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The map label for this gene is dapF

Identifier: 87199423

GI number: 87199423

Start: 1444680

End: 1445507

Strand: Reverse

Name: dapF

Synonym: Saro_1402

Alternate gene names: 87199423

Gene position: 1445507-1444680 (Counterclockwise)

Preceding gene: 87199424

Following gene: 87199422

Centisome position: 40.59

GC content: 66.55

Gene sequence:

>828_bases
ATGCCTAAGTCGCGCGGCATGCGCATTCCCTTCCGCAAGATGCACGGGCTCGGCAACGATTTCGTGGTGATCGACGCCCG
CGAGGCGGCCGTTTCCATGACCGCCGCGCGTGCGGCGGCGCTCGCCGACCGCCGCACCGGCATCGGTTGCGACCAGCTGA
TCGTTCTTGAACCGTCGGACGTGGCCGACGTGCGGATGCGCATCTTCAACAACGACGGCAGCGAGGTCGAGGCCTGCGGC
AACGCCAGCCGCGCCGTCGGCCTCCTCCTTGGCGGCACGCAATTCATTGAAACGCTTGGCGGACTGATCCGTTCGGAAGC
ACGGCCCGATGGCGTCTCTGTAGACATGGGCGCGCCCCGTCTGCGCTGGGATGAAATCCCGGTCTCCTATGCGATGGACA
CGCTGGAAATGCCGGTCGGCTGGGAGGACCTGGAGAACCCGGTCGGAGTCAATGTCGGCAACCCGCACGTGATCTTCTTC
GTGGACGATGCGGACGCCGTCGATCTCGCCCGTCTCGGCCCGCTGATCGAGAACGATCCGCTTTTTCCCGAGAGAATCAA
CGTCAATGTCGCCAGCGTGGTTGGCCCCGACCATCTCAAGCTGCGCGTGTGGGAACGCGGTGCCGGTCTTACCCGGGCCT
GCGGAACCGGCGCCTGCGCGACGGCGGTCGCCGCGATCCGCCGCAAGCTGACGGGCCGCAAGGTCCGGATCGACCTGCCC
GGCGGCCCGCTCACCATCACCTGGCCTGAAGGCGGGACCATCGATATGACCGGGCCTGCGACGCTGAGCTTCGAAGGCGC
GTTCGAGGACTCAGACTTCCCGGCATGA

Upstream 100 bases:

>100_bases
CGGAAAATCAGTCCGGCATCCCGGCCCGTACCGCAATCGCCTGAGAGCTGCTGTGAAAGCTGCGGTTTGAGCCGCCGACC
GATTGCCATGCCGCCTGCGA

Downstream 100 bases:

>100_bases
GCGTCGAGGTCATATCCCTCGGCTGCCGCCTCAACATCGCCGAAAGCGAGGCGATACGGGGACTTATCGCGGACGGGCCG
CCCACCGTCGTCGTCAATTC

Product: diaminopimelate epimerase

Products: NA

Alternate protein names: DAP epimerase [H]

Number of amino acids: Translated: 275; Mature: 274

Protein sequence:

>275_residues
MPKSRGMRIPFRKMHGLGNDFVVIDAREAAVSMTAARAAALADRRTGIGCDQLIVLEPSDVADVRMRIFNNDGSEVEACG
NASRAVGLLLGGTQFIETLGGLIRSEARPDGVSVDMGAPRLRWDEIPVSYAMDTLEMPVGWEDLENPVGVNVGNPHVIFF
VDDADAVDLARLGPLIENDPLFPERINVNVASVVGPDHLKLRVWERGAGLTRACGTGACATAVAAIRRKLTGRKVRIDLP
GGPLTITWPEGGTIDMTGPATLSFEGAFEDSDFPA

Sequences:

