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The map label for this gene is lpd3 [H]

Identifier: 87199200

GI number: 87199200

Start: 1217118

End: 1218518

Strand: Reverse

Name: lpd3 [H]

Synonym: Saro_1178

Alternate gene names: 87199200

Gene position: 1218518-1217118 (Counterclockwise)

Preceding gene: 87199201

Following gene: 87199199

Centisome position: 34.21

GC content: 64.6

Gene sequence:

>1401_bases
ATGGCTGAATACGATTACGACGTTCTTTTCATCGGTGCCGGTCCCGGCGGTTATGTCGGAGCCATCCGCACGGCGCAGCT
CGGACTGAAGACGGCTTGCGCCGAAGGGCGCGAGACGCTGGGCGGGACCTGCCTGAACGTCGGGTGCATTCCGTCGAAGG
CGCTGCTGCACGGTTCCGAGAAGTTCGACGAAGCCAGGAACGGCACTTTCGCCAGCTATGGCATCAAGACCGGCGCGGTC
GAACTCGACCTCGACGCGATGCAGGCGCAGAAGGCGGATTCGGTAAAGAGCCTGACGGGCGGCATCGAGTTCCTGTTCAA
GAAGAACAAGGTGACCTGGCTCAAGGGCTATGCCGCGTTCGAGGACGCGCACACGGTGACCGTCGCCGGCCAGAAGGTGA
CCGCGAAGAACATCGTCATCGCCACCGGTTCGAGCGTGACCCCGCTGCCGGGCGTGACCGTGGACAACGACGCGGGCGTG
ATCGTGGACAGCACTGGCGCACTGGCGCTGAACCGCGTGCCGCAGCACCTGGTGGTGATCGGCGGCGGCGTGATCGGGCT
TGAGCTGGGTTCGGTGTGGCGTCGCCTGGGCGCGAAGGTCACCGTGGTCGAATTCCTCGACCAGTTGCTGCCCGGCATGG
ACGGCGACGTTCGCAAGGAAGCGGCCAAGATCTTCAAGAAGCAGGGCATGGAGCTGAAGCTCGGCACCAAGGTGACGGGC
GTTGCGGTGAATGGCGGGACGGCGACGCTGACCGTCGAACCATCGAAGGGCGGCGAAGCGTCGACGATCGAGGCCGACTG
CGTGCTGGTCGCCATTGGCCGCCGTCCCAACGTGGACGGCCTCGGGCTCGACAAGATCGGACTTGAACTGAACGCACGCG
GGCAGATCGAGACCGACCACGACTTTGCCACGAAGATCCCCGGCGTATGGGCGATCGGCGACGTGATCCCCGGCCCGATG
CTGGCGCACAAGGCCGAGGACGAAGGCATCGCCGTGGCCGAGAACATCGCAGGGCTTACCGGCATCGTGAACCATGACGT
GATCCCGGGTGTGGTCTACACCATGCCGGAGTTCGCGGGCGTGGGGCTTACGGAAGAAGCGGCCAAGGAACGCGGCGAGA
TCAAGGTTGGCAAGTTTCCGATGCTGGCCAACAGCCGCGCAAAGACCAACCACGAGCCGGACGGTTTCGTGAAGGTCATT
TCCGACGCCAAGACCGACCGCGTGCTGGGCGTGTGGTGCATCGCGAGCGTTGCCGGCACGATGATCGCGCAGGCGGCGCA
AGCCATGGAATTCGGCGCGACAAGCGAAGACATCGCCTATACCTGCCATGCGCACCCGACCCATTCGGAAGCGCTGAAGG
AAGCCGCCATGGCGGTCACGGGCAAGCCGATCCACATGTGA

Upstream 100 bases:

>100_bases
CCTGCTGATCGATCTGTGATCCGAGCCACGCCTACTGACTTGAATCTAAGGCCGTTCGTGCTGAGCTTGGCGAAACACGA
ACGTGCAACGGAGCCGGAAA

Downstream 100 bases:

>100_bases
TCGCGCCCGGATTACGGCGATGAACGGGGCCGTCCTTCGGGGCGGCCCTTTTCGTTTGTGCCCTGCTGCCGACGGCGTTA
ACCGCAGGGTTTGAATCCAA

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase 3; LPD-3 [H]

Number of amino acids: Translated: 466; Mature: 465

Protein sequence:

>466_residues
MAEYDYDVLFIGAGPGGYVGAIRTAQLGLKTACAEGRETLGGTCLNVGCIPSKALLHGSEKFDEARNGTFASYGIKTGAV
ELDLDAMQAQKADSVKSLTGGIEFLFKKNKVTWLKGYAAFEDAHTVTVAGQKVTAKNIVIATGSSVTPLPGVTVDNDAGV
IVDSTGALALNRVPQHLVVIGGGVIGLELGSVWRRLGAKVTVVEFLDQLLPGMDGDVRKEAAKIFKKQGMELKLGTKVTG
VAVNGGTATLTVEPSKGGEASTIEADCVLVAIGRRPNVDGLGLDKIGLELNARGQIETDHDFATKIPGVWAIGDVIPGPM
LAHKAEDEGIAVAENIAGLTGIVNHDVIPGVVYTMPEFAGVGLTEEAAKERGEIKVGKFPMLANSRAKTNHEPDGFVKVI
SDAKTDRVLGVWCIASVAGTMIAQAAQAMEFGATSEDIAYTCHAHPTHSEALKEAAMAVTGKPIHM

Sequences:

>Translated_466_residues
MAEYDYDVLFIGAGPGGYVGAIRTAQLGLKTACAEGRETLGGTCLNVGCIPSKALLHGSEKFDEARNGTFASYGIKTGAV
ELDLDAMQAQKADSVKSLTGGIEFLFKKNKVTWLKGYAAFEDAHTVTVAGQKVTAKNIVIATGSSVTPLPGVTVDNDAGV
IVDSTGALALNRVPQHLVVIGGGVIGLELGSVWRRLGAKVTVVEFLDQLLPGMDGDVRKEAAKIFKKQGMELKLGTKVTG
VAVNGGTATLTVEPSKGGEASTIEADCVLVAIGRRPNVDGLGLDKIGLELNARGQIETDHDFATKIPGVWAIGDVIPGPM
LAHKAEDEGIAVAENIAGLTGIVNHDVIPGVVYTMPEFAGVGLTEEAAKERGEIKVGKFPMLANSRAKTNHEPDGFVKVI
SDAKTDRVLGVWCIASVAGTMIAQAAQAMEFGATSEDIAYTCHAHPTHSEALKEAAMAVTGKPIHM
>Mature_465_residues
AEYDYDVLFIGAGPGGYVGAIRTAQLGLKTACAEGRETLGGTCLNVGCIPSKALLHGSEKFDEARNGTFASYGIKTGAVE
LDLDAMQAQKADSVKSLTGGIEFLFKKNKVTWLKGYAAFEDAHTVTVAGQKVTAKNIVIATGSSVTPLPGVTVDNDAGVI
VDSTGALALNRVPQHLVVIGGGVIGLELGSVWRRLGAKVTVVEFLDQLLPGMDGDVRKEAAKIFKKQGMELKLGTKVTGV
AVNGGTATLTVEPSKGGEASTIEADCVLVAIGRRPNVDGLGLDKIGLELNARGQIETDHDFATKIPGVWAIGDVIPGPML
AHKAEDEGIAVAENIAGLTGIVNHDVIPGVVYTMPEFAGVGLTEEAAKERGEIKVGKFPMLANSRAKTNHEPDGFVKVIS
DAKTDRVLGVWCIASVAGTMIAQAAQAMEFGATSEDIAYTCHAHPTHSEALKEAAMAVTGKPIHM

Specific function: LPD-3 may substitute for lipoamide dehydrogenase of the 2-oxoglutarate dehydrogenase and pyruvate multienzyme complexes when the latter is inactive or missing [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=471, Percent_Identity=53.5031847133758, Blast_Score=471, Evalue=1e-133,
Organism=Homo sapiens, GI50301238, Length=476, Percent_Identity=28.5714285714286, Blast_Score=157, Evalue=1e-38,
Organism=Homo sapiens, GI33519430, Length=489, Percent_Identity=27.4028629856851, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI33519428, Length=489, Percent_Identity=27.4028629856851, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI33519426, Length=489, Percent_Identity=27.4028629856851, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI148277065, Length=489, Percent_Identity=27.4028629856851, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI148277071, Length=489, Percent_Identity=27.4028629856851, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI22035672, Length=476, Percent_Identity=29.4117647058824, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI291045266, Length=434, Percent_Identity=27.6497695852535, Blast_Score=117, Evalue=3e-26,
Organism=Homo sapiens, GI291045268, Length=427, Percent_Identity=25.0585480093677, Blast_Score=96, Evalue=7e-20,
Organism=Escherichia coli, GI1786307, Length=464, Percent_Identity=41.3793103448276, Blast_Score=309, Evalue=2e-85,
Organism=Escherichia coli, GI87082354, Length=466, Percent_Identity=30.9012875536481, Blast_Score=192, Evalue=6e-50,
Organism=Escherichia coli, GI1789915, Length=438, Percent_Identity=31.0502283105023, Blast_Score=177, Evalue=2e-45,
Organism=Escherichia coli, GI87081717, Length=468, Percent_Identity=29.0598290598291, Blast_Score=166, Evalue=4e-42,
Organism=Caenorhabditis elegans, GI32565766, Length=469, Percent_Identity=52.8784648187633, Blast_Score=461, Evalue=1e-130,
Organism=Caenorhabditis elegans, GI71983429, Length=454, Percent_Identity=29.5154185022026, Blast_Score=137, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI71983419, Length=454, Percent_Identity=29.5154185022026, Blast_Score=136, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI17557007, Length=476, Percent_Identity=27.7310924369748, Blast_Score=129, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI71982272, Length=489, Percent_Identity=25.7668711656442, Blast_Score=117, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17559934, Length=194, Percent_Identity=28.8659793814433, Blast_Score=70, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6321091, Length=475, Percent_Identity=51.1578947368421, Blast_Score=442, Evalue=1e-125,
Organism=Saccharomyces cerevisiae, GI6325240, Length=467, Percent_Identity=34.9036402569593, Blast_Score=253, Evalue=6e-68,
Organism=Saccharomyces cerevisiae, GI6325166, Length=466, Percent_Identity=26.6094420600858, Blast_Score=142, Evalue=1e-34,
Organism=Drosophila melanogaster, GI21358499, Length=471, Percent_Identity=52.8662420382166, Blast_Score=479, Evalue=1e-135,
Organism=Drosophila melanogaster, GI24640553, Length=480, Percent_Identity=28.5416666666667, Blast_Score=131, Evalue=1e-30,
Organism=Drosophila melanogaster, GI24640549, Length=480, Percent_Identity=28.5416666666667, Blast_Score=131, Evalue=1e-30,
Organism=Drosophila melanogaster, GI24640551, Length=484, Percent_Identity=28.099173553719, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI17737741, Length=488, Percent_Identity=26.6393442622951, Blast_Score=129, Evalue=6e-30,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 48553; Mature: 48422

Theoretical pI: Translated: 5.63; Mature: 5.63

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEYDYDVLFIGAGPGGYVGAIRTAQLGLKTACAEGRETLGGTCLNVGCIPSKALLHGSE
CCCCCEEEEEEECCCCCCCHHEHHHHHHHHHHHHCCHHHCCCEEEEECCCCCHHHHCCCH
KFDEARNGTFASYGIKTGAVELDLDAMQAQKADSVKSLTGGIEFLFKKNKVTWLKGYAAF
HHHHHCCCCEEECCCCCCEEEEEHHHHHHHHHHHHHHHHCCEEEEEECCCEEEEECEEEE
EDAHTVTVAGQKVTAKNIVIATGSSVTPLPGVTVDNDAGVIVDSTGALALNRVPQHLVVI
CCCCEEEEECCEEEECEEEEECCCCCCCCCCEEECCCCCEEEECCCCEEECCCCCEEEEE
GGGVIGLELGSVWRRLGAKVTVVEFLDQLLPGMDGDVRKEAAKIFKKQGMELKLGTKVTG
ECCEEEEHHHHHHHHHCCEEEHHHHHHHHCCCCCCHHHHHHHHHHHHCCCEEEECCEEEE
VAVNGGTATLTVEPSKGGEASTIEADCVLVAIGRRPNVDGLGLDKIGLELNARGQIETDH
EEEECCEEEEEEECCCCCCCCEEECCEEEEEECCCCCCCCCCCHHCCEEEECCCCCCCCC
DFATKIPGVWAIGDVIPGPMLAHKAEDEGIAVAENIAGLTGIVNHDVIPGVVYTMPEFAG
CHHHHCCCCEEECCCCCCCHHEECCCCCCCEEHHHHCHHHHHHCCCCCCCHHHCCCHHHC
VGLTEEAAKERGEIKVGKFPMLANSRAKTNHEPDGFVKVISDAKTDRVLGVWCIASVAGT
CCCCHHHHHHCCCEEECCCCEECCCCCCCCCCCCHHHEEECCCCCCCEEHHHHHHHHHHH
MIAQAAQAMEFGATSEDIAYTCHAHPTHSEALKEAAMAVTGKPIHM
HHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
AEYDYDVLFIGAGPGGYVGAIRTAQLGLKTACAEGRETLGGTCLNVGCIPSKALLHGSE
CCCCEEEEEEECCCCCCCHHEHHHHHHHHHHHHCCHHHCCCEEEEECCCCCHHHHCCCH
KFDEARNGTFASYGIKTGAVELDLDAMQAQKADSVKSLTGGIEFLFKKNKVTWLKGYAAF
HHHHHCCCCEEECCCCCCEEEEEHHHHHHHHHHHHHHHHCCEEEEEECCCEEEEECEEEE
EDAHTVTVAGQKVTAKNIVIATGSSVTPLPGVTVDNDAGVIVDSTGALALNRVPQHLVVI
CCCCEEEEECCEEEECEEEEECCCCCCCCCCEEECCCCCEEEECCCCEEECCCCCEEEEE
GGGVIGLELGSVWRRLGAKVTVVEFLDQLLPGMDGDVRKEAAKIFKKQGMELKLGTKVTG
ECCEEEEHHHHHHHHHCCEEEHHHHHHHHCCCCCCHHHHHHHHHHHHCCCEEEECCEEEE
VAVNGGTATLTVEPSKGGEASTIEADCVLVAIGRRPNVDGLGLDKIGLELNARGQIETDH
EEEECCEEEEEEECCCCCCCCEEECCEEEEEECCCCCCCCCCCHHCCEEEECCCCCCCCC
DFATKIPGVWAIGDVIPGPMLAHKAEDEGIAVAENIAGLTGIVNHDVIPGVVYTMPEFAG
CHHHHCCCCEEECCCCCCCHHEECCCCCCCEEHHHHCHHHHHHCCCCCCCHHHCCCHHHC
VGLTEEAAKERGEIKVGKFPMLANSRAKTNHEPDGFVKVISDAKTDRVLGVWCIASVAGT
CCCCHHHHHHCCCEEECCCCEECCCCCCCCCCCCHHHEEECCCCCCCEEHHHHHHHHHHH
MIAQAAQAMEFGATSEDIAYTCHAHPTHSEALKEAAMAVTGKPIHM
HHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]