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The map label for this gene is dapA

Identifier: 87199464

GI number: 87199464

Start: 1487581

End: 1488471

Strand: Direct

Name: dapA

Synonym: Saro_1443

Alternate gene names: 87199464

Gene position: 1487581-1488471 (Clockwise)

Preceding gene: 87199458

Following gene: 87199465

Centisome position: 41.77

GC content: 63.97

Gene sequence:

>891_bases
ATGTTTTCCGGCTCGATTCCGGCCCTTGTGACTCCTTTTCGCGATGGAGCGTTCGATGAGAAGGCGTTCCGCCGCCTTGT
CGACTGGCAGATCGAGAACGGCTCTTCGGCGCTCGTTCCCTGCGGCACCACCGGCGAGGCTTCGACGCTTTCGAACGCCG
AGCATCACCGCGTCATCGAAGTCTGCGTGGAACAGGCGGCCGGCCGCGTTCCCGTGATCGCCGGCTGCGGCAGCAATGAC
ACGATGAATGCCTTGCTGCACATGAACTTTTCCAAGAAGTGCGGTGCGCAGGCCGCGCTCTGCGTTGCGCCCTACTATAA
CCGGCCGAGCCAGGCTGGCATCATTGCGCACTTCAGCTACCTGGCGGAGCACAACGACCTGCCGATCGTGCTCTACAACG
TACCTGGCCGCACCGTGACCGACATCCTGCCGGAGACTGTGTGCGAACTCGCCAAGCGCTATCCGGACAAGATCATCGGC
ATCAAGGATGCCAGCGGCGATCTGTCGCGCGTGACCGACCACCGCATGGGGATCGGCAAGCATTTCTGCCAGCTTTCCGG
CGACGACGAGCTGGCGCTGCCCGCCAATGCCGCCGGGGCGGTCGGTTGCATCTCCGTCACGGCTAACGTGGCGCCCAGGC
TCTGCGCCGATTTCCAGGCTGCCTGCGCCGCGAACGATCTGGAGAAGGCGCGAGAACTGAACGACAAGCTCTATCCGCTG
CATTACGCCATGTTCGAGGACGCTTCGCCGGGGCCGGTGAAGTACGCGCTGAGCCGGGTGTTCGCGGACATCAACGAAGA
CCTGCGCCTTCCGATGGTCCCGTGCAACGAGGCTGCCCGCAAGGCTGTCGACGCTGCGCTGGTCCACGCCGGACTGCTCG
AACTCGCCTGA

Upstream 100 bases:

>100_bases
ATACTATGGAGCGGCTCCTTATCAGGCGCTGAACAGACCCCCGTGGGCAAAGTGCGCGATGCACCGAAGCAACCATGCGG
CAAATTGAGGGATATTGTCC

Downstream 100 bases:

>100_bases
GGCACAACTGCCCGACAAAGTGTGAAGTGCCACTTCCGCTTTGCCGGGCCAGCGCCTACATGGCGGCGATATGGCTCGTC
CCGTTCATCCCGAATTCGAC

Product: dihydrodipicolinate synthase

Products: NA

Alternate protein names: DHDPS

Number of amino acids: Translated: 296; Mature: 296

Protein sequence:

>296_residues
MFSGSIPALVTPFRDGAFDEKAFRRLVDWQIENGSSALVPCGTTGEASTLSNAEHHRVIEVCVEQAAGRVPVIAGCGSND
TMNALLHMNFSKKCGAQAALCVAPYYNRPSQAGIIAHFSYLAEHNDLPIVLYNVPGRTVTDILPETVCELAKRYPDKIIG
IKDASGDLSRVTDHRMGIGKHFCQLSGDDELALPANAAGAVGCISVTANVAPRLCADFQAACAANDLEKARELNDKLYPL
HYAMFEDASPGPVKYALSRVFADINEDLRLPMVPCNEAARKAVDAALVHAGLLELA

Sequences:

>Translated_296_residues
MFSGSIPALVTPFRDGAFDEKAFRRLVDWQIENGSSALVPCGTTGEASTLSNAEHHRVIEVCVEQAAGRVPVIAGCGSND
TMNALLHMNFSKKCGAQAALCVAPYYNRPSQAGIIAHFSYLAEHNDLPIVLYNVPGRTVTDILPETVCELAKRYPDKIIG
IKDASGDLSRVTDHRMGIGKHFCQLSGDDELALPANAAGAVGCISVTANVAPRLCADFQAACAANDLEKARELNDKLYPL
HYAMFEDASPGPVKYALSRVFADINEDLRLPMVPCNEAARKAVDAALVHAGLLELA
>Mature_296_residues
MFSGSIPALVTPFRDGAFDEKAFRRLVDWQIENGSSALVPCGTTGEASTLSNAEHHRVIEVCVEQAAGRVPVIAGCGSND
TMNALLHMNFSKKCGAQAALCVAPYYNRPSQAGIIAHFSYLAEHNDLPIVLYNVPGRTVTDILPETVCELAKRYPDKIIG
IKDASGDLSRVTDHRMGIGKHFCQLSGDDELALPANAAGAVGCISVTANVAPRLCADFQAACAANDLEKARELNDKLYPL
HYAMFEDASPGPVKYALSRVFADINEDLRLPMVPCNEAARKAVDAALVHAGLLELA

Specific function: Biosynthesis of diaminopimelate and lysine from aspartate semialdehyde; first step. [C]

COG id: COG0329

COG function: function code EM; Dihydrodipicolinate synthase/N-acetylneuraminate lyase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DHDPS family

Homologues:

Organism=Homo sapiens, GI31543060, Length=244, Percent_Identity=29.0983606557377, Blast_Score=83, Evalue=4e-16,
Organism=Escherichia coli, GI1788823, Length=294, Percent_Identity=45.578231292517, Blast_Score=243, Evalue=1e-65,
Organism=Escherichia coli, GI87082415, Length=298, Percent_Identity=32.2147651006711, Blast_Score=133, Evalue=1e-32,
Organism=Escherichia coli, GI1786463, Length=234, Percent_Identity=31.1965811965812, Blast_Score=107, Evalue=9e-25,
Organism=Escherichia coli, GI1789620, Length=237, Percent_Identity=29.1139240506329, Blast_Score=88, Evalue=8e-19,

Paralogues:

None

Copy number: 840 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): DAPA_NOVAD (Q2G8D6)

Other databases:

- EMBL:   CP000248
- RefSeq:   YP_496721.1
- HSSP:   Q9X1K9
- ProteinModelPortal:   Q2G8D6
- SMR:   Q2G8D6
- STRING:   Q2G8D6
- GeneID:   3916107
- GenomeReviews:   CP000248_GR
- KEGG:   nar:Saro_1443
- NMPDR:   fig|48935.1.peg.2227
- eggNOG:   COG0329
- HOGENOM:   HBG358848
- OMA:   YYNRPSD
- PhylomeDB:   Q2G8D6
- ProtClustDB:   PRK03170
- BioCyc:   NARO279238:SARO_1443-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00418
- InterPro:   IPR013785
- InterPro:   IPR005263
- InterPro:   IPR002220
- InterPro:   IPR020624
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR12128
- PRINTS:   PR00146
- TIGRFAMs:   TIGR00674

Pfam domain/function: PF00701 DHDPS

EC number: =4.2.1.52

Molecular weight: Translated: 31695; Mature: 31695

Theoretical pI: Translated: 5.56; Mature: 5.56

Prosite motif: PS00665 DHDPS_1; PS00666 DHDPS_2

Important sites: ACT_SITE 162-162 BINDING 105-105

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
3.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFSGSIPALVTPFRDGAFDEKAFRRLVDWQIENGSSALVPCGTTGEASTLSNAEHHRVIE
CCCCCCCCEECCCCCCCCCHHHHHHHHCEEECCCCCEEEECCCCCCCHHCCCCHHHHHHH
VCVEQAAGRVPVIAGCGSNDTMNALLHMNFSKKCGAQAALCVAPYYNRPSQAGIIAHFSY
HHHHHHCCCCCEEEECCCCCCEEEEEECCCCHHCCCCEEEEEECCCCCCCCCHHHHHHHH
LAEHNDLPIVLYNVPGRTVTDILPETVCELAKRYPDKIIGIKDASGDLSRVTDHRMGIGK
HHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCHHEEEEECCCCCHHHHHHHHHHHHH
HFCQLSGDDELALPANAAGAVGCISVTANVAPRLCADFQAACAANDLEKARELNDKLYPL
HHHHCCCCCCEECCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEE
HYAMFEDASPGPVKYALSRVFADINEDLRLPMVPCNEAARKAVDAALVHAGLLELA
EHEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MFSGSIPALVTPFRDGAFDEKAFRRLVDWQIENGSSALVPCGTTGEASTLSNAEHHRVIE
CCCCCCCCEECCCCCCCCCHHHHHHHHCEEECCCCCEEEECCCCCCCHHCCCCHHHHHHH
VCVEQAAGRVPVIAGCGSNDTMNALLHMNFSKKCGAQAALCVAPYYNRPSQAGIIAHFSY
HHHHHHCCCCCEEEECCCCCCEEEEEECCCCHHCCCCEEEEEECCCCCCCCCHHHHHHHH
LAEHNDLPIVLYNVPGRTVTDILPETVCELAKRYPDKIIGIKDASGDLSRVTDHRMGIGK
HHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCHHEEEEECCCCCHHHHHHHHHHHHH
HFCQLSGDDELALPANAAGAVGCISVTANVAPRLCADFQAACAANDLEKARELNDKLYPL
HHHHCCCCCCEECCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEE
HYAMFEDASPGPVKYALSRVFADINEDLRLPMVPCNEAARKAVDAALVHAGLLELA
EHEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA