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The map label for this gene is pspF [H]

Identifier: 87199176

GI number: 87199176

Start: 1202640

End: 1203701

Strand: Reverse

Name: pspF [H]

Synonym: Saro_1154

Alternate gene names: 87199176

Gene position: 1203701-1202640 (Counterclockwise)

Preceding gene: 87199190

Following gene: 87199175

Centisome position: 33.8

GC content: 68.55

Gene sequence:

>1062_bases
TTGCCGACCATTGGGAAAATGCGCTACTTCTTGCGCATGGATCGGGAAGTTCAGTTCATCGGCCAGTCCGGCGCATTCCT
CGACGCGGTCGAGCGCGCCAGCCGCGCCGCGCCGATGCGCCGACCGGTCCTGGTGATCGGCGAGCGCGGGACCGGCAAGG
AACTGATCGCCGAACGCCTCCACCGCCTGTCGACGCGCTGGCAGGAACCGCTGGTGACGATGAACTGCGCGGCCCTGCCC
GAAACCCTGATCGAAGCCGAACTGTTCGGGCACGAGGCGGGCGCCTTCACCGGAGCGACGCGCTCGCGCGTGGGGCGCTT
CGAGGAGGCCGACCGGGGCACGCTGTTCCTCGACGAGCTGGGCACGCTGTCGATGGGCGCGCAGGAGCGACTGCTGCGCG
CGGTCGAATATGGCGAGGTCACGCGGATCGGATCGAGCAAGCCGGTCCGCGTCGACGTGCGCATCGTGGCGGCAACGAAC
GAGGACCTGCCCAGGATGGCCGAGCAGGGCAGGTTCCGCGCGGACCTGCTCGACCGGCTGAGCTTCGAGGTCATTACCCT
GCCCCCGCTGCGCGTGCGCGAGGGGGACATCGCGGTGCTGGCCGACTACTTCGGGCGGCGCATGGCGGCGGAACTGCATT
GGGATGCCTGGCCGGGCTTCGCGCCGCAGACGCAGAGGGCGATGGAAGAATACCCCTGGCCCGGCAACGTGCGCGAGCTG
CGCAACGCGGTCGAGCGTGCAGTCTATCGCTGGGACGACTGGAGCACCCCCATCGCCCACGTGCAGTTCGACCCGTTCGA
GAGTCCGTGGAAGCCGACGGCATCGACGCGGGCGGACACGGCGGAAAGCGAAAGCGCGCCCACGCCGCGCAGCGCAGGCC
CGCATGACTTCGACGGCGTCAGCGACCTTCGCGCCGCAGTTGACGCACACGAGCGGGCGATCATCGAGCATCACCTGGGC
CGCAACCGCTACAACCAGCGGCAGACCGCCAAGGCGCTGGGACTGACCTACGACCAGCTTCGCCATTGCATGAAAAAGCA
CGGATTGATGGAGCGGGGTTGA

Upstream 100 bases:

>100_bases
GCCGGGATTGTTCATGGTAGAGCAAGCGGCGTGCCAAATCGCGGAGGCGGCGGATTTCCGGGCGTTTCCGCAGGTCATCC
AGCTTTAATGTTGGGGAATA

Downstream 100 bases:

>100_bases
GGGACGGTTGCTACCAGACGCAAGGCCTGAACGCGCTTCGTCCGCTTTGCGTCTTGCCCGGAAACCCACTAACGCCGCGA
CACGATGTCCCTCACCCGCC

Product: Fis family transcriptional regulator

Products: NA

Alternate protein names: Phage shock protein F [H]

Number of amino acids: Translated: 353; Mature: 352

Protein sequence:

>353_residues
MPTIGKMRYFLRMDREVQFIGQSGAFLDAVERASRAAPMRRPVLVIGERGTGKELIAERLHRLSTRWQEPLVTMNCAALP
ETLIEAELFGHEAGAFTGATRSRVGRFEEADRGTLFLDELGTLSMGAQERLLRAVEYGEVTRIGSSKPVRVDVRIVAATN
EDLPRMAEQGRFRADLLDRLSFEVITLPPLRVREGDIAVLADYFGRRMAAELHWDAWPGFAPQTQRAMEEYPWPGNVREL
RNAVERAVYRWDDWSTPIAHVQFDPFESPWKPTASTRADTAESESAPTPRSAGPHDFDGVSDLRAAVDAHERAIIEHHLG
RNRYNQRQTAKALGLTYDQLRHCMKKHGLMERG

Sequences:

>Translated_353_residues
MPTIGKMRYFLRMDREVQFIGQSGAFLDAVERASRAAPMRRPVLVIGERGTGKELIAERLHRLSTRWQEPLVTMNCAALP
ETLIEAELFGHEAGAFTGATRSRVGRFEEADRGTLFLDELGTLSMGAQERLLRAVEYGEVTRIGSSKPVRVDVRIVAATN
EDLPRMAEQGRFRADLLDRLSFEVITLPPLRVREGDIAVLADYFGRRMAAELHWDAWPGFAPQTQRAMEEYPWPGNVREL
RNAVERAVYRWDDWSTPIAHVQFDPFESPWKPTASTRADTAESESAPTPRSAGPHDFDGVSDLRAAVDAHERAIIEHHLG
RNRYNQRQTAKALGLTYDQLRHCMKKHGLMERG
>Mature_352_residues
PTIGKMRYFLRMDREVQFIGQSGAFLDAVERASRAAPMRRPVLVIGERGTGKELIAERLHRLSTRWQEPLVTMNCAALPE
TLIEAELFGHEAGAFTGATRSRVGRFEEADRGTLFLDELGTLSMGAQERLLRAVEYGEVTRIGSSKPVRVDVRIVAATNE
DLPRMAEQGRFRADLLDRLSFEVITLPPLRVREGDIAVLADYFGRRMAAELHWDAWPGFAPQTQRAMEEYPWPGNVRELR
NAVERAVYRWDDWSTPIAHVQFDPFESPWKPTASTRADTAESESAPTPRSAGPHDFDGVSDLRAAVDAHERAIIEHHLGR
NRYNQRQTAKALGLTYDQLRHCMKKHGLMERG

