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The map label for this gene is paaF [C]

Identifier: 87199579

GI number: 87199579

Start: 1620330

End: 1621094

Strand: Direct

Name: paaF [C]

Synonym: Saro_1562

Alternate gene names: 87199579

Gene position: 1620330-1621094 (Clockwise)

Preceding gene: 87199578

Following gene: 87199580

Centisome position: 45.49

GC content: 63.66

Gene sequence:

>765_bases
ATGAGTCAGGTAAGGTTGGACGTCAGCGATTACGTGGCCGTGGTCACGATCGACAATCCTCCGGTCAATGCCCAGAACAT
GGACCTCGTGGACGAACTGATCGCCACGTTCGACAGCTTCAACGACCGGGACGACGTGCGCGTGGCCGTACTGACCGGCG
CGGGCAAGTGCTTTTCCGCAGGTGCGGACCTGCGCAACCGACCCGACCTTTCCGCCCCCGGCGCACGCTGGGCGCGCAAC
CGCAAGGTGCGCGAAGTCAGCTATTGCATCATTGACAACGCCAAGCCGACCATCGCGGCGGTCAACGGTCCGGCACTGGG
AGCGGGGCTTGGCCTCGTGGCAAGCTGCGACATCATCGTTGCTTCCGAAAATGCCGTGTTTGGCCTGCCCGAGGTGGACG
TCGGCCTGATGGGCGGTGGAAAGCACGCCGAACGCATCCTGCCGCATTCGCTCGTGCGCCGGATGATGCTGACCGGCTAC
CGTGCGCCCGCCGAGGAGCTTTACAGGCGTGGTGTGATCGAGGCCTGCCTGCCGCCCGAGGAACTGATGCCCTGGGTTAT
GGACATGGCCCGCAACATCGCCGCCAAGAGTCCGCTGGCTACCCGCCTCGCCAAGGACAGCATGCGCACGATCGAAAACA
TGACCTTGCGTGACGGCTACGTCTACGAACAGGGCAATACCGCAAAGCTTGCCACTTCCCAAGATGCGGCAGAGGCCGTG
GCTGCTTTCGTGGAGAAGCGCCCCCCGGTTTTCCTCGGTCGCTGA

Upstream 100 bases:

>100_bases
AATCGCATATGCAATAGTTGCCCCGGGGTCCGGTGGTATCCGTGCCAAGCGGGTCATGCTTCCATAGGTTCCGGTTCAAT
CAGATTTCGGGAGAGAAGCG

Downstream 100 bases:

>100_bases
CGGGGAGCGCGCGACGATGACTGCTGGCAACTGGAACTTCGGCGACCTGCTTGATATCGCCGCCGCCAATGTCCCGACGG
ACCGTCCCGCGCTCGTGCGG

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 254; Mature: 253

Protein sequence:

>254_residues
MSQVRLDVSDYVAVVTIDNPPVNAQNMDLVDELIATFDSFNDRDDVRVAVLTGAGKCFSAGADLRNRPDLSAPGARWARN
RKVREVSYCIIDNAKPTIAAVNGPALGAGLGLVASCDIIVASENAVFGLPEVDVGLMGGGKHAERILPHSLVRRMMLTGY
RAPAEELYRRGVIEACLPPEELMPWVMDMARNIAAKSPLATRLAKDSMRTIENMTLRDGYVYEQGNTAKLATSQDAAEAV
AAFVEKRPPVFLGR

Sequences:

>Translated_254_residues
MSQVRLDVSDYVAVVTIDNPPVNAQNMDLVDELIATFDSFNDRDDVRVAVLTGAGKCFSAGADLRNRPDLSAPGARWARN
RKVREVSYCIIDNAKPTIAAVNGPALGAGLGLVASCDIIVASENAVFGLPEVDVGLMGGGKHAERILPHSLVRRMMLTGY
RAPAEELYRRGVIEACLPPEELMPWVMDMARNIAAKSPLATRLAKDSMRTIENMTLRDGYVYEQGNTAKLATSQDAAEAV
AAFVEKRPPVFLGR
>Mature_253_residues
SQVRLDVSDYVAVVTIDNPPVNAQNMDLVDELIATFDSFNDRDDVRVAVLTGAGKCFSAGADLRNRPDLSAPGARWARNR
KVREVSYCIIDNAKPTIAAVNGPALGAGLGLVASCDIIVASENAVFGLPEVDVGLMGGGKHAERILPHSLVRRMMLTGYR
APAEELYRRGVIEACLPPEELMPWVMDMARNIAAKSPLATRLAKDSMRTIENMTLRDGYVYEQGNTAKLATSQDAAEAVA
AFVEKRPPVFLGR

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=247, Percent_Identity=33.1983805668016, Blast_Score=119, Evalue=2e-27,
Organism=Homo sapiens, GI31542718, Length=246, Percent_Identity=30.0813008130081, Blast_Score=96, Evalue=2e-20,
Organism=Homo sapiens, GI70995211, Length=240, Percent_Identity=28.75, Blast_Score=89, Evalue=3e-18,
Organism=Homo sapiens, GI260274832, Length=231, Percent_Identity=28.5714285714286, Blast_Score=81, Evalue=8e-16,
Organism=Homo sapiens, GI45643119, Length=231, Percent_Identity=28.5714285714286, Blast_Score=81, Evalue=9e-16,
Organism=Homo sapiens, GI260275230, Length=231, Percent_Identity=28.5714285714286, Blast_Score=81, Evalue=9e-16,
Organism=Homo sapiens, GI68989263, Length=215, Percent_Identity=27.906976744186, Blast_Score=72, Evalue=3e-13,
Organism=Homo sapiens, GI4502327, Length=239, Percent_Identity=29.2887029288703, Blast_Score=72, Evalue=4e-13,
Organism=Homo sapiens, GI37594469, Length=209, Percent_Identity=26.3157894736842, Blast_Score=72, Evalue=7e-13,
Organism=Homo sapiens, GI37594471, Length=209, Percent_Identity=26.3157894736842, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI116235487, Length=212, Percent_Identity=25.9433962264151, Blast_Score=70, Evalue=1e-12,
Organism=Homo sapiens, GI20127408, Length=198, Percent_Identity=28.2828282828283, Blast_Score=66, Evalue=2e-11,
Organism=Homo sapiens, GI221136756, Length=238, Percent_Identity=23.5294117647059, Blast_Score=66, Evalue=4e-11,
Organism=Homo sapiens, GI221136753, Length=238, Percent_Identity=23.5294117647059, Blast_Score=66, Evalue=4e-11,
Organism=Homo sapiens, GI221307494, Length=212, Percent_Identity=24.0566037735849, Blast_Score=65, Evalue=7e-11,
Organism=Escherichia coli, GI1787659, Length=265, Percent_Identity=33.5849056603774, Blast_Score=138, Evalue=3e-34,
Organism=Escherichia coli, GI221142681, Length=248, Percent_Identity=36.6935483870968, Blast_Score=135, Evalue=2e-33,
Organism=Escherichia coli, GI87082183, Length=263, Percent_Identity=27.7566539923954, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1788597, Length=250, Percent_Identity=27.6, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1787660, Length=259, Percent_Identity=29.7297297297297, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1788682, Length=191, Percent_Identity=27.2251308900524, Blast_Score=64, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI25145438, Length=254, Percent_Identity=31.8897637795276, Blast_Score=115, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17554946, Length=246, Percent_Identity=30.8943089430894, Blast_Score=115, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17534483, Length=259, Percent_Identity=29.3436293436293, Blast_Score=94, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI17540306, Length=237, Percent_Identity=27.4261603375527, Blast_Score=92, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17536985, Length=257, Percent_Identity=29.1828793774319, Blast_Score=90, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17560910, Length=228, Percent_Identity=28.0701754385965, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI25144276, Length=183, Percent_Identity=28.9617486338798, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17508951, Length=183, Percent_Identity=28.9617486338798, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17508953, Length=183, Percent_Identity=28.9617486338798, Blast_Score=67, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI17540714, Length=201, Percent_Identity=27.8606965174129, Blast_Score=65, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17558304, Length=187, Percent_Identity=28.3422459893048, Blast_Score=65, Evalue=3e-11,
Organism=Drosophila melanogaster, GI20129971, Length=255, Percent_Identity=31.3725490196078, Blast_Score=118, Evalue=4e-27,
Organism=Drosophila melanogaster, GI24653477, Length=255, Percent_Identity=31.3725490196078, Blast_Score=118, Evalue=4e-27,
Organism=Drosophila melanogaster, GI19922422, Length=241, Percent_Identity=29.8755186721992, Blast_Score=96, Evalue=2e-20,
Organism=Drosophila melanogaster, GI19920382, Length=260, Percent_Identity=30, Blast_Score=95, Evalue=4e-20,
Organism=Drosophila melanogaster, GI24653139, Length=251, Percent_Identity=26.6932270916335, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24583165, Length=225, Percent_Identity=24.8888888888889, Blast_Score=74, Evalue=8e-14,
Organism=Drosophila melanogaster, GI19921018, Length=218, Percent_Identity=25.6880733944954, Blast_Score=66, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 27487; Mature: 27356

Theoretical pI: Translated: 5.43; Mature: 5.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQVRLDVSDYVAVVTIDNPPVNAQNMDLVDELIATFDSFNDRDDVRVAVLTGAGKCFSA
CCCEEEEHHHEEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEEECCCCHHHC
GADLRNRPDLSAPGARWARNRKVREVSYCIIDNAKPTIAAVNGPALGAGLGLVASCDIIV
CCCCCCCCCCCCCCCHHHHCCCCEEEEEEEECCCCCEEEEECCCCCHHCCHHHEECEEEE
ASENAVFGLPEVDVGLMGGGKHAERILPHSLVRRMMLTGYRAPAEELYRRGVIEACLPPE
ECCCCEECCCCCCEEEECCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCHHHHCCCHH
ELMPWVMDMARNIAAKSPLATRLAKDSMRTIENMTLRDGYVYEQGNTAKLATSQDAAEAV
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCEECCCEEEECCCCEEEECCHHHHHHH
AAFVEKRPPVFLGR
HHHHHCCCCEECCC
>Mature Secondary Structure 
SQVRLDVSDYVAVVTIDNPPVNAQNMDLVDELIATFDSFNDRDDVRVAVLTGAGKCFSA
CCEEEEHHHEEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEEECCCCHHHC
GADLRNRPDLSAPGARWARNRKVREVSYCIIDNAKPTIAAVNGPALGAGLGLVASCDIIV
CCCCCCCCCCCCCCCHHHHCCCCEEEEEEEECCCCCEEEEECCCCCHHCCHHHEECEEEE
ASENAVFGLPEVDVGLMGGGKHAERILPHSLVRRMMLTGYRAPAEELYRRGVIEACLPPE
ECCCCEECCCCCCEEEECCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCHHHHCCCHH
ELMPWVMDMARNIAAKSPLATRLAKDSMRTIENMTLRDGYVYEQGNTAKLATSQDAAEAV
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCEECCCEEEECCCCEEEECCHHHHHHH
AAFVEKRPPVFLGR
HHHHHCCCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA