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The map label for this gene is aspS

Identifier: 87199359

GI number: 87199359

Start: 1379139

End: 1380920

Strand: Direct

Name: aspS

Synonym: Saro_1338

Alternate gene names: 87199359

Gene position: 1379139-1380920 (Clockwise)

Preceding gene: 87199356

Following gene: 87199360

Centisome position: 38.72

GC content: 65.88

Gene sequence:

>1782_bases
ATGCACCTTTATCGTTCCCATACCTGCGGCGCCCTCTCGCGCGGTGATGTCGGGCAGACTGTCCGCCTTTCGGGCTGGGT
GCACCGCAAGCGCGACCATGGCGGCGTGCTGTTCGTCGACCTGCGCGACCACTATGGCATGACCCAGATCGTGGCCGACG
CCGACAGTCCGGCGCTGCCGATCCTCGAGGCCCTGCGCGTGGAATCGGTGGTGACCATCGATGGCGAGGTGAAGGCGCGT
TCGGAGGGCACGGTGAACGCCAACCTCTCGACCGGCGAGATCGAGGTCTTCGCGCGCGTCGCGACGGTGCTTTCGGCGGC
CGAGGAGCTGCCGATGCCGGTCGCGGGCGAGCAGGAATATCCCGAGGACATCCGCCTGCGCTATCGCTTCCTCGACCTGC
GCCGCGAGACGCTGCACGCGAACATCGTCAAGCGCACCAAGGTTATCTCGGACATGCGCCGCCGCATGGAAGGGGCGGGC
TTCACCGAATACTCGACCCCGATCCTCACCGCATCCTCGCCCGAGGGCGCGCGCGACTTCCTCGTGCCGAGCCGCATCCA
TCCCGGCAAGTTCTATGCGCTGCCGCAGGCACCGCAGCAGTACAAACAGCTCCTGATGGTCGCGGGCTTCGACCGCTACT
TCCAGATCGCGCCGTGCTTCCGCGACGAGGACCCGCGCGCCGACCGCCTGCCGGGCGAATTCTACCAGCTCGACCTCGAG
ATGAGCTTCGTCACCCAGGAAGAAGTCTGGGAGACGATGGAGCCGGTGATCGGCGGCGTGTTCGAAGCCTTTGCCGATGG
CCGCAAGGTCACTCCCATCGGCAGCTTCCCTCGCATTCCCTATGCCGAGGCGATGCTCAAGTACGGTTCGGACAAGCCGG
ACCTGCGCAACCCCATCATCATCTCCGACGTTTCGGAAGAGTTCGCCCAGTCGGGCTTCGGGCTGTTCGAGAAGATCGTT
GGCACCGGCGGCGTCGTGCGCCTGATCCCGGCGCCGAACACCGCGGACAAGAGCCGCAAGTTCTTCGACGAGATGAACGA
CTGGGCGCGCGCGGAAGGCCATGCCGGACTTGGCTACGTCACGCGCAAGCAGGGCGAGTTCGGCGGCCCGATCGCCAAGA
ACCATGGCGCGGACAAGATGGCTGCGCTGTTCGACCGGCTGGGCCTCGGCCCTGATGACGGCTGCTTCTTCGCGGCGGGC
AAGGCCGAGCAGGCCGCCAAGCTGGCAGGCGCGGCGCGCACCCGCGTGGCGGACCAGCTTGGCCTGATCGACAAGGACCG
CTTCGAGCTGTGCTGGATCGTCGACTTCCCGTTCTACGAATGGGACGAAGAGAACAAGAAGGTAGAGTTCAGCCACAACC
CGTTCTCGATGCCGCAGGGCGGGCTGGACGCGTTGAATACGCAGGACCCGCTGACGATCAATGCCTTCCAGTACGATCTC
GTCTGCAACGGCTTCGAGATCGCCTCGGGCTCGATCCGCAACCAGAGCCCCGAGACGATGGTCAAGGCATTCGAGATCGT
CGGCCTTTCCAAGGCGGACGTCGAGGAGCGCTTCGGCGGCCTTTACCGCGCGTTCCAGTACGGCGCGCCGCCGCACGGCG
GCATGGCGGCGGGCGTGGATCGCATCGTCATGCTGATCTGCGGAGCGCAGAACCTGCGCGAGATCACGCTGTTCCCGATG
AACCAGCGCGCCGAGGACCTGCTGATGGGCGCGCCTTCGCCGGCGGCGCTCAAGCAACTGCGCGAGCTGAACATTCGCGT
GGTCGAACAGACCAAGGGGTGA

Upstream 100 bases:

>100_bases
TCCTTGGTTGGCCGGGGGCGTGGCTTGACAAATCCGCGGCCATCGCCTGTTAGCCGCGAACTTCCCCTGCCCGATTGCAG
ACCGATTGGAAAGAGTTTCG

Downstream 100 bases:

>100_bases
GATGGACGGGGCGCGCGCCAGGGTACACGGCAAGGTGATCCACAGCGCGCCTCTTGCCAGCGCCCTTTGCGTTCATTAGG
GCGAAACCCGAACAGACAAC

Product: aspartyl-tRNA synthetase

Products: NA

Alternate protein names: Aspartate--tRNA ligase; AspRS

Number of amino acids: Translated: 593; Mature: 593

Protein sequence:

>593_residues
MHLYRSHTCGALSRGDVGQTVRLSGWVHRKRDHGGVLFVDLRDHYGMTQIVADADSPALPILEALRVESVVTIDGEVKAR
SEGTVNANLSTGEIEVFARVATVLSAAEELPMPVAGEQEYPEDIRLRYRFLDLRRETLHANIVKRTKVISDMRRRMEGAG
FTEYSTPILTASSPEGARDFLVPSRIHPGKFYALPQAPQQYKQLLMVAGFDRYFQIAPCFRDEDPRADRLPGEFYQLDLE
MSFVTQEEVWETMEPVIGGVFEAFADGRKVTPIGSFPRIPYAEAMLKYGSDKPDLRNPIIISDVSEEFAQSGFGLFEKIV
GTGGVVRLIPAPNTADKSRKFFDEMNDWARAEGHAGLGYVTRKQGEFGGPIAKNHGADKMAALFDRLGLGPDDGCFFAAG
KAEQAAKLAGAARTRVADQLGLIDKDRFELCWIVDFPFYEWDEENKKVEFSHNPFSMPQGGLDALNTQDPLTINAFQYDL
VCNGFEIASGSIRNQSPETMVKAFEIVGLSKADVEERFGGLYRAFQYGAPPHGGMAAGVDRIVMLICGAQNLREITLFPM
NQRAEDLLMGAPSPAALKQLRELNIRVVEQTKG

Sequences:

>Translated_593_residues
MHLYRSHTCGALSRGDVGQTVRLSGWVHRKRDHGGVLFVDLRDHYGMTQIVADADSPALPILEALRVESVVTIDGEVKAR
SEGTVNANLSTGEIEVFARVATVLSAAEELPMPVAGEQEYPEDIRLRYRFLDLRRETLHANIVKRTKVISDMRRRMEGAG
FTEYSTPILTASSPEGARDFLVPSRIHPGKFYALPQAPQQYKQLLMVAGFDRYFQIAPCFRDEDPRADRLPGEFYQLDLE
MSFVTQEEVWETMEPVIGGVFEAFADGRKVTPIGSFPRIPYAEAMLKYGSDKPDLRNPIIISDVSEEFAQSGFGLFEKIV
GTGGVVRLIPAPNTADKSRKFFDEMNDWARAEGHAGLGYVTRKQGEFGGPIAKNHGADKMAALFDRLGLGPDDGCFFAAG
KAEQAAKLAGAARTRVADQLGLIDKDRFELCWIVDFPFYEWDEENKKVEFSHNPFSMPQGGLDALNTQDPLTINAFQYDL
VCNGFEIASGSIRNQSPETMVKAFEIVGLSKADVEERFGGLYRAFQYGAPPHGGMAAGVDRIVMLICGAQNLREITLFPM
NQRAEDLLMGAPSPAALKQLRELNIRVVEQTKG
>Mature_593_residues
MHLYRSHTCGALSRGDVGQTVRLSGWVHRKRDHGGVLFVDLRDHYGMTQIVADADSPALPILEALRVESVVTIDGEVKAR
SEGTVNANLSTGEIEVFARVATVLSAAEELPMPVAGEQEYPEDIRLRYRFLDLRRETLHANIVKRTKVISDMRRRMEGAG
FTEYSTPILTASSPEGARDFLVPSRIHPGKFYALPQAPQQYKQLLMVAGFDRYFQIAPCFRDEDPRADRLPGEFYQLDLE
MSFVTQEEVWETMEPVIGGVFEAFADGRKVTPIGSFPRIPYAEAMLKYGSDKPDLRNPIIISDVSEEFAQSGFGLFEKIV
GTGGVVRLIPAPNTADKSRKFFDEMNDWARAEGHAGLGYVTRKQGEFGGPIAKNHGADKMAALFDRLGLGPDDGCFFAAG
KAEQAAKLAGAARTRVADQLGLIDKDRFELCWIVDFPFYEWDEENKKVEFSHNPFSMPQGGLDALNTQDPLTINAFQYDL
VCNGFEIASGSIRNQSPETMVKAFEIVGLSKADVEERFGGLYRAFQYGAPPHGGMAAGVDRIVMLICGAQNLREITLFPM
NQRAEDLLMGAPSPAALKQLRELNIRVVEQTKG

