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The map label for this gene is ispB [H]

Identifier: 87199374

GI number: 87199374

Start: 1398395

End: 1399408

Strand: Direct

Name: ispB [H]

Synonym: Saro_1353

Alternate gene names: 87199374

Gene position: 1398395-1399408 (Clockwise)

Preceding gene: 87199372

Following gene: 87199375

Centisome position: 39.26

GC content: 65.58

Gene sequence:

>1014_bases
ATGACCGCGACCATCCATACCCTGCCGCGAAAGGCGGAACCCTCGCTTGCGCCGATGATGGCGCTGGTGGCTGAAGGCAT
GAACAGCGTCAACGCCGTGATTCTCGACAGGATGCAGTCGCGCATCCCGTTGATTCCGACGCTGGCGGGTCACCTCATCG
CCGGCGGTGGCAAGCGGATGCGCCCCATGCTCACGCTGGCGAGCGCGGCGCTGCTGGGCTACGGCGGCAGCCGCCATTAC
AAGCTCGCGGCGGCAGTCGAATTCATCCACACCGCAACGCTCCTGCATGACGACGTCGTCGATGGCTCCGACATGCGTCG
CGGCAAGAAGACCGCGAATATCGTCTACGGCAACCCGGCGACGGTCCTTGTCGGCGACTTCCTGTTCAGCCGCAGCTTCG
AACTCATGGTCGAGGACGGCTCGCTCAAGGTCCTGCGCATCCTGTCGAACGCATCGGCGGTCATCTCCGAAGGGGAGGTC
GACCAGCTCGTCGCCCAGCGCCAGATCGACACGACCGAGGAAATGTACCTCGATATCATTGCCGCCAAGACTGCCGCGCT
GTTTTCGGCCGCCTGCCGCATCGCGGCCGTCGTCGCCGAGCGGCCCGATGCCGACGAACTCGCGCTCGATGCCTATGGCC
GCAATCTCGGCATTGCCTTCCAGCTTGCCGACGATGCCATCGACTACGATTCCGATGCCGCCGAAATGGGCAAGGACCAG
GGCGACGATTTCCGCGAGGGCAAGATGACGCTCCCGGTGATCCTCGCCTACGCCCGCGGCGACGAGGAAGAACGCGGGTT
CTGGCAGCAGGCCATCGCGGGAGAGCGTTCCTCCGACGCGGACCTGGCCCATGCCATTTCCCTTATCCGGCGGCACGATG
CGGTCCATGCGACGCGCGAACGCGCCCGGATGTATGCGCAGCGCGCCATCGACGCGATCTCGGGCTTCCCGGCAAGTGCG
GCGAAGGCGGCAATGACGGAAGCGGCCGAGTTCGCCGTCGCCCGGAGATTCTGA

Upstream 100 bases:

>100_bases
CTTCGCTCATTCCCTTACCCCGTCATTTGGTCGAACCGCGCGCTAACGTGCAACTTGCGTCTTGCCAAGCGCCGCCCTTG
CGGCCAAGCGGTCAGCCACG

Downstream 100 bases:

>100_bases
AGCCCCCCTCGAACAGATAGGTTGGACCCTGATGCAGTATCGTCGCCTCGGCCGCTCGGCCATCGTCGTCTCGGACATCT
GCATGGGGACGATGACCTTC

Product: farnesyltranstransferase

Products: NA

Alternate protein names: All-trans-octaprenyl-diphosphate synthase; Octaprenyl pyrophosphate synthase; OPP synthase [H]

Number of amino acids: Translated: 337; Mature: 336

Protein sequence:

>337_residues
MTATIHTLPRKAEPSLAPMMALVAEGMNSVNAVILDRMQSRIPLIPTLAGHLIAGGGKRMRPMLTLASAALLGYGGSRHY
KLAAAVEFIHTATLLHDDVVDGSDMRRGKKTANIVYGNPATVLVGDFLFSRSFELMVEDGSLKVLRILSNASAVISEGEV
DQLVAQRQIDTTEEMYLDIIAAKTAALFSAACRIAAVVAERPDADELALDAYGRNLGIAFQLADDAIDYDSDAAEMGKDQ
GDDFREGKMTLPVILAYARGDEEERGFWQQAIAGERSSDADLAHAISLIRRHDAVHATRERARMYAQRAIDAISGFPASA
AKAAMTEAAEFAVARRF

Sequences:

>Translated_337_residues
MTATIHTLPRKAEPSLAPMMALVAEGMNSVNAVILDRMQSRIPLIPTLAGHLIAGGGKRMRPMLTLASAALLGYGGSRHY
KLAAAVEFIHTATLLHDDVVDGSDMRRGKKTANIVYGNPATVLVGDFLFSRSFELMVEDGSLKVLRILSNASAVISEGEV
DQLVAQRQIDTTEEMYLDIIAAKTAALFSAACRIAAVVAERPDADELALDAYGRNLGIAFQLADDAIDYDSDAAEMGKDQ
GDDFREGKMTLPVILAYARGDEEERGFWQQAIAGERSSDADLAHAISLIRRHDAVHATRERARMYAQRAIDAISGFPASA
AKAAMTEAAEFAVARRF
>Mature_336_residues
TATIHTLPRKAEPSLAPMMALVAEGMNSVNAVILDRMQSRIPLIPTLAGHLIAGGGKRMRPMLTLASAALLGYGGSRHYK
LAAAVEFIHTATLLHDDVVDGSDMRRGKKTANIVYGNPATVLVGDFLFSRSFELMVEDGSLKVLRILSNASAVISEGEVD
QLVAQRQIDTTEEMYLDIIAAKTAALFSAACRIAAVVAERPDADELALDAYGRNLGIAFQLADDAIDYDSDAAEMGKDQG
DDFREGKMTLPVILAYARGDEEERGFWQQAIAGERSSDADLAHAISLIRRHDAVHATRERARMYAQRAIDAISGFPASAA
KAAMTEAAEFAVARRF

Specific function: Supplies octaprenyl diphosphate, the precursor for the side chain of the isoprenoid quinones ubiquinone and menaquinone [H]

COG id: COG0142

COG function: function code H; Geranylgeranyl pyrophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FPP/GGPP synthase family [H]

Homologues:

Organism=Homo sapiens, GI50659086, Length=290, Percent_Identity=32.4137931034483, Blast_Score=136, Evalue=2e-32,
Organism=Homo sapiens, GI4758430, Length=288, Percent_Identity=23.9583333333333, Blast_Score=77, Evalue=3e-14,
Organism=Homo sapiens, GI83700220, Length=288, Percent_Identity=23.9583333333333, Blast_Score=77, Evalue=3e-14,
Organism=Escherichia coli, GI1789578, Length=322, Percent_Identity=44.4099378881988, Blast_Score=261, Evalue=3e-71,
Organism=Escherichia coli, GI1786623, Length=240, Percent_Identity=32.5, Blast_Score=88, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17505681, Length=246, Percent_Identity=34.5528455284553, Blast_Score=141, Evalue=6e-34,
Organism=Saccharomyces cerevisiae, GI6319475, Length=305, Percent_Identity=31.1475409836066, Blast_Score=131, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6325188, Length=254, Percent_Identity=23.6220472440945, Blast_Score=83, Evalue=5e-17,
Organism=Drosophila melanogaster, GI24651612, Length=302, Percent_Identity=29.1390728476821, Blast_Score=129, Evalue=3e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000092
- InterPro:   IPR017446
- InterPro:   IPR008949 [H]

Pfam domain/function: PF00348 polyprenyl_synt [H]

EC number: =2.5.1.90 [H]

Molecular weight: Translated: 36337; Mature: 36206

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: PS00723 POLYPRENYL_SYNTHET_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTATIHTLPRKAEPSLAPMMALVAEGMNSVNAVILDRMQSRIPLIPTLAGHLIAGGGKRM
CCCCCCCCCCCCCCCHHHHHHHHHHCHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHH
RPMLTLASAALLGYGGSRHYKLAAAVEFIHTATLLHDDVVDGSDMRRGKKTANIVYGNPA
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCEEEECCCH
TVLVGDFLFSRSFELMVEDGSLKVLRILSNASAVISEGEVDQLVAQRQIDTTEEMYLDII
HHHHHHHHHCCCEEEEEECCCEEEHHHHHCCHHHHCCCHHHHHHHHHHCCCHHHHHHHHH
AAKTAALFSAACRIAAVVAERPDADELALDAYGRNLGIAFQLADDAIDYDSDAAEMGKDQ
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCEEEEEEECCCCCCCCCHHHCCCCC
GDDFREGKMTLPVILAYARGDEEERGFWQQAIAGERSSDADLAHAISLIRRHDAVHATRE
CCCHHCCCEEEHHEEEECCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHH
RARMYAQRAIDAISGFPASAAKAAMTEAAEFAVARRF
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TATIHTLPRKAEPSLAPMMALVAEGMNSVNAVILDRMQSRIPLIPTLAGHLIAGGGKRM
CCCCCCCCCCCCCCHHHHHHHHHHCHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHH
RPMLTLASAALLGYGGSRHYKLAAAVEFIHTATLLHDDVVDGSDMRRGKKTANIVYGNPA
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCEEEECCCH
TVLVGDFLFSRSFELMVEDGSLKVLRILSNASAVISEGEVDQLVAQRQIDTTEEMYLDII
HHHHHHHHHCCCEEEEEECCCEEEHHHHHCCHHHHCCCHHHHHHHHHHCCCHHHHHHHHH
AAKTAALFSAACRIAAVVAERPDADELALDAYGRNLGIAFQLADDAIDYDSDAAEMGKDQ
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCEEEEEEECCCCCCCCCHHHCCCCC
GDDFREGKMTLPVILAYARGDEEERGFWQQAIAGERSSDADLAHAISLIRRHDAVHATRE
CCCHHCCCEEEHHEEEECCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHH
RARMYAQRAIDAISGFPASAAKAAMTEAAEFAVARRF
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8312607; 9278503; 2670911; 8037730 [H]