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The map label for this gene is smpB

Identifier: 87199465

GI number: 87199465

Start: 1488542

End: 1489024

Strand: Direct

Name: smpB

Synonym: Saro_1444

Alternate gene names: 87199465

Gene position: 1488542-1489024 (Clockwise)

Preceding gene: 87199464

Following gene: 87199466

Centisome position: 41.79

GC content: 58.18

Gene sequence:

>483_bases
ATGGCTCGTCCCGTTCATCCCGAATTCGACAAGAAGAAGGTCGTCGCCGAAAATCGGCGGGCACGCTTCGAGTATTTCAT
CGAAGAGACCTACGAGGCTGGAATCTGCCTGACCGGCACCGAGGTGAAGTCGCTTCGCTTCGGGGAAGGTTCGATCGCCG
AGAGTTATGCCGAGGTGAAGAACGGCGAAGTCTGGTTGGTCAATTCCAACGTTCCCGAATTCAGCCACGGCAATCGTTTC
AATCACGTGCCGAAGCGTCCGCGCAAGCTATTGCTGAAAGAGCGCCAGATCGCGAAGTTTACCGGAGCCGTGGAGCGCAA
GGGCATGACGCTCGTGCCCCTGTCGATCTACTTCAACAGCCGCGGCCGTGCGAAGGTCGAACTGGCGCTCGCGAAGGGCA
AGAACGCTGCCGACAAACGCACGACGATCAAGGAACGCGATTGGAAGCGCGAAAAGGCGCGGATCATGAAGGACCACGGT
TGA

Upstream 100 bases:

>100_bases
GTCCACGCCGGACTGCTCGAACTCGCCTGAGGCACAACTGCCCGACAAAGTGTGAAGTGCCACTTCCGCTTTGCCGGGCC
AGCGCCTACATGGCGGCGAT

Downstream 100 bases:

>100_bases
CGGGCATTTTCCCTGCGGCGCTTCAGCGCGCTGAAAGAGGTGGTCGTCCAACAGTGGTCAAACGTCCCGTGGCGGGGCGG
ACGGGAAACGGGTAGAACGC

Product: SsrA-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 160; Mature: 159

Protein sequence:

>160_residues
MARPVHPEFDKKKVVAENRRARFEYFIEETYEAGICLTGTEVKSLRFGEGSIAESYAEVKNGEVWLVNSNVPEFSHGNRF
NHVPKRPRKLLLKERQIAKFTGAVERKGMTLVPLSIYFNSRGRAKVELALAKGKNAADKRTTIKERDWKREKARIMKDHG

Sequences:

>Translated_160_residues
MARPVHPEFDKKKVVAENRRARFEYFIEETYEAGICLTGTEVKSLRFGEGSIAESYAEVKNGEVWLVNSNVPEFSHGNRF
NHVPKRPRKLLLKERQIAKFTGAVERKGMTLVPLSIYFNSRGRAKVELALAKGKNAADKRTTIKERDWKREKARIMKDHG
>Mature_159_residues
ARPVHPEFDKKKVVAENRRARFEYFIEETYEAGICLTGTEVKSLRFGEGSIAESYAEVKNGEVWLVNSNVPEFSHGNRFN
HVPKRPRKLLLKERQIAKFTGAVERKGMTLVPLSIYFNSRGRAKVELALAKGKNAADKRTTIKERDWKREKARIMKDHG

Specific function: Binds specifically to the ssrA RNA (tmRNA) and is required for stable association of ssrA with ribosomes

COG id: COG0691

COG function: function code O; tmRNA-binding protein

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the smpB family

Homologues:

Organism=Escherichia coli, GI1788973, Length=148, Percent_Identity=46.6216216216216, Blast_Score=130, Evalue=6e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SSRP_NOVAD (Q2G8D5)

Other databases:

- EMBL:   CP000248
- RefSeq:   YP_496722.1
- ProteinModelPortal:   Q2G8D5
- SMR:   Q2G8D5
- STRING:   Q2G8D5
- GeneID:   3916108
- GenomeReviews:   CP000248_GR
- KEGG:   nar:Saro_1444
- NMPDR:   fig|48935.1.peg.2226
- eggNOG:   COG0691
- HOGENOM:   HBG293422
- OMA:   EKYEAGI
- PhylomeDB:   Q2G8D5
- ProtClustDB:   PRK05422
- BioCyc:   NARO279238:SARO_1444-MONOMER
- GO:   GO:0005737
- GO:   GO:0006412
- HAMAP:   MF_00023
- InterPro:   IPR000037
- InterPro:   IPR020081
- Gene3D:   G3DSA:2.40.280.10
- ProDom:   PD004488
- TIGRFAMs:   TIGR00086

Pfam domain/function: PF01668 SmpB; SSF74982 SmpB

EC number: NA

Molecular weight: Translated: 18404; Mature: 18273

Theoretical pI: Translated: 10.67; Mature: 10.67

Prosite motif: PS01317 SSRP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARPVHPEFDKKKVVAENRRARFEYFIEETYEAGICLTGTEVKSLRFGEGSIAESYAEVK
CCCCCCCCCCCHHHHHHCHHHHHHHHHHHHCCCCEEEECCCCCEEECCCCHHHHHHHHHC
NGEVWLVNSNVPEFSHGNRFNHVPKRPRKLLLKERQIAKFTGAVERKGMTLVPLSIYFNS
CCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEECC
RGRAKVELALAKGKNAADKRTTIKERDWKREKARIMKDHG
CCCEEEEEEEECCCCCCCHHCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
ARPVHPEFDKKKVVAENRRARFEYFIEETYEAGICLTGTEVKSLRFGEGSIAESYAEVK
CCCCCCCCCCHHHHHHCHHHHHHHHHHHHCCCCEEEECCCCCEEECCCCHHHHHHHHHC
NGEVWLVNSNVPEFSHGNRFNHVPKRPRKLLLKERQIAKFTGAVERKGMTLVPLSIYFNS
CCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEECC
RGRAKVELALAKGKNAADKRTTIKERDWKREKARIMKDHG
CCCEEEEEEEECCCCCCCHHCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA