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The map label for this gene is fixJ [H]

Identifier: 87199595

GI number: 87199595

Start: 1633923

End: 1634546

Strand: Direct

Name: fixJ [H]

Synonym: Saro_1578

Alternate gene names: 87199595

Gene position: 1633923-1634546 (Clockwise)

Preceding gene: 87199594

Following gene: 87199597

Centisome position: 45.88

GC content: 65.22

Gene sequence:

>624_bases
ATGGCGAATAGCGGTGCAGCCGAACCGATCGTTTACGTGGTCGACGACGACGAAAGCGCGCGACATTCGCTGGAGTTCCT
GCTCGACGTGGCGGGCATCCGTGTGCGCTCGTTTCCGAACGCTGACGCCTTCCTCAAATCCTCTCCGCCGCTCGCCGGGG
CCTGCATCGTTACCGACGTGCGGATGCCCGGAACAGGCGGCATCGAACTGGTCGAGGAGTTGCATCGGCGCGGCGCGACG
GCGCCAGTGATCGTCATTACCGGCCACGCGGACGTACCGCTGGCGATCCAGGCAATGAAGGCGGGTGTCGCAGACTTCAT
CGAGAAGCCGTTCGACGACCAGGTGATGCTGGAAGCGATCCGGAGCGCATTGGCTCGCAACGCCGGCGACCAGCAGGCCC
AGGCGGAGCGTCAGGCAGTGCTGGACCGCATGGCCACGCTCTCCCCGCGCGAGCGCGAGGTCGTCGTCGGGCTTGTCGCC
GGCAAGGCGAACAAGGTCATCGCCTTCGATCTCGATATCAGCGCCCGGACGGTGGAAGTCTACCGCGCCAATGCCATGAT
GAAGATGCAGGCGCGGACGCTTTCCGATCTGGTGCGCATGGTGACGCTCGCCACGCTGACCTGA

Upstream 100 bases:

>100_bases
GTCGAGGCGCATGGCGGCAGGATATGGGCGGAGCGCAGCGCGCTGGGCGGCGCGCAGTTCTGCTTCACCTTGCCGCGCGT
CGCCAGGGAGGAACTCTACG

Downstream 100 bases:

>100_bases
TGGCGCCGCGGGGTCAGCGCAGGCTGTTTGCCAGTTCGTCGCGGAAGCGGGGGTGTGCCAGGCCGATCAGCGCGTCCGCC
CGTTGCGCCACGGTCTTTCC

Product: two component LuxR family transcriptional regulator

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 207; Mature: 206

Protein sequence:

>207_residues
MANSGAAEPIVYVVDDDESARHSLEFLLDVAGIRVRSFPNADAFLKSSPPLAGACIVTDVRMPGTGGIELVEELHRRGAT
APVIVITGHADVPLAIQAMKAGVADFIEKPFDDQVMLEAIRSALARNAGDQQAQAERQAVLDRMATLSPREREVVVGLVA
GKANKVIAFDLDISARTVEVYRANAMMKMQARTLSDLVRMVTLATLT

Sequences:

>Translated_207_residues
MANSGAAEPIVYVVDDDESARHSLEFLLDVAGIRVRSFPNADAFLKSSPPLAGACIVTDVRMPGTGGIELVEELHRRGAT
APVIVITGHADVPLAIQAMKAGVADFIEKPFDDQVMLEAIRSALARNAGDQQAQAERQAVLDRMATLSPREREVVVGLVA
GKANKVIAFDLDISARTVEVYRANAMMKMQARTLSDLVRMVTLATLT
>Mature_206_residues
ANSGAAEPIVYVVDDDESARHSLEFLLDVAGIRVRSFPNADAFLKSSPPLAGACIVTDVRMPGTGGIELVEELHRRGATA
PVIVITGHADVPLAIQAMKAGVADFIEKPFDDQVMLEAIRSALARNAGDQQAQAERQAVLDRMATLSPREREVVVGLVAG
KANKVIAFDLDISARTVEVYRANAMMKMQARTLSDLVRMVTLATLT

Specific function: FixJ, when activated by fixL, induces the expression of both nifA, required for activation of classical nif and fix genes, and fixK, required for fixN activation [H]

COG id: COG4566

COG function: function code T; Response regulator

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=123, Percent_Identity=34.1463414634146, Blast_Score=81, Evalue=5e-17,
Organism=Escherichia coli, GI1788222, Length=191, Percent_Identity=30.3664921465969, Blast_Score=73, Evalue=1e-14,
Organism=Escherichia coli, GI1789809, Length=120, Percent_Identity=30.8333333333333, Blast_Score=61, Evalue=5e-11,
Organism=Escherichia coli, GI1788550, Length=102, Percent_Identity=28.4313725490196, Blast_Score=61, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001789
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 22211; Mature: 22080

Theoretical pI: Translated: 5.08; Mature: 5.08

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00622 HTH_LUXR_1 ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANSGAAEPIVYVVDDDESARHSLEFLLDVAGIRVRSFPNADAFLKSSPPLAGACIVTDV
CCCCCCCCCEEEEECCCCHHHHHHHHHHHHHCEEEECCCCCCCCCCCCCCCCCEEEEEEE
RMPGTGGIELVEELHRRGATAPVIVITGHADVPLAIQAMKAGVADFIEKPFDDQVMLEAI
CCCCCCCHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHH
RSALARNAGDQQAQAERQAVLDRMATLSPREREVVVGLVAGKANKVIAFDLDISARTVEV
HHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCEEEEEEECCCCEEEEE
YRANAMMKMQARTLSDLVRMVTLATLT
EECCHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
ANSGAAEPIVYVVDDDESARHSLEFLLDVAGIRVRSFPNADAFLKSSPPLAGACIVTDV
CCCCCCCCEEEEECCCCHHHHHHHHHHHHHCEEEECCCCCCCCCCCCCCCCCEEEEEEE
RMPGTGGIELVEELHRRGATAPVIVITGHADVPLAIQAMKAGVADFIEKPFDDQVMLEAI
CCCCCCCHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHH
RSALARNAGDQQAQAERQAVLDRMATLSPREREVVVGLVAGKANKVIAFDLDISARTVEV
HHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCEEEEEEECCCCEEEEE
YRANAMMKMQARTLSDLVRMVTLATLT
EECCHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2046550 [H]