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The map label for this gene is mdlC [H]

Identifier: 87199696

GI number: 87199696

Start: 1763443

End: 1764999

Strand: Direct

Name: mdlC [H]

Synonym: Saro_1679

Alternate gene names: 87199696

Gene position: 1763443-1764999 (Clockwise)

Preceding gene: 87199693

Following gene: 87199697

Centisome position: 49.51

GC content: 67.31

Gene sequence:

>1557_bases
ATGTCGACCGTCCGTCACGTTACCATCGACCTTCTGCGCGAACTCGGCATGACTACCGTTTTCGGCAATCCGGGCTCCAC
CGAACTTCCGATGTTCCGCGATTTTCCGGACGATTTCCGCTATGTCATGGGCCTTCAGGAAAGCGTCGTGCTGGGCATGG
CCGATGGCTTTGCGCAAGGGACGGGCCGGGCAGCCATCGTCAACCTGCACAGTTCGGCGGGCGTCGGGCACGCGCTCGGC
AACCTCTTTACCGCGTTCAAGAACCAGACTCCGCTAGTGGTGACGGCGGGGCAACAGGCGCGCTCGATCCTGCCTTACGA
GCCCTTCCTCTTCGCCGAACGCGCCAGTGAGTTTCCGCGTCCGTTCGTGAAATGGTCCTGCGAACCGGCCCGGGCGGAAG
ACGTTCCCGCCGCGATCCACCGCGCCTGGCTGGTGGCGATGGAGCCGCCTTGCGGGCCGACCTTCGTTTCGATCCCCATC
GACGACTGGGATCGGTCGTGTGAGCCGTTCGCGGCGCGAAAGGTTCATGCAGACCGGGCTGGCGATCCTGCTGCTCTCGC
CGCGTGCGCGACGTCGATGGCGCAGGCGCGACGTCCTGCCATCGTCGTTGGTGCGGGGGTGGCGCGCGATGGCGCCTGGG
ACCGCGTGATCGCCCTTGCGGAGCGTCATCAGGCGGCGGTCTGGGTGGCGCCGATGTCCGCCCGGTGCAGTTTTCCGGAA
GACCACCCGCTTTTCGCGGGGTTCCTTACGGCCGGGCGCGAGGCCATCGTGGCCACGCTTTCGTCCCACGACTTCGTGCT
TGCGCTGGGCGGGCCGATGAACCTCTACCACGTCGAGGGACATGGGCCGCATATGCCGGAGGGCTGCGATGTCTGGCTGA
TCGGTGACAACCATATCCATGCCGCATGGGCGCCGGCGGGAACTGCCATCGTGGCGCAGTGCGACAGGGCGCTCGATGCC
TTGCTGGCCGGGCCGGAGCCGGTGCAGCGCGATGCTCCACCCGTGCGCCAGCGCCTGCCGCGGCTTGATGGCGCGGCTCT
TACCGATGCCTATGTCCTCCAGCGCCTTGCCGCCCTGCGCAGTCCCGAGAGCATCGTCGTGGAGGAAGCGCCTTCGAGCC
GGGGGCCGATGCACGAACACCTGCCGATCCTGCGCAAGGACACCTTCTATACCACGGCCAGCGGCGGGCTCGGCCACGGG
CTTCCGGCCGCTGTGGGCATGGCGATGGCGCGGCCGGACGACAAGGTCATTGCGCTGCTTGGCGATGGTTCCGCCATGTA
CGCGATCCAGGGGCTCCACGCGGCGGCGCAGCATGGCCTGCCGGTCAGCTTCCTCATCCTCAAGAACAACCGCTATGAGG
CGCTGCACCATTTCGGGCGTCATTTCGGCATGCAGCAGCTCGTCGGCACGCAGTTCCCGGAACTGGACTTCTGCAAGCTT
GCCGAAGGCCACGGCATCGTCGCACGCCGCGCCGAGGACGCGGCATCGCTCGACGAGGCGCTGCGCTGGTCGCTCGCCGC
CGACGTGCCGACGCTGGTCGAAGCGGTGGTGCTGTGA

Upstream 100 bases:

>100_bases
AAATCCATGCGCAAGGCGTATGGCTTTCCGCCAAGATTGCAATTGTTTCTAAGGCTGCGGCAGTTCATCCTCGGTTGACA
AGCATATGCCCGGAGAGATC

Downstream 100 bases:

>100_bases
CCTTGCGCCTTTCCGACCTTATCGACGACCGCCCTTCGCAAGGCGTGTTCCGCGTGAACCGCGCGATCTTCACCGACCAA
AGCGTGTTCGACGCGGAAAT

Product: benzoylformate decarboxylase

Products: NA

Alternate protein names: BFD; BFDC [H]

Number of amino acids: Translated: 518; Mature: 517

Protein sequence:

>518_residues
MSTVRHVTIDLLRELGMTTVFGNPGSTELPMFRDFPDDFRYVMGLQESVVLGMADGFAQGTGRAAIVNLHSSAGVGHALG
NLFTAFKNQTPLVVTAGQQARSILPYEPFLFAERASEFPRPFVKWSCEPARAEDVPAAIHRAWLVAMEPPCGPTFVSIPI
DDWDRSCEPFAARKVHADRAGDPAALAACATSMAQARRPAIVVGAGVARDGAWDRVIALAERHQAAVWVAPMSARCSFPE
DHPLFAGFLTAGREAIVATLSSHDFVLALGGPMNLYHVEGHGPHMPEGCDVWLIGDNHIHAAWAPAGTAIVAQCDRALDA
LLAGPEPVQRDAPPVRQRLPRLDGAALTDAYVLQRLAALRSPESIVVEEAPSSRGPMHEHLPILRKDTFYTTASGGLGHG
LPAAVGMAMARPDDKVIALLGDGSAMYAIQGLHAAAQHGLPVSFLILKNNRYEALHHFGRHFGMQQLVGTQFPELDFCKL
AEGHGIVARRAEDAASLDEALRWSLAADVPTLVEAVVL

Sequences:

>Translated_518_residues
MSTVRHVTIDLLRELGMTTVFGNPGSTELPMFRDFPDDFRYVMGLQESVVLGMADGFAQGTGRAAIVNLHSSAGVGHALG
NLFTAFKNQTPLVVTAGQQARSILPYEPFLFAERASEFPRPFVKWSCEPARAEDVPAAIHRAWLVAMEPPCGPTFVSIPI
DDWDRSCEPFAARKVHADRAGDPAALAACATSMAQARRPAIVVGAGVARDGAWDRVIALAERHQAAVWVAPMSARCSFPE
DHPLFAGFLTAGREAIVATLSSHDFVLALGGPMNLYHVEGHGPHMPEGCDVWLIGDNHIHAAWAPAGTAIVAQCDRALDA
LLAGPEPVQRDAPPVRQRLPRLDGAALTDAYVLQRLAALRSPESIVVEEAPSSRGPMHEHLPILRKDTFYTTASGGLGHG
LPAAVGMAMARPDDKVIALLGDGSAMYAIQGLHAAAQHGLPVSFLILKNNRYEALHHFGRHFGMQQLVGTQFPELDFCKL
AEGHGIVARRAEDAASLDEALRWSLAADVPTLVEAVVL
>Mature_517_residues
STVRHVTIDLLRELGMTTVFGNPGSTELPMFRDFPDDFRYVMGLQESVVLGMADGFAQGTGRAAIVNLHSSAGVGHALGN
LFTAFKNQTPLVVTAGQQARSILPYEPFLFAERASEFPRPFVKWSCEPARAEDVPAAIHRAWLVAMEPPCGPTFVSIPID
DWDRSCEPFAARKVHADRAGDPAALAACATSMAQARRPAIVVGAGVARDGAWDRVIALAERHQAAVWVAPMSARCSFPED
HPLFAGFLTAGREAIVATLSSHDFVLALGGPMNLYHVEGHGPHMPEGCDVWLIGDNHIHAAWAPAGTAIVAQCDRALDAL
LAGPEPVQRDAPPVRQRLPRLDGAALTDAYVLQRLAALRSPESIVVEEAPSSRGPMHEHLPILRKDTFYTTASGGLGHGL
PAAVGMAMARPDDKVIALLGDGSAMYAIQGLHAAAQHGLPVSFLILKNNRYEALHHFGRHFGMQQLVGTQFPELDFCKLA
EGHGIVARRAEDAASLDEALRWSLAADVPTLVEAVVL

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Escherichia coli, GI87081685, Length=528, Percent_Identity=24.8106060606061, Blast_Score=124, Evalue=2e-29,
Organism=Escherichia coli, GI1790104, Length=520, Percent_Identity=24.8076923076923, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI1786717, Length=552, Percent_Identity=24.8188405797101, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI1787096, Length=123, Percent_Identity=30.0813008130081, Blast_Score=63, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6323755, Length=548, Percent_Identity=23.9051094890511, Blast_Score=116, Evalue=1e-26,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =4.1.1.7 [H]

Molecular weight: Translated: 55584; Mature: 55453

Theoretical pI: Translated: 6.28; Mature: 6.28

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTVRHVTIDLLRELGMTTVFGNPGSTELPMFRDFPDDFRYVMGLQESVVLGMADGFAQG
CCCHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCHHHHHHHCCHHHEEEECCCCCCCC
TGRAAIVNLHSSAGVGHALGNLFTAFKNQTPLVVTAGQQARSILPYEPFLFAERASEFPR
CCCEEEEEECCCCCHHHHHHHHHHHHCCCCCEEEECCHHHCCCCCCCCHHHHHHHHHCCC
PFVKWSCEPARAEDVPAAIHRAWLVAMEPPCGPTFVSIPIDDWDRSCEPFAARKVHADRA
CHHEECCCCCCCCCCHHHHHHEEEEEECCCCCCEEEEEECCCCCCCCCCHHHHHHCCCCC
GDPAALAACATSMAQARRPAIVVGAGVARDGAWDRVIALAERHQAAVWVAPMSARCSFPE
CCHHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHCCCEEEEEECCCCCCCCCC
DHPLFAGFLTAGREAIVATLSSHDFVLALGGPMNLYHVEGHGPHMPEGCDVWLIGDNHIH
CCCHHHHHHHCCCCEEEEEECCCCEEEEECCCCEEEEECCCCCCCCCCCEEEEEECCEEE
AAWAPAGTAIVAQCDRALDALLAGPEPVQRDAPPVRQRLPRLDGAALTDAYVLQRLAALR
EEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHC
SPESIVVEEAPSSRGPMHEHLPILRKDTFYTTASGGLGHGLPAAVGMAMARPDDKVIALL
CCCEEEEECCCCCCCCCHHCCCCEECCCEEEECCCCCCCCCHHHHHHHHCCCCCCEEEEE
GDGSAMYAIQGLHAAAQHGLPVSFLILKNNRYEALHHFGRHFGMQQLVGTQFPELDFCKL
ECCCEEEHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHCCHHHHHCCCCCCCCHHHH
AEGHGIVARRAEDAASLDEALRWSLAADVPTLVEAVVL
CCCCCEEEECCHHHHHHHHHHHHHHHCCHHHHHHHHCC
>Mature Secondary Structure 
STVRHVTIDLLRELGMTTVFGNPGSTELPMFRDFPDDFRYVMGLQESVVLGMADGFAQG
CCHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCHHHHHHHCCHHHEEEECCCCCCCC
TGRAAIVNLHSSAGVGHALGNLFTAFKNQTPLVVTAGQQARSILPYEPFLFAERASEFPR
CCCEEEEEECCCCCHHHHHHHHHHHHCCCCCEEEECCHHHCCCCCCCCHHHHHHHHHCCC
PFVKWSCEPARAEDVPAAIHRAWLVAMEPPCGPTFVSIPIDDWDRSCEPFAARKVHADRA
CHHEECCCCCCCCCCHHHHHHEEEEEECCCCCCEEEEEECCCCCCCCCCHHHHHHCCCCC
GDPAALAACATSMAQARRPAIVVGAGVARDGAWDRVIALAERHQAAVWVAPMSARCSFPE
CCHHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHCCCEEEEEECCCCCCCCCC
DHPLFAGFLTAGREAIVATLSSHDFVLALGGPMNLYHVEGHGPHMPEGCDVWLIGDNHIH
CCCHHHHHHHCCCCEEEEEECCCCEEEEECCCCEEEEECCCCCCCCCCCEEEEEECCEEE
AAWAPAGTAIVAQCDRALDALLAGPEPVQRDAPPVRQRLPRLDGAALTDAYVLQRLAALR
EEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHC
SPESIVVEEAPSSRGPMHEHLPILRKDTFYTTASGGLGHGLPAAVGMAMARPDDKVIALL
CCCEEEEECCCCCCCCCHHCCCCEECCCEEEECCCCCCCCCHHHHHHHHCCCCCCEEEEE
GDGSAMYAIQGLHAAAQHGLPVSFLILKNNRYEALHHFGRHFGMQQLVGTQFPELDFCKL
ECCCEEEHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHCCHHHHHCCCCCCCCHHHH
AEGHGIVARRAEDAASLDEALRWSLAADVPTLVEAVVL
CCCCCEEEECCHHHHHHHHHHHHHHHCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2271624; 7663351; 9665697 [H]