Click here to switch to the map view.

The map label for this gene is trmL [H]

Identifier: 87199225

GI number: 87199225

Start: 1251369

End: 1251824

Strand: Direct

Name: trmL [H]

Synonym: Saro_1203

Alternate gene names: 87199225

Gene position: 1251369-1251824 (Clockwise)

Preceding gene: 87199224

Following gene: 87199228

Centisome position: 35.14

GC content: 67.76

Gene sequence:

>456_bases
ATGCGGATTGCCCTGTACGAGCCGGAGATTGCCGGAAACGTTGGCGCGGTCATGCGCCTCGGTGCGTGCCTTGGCGTTAA
TGTCGACCTGGTGGAGCCGATGGGCTTCGCTTGGGAGGATCGGCGCGTGCGGCGCGCGGCGATGGACTACATCGACCACG
TGAAGGTCACGCGCCACGCCGGCTTCGGGCCGTTTCGCGCCTCTGTCGCGCCTGGGCGGCTGGTTCTGTTCACCACGAAG
GCCGAACAGTCGCTACACGACTTCCGCTTCAGGGCCGATGACGTGCTGCTGTTCGGCAAGGAGAGCGCGGGCGTTCCGGA
CGAGGTGGCGCAGGCCTGTGACGAGCGGGTGCGGATCCCGATCCGGCCGGAAGTGCGCTCGCTGAACCTCGCGATTGCAA
CGGCCCTGGCGCTGGGTGAGGCCCTTCGCCAGACAGGAGGGCTGCCGCCGGGGTAA

Upstream 100 bases:

>100_bases
TGCAGCTTCTCGCCTACCACGTGGCCGTGGCCAAGGGCACCGACGTCGACCAGCCGCGCAACCTTGCCAAGAGCGTGACG
GTGGAGTGAGCCCGGCGGCG

Downstream 100 bases:

>100_bases
TCGGGCGGGGACTGCAAGGAAATGGTCGGGGAGATAGGATTCGAACCTACGACCCTCTGGTCCCAAACCAGATGCGCTAC
CAGGCTGCGCTACTCCCCGA

Product: tRNA/rRNA methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 151; Mature: 151

Protein sequence:

>151_residues
MRIALYEPEIAGNVGAVMRLGACLGVNVDLVEPMGFAWEDRRVRRAAMDYIDHVKVTRHAGFGPFRASVAPGRLVLFTTK
AEQSLHDFRFRADDVLLFGKESAGVPDEVAQACDERVRIPIRPEVRSLNLAIATALALGEALRQTGGLPPG

Sequences:

>Translated_151_residues
MRIALYEPEIAGNVGAVMRLGACLGVNVDLVEPMGFAWEDRRVRRAAMDYIDHVKVTRHAGFGPFRASVAPGRLVLFTTK
AEQSLHDFRFRADDVLLFGKESAGVPDEVAQACDERVRIPIRPEVRSLNLAIATALALGEALRQTGGLPPG
>Mature_151_residues
MRIALYEPEIAGNVGAVMRLGACLGVNVDLVEPMGFAWEDRRVRRAAMDYIDHVKVTRHAGFGPFRASVAPGRLVLFTTK
AEQSLHDFRFRADDVLLFGKESAGVPDEVAQACDERVRIPIRPEVRSLNLAIATALALGEALRQTGGLPPG

Specific function: Methylates the ribose at the nucleotide 34 wobble position in the two leucyl isoacceptors tRNA(Leu)(CmAA) and tRNA(Leu)(cmnm5UmAA). Catalyzes the methyl transfer from S- adenosyl-L-methionine to the 2'-OH of the wobble nucleotide [H]

COG id: COG0219

COG function: function code J; Predicted rRNA methylase (SpoU class)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. TrmL subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790034, Length=148, Percent_Identity=40.5405405405405, Blast_Score=123, Evalue=4e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016914
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 16452; Mature: 16452

Theoretical pI: Translated: 7.52; Mature: 7.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIALYEPEIAGNVGAVMRLGACLGVNVDLVEPMGFAWEDRRVRRAAMDYIDHVKVTRHA
CEEEEECCCCCCCHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCC
GFGPFRASVAPGRLVLFTTKAEQSLHDFRFRADDVLLFGKESAGVPDEVAQACDERVRIP
CCCCCCCCCCCCEEEEEECCCHHHHHHHHCCCCCEEEECCCCCCCCHHHHHHHHHHCCCC
IRPEVRSLNLAIATALALGEALRQTGGLPPG
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MRIALYEPEIAGNVGAVMRLGACLGVNVDLVEPMGFAWEDRRVRRAAMDYIDHVKVTRHA
CEEEEECCCCCCCHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCC
GFGPFRASVAPGRLVLFTTKAEQSLHDFRFRADDVLLFGKESAGVPDEVAQACDERVRIP
CCCCCCCCCCCCEEEEEECCCHHHHHHHHCCCCCEEEECCCCCCCCHHHHHHHHHHCCCC
IRPEVRSLNLAIATALALGEALRQTGGLPPG
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA