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The map label for this gene is recF

Identifier: 87199175

GI number: 87199175

Start: 1201482

End: 1202555

Strand: Reverse

Name: recF

Synonym: Saro_1153

Alternate gene names: 87199175

Gene position: 1202555-1201482 (Counterclockwise)

Preceding gene: 87199176

Following gene: 87199173

Centisome position: 33.76

GC content: 70.39

Gene sequence:

>1074_bases
ATGTCCCTCACCCGCCTGACCTTGCGCGACTTTCGCAACCATGCCGCGACGCGGCTGGAGGGCATGCGCGCGTTCAACGT
GCTGGTGGGCGAGAACGGCGCGGGCAAGACCAATGTGCTGGAGGCGATCAGCCTGCTGGCGCCGGGGCGCGGGCTACGGC
GCGCGCAGCCAGCAGAGATGGCCGGGCGCGAGGGGCCAGGCGGTTTTGCCATAGCAGCCGAAATGGAAGATGGGGCGGTG
CAGATCGGCACGGCGACATCTCCCGACGCGCCGGGCCGGCGGTCGGTGCGGATCAATGGCGTCGAAGGGCCCGCGGCACG
GCTGGCCGAATGGCTTTCGATAACCTGGCTGACCCCGGCGATGGACCGGATTTTCGCGGAGAGCGCGGGTTCGCGGCGGC
GCTTCCTTGACCGGCTGGTGCTGGCTCGCGACCCCGGACATGCGCGCAACGCGACCCGCTATGAAACGGCACTGCGCGAG
CGGAACCGCCTGCTGGGCGAGGTTGCCGAGCCTGACCCGCTATGGCTGGACGGGATAGAGGCGCAGATGGCCGAAACCGG
CGCAGCCATGGCGGCGGCGCGCACGGAACTGGTGGCGGACTTGTCGCGCGTGCTGGAAACCGTCCCCGATCAACCCTTTG
CCCGACCATCGCTGCGCTATGCCAGCGAAGTACCGCCGGACGCCGACGGCTTGCGGGCCATGCTGCGCGAAGGGCGCCGG
CGGGACCGGGCGGCAGGGCGTTCGCTGATCGGACCACATCGCGACGACCTGGCCGTCCTGCTCGCAGCGAAGAACGCCCC
CGCCGCAGACTGTTCGACGGGGGAGCAGAAGGCGATGCTGATCGCCATCGTGCTGGCCCACGCCGGACTGACGGCCGGGG
AGCGCCCGCGGCTGCTGCTGCTCGACGAGATTGCGGCGCACCTTGACCCGGTGCGGCGCGGCGCGCTGTTCGAACGGCTG
GCGACGAGCGGCGCGCAAGTGTGGATGACCGGGACCGAGATGGCGCCTTTCGCGCAGATCGCGGGGGAAAGCGCGATCTG
GTCTGTGCGCGACGGGGCCGTTTTCGCGCAGTAA

Upstream 100 bases:

>100_bases
GATGGAGCGGGGTTGAGGGACGGTTGCTACCAGACGCAAGGCCTGAACGCGCTTCGTCCGCTTTGCGTCTTGCCCGGAAA
CCCACTAACGCCGCGACACG

Downstream 100 bases:

>100_bases
CCGTCGCCGGTAAGCCGGGACTGCGCGATCAGGGCTTGATCGCCGCCTCCAGTGCATCGGCCACCTGTGCCTGCGTGGCA
GCCACGATCCTGTCCACGCC

Product: recombination protein F

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 357; Mature: 356

Protein sequence:

>357_residues
MSLTRLTLRDFRNHAATRLEGMRAFNVLVGENGAGKTNVLEAISLLAPGRGLRRAQPAEMAGREGPGGFAIAAEMEDGAV
QIGTATSPDAPGRRSVRINGVEGPAARLAEWLSITWLTPAMDRIFAESAGSRRRFLDRLVLARDPGHARNATRYETALRE
RNRLLGEVAEPDPLWLDGIEAQMAETGAAMAAARTELVADLSRVLETVPDQPFARPSLRYASEVPPDADGLRAMLREGRR
RDRAAGRSLIGPHRDDLAVLLAAKNAPAADCSTGEQKAMLIAIVLAHAGLTAGERPRLLLLDEIAAHLDPVRRGALFERL
ATSGAQVWMTGTEMAPFAQIAGESAIWSVRDGAVFAQ

Sequences:

>Translated_357_residues
MSLTRLTLRDFRNHAATRLEGMRAFNVLVGENGAGKTNVLEAISLLAPGRGLRRAQPAEMAGREGPGGFAIAAEMEDGAV
QIGTATSPDAPGRRSVRINGVEGPAARLAEWLSITWLTPAMDRIFAESAGSRRRFLDRLVLARDPGHARNATRYETALRE
RNRLLGEVAEPDPLWLDGIEAQMAETGAAMAAARTELVADLSRVLETVPDQPFARPSLRYASEVPPDADGLRAMLREGRR
RDRAAGRSLIGPHRDDLAVLLAAKNAPAADCSTGEQKAMLIAIVLAHAGLTAGERPRLLLLDEIAAHLDPVRRGALFERL
ATSGAQVWMTGTEMAPFAQIAGESAIWSVRDGAVFAQ
>Mature_356_residues
SLTRLTLRDFRNHAATRLEGMRAFNVLVGENGAGKTNVLEAISLLAPGRGLRRAQPAEMAGREGPGGFAIAAEMEDGAVQ
IGTATSPDAPGRRSVRINGVEGPAARLAEWLSITWLTPAMDRIFAESAGSRRRFLDRLVLARDPGHARNATRYETALRER
NRLLGEVAEPDPLWLDGIEAQMAETGAAMAAARTELVADLSRVLETVPDQPFARPSLRYASEVPPDADGLRAMLREGRRR
DRAAGRSLIGPHRDDLAVLLAAKNAPAADCSTGEQKAMLIAIVLAHAGLTAGERPRLLLLDEIAAHLDPVRRGALFERLA
TSGAQVWMTGTEMAPFAQIAGESAIWSVRDGAVFAQ

Specific function: The recF protein is involved in DNA metabolism; it is required for DNA replication and normal SOS inducibility. RecF binds preferentially to single-stranded, linear DNA. It also seems to bind ATP [H]

COG id: COG1195

COG function: function code L; Recombinational DNA repair ATPase (RecF pathway)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recF family [H]

Homologues:

Organism=Escherichia coli, GI1790135, Length=362, Percent_Identity=29.2817679558011, Blast_Score=122, Evalue=3e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001238
- InterPro:   IPR018078
- InterPro:   IPR003395 [H]

Pfam domain/function: PF02463 SMC_N [H]

EC number: NA

Molecular weight: Translated: 38341; Mature: 38210

Theoretical pI: Translated: 7.75; Mature: 7.75

Prosite motif: PS00617 RECF_1 ; PS00618 RECF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLTRLTLRDFRNHAATRLEGMRAFNVLVGENGAGKTNVLEAISLLAPGRGLRRAQPAEM
CCCHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCHHHHHHHHHHHCCCCCCCCCCCHHH
AGREGPGGFAIAAEMEDGAVQIGTATSPDAPGRRSVRINGVEGPAARLAEWLSITWLTPA
CCCCCCCCEEEEEECCCCEEEECCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHH
MDRIFAESAGSRRRFLDRLVLARDPGHARNATRYETALRERNRLLGEVAEPDPLWLDGIE
HHHHHHHCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCEECCHH
AQMAETGAAMAAARTELVADLSRVLETVPDQPFARPSLRYASEVPPDADGLRAMLREGRR
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHCCCCCHHHHHHHHHHHHH
RDRAAGRSLIGPHRDDLAVLLAAKNAPAADCSTGEQKAMLIAIVLAHAGLTAGERPRLLL
HHHHHCCCCCCCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCEEH
LDEIAAHLDPVRRGALFERLATSGAQVWMTGTEMAPFAQIAGESAIWSVRDGAVFAQ
HHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHCCCCCEEEECCCCEECC
>Mature Secondary Structure 
SLTRLTLRDFRNHAATRLEGMRAFNVLVGENGAGKTNVLEAISLLAPGRGLRRAQPAEM
CCHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCHHHHHHHHHHHCCCCCCCCCCCHHH
AGREGPGGFAIAAEMEDGAVQIGTATSPDAPGRRSVRINGVEGPAARLAEWLSITWLTPA
CCCCCCCCEEEEEECCCCEEEECCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHH
MDRIFAESAGSRRRFLDRLVLARDPGHARNATRYETALRERNRLLGEVAEPDPLWLDGIE
HHHHHHHCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCEECCHH
AQMAETGAAMAAARTELVADLSRVLETVPDQPFARPSLRYASEVPPDADGLRAMLREGRR
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHCCCCCHHHHHHHHHHHHH
RDRAAGRSLIGPHRDDLAVLLAAKNAPAADCSTGEQKAMLIAIVLAHAGLTAGERPRLLL
HHHHHCCCCCCCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCEEH
LDEIAAHLDPVRRGALFERLATSGAQVWMTGTEMAPFAQIAGESAIWSVRDGAVFAQ
HHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHCCCCCEEEECCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA