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The map label for this gene is sucB [H]

Identifier: 87199201

GI number: 87199201

Start: 1218600

End: 1219826

Strand: Reverse

Name: sucB [H]

Synonym: Saro_1179

Alternate gene names: 87199201

Gene position: 1219826-1218600 (Counterclockwise)

Preceding gene: 87199202

Following gene: 87199200

Centisome position: 34.25

GC content: 66.99

Gene sequence:

>1227_bases
ATGTCGATTGAAGTGAAGGTTCCGACGCTGGGTGAAAGCGTCAGCGAAGCAACCGTCGGCCAATGGCTGAAGAAGCCCGG
CGAAGCCGTGGCGCTGGACGAGCCCATCGTCAGCCTGGAGACCGACAAGGTCGCGGTCGAAGTGCCCGCACCTGCCGCAG
GCGTGCTTGGCGCGCTGGTGGCGAACGAGGGCGACACCGTTGCGGTCGGCGCACTGCTTGCGCTGATCGAGGACGGCGTG
GCGGCGGCGGGCGCACAGGCCCCTGCCCCGCGCACCGAAGCGCCGGTTCCGCCCGCTTCGGCATCGGAAGCCCCTGCCGC
TCCGGCAGGCGACGCCGCCGTGCTTTCGCCCGCCGTGCGCCGCGCGGTGCTGGAATACGGTATCGACCCGTCGACCGTTA
AGGGCACCGGCAAGGACGGCCGCCTGACCAAGGAAGACGTCATGGCCGCCGCCGCCGCCAAGCAGGCCGCGCCCGCCGCT
GCGGTTTCGGCCCAGGCCGCAACCCCCGCGGCGGCTCCGGCCGGTGGTCGCAACGAAGAGCGCGTCAAGATGACGCGCCT
GCGCCAGACCATCGCCAAGCGCCTCAAGAGCGCGCAGGAAACCGCCGCCCTGCTCACCACGTTCAACGACGTGGACATGA
GCGCGGTGATGGAAGCGCGCGCCAAGTACAAGGACGTGTTCGAGAAGAAGCACGGCGTGAAGCTGGGCCTCATGTCGTTC
TTCGCCAAGGCTTCGGTCCTGGCGCTGAAGGACATCCCCTCGGTCAACGCGCAGATCCAGGGTGATGAGATCGTCTACTT
CGACTACGTGGACATCTCGGTCGCGGTCTCGGCCCCGAACGGGCTGGTCGTGCCGGTGGTGCGCGACGTCGACAAGATGA
GCTTCGCCGACATCGAGAAGTCGATCGCCGACTATGGCAAGAAGGCACGCGATGGCGCGCTGACCATGGCAGACATGGCG
GGCGGCACGTTCACCATCTCGAACGGCGGCGTTTTCGGGGGCCTGATGTCGACCCCGATCATCAACCCGCCGCAGTCGGC
CGTGCTTGGCCTGCACCGCATCGAGGATCGCCCGGTCGTGCGCAACGGCGAGATCGTGATCCGCCCGATGATGTACATCG
CGCTGTCCTACGACCATCGCATCATTGACGGACGCGAGGCGGTTACGGCACTCAAGACGATCAAGGAAGCGATCGAGGAT
CCGACGCGCCTGCTGATCGATCTGTGA

Upstream 100 bases:

>100_bases
TTGTCGCCGATGCGCTTGGCCTGTCGGTTCGCGGCGAGATCCGTCGCCAGAAGAAGCATTGAGCCGGGTAGGTTTTCCTT
CTCCCCAAGGAACGTGAGAT

Downstream 100 bases:

>100_bases
TCCGAGCCACGCCTACTGACTTGAATCTAAGGCCGTTCGTGCTGAGCTTGGCGAAACACGAACGTGCAACGGAGCCGGAA
AATGGCTGAATACGATTACG

Product: 2-oxoglutarate dehydrogenase E2 component

Products: NA

Alternate protein names: 2-oxoglutarate dehydrogenase complex component E2; OGDC-E2; Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 408; Mature: 407

Protein sequence:

>408_residues
MSIEVKVPTLGESVSEATVGQWLKKPGEAVALDEPIVSLETDKVAVEVPAPAAGVLGALVANEGDTVAVGALLALIEDGV
AAAGAQAPAPRTEAPVPPASASEAPAAPAGDAAVLSPAVRRAVLEYGIDPSTVKGTGKDGRLTKEDVMAAAAAKQAAPAA
AVSAQAATPAAAPAGGRNEERVKMTRLRQTIAKRLKSAQETAALLTTFNDVDMSAVMEARAKYKDVFEKKHGVKLGLMSF
FAKASVLALKDIPSVNAQIQGDEIVYFDYVDISVAVSAPNGLVVPVVRDVDKMSFADIEKSIADYGKKARDGALTMADMA
GGTFTISNGGVFGGLMSTPIINPPQSAVLGLHRIEDRPVVRNGEIVIRPMMYIALSYDHRIIDGREAVTALKTIKEAIED
PTRLLIDL

Sequences:

>Translated_408_residues
MSIEVKVPTLGESVSEATVGQWLKKPGEAVALDEPIVSLETDKVAVEVPAPAAGVLGALVANEGDTVAVGALLALIEDGV
AAAGAQAPAPRTEAPVPPASASEAPAAPAGDAAVLSPAVRRAVLEYGIDPSTVKGTGKDGRLTKEDVMAAAAAKQAAPAA
AVSAQAATPAAAPAGGRNEERVKMTRLRQTIAKRLKSAQETAALLTTFNDVDMSAVMEARAKYKDVFEKKHGVKLGLMSF
FAKASVLALKDIPSVNAQIQGDEIVYFDYVDISVAVSAPNGLVVPVVRDVDKMSFADIEKSIADYGKKARDGALTMADMA
GGTFTISNGGVFGGLMSTPIINPPQSAVLGLHRIEDRPVVRNGEIVIRPMMYIALSYDHRIIDGREAVTALKTIKEAIED
PTRLLIDL
>Mature_407_residues
SIEVKVPTLGESVSEATVGQWLKKPGEAVALDEPIVSLETDKVAVEVPAPAAGVLGALVANEGDTVAVGALLALIEDGVA
AAGAQAPAPRTEAPVPPASASEAPAAPAGDAAVLSPAVRRAVLEYGIDPSTVKGTGKDGRLTKEDVMAAAAAKQAAPAAA
VSAQAATPAAAPAGGRNEERVKMTRLRQTIAKRLKSAQETAALLTTFNDVDMSAVMEARAKYKDVFEKKHGVKLGLMSFF
AKASVLALKDIPSVNAQIQGDEIVYFDYVDISVAVSAPNGLVVPVVRDVDKMSFADIEKSIADYGKKARDGALTMADMAG
GTFTISNGGVFGGLMSTPIINPPQSAVLGLHRIEDRPVVRNGEIVIRPMMYIALSYDHRIIDGREAVTALKTIKEAIEDP
TRLLIDL

Specific function: The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of 3 enzymatic components:2-oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2) and l

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI19923748, Length=254, Percent_Identity=60.2362204724409, Blast_Score=315, Evalue=5e-86,
Organism=Homo sapiens, GI31711992, Length=433, Percent_Identity=31.1778290993072, Blast_Score=180, Evalue=3e-45,
Organism=Homo sapiens, GI203098753, Length=454, Percent_Identity=29.7356828193833, Blast_Score=179, Evalue=5e-45,
Organism=Homo sapiens, GI203098816, Length=454, Percent_Identity=29.9559471365639, Blast_Score=178, Evalue=1e-44,
Organism=Homo sapiens, GI110671329, Length=424, Percent_Identity=28.7735849056604, Blast_Score=159, Evalue=6e-39,
Organism=Homo sapiens, GI260898739, Length=160, Percent_Identity=35.625, Blast_Score=104, Evalue=1e-22,
Organism=Escherichia coli, GI1786946, Length=407, Percent_Identity=52.8255528255528, Blast_Score=419, Evalue=1e-118,
Organism=Escherichia coli, GI1786305, Length=425, Percent_Identity=33.4117647058824, Blast_Score=192, Evalue=5e-50,
Organism=Caenorhabditis elegans, GI25146366, Length=407, Percent_Identity=46.6830466830467, Blast_Score=334, Evalue=5e-92,
Organism=Caenorhabditis elegans, GI17560088, Length=439, Percent_Identity=29.6127562642369, Blast_Score=171, Evalue=5e-43,
Organism=Caenorhabditis elegans, GI17537937, Length=420, Percent_Identity=27.1428571428571, Blast_Score=161, Evalue=7e-40,
Organism=Caenorhabditis elegans, GI17538894, Length=313, Percent_Identity=29.7124600638978, Blast_Score=134, Evalue=1e-31,
Organism=Saccharomyces cerevisiae, GI6320352, Length=422, Percent_Identity=47.39336492891, Blast_Score=356, Evalue=4e-99,
Organism=Saccharomyces cerevisiae, GI6324258, Length=451, Percent_Identity=28.3813747228381, Blast_Score=132, Evalue=1e-31,
Organism=Drosophila melanogaster, GI24645909, Length=227, Percent_Identity=64.3171806167401, Blast_Score=303, Evalue=1e-82,
Organism=Drosophila melanogaster, GI18859875, Length=431, Percent_Identity=27.8422273781903, Blast_Score=150, Evalue=2e-36,
Organism=Drosophila melanogaster, GI20129315, Length=232, Percent_Identity=30.6034482758621, Blast_Score=114, Evalue=9e-26,
Organism=Drosophila melanogaster, GI24582497, Length=232, Percent_Identity=30.6034482758621, Blast_Score=114, Evalue=1e-25,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053
- InterPro:   IPR006255 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.61 [H]

Molecular weight: Translated: 42408; Mature: 42277

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIEVKVPTLGESVSEATVGQWLKKPGEAVALDEPIVSLETDKVAVEVPAPAAGVLGALV
CEEEEECCCCCCHHHHHHHHHHHHCCCCEEEECCCCEEEECCEEEEECCCCHHHHHHHHH
ANEGDTVAVGALLALIEDGVAAAGAQAPAPRTEAPVPPASASEAPAAPAGDAAVLSPAVR
CCCCCCHHHHHHHHHHHCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
RAVLEYGIDPSTVKGTGKDGRLTKEDVMAAAAAKQAAPAAAVSAQAATPAAAPAGGRNEE
HHHHHHCCCCCCEECCCCCCCCCHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCCCCHH
RVKMTRLRQTIAKRLKSAQETAALLTTFNDVDMSAVMEARAKYKDVFEKKHGVKLGLMSF
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
FAKASVLALKDIPSVNAQIQGDEIVYFDYVDISVAVSAPNGLVVPVVRDVDKMSFADIEK
HHHHHHHEECCCCCCCCEECCCEEEEEEEEEEEEEEECCCCEEEHHHHCCHHHHHHHHHH
SIADYGKKARDGALTMADMAGGTFTISNGGVFGGLMSTPIINPPQSAVLGLHRIEDRPVV
HHHHHCCCCCCCCEEEEECCCCEEEECCCCEECHHHCCCCCCCCHHHHHHHHHCCCCCEE
RNGEIVIRPMMYIALSYDHRIIDGREAVTALKTIKEAIEDPTRLLIDL
ECCCEEEEEEEEEEEECCCEEECCHHHHHHHHHHHHHHCCCHHHEECC
>Mature Secondary Structure 
SIEVKVPTLGESVSEATVGQWLKKPGEAVALDEPIVSLETDKVAVEVPAPAAGVLGALV
EEEEECCCCCCHHHHHHHHHHHHCCCCEEEECCCCEEEECCEEEEECCCCHHHHHHHHH
ANEGDTVAVGALLALIEDGVAAAGAQAPAPRTEAPVPPASASEAPAAPAGDAAVLSPAVR
CCCCCCHHHHHHHHHHHCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
RAVLEYGIDPSTVKGTGKDGRLTKEDVMAAAAAKQAAPAAAVSAQAATPAAAPAGGRNEE
HHHHHHCCCCCCEECCCCCCCCCHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCCCCHH
RVKMTRLRQTIAKRLKSAQETAALLTTFNDVDMSAVMEARAKYKDVFEKKHGVKLGLMSF
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
FAKASVLALKDIPSVNAQIQGDEIVYFDYVDISVAVSAPNGLVVPVVRDVDKMSFADIEK
HHHHHHHEECCCCCCCCEECCCEEEEEEEEEEEEEEECCCCEEEHHHHCCHHHHHHHHHH
SIADYGKKARDGALTMADMAGGTFTISNGGVFGGLMSTPIINPPQSAVLGLHRIEDRPVV
HHHHHCCCCCCCCEEEEECCCCEEEECCCCEECHHHCCCCCCCCHHHHHHHHHCCCCCEE
RNGEIVIRPMMYIALSYDHRIIDGREAVTALKTIKEAIEDPTRLLIDL
ECCCEEEEEEEEEEEECCCEEECCHHHHHHHHHHHHHHCCCHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12874367 [H]