>Translated_275_residues
MPKSRGMRIPFRKMHGLGNDFVVIDAREAAVSMTAARAAALADRRTGIGCDQLIVLEPSDVADVRMRIFNNDGSEVEACG
NASRAVGLLLGGTQFIETLGGLIRSEARPDGVSVDMGAPRLRWDEIPVSYAMDTLEMPVGWEDLENPVGVNVGNPHVIFF
VDDADAVDLARLGPLIENDPLFPERINVNVASVVGPDHLKLRVWERGAGLTRACGTGACATAVAAIRRKLTGRKVRIDLP
GGPLTITWPEGGTIDMTGPATLSFEGAFEDSDFPA
>Mature_274_residues
PKSRGMRIPFRKMHGLGNDFVVIDAREAAVSMTAARAAALADRRTGIGCDQLIVLEPSDVADVRMRIFNNDGSEVEACGN
ASRAVGLLLGGTQFIETLGGLIRSEARPDGVSVDMGAPRLRWDEIPVSYAMDTLEMPVGWEDLENPVGVNVGNPHVIFFV
DDADAVDLARLGPLIENDPLFPERINVNVASVVGPDHLKLRVWERGAGLTRACGTGACATAVAAIRRKLTGRKVRIDLPG
GPLTITWPEGGTIDMTGPATLSFEGAFEDSDFPA

Specific function: Biosynthesis of lysine from aspartate semialdehyde; sixth step. [C]

COG id: COG0253

COG function: function code E; Diaminopimelate epimerase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the diaminopimelate epimerase family [H]

Homologues:

Organism=Escherichia coli, GI87082334, Length=271, Percent_Identity=40.590405904059, Blast_Score=181, Evalue=5e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001653
- InterPro:   IPR018510 [H]

Pfam domain/function: PF01678 DAP_epimerase [H]

EC number: =5.1.1.7 [H]

Molecular weight: Translated: 29375; Mature: 29244

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: PS01326 DAP_EPIMERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKSRGMRIPFRKMHGLGNDFVVIDAREAAVSMTAARAAALADRRTGIGCDQLIVLEPSD
CCCCCCCCCCHHHHHCCCCCEEEEECHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCC
VADVRMRIFNNDGSEVEACGNASRAVGLLLGGTQFIETLGGLIRSEARPDGVSVDMGAPR
CCEEEEEEECCCCCHHHHCCCCCCEEEEEECCHHHHHHHHHHHHCCCCCCCEEEECCCCC
LRWDEIPVSYAMDTLEMPVGWEDLENPVGVNVGNPHVIFFVDDADAVDLARLGPLIENDP
CEECCCCHHHHHHHHCCCCCHHHHCCCCCCCCCCCEEEEEECCCCCHHHHHHCCCCCCCC
LFPERINVNVASVVGPDHLKLRVWERGAGLTRACGTGACATAVAAIRRKLTGRKVRIDLP
CCCHHCCEEEEEEECCCCEEEEEEECCCCCCHHCCCCHHHHHHHHHHHHHCCCEEEEECC
GGPLTITWPEGGTIDMTGPATLSFEGAFEDSDFPA
CCCEEEECCCCCEEEECCCCEEEECCCCCCCCCCC
>Mature Secondary Structure 
PKSRGMRIPFRKMHGLGNDFVVIDAREAAVSMTAARAAALADRRTGIGCDQLIVLEPSD
CCCCCCCCCHHHHHCCCCCEEEEECHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCC
VADVRMRIFNNDGSEVEACGNASRAVGLLLGGTQFIETLGGLIRSEARPDGVSVDMGAPR
CCEEEEEEECCCCCHHHHCCCCCCEEEEEECCHHHHHHHHHHHHCCCCCCCEEEECCCCC
LRWDEIPVSYAMDTLEMPVGWEDLENPVGVNVGNPHVIFFVDDADAVDLARLGPLIENDP
CEECCCCHHHHHHHHCCCCCHHHHCCCCCCCCCCCEEEEEECCCCCHHHHHHCCCCCCCC
LFPERINVNVASVVGPDHLKLRVWERGAGLTRACGTGACATAVAAIRRKLTGRKVRIDLP
CCCHHCCEEEEEEECCCCEEEEEEECCCCCCHHCCCCHHHHHHHHHHHHHCCCEEEEECC
GGPLTITWPEGGTIDMTGPATLSFEGAFEDSDFPA
CCCEEEECCCCCEEEECCCCEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA