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The map label for this gene is fixL [H]

Identifier: 87199594

GI number: 87199594

Start: 1632386

End: 1633933

Strand: Direct

Name: fixL [H]

Synonym: Saro_1577

Alternate gene names: 87199594

Gene position: 1632386-1633933 (Clockwise)

Preceding gene: 87199588

Following gene: 87199595

Centisome position: 45.83

GC content: 66.41

Gene sequence:

>1548_bases
TTGGATCATCTCCCTACTGTTCCGACCCTAGGGGATTTACCGGAGGTCCGGCGCGGCAACTTCACCAGCCGGTTCCTGCC
CCGGCTGTCCGTCCCGGTGGCGCTTGGCGCGGCGTTTCTCGCGATCGCGGCGGGTGCGGCGTTTCGCTTGGGGTTCGCGC
AAGCGCTCGGGCAGAGGGCGACCTTCATCTTCTTCGTGCCGGGCGTTGTCGTGGCTAGCGCCTTGTCGGGCTGGCGGGCG
GGCACGTTGGCCGCGCTCTCCGGTGCGCTTGTCGGCCTGTGGTGCGACTGGATGTCGGGCCCGCTGGAGCAGGGGAGCGT
GATCGCCGCTGTGGCATTCGTCGTCATCGGCCTCGCCGTGGCGGTGGGTGGCGAATGGTTCCAGCACGCGAGGATCGCGA
CCGAGGCGGCCGCCGCAGCGCTCGGCCGGCGCGAGACACACCTGCGCTCGATCCTTTCGACGGTTCCCGATGCCATCGTC
GTGATCGACGAGCAGGGCCTGATCCGGACCTTCAGCATTTCGGCCGAACGCATGTTCGGGTGGAGCAGCGCAGATGCCGT
TGGGCGCAACGTCAGCATCTTGATGCCTGAACCCTACCGAACGGCACACGACGGCTACTTGGACCGCTATTACCGGACCG
GAGAGAAGCGCATCATCGGCAAGGGGCGGGTTGTCGTCGGCGTGCGCAAGGACGGGTCTACCTTTCCGCTGGAGCTTGCG
GTGGGCGAGATGGAAGTCGATGGGCGGCGCCACTTCACCGGCTTCATCCGCGACCTGACGGAACGGCAGCTGGCGGAAGC
CCGCCTGCAGGAACTCCAGAACGAACTGGTCCACGTGTCCCGTCTGACGGCCTTGGGTGAGATGGCATCGGCGCTGGCGC
ACGAAATCAACCAGCCGCTTTCGGCCATAGCGAACTATCTCAGGGGTAGCGCAACGCTGCTGGCTCGCCCGGATCCACCG
CTCGACCGTGTTGCCGATGCGGTGGAGCGGGCGGCGAAAGAGGCCCTGCGCGCGGGGGACATCATTCGCCGCCTGCGCGA
CTTCGTGGGACGCGGAGAGACGGAGCGGACGGTCGAAAGCCTGCCCAAGCTGGTCGAGGAGGCCAGCGCGCTTGCCCTCG
TCGGGGCGAAGGAACACGGCATCCGCGTCCGCTACGCGTTCAGCCCTGACGTGGACCTCGTGCTGGCCGACAAGGTCCAG
ATACAGCAGGTCGTGCTCAACCTCGTCCGCAATGCGGTCGATGCGATGTCAGCGGGCGAGGGGGATGGACGGGAACTCCT
CGTCTCGATCGATCCCGACACCGACGATGCCGCGCGTGTCACCGTTTCAGACAGTGGTCCGGGGATTTCGCAAGACGTTG
CCGACCAGCTTTTCCAGCCCTTCGTGACAACCAAACGGACGGGCATGGGCGTCGGCCTTTCCATTTCACGCACGATTGTC
GAGGCGCATGGCGGCAGGATATGGGCGGAGCGCAGCGCGCTGGGCGGCGCGCAGTTCTGCTTCACCTTGCCGCGCGTCGC
CAGGGAGGAACTCTACGATGGCGAATAG

Upstream 100 bases:

>100_bases
GATGAACGGTGGGATCATTGGAACTTCTCCATGAGCGGCGCGATTTCCTTGGTGCGTCGCTGATGGAACTGATCTAAGTT
TCTGGCAGGAAGGGCGAAAT

Downstream 100 bases:

>100_bases
CGGTGCAGCCGAACCGATCGTTTACGTGGTCGACGACGACGAAAGCGCGCGACATTCGCTGGAGTTCCTGCTCGACGTGG
CGGGCATCCGTGTGCGCTCG

Product: multi-sensor signal transduction histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 515; Mature: 515

Protein sequence:

>515_residues
MDHLPTVPTLGDLPEVRRGNFTSRFLPRLSVPVALGAAFLAIAAGAAFRLGFAQALGQRATFIFFVPGVVVASALSGWRA
GTLAALSGALVGLWCDWMSGPLEQGSVIAAVAFVVIGLAVAVGGEWFQHARIATEAAAAALGRRETHLRSILSTVPDAIV
VIDEQGLIRTFSISAERMFGWSSADAVGRNVSILMPEPYRTAHDGYLDRYYRTGEKRIIGKGRVVVGVRKDGSTFPLELA
VGEMEVDGRRHFTGFIRDLTERQLAEARLQELQNELVHVSRLTALGEMASALAHEINQPLSAIANYLRGSATLLARPDPP
LDRVADAVERAAKEALRAGDIIRRLRDFVGRGETERTVESLPKLVEEASALALVGAKEHGIRVRYAFSPDVDLVLADKVQ
IQQVVLNLVRNAVDAMSAGEGDGRELLVSIDPDTDDAARVTVSDSGPGISQDVADQLFQPFVTTKRTGMGVGLSISRTIV
EAHGGRIWAERSALGGAQFCFTLPRVAREELYDGE

Sequences:

>Translated_515_residues
MDHLPTVPTLGDLPEVRRGNFTSRFLPRLSVPVALGAAFLAIAAGAAFRLGFAQALGQRATFIFFVPGVVVASALSGWRA
GTLAALSGALVGLWCDWMSGPLEQGSVIAAVAFVVIGLAVAVGGEWFQHARIATEAAAAALGRRETHLRSILSTVPDAIV
VIDEQGLIRTFSISAERMFGWSSADAVGRNVSILMPEPYRTAHDGYLDRYYRTGEKRIIGKGRVVVGVRKDGSTFPLELA
VGEMEVDGRRHFTGFIRDLTERQLAEARLQELQNELVHVSRLTALGEMASALAHEINQPLSAIANYLRGSATLLARPDPP
LDRVADAVERAAKEALRAGDIIRRLRDFVGRGETERTVESLPKLVEEASALALVGAKEHGIRVRYAFSPDVDLVLADKVQ
IQQVVLNLVRNAVDAMSAGEGDGRELLVSIDPDTDDAARVTVSDSGPGISQDVADQLFQPFVTTKRTGMGVGLSISRTIV
EAHGGRIWAERSALGGAQFCFTLPRVAREELYDGE
>Mature_515_residues
MDHLPTVPTLGDLPEVRRGNFTSRFLPRLSVPVALGAAFLAIAAGAAFRLGFAQALGQRATFIFFVPGVVVASALSGWRA
GTLAALSGALVGLWCDWMSGPLEQGSVIAAVAFVVIGLAVAVGGEWFQHARIATEAAAAALGRRETHLRSILSTVPDAIV
VIDEQGLIRTFSISAERMFGWSSADAVGRNVSILMPEPYRTAHDGYLDRYYRTGEKRIIGKGRVVVGVRKDGSTFPLELA
VGEMEVDGRRHFTGFIRDLTERQLAEARLQELQNELVHVSRLTALGEMASALAHEINQPLSAIANYLRGSATLLARPDPP
LDRVADAVERAAKEALRAGDIIRRLRDFVGRGETERTVESLPKLVEEASALALVGAKEHGIRVRYAFSPDVDLVLADKVQ
IQQVVLNLVRNAVDAMSAGEGDGRELLVSIDPDTDDAARVTVSDSGPGISQDVADQLFQPFVTTKRTGMGVGLSISRTIV
EAHGGRIWAERSALGGAQFCFTLPRVAREELYDGE

Specific function: Putative oxygen sensor; modulates the activity of fixJ, a transcriptional activator of nitrogen fixation fixK gene. FixL probably acts as a kinase that phosphorylates fixJ [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1788549, Length=381, Percent_Identity=28.6089238845144, Blast_Score=111, Evalue=1e-25,
Organism=Escherichia coli, GI1790436, Length=254, Percent_Identity=31.496062992126, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1790300, Length=375, Percent_Identity=27.2, Blast_Score=96, Evalue=6e-21,
Organism=Escherichia coli, GI48994928, Length=281, Percent_Identity=26.6903914590747, Blast_Score=72, Evalue=8e-14,
Organism=Escherichia coli, GI87081816, Length=308, Percent_Identity=30.8441558441558, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI1788393, Length=131, Percent_Identity=33.587786259542, Blast_Score=68, Evalue=2e-12,
Organism=Escherichia coli, GI1789149, Length=254, Percent_Identity=26.7716535433071, Blast_Score=66, Evalue=7e-12,
Organism=Escherichia coli, GI1790346, Length=237, Percent_Identity=26.5822784810127, Blast_Score=64, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR000700
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 55377; Mature: 55377

Theoretical pI: Translated: 6.09; Mature: 6.09

Prosite motif: PS50112 PAS ; PS50113 PAC ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDHLPTVPTLGDLPEVRRGNFTSRFLPRLSVPVALGAAFLAIAAGAAFRLGFAQALGQRA
CCCCCCCCCCCCCCHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
TFIFFVPGVVVASALSGWRAGTLAALSGALVGLWCDWMSGPLEQGSVIAAVAFVVIGLAV
EEEEEHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHH
AVGGEWFQHARIATEAAAAALGRRETHLRSILSTVPDAIVVIDEQGLIRTFSISAERMFG
HHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCEEEEECCHHHHCC
WSSADAVGRNVSILMPEPYRTAHDGYLDRYYRTGEKRIIGKGRVVVGVRKDGSTFPLELA
CCCHHHCCCCEEEECCCCCCCCCCHHHHHHHHCCCCEEEECCEEEEEEECCCCCCEEEEE
VGEMEVDGRRHFTGFIRDLTERQLAEARLQELQNELVHVSRLTALGEMASALAHEINQPL
ECCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
SAIANYLRGSATLLARPDPPLDRVADAVERAAKEALRAGDIIRRLRDFVGRGETERTVES
HHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
LPKLVEEASALALVGAKEHGIRVRYAFSPDVDLVLADKVQIQQVVLNLVRNAVDAMSAGE
HHHHHHHHHHEEEEECCCCCEEEEEEECCCCCEEEECHHHHHHHHHHHHHHHHHHHCCCC
GDGRELLVSIDPDTDDAARVTVSDSGPGISQDVADQLFQPFVTTKRTGMGVGLSISRTIV
CCCCEEEEEECCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHH
EAHGGRIWAERSALGGAQFCFTLPRVAREELYDGE
HHCCCEEEEHHHCCCCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MDHLPTVPTLGDLPEVRRGNFTSRFLPRLSVPVALGAAFLAIAAGAAFRLGFAQALGQRA
CCCCCCCCCCCCCCHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
TFIFFVPGVVVASALSGWRAGTLAALSGALVGLWCDWMSGPLEQGSVIAAVAFVVIGLAV
EEEEEHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHH
AVGGEWFQHARIATEAAAAALGRRETHLRSILSTVPDAIVVIDEQGLIRTFSISAERMFG
HHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCEEEEECCHHHHCC
WSSADAVGRNVSILMPEPYRTAHDGYLDRYYRTGEKRIIGKGRVVVGVRKDGSTFPLELA
CCCHHHCCCCEEEECCCCCCCCCCHHHHHHHHCCCCEEEECCEEEEEEECCCCCCEEEEE
VGEMEVDGRRHFTGFIRDLTERQLAEARLQELQNELVHVSRLTALGEMASALAHEINQPL
ECCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
SAIANYLRGSATLLARPDPPLDRVADAVERAAKEALRAGDIIRRLRDFVGRGETERTVES
HHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
LPKLVEEASALALVGAKEHGIRVRYAFSPDVDLVLADKVQIQQVVLNLVRNAVDAMSAGE
HHHHHHHHHHEEEEECCCCCEEEEEEECCCCCEEEECHHHHHHHHHHHHHHHHHHHCCCC
GDGRELLVSIDPDTDDAARVTVSDSGPGISQDVADQLFQPFVTTKRTGMGVGLSISRTIV
CCCCEEEEEECCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHH
EAHGGRIWAERSALGGAQFCFTLPRVAREELYDGE
HHCCCEEEEHHHCCCCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2046550; 1766374 [H]