Specific function: Transcriptional activator for the phage shock protein (psp) operon (pspABCDE) and pspG gene [H]

COG id: COG1221

COG function: function code KT; Transcriptional regulators containing an AAA-type ATPase domain and a DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI87081872, Length=332, Percent_Identity=49.3975903614458, Blast_Score=334, Evalue=7e-93,
Organism=Escherichia coli, GI1790437, Length=230, Percent_Identity=51.7391304347826, Blast_Score=223, Evalue=2e-59,
Organism=Escherichia coli, GI1788550, Length=333, Percent_Identity=37.8378378378378, Blast_Score=213, Evalue=2e-56,
Organism=Escherichia coli, GI87082152, Length=343, Percent_Identity=38.7755102040816, Blast_Score=213, Evalue=2e-56,
Organism=Escherichia coli, GI1789233, Length=339, Percent_Identity=37.4631268436578, Blast_Score=212, Evalue=3e-56,
Organism=Escherichia coli, GI87082117, Length=232, Percent_Identity=48.2758620689655, Blast_Score=210, Evalue=9e-56,
Organism=Escherichia coli, GI1789087, Length=233, Percent_Identity=47.2103004291845, Blast_Score=208, Evalue=5e-55,
Organism=Escherichia coli, GI1790299, Length=226, Percent_Identity=47.787610619469, Blast_Score=200, Evalue=1e-52,
Organism=Escherichia coli, GI1788905, Length=230, Percent_Identity=40.8695652173913, Blast_Score=177, Evalue=1e-45,
Organism=Escherichia coli, GI1786524, Length=265, Percent_Identity=42.2641509433962, Blast_Score=177, Evalue=1e-45,
Organism=Escherichia coli, GI1787583, Length=335, Percent_Identity=31.6417910447761, Blast_Score=170, Evalue=1e-43,
Organism=Escherichia coli, GI1789828, Length=224, Percent_Identity=37.5, Blast_Score=122, Evalue=5e-29,
Organism=Escherichia coli, GI87081858, Length=223, Percent_Identity=31.390134529148, Blast_Score=110, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR014317 [H]

Pfam domain/function: PF02954 HTH_8; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 39946; Mature: 39815

Theoretical pI: Translated: 7.08; Mature: 7.08

Prosite motif: PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTIGKMRYFLRMDREVQFIGQSGAFLDAVERASRAAPMRRPVLVIGERGTGKELIAERL
CCCHHHHHHHHHHCCHHEEECCCCHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHH
HRLSTRWQEPLVTMNCAALPETLIEAELFGHEAGAFTGATRSRVGRFEEADRGTLFLDEL
HHHHHHHHCCCEEEEHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCCCCCEEEHHHH
GTLSMGAQERLLRAVEYGEVTRIGSSKPVRVDVRIVAATNEDLPRMAEQGRFRADLLDRL
CCCCCCHHHHHHHHHHHCCEEECCCCCCEEEEEEEEEECCCCHHHHHHCCCHHHHHHHHC
SFEVITLPPLRVREGDIAVLADYFGRRMAAELHWDAWPGFAPQTQRAMEEYPWPGNVREL
CCEEEECCCCEECCCCEEHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCCHHHH
RNAVERAVYRWDDWSTPIAHVQFDPFESPWKPTASTRADTAESESAPTPRSAGPHDFDGV
HHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCH
SDLRAAVDAHERAIIEHHLGRNRYNQRQTAKALGLTYDQLRHCMKKHGLMERG
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
PTIGKMRYFLRMDREVQFIGQSGAFLDAVERASRAAPMRRPVLVIGERGTGKELIAERL
CCHHHHHHHHHHCCHHEEECCCCHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHH
HRLSTRWQEPLVTMNCAALPETLIEAELFGHEAGAFTGATRSRVGRFEEADRGTLFLDEL
HHHHHHHHCCCEEEEHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCCCCCEEEHHHH
GTLSMGAQERLLRAVEYGEVTRIGSSKPVRVDVRIVAATNEDLPRMAEQGRFRADLLDRL
CCCCCCHHHHHHHHHHHCCEEECCCCCCEEEEEEEEEECCCCHHHHHHCCCHHHHHHHHC
SFEVITLPPLRVREGDIAVLADYFGRRMAAELHWDAWPGFAPQTQRAMEEYPWPGNVREL
CCEEEECCCCEECCCCEEHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCCHHHH
RNAVERAVYRWDDWSTPIAHVQFDPFESPWKPTASTRADTAESESAPTPRSAGPHDFDGV
HHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCH
SDLRAAVDAHERAIIEHHLGRNRYNQRQTAKALGLTYDQLRHCMKKHGLMERG
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8606168; 9097039; 9278503; 1712397; 7984428; 7920643 [H]