCGCTTACCGAAATGCGCACCTGGTGCATCGCGCCCAAGGGGGAAAGCGACGC
TGCCCGCCGCCAGCGCATTCGGCAGTACGAGGACTTCTTCAACCCCACCGGCATGGCAACGCCCGACGACACCGTGTCTT
ACGAAAACTGCCAGATCGGCTTTGCCGGCACGACCGAACCCTGGCTCCAGGGGTATGCGCGGGGGATGGAGGCCTCGGTC
GAAGGCGGCAACCGCTTTTCCGAACGTATCGGACTGGAACCGCAGCGCAGCGTTCTGGCGGACTCGCAGCTTTGCGACGA
AACGCTCTACCACAGCTACTACCGCGCCTGGGCCGCGCGCATGGCACCGGAGTTCGCGGCATGA

Upstream 100 bases:

>100_bases
GCATCGTCGCACGCCGCGCCGAGGACGCGGCATCGCTCGACGAGGCGCTGCGCTGGTCGCTCGCCGCCGACGTGCCGACG
CTGGTCGAAGCGGTGGTGCT

Downstream 100 bases:

>100_bases
GCACGCCCCTGTCCCGCGCGGAGGCCGAGGAGCTGCTGTTCCGCGAAGCCGTGCTGATCGACACCGGTCGCTTCGCCGAA
TGGCTCGACCTGTTCACGCC

Product: ring hydroxylating dioxygenase subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 447; Mature: 446

Protein sequence:

>447_residues
MTLRLSDLIDDRPSQGVFRVNRAIFTDQSVFDAEMRRLFEGGWVFLGMESQASGPHDFFTTSAGRVPVMVQRDGEGVLRA
FVNSCPHKGARLAQVRQGNARLHVCPYHSWSFDSAGRNKAVKWKAAGCYSDAFDRDDHGLAVLPRFEGYRGFLFGSVSPE
VPPLAEHLGEAAKLLDLVADQSEEGLELVPGQVTFTYQANWKLQLENCSDAYHFTSAHPSYIRVLERRQKEISEEVVASV
WENSDYWKEDTKGVGGGSFSMANGHVLNWGVFGVTPAIPLYERAAQLAERVGEGKRDWMFNMRNLTIFPNLQVAENASSQ
LRVIRPISPALTEMRTWCIAPKGESDAARRQRIRQYEDFFNPTGMATPDDTVSYENCQIGFAGTTEPWLQGYARGMEASV
EGGNRFSERIGLEPQRSVLADSQLCDETLYHSYYRAWAARMAPEFAA

Sequences:

>Translated_447_residues
MTLRLSDLIDDRPSQGVFRVNRAIFTDQSVFDAEMRRLFEGGWVFLGMESQASGPHDFFTTSAGRVPVMVQRDGEGVLRA
FVNSCPHKGARLAQVRQGNARLHVCPYHSWSFDSAGRNKAVKWKAAGCYSDAFDRDDHGLAVLPRFEGYRGFLFGSVSPE
VPPLAEHLGEAAKLLDLVADQSEEGLELVPGQVTFTYQANWKLQLENCSDAYHFTSAHPSYIRVLERRQKEISEEVVASV
WENSDYWKEDTKGVGGGSFSMANGHVLNWGVFGVTPAIPLYERAAQLAERVGEGKRDWMFNMRNLTIFPNLQVAENASSQ
LRVIRPISPALTEMRTWCIAPKGESDAARRQRIRQYEDFFNPTGMATPDDTVSYENCQIGFAGTTEPWLQGYARGMEASV
EGGNRFSERIGLEPQRSVLADSQLCDETLYHSYYRAWAARMAPEFAA
>Mature_446_residues
TLRLSDLIDDRPSQGVFRVNRAIFTDQSVFDAEMRRLFEGGWVFLGMESQASGPHDFFTTSAGRVPVMVQRDGEGVLRAF
VNSCPHKGARLAQVRQGNARLHVCPYHSWSFDSAGRNKAVKWKAAGCYSDAFDRDDHGLAVLPRFEGYRGFLFGSVSPEV
PPLAEHLGEAAKLLDLVADQSEEGLELVPGQVTFTYQANWKLQLENCSDAYHFTSAHPSYIRVLERRQKEISEEVVASVW
ENSDYWKEDTKGVGGGSFSMANGHVLNWGVFGVTPAIPLYERAAQLAERVGEGKRDWMFNMRNLTIFPNLQVAENASSQL
RVIRPISPALTEMRTWCIAPKGESDAARRQRIRQYEDFFNPTGMATPDDTVSYENCQIGFAGTTEPWLQGYARGMEASVE
GGNRFSERIGLEPQRSVLADSQLCDETLYHSYYRAWAARMAPEFAA

Specific function: Degradation of benzoate to 2-hydro-1,2-dihydroxybenzoate (DHB) [H]

COG id: COG4638

COG function: function code PR; Phenylpropionate dioxygenase and related ring-hydroxylating dioxygenases, large terminal subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Rieske domain [H]

Homologues:

Organism=Escherichia coli, GI1788888, Length=455, Percent_Identity=29.8901098901099, Blast_Score=163, Evalue=3e-41,
Organism=Escherichia coli, GI1788103, Length=199, Percent_Identity=30.1507537688442, Blast_Score=93, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017639
- InterPro:   IPR017941
- InterPro:   IPR015881
- InterPro:   IPR015879
- InterPro:   IPR001663 [H]

Pfam domain/function: PF00355 Rieske; PF00848 Ring_hydroxyl_A [H]

EC number: =1.14.12.10 [H]

Molecular weight: Translated: 50110; Mature: 49978

Theoretical pI: Translated: 5.66; Mature: 5.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLRLSDLIDDRPSQGVFRVNRAIFTDQSVFDAEMRRLFEGGWVFLGMESQASGPHDFFT
CEEECHHHHCCCCCCCCEEEHHHHCCCCHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHC
TSAGRVPVMVQRDGEGVLRAFVNSCPHKGARLAQVRQGNARLHVCPYHSWSFDSAGRNKA
CCCCCCEEEEEECCHHHHHHHHHHCCCCCCHHHHHHCCCCEEEEECCCCCCCCCCCCCCE
VKWKAAGCYSDAFDRDDHGLAVLPRFEGYRGFLFGSVSPEVPPLAEHLGEAAKLLDLVAD
EEEEECCCCCCCCCCCCCCEEEEECCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHC
QSEEGLELVPGQVTFTYQANWKLQLENCSDAYHFTSAHPSYIRVLERRQKEISEEVVASV
CCCCCCEECCCEEEEEEECCEEEEECCCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHH
WENSDYWKEDTKGVGGGSFSMANGHVLNWGVFGVTPAIPLYERAAQLAERVGEGKRDWMF
HCCCCCCHHHCCCCCCCCEECCCCCEEEEEHHCCCCCCHHHHHHHHHHHHHCCCCHHHEE
NMRNLTIFPNLQVAENASSQLRVIRPISPALTEMRTWCIAPKGESDAARRQRIRQYEDFF
EECCEEEECCCHHHCCCCCCEEEEECCCHHHHHHHHEECCCCCCCHHHHHHHHHHHHHHC
NPTGMATPDDTVSYENCQIGFAGTTEPWLQGYARGMEASVEGGNRFSERIGLEPQRSVLA
CCCCCCCCCCCCCCCCEEEECCCCCCHHHHHHHCCCCCCCCCCCHHHHHCCCCHHHHHHH
DSQLCDETLYHSYYRAWAARMAPEFAA
HHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
TLRLSDLIDDRPSQGVFRVNRAIFTDQSVFDAEMRRLFEGGWVFLGMESQASGPHDFFT
EEECHHHHCCCCCCCCEEEHHHHCCCCHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHC
TSAGRVPVMVQRDGEGVLRAFVNSCPHKGARLAQVRQGNARLHVCPYHSWSFDSAGRNKA
CCCCCCEEEEEECCHHHHHHHHHHCCCCCCHHHHHHCCCCEEEEECCCCCCCCCCCCCCE
VKWKAAGCYSDAFDRDDHGLAVLPRFEGYRGFLFGSVSPEVPPLAEHLGEAAKLLDLVAD
EEEEECCCCCCCCCCCCCCEEEEECCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHC
QSEEGLELVPGQVTFTYQANWKLQLENCSDAYHFTSAHPSYIRVLERRQKEISEEVVASV
CCCCCCEECCCEEEEEEECCEEEEECCCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHH
WENSDYWKEDTKGVGGGSFSMANGHVLNWGVFGVTPAIPLYERAAQLAERVGEGKRDWMF
HCCCCCCHHHCCCCCCCCEECCCCCEEEEEHHCCCCCCHHHHHHHHHHHHHCCCCHHHEE
NMRNLTIFPNLQVAENASSQLRVIRPISPALTEMRTWCIAPKGESDAARRQRIRQYEDFF
EECCEEEECCCHHHCCCCCCEEEEECCCHHHHHHHHEECCCCCCCHHHHHHHHHHHHHHC
NPTGMATPDDTVSYENCQIGFAGTTEPWLQGYARGMEASVEGGNRFSERIGLEPQRSVLA
CCCCCCCCCCCCCCCCEEEECCCCCCHHHHHHHCCCCCCCCCCCHHHHHCCCCHHHHHHH
DSQLCDETLYHSYYRAWAARMAPEFAA
HHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1885518 [H]