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The map label for this gene is phaB [H]

Identifier: 87199455

GI number: 87199455

Start: 1477676

End: 1478461

Strand: Reverse

Name: phaB [H]

Synonym: Saro_1434

Alternate gene names: 87199455

Gene position: 1478461-1477676 (Counterclockwise)

Preceding gene: 87199457

Following gene: 87199454

Centisome position: 41.51

GC content: 63.74

Gene sequence:

>786_bases
ATGATCGGCGTCGCAGGACGCAGGCTACATGTCGCACGATGGCATTCCGGCGCGAGCGAACGCCCGCCGCTGCTGCTGCT
GAACGGCATCGGCATGAATCTGGAGCTGCTTGCGCCAGTGGCGCGCAGCTTGCCCGAACGGGAAGTGATCTGCTTCGACA
TGCCGGGGACAGGCAACTCGCCGGATCCGGTATTTCCCTACTCTATCAGTTGCATGGCCCTGACCACGGCCAGCCTTCTC
GACCGGCTCGAAATCGATCGCGCAGATGTTCTCGGCATCAGCTGGGGCGGCGCCGTGGCCCAGCAGTTCGCGTTCCAGCA
TCGGGCTCGCATCGGCAAGCTGGTGCTTGCCGCAACCGCTGCTGGCGCGACGATGATCCCCGGCAACGCCGCCCTGCTCG
CCCATCTCTCCGACCCGCGCGAGTATTCAGTGGAGCGCACGCTCAAGCGCAACCTCGCCTCGATCTATAATGGCGGCGGA
AGCGCTCGGGTCTCGCTCAACGCGGCAAAGGCCCCCTCGCCGCTAGGCTTCTCCTATCAGATGGCAGCTTTCGCCAGTTG
GTCGAGCTTGCCGATCCTGCCGATGCTCTCGATGCCGGTCATGGTGATGGCCGACGAGGAAGACCAGCTCGTTCCTCCTT
CCAACGCGCATTTCCTGCATCACGCGATTGCGCACAGCGAACTGGAGATGTTCCGCGGCGGCGGTCATCTCTTCATGCTG
TCCCAGCGCGAAAGGTTCGTGGCCAGACTGAGGACGTTCCTCGACGACGACGCACACGCGTGTTGA

Upstream 100 bases:

>100_bases
CCGTTTTGAGAAACAGTTAAGCTTCATACCAATCAGGCGCTATTGCTGCGACTGCGAAGGTGCTAGTGTCGCCCGCGTGA
CCGATACCGCGATACACAAG

Downstream 100 bases:

>100_bases
TATCGGGCATCGCCGCTTTCCTTTTGGCGATCATGCGATAGCATCCGGCGAAAGACTCGTCGGGAGAGACTATGCGGCAT
ATCGCGATCATCGGCTCGGG

Product: alpha/beta hydrolase

Products: NA

Alternate protein names: PHA depolymerase; PHB depolymerase [H]

Number of amino acids: Translated: 261; Mature: 261

Protein sequence:

>261_residues
MIGVAGRRLHVARWHSGASERPPLLLLNGIGMNLELLAPVARSLPEREVICFDMPGTGNSPDPVFPYSISCMALTTASLL
DRLEIDRADVLGISWGGAVAQQFAFQHRARIGKLVLAATAAGATMIPGNAALLAHLSDPREYSVERTLKRNLASIYNGGG
SARVSLNAAKAPSPLGFSYQMAAFASWSSLPILPMLSMPVMVMADEEDQLVPPSNAHFLHHAIAHSELEMFRGGGHLFML
SQRERFVARLRTFLDDDAHAC

Sequences:

>Translated_261_residues
MIGVAGRRLHVARWHSGASERPPLLLLNGIGMNLELLAPVARSLPEREVICFDMPGTGNSPDPVFPYSISCMALTTASLL
DRLEIDRADVLGISWGGAVAQQFAFQHRARIGKLVLAATAAGATMIPGNAALLAHLSDPREYSVERTLKRNLASIYNGGG
SARVSLNAAKAPSPLGFSYQMAAFASWSSLPILPMLSMPVMVMADEEDQLVPPSNAHFLHHAIAHSELEMFRGGGHLFML
SQRERFVARLRTFLDDDAHAC
>Mature_261_residues
MIGVAGRRLHVARWHSGASERPPLLLLNGIGMNLELLAPVARSLPEREVICFDMPGTGNSPDPVFPYSISCMALTTASLL
DRLEIDRADVLGISWGGAVAQQFAFQHRARIGKLVLAATAAGATMIPGNAALLAHLSDPREYSVERTLKRNLASIYNGGG
SARVSLNAAKAPSPLGFSYQMAAFASWSSLPILPMLSMPVMVMADEEDQLVPPSNAHFLHHAIAHSELEMFRGGGHLFML
SQRERFVARLRTFLDDDAHAC

Specific function: PHA depolymerase is in fact a lipase [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. Lipase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR011942 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: NA

Molecular weight: Translated: 28223; Mature: 28223

Theoretical pI: Translated: 7.35; Mature: 7.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIGVAGRRLHVARWHSGASERPPLLLLNGIGMNLELLAPVARSLPEREVICFDMPGTGNS
CCCCCCCEEEEEEECCCCCCCCCEEEEECCCCCHHHHHHHHHCCCCCCEEEEECCCCCCC
PDPVFPYSISCMALTTASLLDRLEIDRADVLGISWGGAVAQQFAFQHRARIGKLVLAATA
CCCCCCCEEHHHHHHHHHHHHHHCCCCHHEEEEECCHHHHHHHHHHHHHHHHHEEEEEEC
AGATMIPGNAALLAHLSDPREYSVERTLKRNLASIYNGGGSARVSLNAAKAPSPLGFSYQ
CCCEECCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCH
MAAFASWSSLPILPMLSMPVMVMADEEDQLVPPSNAHFLHHAIAHSELEMFRGGGHLFML
HHHHHCCCCCCCCHHHCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEE
SQRERFVARLRTFLDDDAHAC
CHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MIGVAGRRLHVARWHSGASERPPLLLLNGIGMNLELLAPVARSLPEREVICFDMPGTGNS
CCCCCCCEEEEEEECCCCCCCCCEEEEECCCCCHHHHHHHHHCCCCCCEEEEECCCCCCC
PDPVFPYSISCMALTTASLLDRLEIDRADVLGISWGGAVAQQFAFQHRARIGKLVLAATA
CCCCCCCEEHHHHHHHHHHHHHHCCCCHHEEEEECCHHHHHHHHHHHHHHHHHEEEEEEC
AGATMIPGNAALLAHLSDPREYSVERTLKRNLASIYNGGGSARVSLNAAKAPSPLGFSYQ
CCCEECCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCH
MAAFASWSSLPILPMLSMPVMVMADEEDQLVPPSNAHFLHHAIAHSELEMFRGGGHLFML
HHHHHCCCCCCCCHHHCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEE
SQRERFVARLRTFLDDDAHAC
CHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1989978 [H]