Specific function: Unknown

COG id: COG0173

COG function: function code J; Aspartyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI40789249, Length=600, Percent_Identity=35.6666666666667, Blast_Score=348, Evalue=6e-96,
Organism=Homo sapiens, GI45439306, Length=268, Percent_Identity=27.2388059701493, Blast_Score=90, Evalue=6e-18,
Organism=Homo sapiens, GI226491502, Length=256, Percent_Identity=23.828125, Blast_Score=72, Evalue=2e-12,
Organism=Homo sapiens, GI5031815, Length=262, Percent_Identity=25.5725190839695, Blast_Score=71, Evalue=3e-12,
Organism=Homo sapiens, GI194272210, Length=262, Percent_Identity=25.5725190839695, Blast_Score=71, Evalue=3e-12,
Organism=Escherichia coli, GI1788173, Length=596, Percent_Identity=40.4362416107383, Blast_Score=438, Evalue=1e-124,
Organism=Escherichia coli, GI1790571, Length=322, Percent_Identity=28.5714285714286, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI1789256, Length=323, Percent_Identity=28.4829721362229, Blast_Score=66, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI32566633, Length=605, Percent_Identity=33.7190082644628, Blast_Score=294, Evalue=7e-80,
Organism=Caenorhabditis elegans, GI17551876, Length=298, Percent_Identity=26.8456375838926, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI71984122, Length=303, Percent_Identity=26.0726072607261, Blast_Score=70, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI71994340, Length=341, Percent_Identity=23.7536656891496, Blast_Score=70, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17535927, Length=341, Percent_Identity=23.7536656891496, Blast_Score=69, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI17535925, Length=341, Percent_Identity=23.7536656891496, Blast_Score=69, Evalue=7e-12,
Organism=Saccharomyces cerevisiae, GI6325153, Length=661, Percent_Identity=29.6520423600605, Blast_Score=233, Evalue=5e-62,
Organism=Saccharomyces cerevisiae, GI6323011, Length=266, Percent_Identity=30.0751879699248, Blast_Score=89, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6321807, Length=245, Percent_Identity=26.9387755102041, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24584738, Length=606, Percent_Identity=33.4983498349835, Blast_Score=296, Evalue=2e-80,
Organism=Drosophila melanogaster, GI17136276, Length=282, Percent_Identity=27.3049645390071, Blast_Score=81, Evalue=2e-15,

Paralogues:

None

Copy number: 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYD_NOVAD (Q2G8P1)

Other databases:

- EMBL:   CP000248
- RefSeq:   YP_496616.1
- ProteinModelPortal:   Q2G8P1
- SMR:   Q2G8P1
- STRING:   Q2G8P1
- GeneID:   3917788
- GenomeReviews:   CP000248_GR
- KEGG:   nar:Saro_1338
- NMPDR:   fig|48935.1.peg.1849
- eggNOG:   COG0173
- HOGENOM:   HBG396032
- OMA:   YQLDVEM
- ProtClustDB:   PRK00476
- BioCyc:   NARO279238:SARO_1338-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00044_B
- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR020564
- InterPro:   IPR004524
- InterPro:   IPR018153
- InterPro:   IPR002312
- InterPro:   IPR004115
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365
- Gene3D:   G3DSA:3.30.1360.30
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR22594
- PANTHER:   PTHR22594:SF5
- PRINTS:   PR01042
- TIGRFAMs:   TIGR00459

Pfam domain/function: PF02938 GAD; PF00152 tRNA-synt_2; PF01336 tRNA_anti; SSF50249 Nucleic_acid_OB

EC number: =6.1.1.12

Molecular weight: Translated: 65746; Mature: 65746

Theoretical pI: Translated: 5.17; Mature: 5.17

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHLYRSHTCGALSRGDVGQTVRLSGWVHRKRDHGGVLFVDLRDHYGMTQIVADADSPALP
CCCCCCCCCCCCCCCCCCCEEEECCEEEEECCCCCEEEEEECCCCCCEEEEECCCCCCHH
ILEALRVESVVTIDGEVKARSEGTVNANLSTGEIEVFARVATVLSAAEELPMPVAGEQEY
HHHHHHHCEEEEECCCEEECCCCEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCC
PEDIRLRYRFLDLRRETLHANIVKRTKVISDMRRRMEGAGFTEYSTPILTASSPEGARDF
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCCCHHC
LVPSRIHPGKFYALPQAPQQYKQLLMVAGFDRYFQIAPCFRDEDPRADRLPGEFYQLDLE
CCCCCCCCCCEEECCCCHHHHHHHHHHHCCCHHEEECCCCCCCCCCCCCCCCCEEEEEEE
MSFVTQEEVWETMEPVIGGVFEAFADGRKVTPIGSFPRIPYAEAMLKYGSDKPDLRNPII
EEECCHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCCHHHHHHHCCCCCCCCCCCEE
ISDVSEEFAQSGFGLFEKIVGTGGVVRLIPAPNTADKSRKFFDEMNDWARAEGHAGLGYV
EECCHHHHHHCCHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCEE
TRKQGEFGGPIAKNHGADKMAALFDRLGLGPDDGCFFAAGKAEQAAKLAGAARTRVADQL
EECCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHH
GLIDKDRFELCWIVDFPFYEWDEENKKVEFSHNPFSMPQGGLDALNTQDPLTINAFQYDL
CCCCCCCEEEEEEEECCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCEEEEEEEEEE
VCNGFEIASGSIRNQSPETMVKAFEIVGLSKADVEERFGGLYRAFQYGAPPHGGMAAGVD
EECCEEECCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHH
RIVMLICGAQNLREITLFPMNQRAEDLLMGAPSPAALKQLRELNIRVVEQTKG
HHHHHHHCCCCCEEEEEEECCCCHHHHHCCCCCHHHHHHHHHCCEEEEEECCC
>Mature Secondary Structure
MHLYRSHTCGALSRGDVGQTVRLSGWVHRKRDHGGVLFVDLRDHYGMTQIVADADSPALP
CCCCCCCCCCCCCCCCCCCEEEECCEEEEECCCCCEEEEEECCCCCCEEEEECCCCCCHH
ILEALRVESVVTIDGEVKARSEGTVNANLSTGEIEVFARVATVLSAAEELPMPVAGEQEY
HHHHHHHCEEEEECCCEEECCCCEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCC
PEDIRLRYRFLDLRRETLHANIVKRTKVISDMRRRMEGAGFTEYSTPILTASSPEGARDF
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCCCHHC
LVPSRIHPGKFYALPQAPQQYKQLLMVAGFDRYFQIAPCFRDEDPRADRLPGEFYQLDLE
CCCCCCCCCCEEECCCCHHHHHHHHHHHCCCHHEEECCCCCCCCCCCCCCCCCEEEEEEE
MSFVTQEEVWETMEPVIGGVFEAFADGRKVTPIGSFPRIPYAEAMLKYGSDKPDLRNPII
EEECCHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCCHHHHHHHCCCCCCCCCCCEE
ISDVSEEFAQSGFGLFEKIVGTGGVVRLIPAPNTADKSRKFFDEMNDWARAEGHAGLGYV
EECCHHHHHHCCHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCEE
TRKQGEFGGPIAKNHGADKMAALFDRLGLGPDDGCFFAAGKAEQAAKLAGAARTRVADQL
EECCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHH
GLIDKDRFELCWIVDFPFYEWDEENKKVEFSHNPFSMPQGGLDALNTQDPLTINAFQYDL
CCCCCCCEEEEEEEECCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCEEEEEEEEEE
VCNGFEIASGSIRNQSPETMVKAFEIVGLSKADVEERFGGLYRAFQYGAPPHGGMAAGVD
EECCEEECCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHH
RIVMLICGAQNLREITLFPMNQRAEDLLMGAPSPAALKQLRELNIRVVEQTKG
HHHHHHHCCCCCEEEEEEECCCCHHHHHCCCCCHHHHHHHHHCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA