Click here to switch to the map view.

The map label for this gene is rlmN

Identifier: 87199457

GI number: 87199457

Start: 1481281

End: 1482570

Strand: Reverse

Name: rlmN

Synonym: Saro_1436

Alternate gene names: 87199457

Gene position: 1482570-1481281 (Counterclockwise)

Preceding gene: 87199459

Following gene: 87199455

Centisome position: 41.63

GC content: 62.87

Gene sequence:

>1290_bases
ATGCGCGCGATGCAGACCCACACCGAAATTGCTCCCATGCCCATCCCCGGGCACGTCGATCCCGTTCCCGTTCCGCGCGA
AGTCACTCCGCGTGAGGACGGACGCATCGACCTGATCGGGCTACCAAGGAAGCAGATCGCCGAACTGTTTGCGCAAGCCG
GACTGGACGCGAAGGCCGCGAAACTGCGGGCCAAGCAGGTATTTCACTGGCTCTATCACCGCGGCGTCACCGATTTCGAC
GCCATGACCGACATCGCCAAGACCATGCGCCCCTGGCTGGCCGAACGCTTCGTCATCGGCCGTCCCGAAATCGTCGAAGC
GCAGGTCTCGACCGACGGCACCCGGAAGTGGCTGCTTCGCACGGCCGACAAGCATGACTTCGAAATGGTGTTCATTCCCG
ACGCCGACCGCGGCACGCTTTGCGTTTCGAGCCAGGTCGGTTGCACGCTGAACTGCCGGTTCTGCCATACCGGAACCATG
CGTCTGGTACGCAACCTGACCCCGGGCGAGATCGTCGGCCAGGTCATGCTGGCGCGTGATGCGCTGGGCGAATGGCCCAA
GGGCGCCAACGATTCCCGCGTTGCCACCATGGCTGGCCTGGATTTCGATGACGAGGACGAAGGTAGCTATACTTCGGACG
GGCGCCTTCTCACCAACATCGTGATGATGGGCATGGGCGAGCCGCTCTACAACTTCGACAATGTCCGCGATGCGCTCAAG
CTCGTCATGGACGGGGATGGCCTTGCCCTGTCGAAGCGCCGCATCACGCTGTCCACCAGTGGCGTGGTTCCGATGATGGA
GCGCTGCGGCGAGGAAATCGGCGTGAACCTTGCGGTTTCGCTCCACGCGGTCACCAAGGACGTGCGCGACGAGATCGTAC
CGATCAACCGCAAGTACGGGATCGAGGAACTGCTCCAGGCGTGCGCTGACTATCCGGGTGCTTCCAACGCCCGGCGCATC
ACCTTCGAATACGTGATGCTTAAGGACAAGAACGACAGCGACGACCATGCGCGCGAGCTCGTTCGCCTGATCCGTCAGTA
CAAGCTTCCCGCAAAGGTCAACCTGATCCCGTTCAACCCGTGGCCGGGAGCGCCTTACGAGTGCTCGAGCCCGGATCGGA
TAAAGAGCTTCGCCAACATCGTGTTCGAAGCCGGCATCAGCGCACCTGTGCGAACTCCGCGCGGGCGCGACATCGATGCG
GCCTGCGGGCAGTTGAAGACGGCTTCTGAACGCAAGAGCCGCGCCGAGCTTGACCGCTTGGCGGAGGAAAAGCTCGCGGC
GCTGGGCTGA

Upstream 100 bases:

>100_bases
CCGTTGTGGTTGGACTGTTGCCAGCGTGCGATGGGGTGCGAATGATCGCCTGTCGGCCGGATTTCGCGCACCGTTTGCGC
CCGCGCACGCGACGCACTAT

Downstream 100 bases:

>100_bases
GCCTTCGGCTTAGCCAACCTTGCGCGACGCAACGACCTCTTGTTCTGCAATCGCGAACCGCACCCCGACGGGGGCGGCAA
CGGCTGCGATCCGCCCGTTC

Product: hypothetical protein

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase

Number of amino acids: Translated: 429; Mature: 429

Protein sequence:

>429_residues
MRAMQTHTEIAPMPIPGHVDPVPVPREVTPREDGRIDLIGLPRKQIAELFAQAGLDAKAAKLRAKQVFHWLYHRGVTDFD
AMTDIAKTMRPWLAERFVIGRPEIVEAQVSTDGTRKWLLRTADKHDFEMVFIPDADRGTLCVSSQVGCTLNCRFCHTGTM
RLVRNLTPGEIVGQVMLARDALGEWPKGANDSRVATMAGLDFDDEDEGSYTSDGRLLTNIVMMGMGEPLYNFDNVRDALK
LVMDGDGLALSKRRITLSTSGVVPMMERCGEEIGVNLAVSLHAVTKDVRDEIVPINRKYGIEELLQACADYPGASNARRI
TFEYVMLKDKNDSDDHARELVRLIRQYKLPAKVNLIPFNPWPGAPYECSSPDRIKSFANIVFEAGISAPVRTPRGRDIDA
ACGQLKTASERKSRAELDRLAEEKLAALG

Sequences:

>Translated_429_residues
MRAMQTHTEIAPMPIPGHVDPVPVPREVTPREDGRIDLIGLPRKQIAELFAQAGLDAKAAKLRAKQVFHWLYHRGVTDFD
AMTDIAKTMRPWLAERFVIGRPEIVEAQVSTDGTRKWLLRTADKHDFEMVFIPDADRGTLCVSSQVGCTLNCRFCHTGTM
RLVRNLTPGEIVGQVMLARDALGEWPKGANDSRVATMAGLDFDDEDEGSYTSDGRLLTNIVMMGMGEPLYNFDNVRDALK
LVMDGDGLALSKRRITLSTSGVVPMMERCGEEIGVNLAVSLHAVTKDVRDEIVPINRKYGIEELLQACADYPGASNARRI
TFEYVMLKDKNDSDDHARELVRLIRQYKLPAKVNLIPFNPWPGAPYECSSPDRIKSFANIVFEAGISAPVRTPRGRDIDA
ACGQLKTASERKSRAELDRLAEEKLAALG
>Mature_429_residues
MRAMQTHTEIAPMPIPGHVDPVPVPREVTPREDGRIDLIGLPRKQIAELFAQAGLDAKAAKLRAKQVFHWLYHRGVTDFD
AMTDIAKTMRPWLAERFVIGRPEIVEAQVSTDGTRKWLLRTADKHDFEMVFIPDADRGTLCVSSQVGCTLNCRFCHTGTM
RLVRNLTPGEIVGQVMLARDALGEWPKGANDSRVATMAGLDFDDEDEGSYTSDGRLLTNIVMMGMGEPLYNFDNVRDALK
LVMDGDGLALSKRRITLSTSGVVPMMERCGEEIGVNLAVSLHAVTKDVRDEIVPINRKYGIEELLQACADYPGASNARRI
TFEYVMLKDKNDSDDHARELVRLIRQYKLPAKVNLIPFNPWPGAPYECSSPDRIKSFANIVFEAGISAPVRTPRGRDIDA
ACGQLKTASERKSRAELDRLAEEKLAALG

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family

Homologues:

Organism=Escherichia coli, GI1788865, Length=383, Percent_Identity=46.9973890339426, Blast_Score=325, Evalue=4e-90,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMN_NOVAD (Q2G8E3)

Other databases:

- EMBL:   CP000248
- RefSeq:   YP_496714.1
- ProteinModelPortal:   Q2G8E3
- STRING:   Q2G8E3
- GeneID:   3916100
- GenomeReviews:   CP000248_GR
- KEGG:   nar:Saro_1436
- NMPDR:   fig|48935.1.peg.2232
- eggNOG:   COG0820
- HOGENOM:   HBG335345
- OMA:   TFCSTAQ
- PhylomeDB:   Q2G8E3
- ProtClustDB:   CLSK835312
- BioCyc:   NARO279238:SARO_1436-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01849
- InterPro:   IPR004383
- PIRSF:   PIRSF006004
- TIGRFAMs:   TIGR00048

Pfam domain/function: NA

EC number: =2.1.1.192

Molecular weight: Translated: 47675; Mature: 47675

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRAMQTHTEIAPMPIPGHVDPVPVPREVTPREDGRIDLIGLPRKQIAELFAQAGLDAKAA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHCCCCHHHH
KLRAKQVFHWLYHRGVTDFDAMTDIAKTMRPWLAERFVIGRPEIVEAQVSTDGTRKWLLR
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHEECCCCCEEEEECCCCCHHHHHHC
TADKHDFEMVFIPDADRGTLCVSSQVGCTLNCRFCHTGTMRLVRNLTPGEIVGQVMLARD
CCCCCCEEEEEECCCCCCCEEEECCCCEEEEEEEECCCHHHHHHCCCHHHHHHHHHHHHH
ALGEWPKGANDSRVATMAGLDFDDEDEGSYTSDGRLLTNIVMMGMGEPLYNFDNVRDALK
HHCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHH
LVMDGDGLALSKRRITLSTSGVVPMMERCGEEIGVNLAVSLHAVTKDVRDEIVPINRKYG
HHCCCCCCEEECEEEEEECCCCHHHHHHCCHHHCCEEEEEHHHHHHHHHHHHCCCCCCCC
IEELLQACADYPGASNARRITFEYVMLKDKNDSDDHARELVRLIRQYKLPAKVNLIPFNP
HHHHHHHHHCCCCCCCCEEEEEEEEEEECCCCCHHHHHHHHHHHHHHCCCCEEEEEECCC
WPGAPYECSSPDRIKSFANIVFEAGISAPVRTPRGRDIDAACGQLKTASERKSRAELDRL
CCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
AEEKLAALG
HHHHHHHCC
>Mature Secondary Structure
MRAMQTHTEIAPMPIPGHVDPVPVPREVTPREDGRIDLIGLPRKQIAELFAQAGLDAKAA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHCCCCHHHH
KLRAKQVFHWLYHRGVTDFDAMTDIAKTMRPWLAERFVIGRPEIVEAQVSTDGTRKWLLR
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHEECCCCCEEEEECCCCCHHHHHHC
TADKHDFEMVFIPDADRGTLCVSSQVGCTLNCRFCHTGTMRLVRNLTPGEIVGQVMLARD
CCCCCCEEEEEECCCCCCCEEEECCCCEEEEEEEECCCHHHHHHCCCHHHHHHHHHHHHH
ALGEWPKGANDSRVATMAGLDFDDEDEGSYTSDGRLLTNIVMMGMGEPLYNFDNVRDALK
HHCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHH
LVMDGDGLALSKRRITLSTSGVVPMMERCGEEIGVNLAVSLHAVTKDVRDEIVPINRKYG
HHCCCCCCEEECEEEEEECCCCHHHHHHCCHHHCCEEEEEHHHHHHHHHHHHCCCCCCCC
IEELLQACADYPGASNARRITFEYVMLKDKNDSDDHARELVRLIRQYKLPAKVNLIPFNP
HHHHHHHHHCCCCCCCCEEEEEEEEEEECCCCCHHHHHHHHHHHHHHCCCCEEEEEECCC
WPGAPYECSSPDRIKSFANIVFEAGISAPVRTPRGRDIDAACGQLKTASERKSRAELDRL
CCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
AEEKLAALG
HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA