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The map label for this gene is mdh

Identifier: 87199204

GI number: 87199204

Start: 1223941

End: 1224903

Strand: Reverse

Name: mdh

Synonym: Saro_1182

Alternate gene names: 87199204

Gene position: 1224903-1223941 (Counterclockwise)

Preceding gene: 87199205

Following gene: 87199203

Centisome position: 34.39

GC content: 65.42

Gene sequence:

>963_bases
ATGGCACGCAAGAAGATCGCGCTGATCGGCGCGGGCAACATCGGTGGCACTCTTGCCCACCTCGCCGCCCAGAAGGAACT
TGGCGACATCGTCCTGTTCGACGTCGTTGAAGGCGTGCCCCAGGGCAAGGCGCTCGACCTTTCGCAGTGCGGTCCGGTCG
AAGGCTTCGACGCCAACATCATCGGCACCAACGACTACAAGGGCATCGCAGGCGCGGACGTGATCATCGTCACCGCCGGC
GTCGCCCGCAAGCCCGGCATGAGCCGCGACGACCTGCTCGGCATCAACCTCAAGGTGATGAAGGCCGTCGGCGAAGGCAT
CCGCGACAACGCGCCCGACGCGTTCGTGATCTGCATCACCAACCCGCTCGACGCGATGGTCTGGGCGCTGCGCGAGTTCT
CGGGCCTGCCCGCGAACAAGGTCGTCGGCATGGCCGGCGTGCTCGACTCGGCGCGCTTCAGCACGTTCCTGGCATGGGAA
TTCGGCGTCTCGATCCGCGACGTGAACACGTTCGTTCTCGGCGGCCACGGCGACACCATGGTTCCGGTCACCCAGTACTC
GACCGTCAACGGCATCCCGGTGCCCGACCTCGTCAAGATGGGCCTGTCCACCCAGGAAAAGATCGACGCGATCGTGCAGC
GCACCCGTTCGGGCGGCGGCGAGATCGTCGGGCTGCTCAAGACCGGTTCGGCGTTCTACGCGCCTGCCGCATCGGGCATC
GCGATGGCCGAAGCCTATCTCAACGACCAGAAGCGCATCCTGCCCTGCGCCGCCTACGTTGACGGCGAATACGGCGTGAA
CGGTCTTTACGTCGGCGTGCCGGTGCTGATCGGCGCGAACGGCGTCGAGAAGGTGATCGAGATCGAACTCGACGACGAAG
CCAAGGGCAACCTCCAGGTCTCCGTCGACGCGGTCAAGGAACTGCTGGAAGCCTGCAAGGGCATCGACCCCAGCCTCGCC
TGA

Upstream 100 bases:

>100_bases
CAGCACTTCCCTGTCTCGCGGGGCACGGGGAGGAACTGGGGCCGGTTGCCCGCCGGTTGTCTGTATGCTTGTCCCCGCGA
ACGGGAAAGGACCGTAAAGC

Downstream 100 bases:

>100_bases
TTTTCTGACACGATTTGACAGTTGCGCCCCATCCTCCCCTGGGGCCACCTGACTGCCGAGCCGCCCGTCCCGCAATCCAG
ACGCGACGGGTGGCAGAACC

Product: malate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 320; Mature: 319

Protein sequence:

>320_residues
MARKKIALIGAGNIGGTLAHLAAQKELGDIVLFDVVEGVPQGKALDLSQCGPVEGFDANIIGTNDYKGIAGADVIIVTAG
VARKPGMSRDDLLGINLKVMKAVGEGIRDNAPDAFVICITNPLDAMVWALREFSGLPANKVVGMAGVLDSARFSTFLAWE
FGVSIRDVNTFVLGGHGDTMVPVTQYSTVNGIPVPDLVKMGLSTQEKIDAIVQRTRSGGGEIVGLLKTGSAFYAPAASGI
AMAEAYLNDQKRILPCAAYVDGEYGVNGLYVGVPVLIGANGVEKVIEIELDDEAKGNLQVSVDAVKELLEACKGIDPSLA

Sequences:

>Translated_320_residues
MARKKIALIGAGNIGGTLAHLAAQKELGDIVLFDVVEGVPQGKALDLSQCGPVEGFDANIIGTNDYKGIAGADVIIVTAG
VARKPGMSRDDLLGINLKVMKAVGEGIRDNAPDAFVICITNPLDAMVWALREFSGLPANKVVGMAGVLDSARFSTFLAWE
FGVSIRDVNTFVLGGHGDTMVPVTQYSTVNGIPVPDLVKMGLSTQEKIDAIVQRTRSGGGEIVGLLKTGSAFYAPAASGI
AMAEAYLNDQKRILPCAAYVDGEYGVNGLYVGVPVLIGANGVEKVIEIELDDEAKGNLQVSVDAVKELLEACKGIDPSLA
>Mature_319_residues
ARKKIALIGAGNIGGTLAHLAAQKELGDIVLFDVVEGVPQGKALDLSQCGPVEGFDANIIGTNDYKGIAGADVIIVTAGV
ARKPGMSRDDLLGINLKVMKAVGEGIRDNAPDAFVICITNPLDAMVWALREFSGLPANKVVGMAGVLDSARFSTFLAWEF
GVSIRDVNTFVLGGHGDTMVPVTQYSTVNGIPVPDLVKMGLSTQEKIDAIVQRTRSGGGEIVGLLKTGSAFYAPAASGIA
MAEAYLNDQKRILPCAAYVDGEYGVNGLYVGVPVLIGANGVEKVIEIELDDEAKGNLQVSVDAVKELLEACKGIDPSLA

Specific function: Catalyzes the reversible oxidation of malate to oxaloacetate

COG id: COG0039

COG function: function code C; Malate/lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH/MDH superfamily. MDH type 3 family

Homologues:

Organism=Homo sapiens, GI47059044, Length=317, Percent_Identity=30.2839116719243, Blast_Score=157, Evalue=1e-38,
Organism=Homo sapiens, GI221136809, Length=317, Percent_Identity=30.2839116719243, Blast_Score=157, Evalue=1e-38,
Organism=Homo sapiens, GI15082234, Length=315, Percent_Identity=26.984126984127, Blast_Score=138, Evalue=6e-33,
Organism=Homo sapiens, GI291575128, Length=315, Percent_Identity=29.8412698412698, Blast_Score=137, Evalue=1e-32,
Organism=Homo sapiens, GI4557032, Length=315, Percent_Identity=29.8412698412698, Blast_Score=137, Evalue=1e-32,
Organism=Homo sapiens, GI5031857, Length=310, Percent_Identity=28.3870967741935, Blast_Score=135, Evalue=4e-32,
Organism=Homo sapiens, GI260099723, Length=310, Percent_Identity=28.3870967741935, Blast_Score=135, Evalue=4e-32,
Organism=Homo sapiens, GI9257228, Length=308, Percent_Identity=29.2207792207792, Blast_Score=132, Evalue=4e-31,
Organism=Homo sapiens, GI4504973, Length=308, Percent_Identity=29.2207792207792, Blast_Score=132, Evalue=4e-31,
Organism=Homo sapiens, GI260099725, Length=200, Percent_Identity=32, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI260099727, Length=200, Percent_Identity=32, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI21735621, Length=296, Percent_Identity=28.7162162162162, Blast_Score=92, Evalue=7e-19,
Organism=Homo sapiens, GI207028494, Length=193, Percent_Identity=27.4611398963731, Blast_Score=85, Evalue=7e-17,
Organism=Homo sapiens, GI103472011, Length=313, Percent_Identity=22.3642172523962, Blast_Score=75, Evalue=6e-14,
Organism=Escherichia coli, GI1789632, Length=296, Percent_Identity=31.7567567567568, Blast_Score=100, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17535107, Length=296, Percent_Identity=26.6891891891892, Blast_Score=109, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17554310, Length=297, Percent_Identity=34.3434343434343, Blast_Score=102, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI17561064, Length=300, Percent_Identity=27, Blast_Score=69, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6322765, Length=299, Percent_Identity=28.7625418060201, Blast_Score=86, Evalue=8e-18,
Organism=Saccharomyces cerevisiae, GI6324446, Length=250, Percent_Identity=28, Blast_Score=73, Evalue=6e-14,
Organism=Saccharomyces cerevisiae, GI6320125, Length=283, Percent_Identity=26.8551236749117, Blast_Score=72, Evalue=1e-13,
Organism=Drosophila melanogaster, GI17136226, Length=310, Percent_Identity=28.0645161290323, Blast_Score=124, Evalue=6e-29,
Organism=Drosophila melanogaster, GI24647881, Length=324, Percent_Identity=31.4814814814815, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI45550422, Length=312, Percent_Identity=26.2820512820513, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24663599, Length=323, Percent_Identity=26.9349845201238, Blast_Score=83, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24663595, Length=282, Percent_Identity=27.6595744680851, Blast_Score=80, Evalue=1e-15,

Paralogues:

None

Copy number: 2640 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2380 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase

Swissprot (AC and ID): MDH_NOVAD (Q2G946)

Other databases:

- EMBL:   CP000248
- RefSeq:   YP_496461.1
- ProteinModelPortal:   Q2G946
- SMR:   Q2G946
- STRING:   Q2G946
- GeneID:   3916479
- GenomeReviews:   CP000248_GR
- KEGG:   nar:Saro_1182
- NMPDR:   fig|48935.1.peg.866
- eggNOG:   COG0039
- HOGENOM:   HBG566126
- OMA:   ECTYVEG
- PhylomeDB:   Q2G946
- ProtClustDB:   PRK06223
- BioCyc:   NARO279238:SARO_1182-MONOMER
- GO:   GO:0005488
- GO:   GO:0006096
- HAMAP:   MF_00487
- InterPro:   IPR001557
- InterPro:   IPR022383
- InterPro:   IPR001236
- InterPro:   IPR015955
- InterPro:   IPR011275
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.90.110.10
- Gene3D:   G3DSA:3.40.50.720
- PIRSF:   PIRSF000102
- PRINTS:   PR00086
- TIGRFAMs:   TIGR01763

Pfam domain/function: PF02866 Ldh_1_C; PF00056 Ldh_1_N; SSF56327 Lactate_DH/Glyco_hydro_4_C

EC number: =1.1.1.37

Molecular weight: Translated: 33357; Mature: 33226

Theoretical pI: Translated: 4.50; Mature: 4.50

Prosite motif: NA

Important sites: ACT_SITE 176-176 BINDING 34-34 BINDING 83-83 BINDING 89-89 BINDING 96-96 BINDING 121-121 BINDING 152-152

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARKKIALIGAGNIGGTLAHLAAQKELGDIVLFDVVEGVPQGKALDLSQCGPVEGFDANI
CCCCEEEEEECCCCCHHHHHHHHHHHHCCEEEEEHHCCCCCCCEECHHCCCCCCCCCCEE
IGTNDYKGIAGADVIIVTAGVARKPGMSRDDLLGINLKVMKAVGEGIRDNAPDAFVICIT
EECCCCCCCCCCCEEEEECCCCCCCCCCCCCEEECHHHHHHHHCCCCCCCCCCEEEEEEC
NPLDAMVWALREFSGLPANKVVGMAGVLDSARFSTFLAWEFGVSIRDVNTFVLGGHGDTM
CCHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHEEEEEEECCEEEEEEEEEECCCCCEE
VPVTQYSTVNGIPVPDLVKMGLSTQEKIDAIVQRTRSGGGEIVGLLKTGSAFYAPAASGI
EEEEEECCCCCCCCHHHHHHCCCCHHHHHHHHHHHCCCCCEEEEEEECCCEEECCCCCCH
AMAEAYLNDQKRILPCAAYVDGEYGVNGLYVGVPVLIGANGVEKVIEIELDDEAKGNLQV
HHHHHHHCCCCEEEEEEEEECCCCCCCEEEEEEEEEECCCCCCEEEEEEECCCCCCCEEE
SVDAVKELLEACKGIDPSLA
EHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
ARKKIALIGAGNIGGTLAHLAAQKELGDIVLFDVVEGVPQGKALDLSQCGPVEGFDANI
CCCEEEEEECCCCCHHHHHHHHHHHHCCEEEEEHHCCCCCCCEECHHCCCCCCCCCCEE
IGTNDYKGIAGADVIIVTAGVARKPGMSRDDLLGINLKVMKAVGEGIRDNAPDAFVICIT
EECCCCCCCCCCCEEEEECCCCCCCCCCCCCEEECHHHHHHHHCCCCCCCCCCEEEEEEC
NPLDAMVWALREFSGLPANKVVGMAGVLDSARFSTFLAWEFGVSIRDVNTFVLGGHGDTM
CCHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHEEEEEEECCEEEEEEEEEECCCCCEE
VPVTQYSTVNGIPVPDLVKMGLSTQEKIDAIVQRTRSGGGEIVGLLKTGSAFYAPAASGI
EEEEEECCCCCCCCHHHHHHCCCCHHHHHHHHHHHCCCCCEEEEEEECCCEEECCCCCCH
AMAEAYLNDQKRILPCAAYVDGEYGVNGLYVGVPVLIGANGVEKVIEIELDDEAKGNLQV
HHHHHHHCCCCEEEEEEEEECCCCCCCEEEEEEEEEECCCCCCEEEEEEECCCCCCCEEE
SVDAVKELLEACKGIDPSLA
EHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA