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The map label for this gene is cca [H]

Identifier: 87199043

GI number: 87199043

Start: 1059940

End: 1061142

Strand: Reverse

Name: cca [H]

Synonym: Saro_1021

Alternate gene names: 87199043

Gene position: 1061142-1059940 (Counterclockwise)

Preceding gene: 87199044

Following gene: 87199027

Centisome position: 29.79

GC content: 68.0

Gene sequence:

>1203_bases
ATGACTGACCGGTTGCCCAAGGCCGACTGGACCGCCCGGGACGACCTCGCGGCCCTCGTAGCTGCGCTCGATCCAGACTC
GCAGGGCAACTGCCGCTGGGTGGGCGGCGTCGTGCGAGACACGATCCTGGGCCTGCCCGCCAAGGACATCGACATGGCAA
CGACGTTGCCGCCGGACGAGACTGCCGCGCGACTTTCCACCGCAGGCATCAAATCCGTGCCGACCGGGATCGCCCACGGC
ACGGTGACCGCCGTGCTGCCGGGCGGACCGGTGGAGATCACCACGTTGCGCCGCGATGTCAGCACCGACGGACGCCATGC
CACGGTGAGCTTTTCGACCGACTGGCGCGATGACGCCGCGCGCCGCGATTTCACGATCAACGCGCTCTATGCCGACCCGC
GCACTCTGGAGGTGTTCGACTACCATGGCGGCCTGGCCGATCTTGCCGCGCGGCGCGTGCGCTTCATCGGCGATGCCCGC
CAGCGCATCCGCGAAGACTACCTGCGCATCCTTCGCTACTTCCGCTTCCAGGCACGGTTCGGGTCGATACCCGCCGATAC
CGAGGCGGAAAGCGCCGTTTCGGAACTGGCTGCGGGCCTGAAGGGGCTTTCCCGCGAACGCGTGGGCTGGGAGCTGATGA
ACCTTCTCGGCCTGCCCGACCCGGCACCAACCATGCGCCGCATGGCCGAACTGGGCGTTTTGGCGCAGGTGCTGCCGGAG
ACTGCCGCAGATGGCCTTGATGCGCTTGAGGCGCTGATCGGCAATGAGCAGTCCGCCAGAGCAGATGCGGTGGCGATCCG
GCGGCTAGCAGCGCTGCTTCCCGCCGACCGGCATTTGGCGGAACTGGTCGCGGCGCGATTGAGGCTTTCGGCCGCACAGC
GCAAGCGCCTCGCAACGGTCGCGGCGCCTTCCGGTGCGGACGGCGATGCGCGGGCCCTTGCCTACCGCATCGGGATCGAG
GAAGCACGCGACCGACTGCTGATTGCCGGAAGGCCCGTTGCCGCACTTGATGGATGGCAGGCCCCCGCCTTGCCACTGAA
GGGCGGAGAGATCGTGGCCCGCGGGGTCACGGCCGGGCCGGAGGTTGCGCGAACGCTGCGTGCGGTCGAAGACCAGTGGA
TCAGCGAAGGCTTCCCTGACGGCGCAAGAGTTTCCGCAATCCTCGATCAAGTCCTGGGACATACCAGCGCACCACGCGAC
TGA

Upstream 100 bases:

>100_bases
CACTACATCGAGATCATGTGGAACGAGCATCGCATCTGGGGCGTCACCGCCGCGATCATCTCCAACCTTTCGAAGCGCAT
GGCCTGGAAGGACGCGGCCG

Downstream 100 bases:

>100_bases
TCGCGCCAAGACCCGTGCGCAGGCCAGGCATTGGCTTGAAGCGCTTCAGTCTTTTGCGCTTTTCCGGGGGCAGCGACCAG
ACGAGCTCGGGCTGGATCAC

Product: polynucleotide adenylyltransferase region

Products: NA

Alternate protein names: tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl- transferase; tRNA nucleotidyltransferase; tRNA-NT [H]

Number of amino acids: Translated: 400; Mature: 399

Protein sequence:

>400_residues
MTDRLPKADWTARDDLAALVAALDPDSQGNCRWVGGVVRDTILGLPAKDIDMATTLPPDETAARLSTAGIKSVPTGIAHG
TVTAVLPGGPVEITTLRRDVSTDGRHATVSFSTDWRDDAARRDFTINALYADPRTLEVFDYHGGLADLAARRVRFIGDAR
QRIREDYLRILRYFRFQARFGSIPADTEAESAVSELAAGLKGLSRERVGWELMNLLGLPDPAPTMRRMAELGVLAQVLPE
TAADGLDALEALIGNEQSARADAVAIRRLAALLPADRHLAELVAARLRLSAAQRKRLATVAAPSGADGDARALAYRIGIE
EARDRLLIAGRPVAALDGWQAPALPLKGGEIVARGVTAGPEVARTLRAVEDQWISEGFPDGARVSAILDQVLGHTSAPRD

Sequences:

>Translated_400_residues
MTDRLPKADWTARDDLAALVAALDPDSQGNCRWVGGVVRDTILGLPAKDIDMATTLPPDETAARLSTAGIKSVPTGIAHG
TVTAVLPGGPVEITTLRRDVSTDGRHATVSFSTDWRDDAARRDFTINALYADPRTLEVFDYHGGLADLAARRVRFIGDAR
QRIREDYLRILRYFRFQARFGSIPADTEAESAVSELAAGLKGLSRERVGWELMNLLGLPDPAPTMRRMAELGVLAQVLPE
TAADGLDALEALIGNEQSARADAVAIRRLAALLPADRHLAELVAARLRLSAAQRKRLATVAAPSGADGDARALAYRIGIE
EARDRLLIAGRPVAALDGWQAPALPLKGGEIVARGVTAGPEVARTLRAVEDQWISEGFPDGARVSAILDQVLGHTSAPRD
>Mature_399_residues
TDRLPKADWTARDDLAALVAALDPDSQGNCRWVGGVVRDTILGLPAKDIDMATTLPPDETAARLSTAGIKSVPTGIAHGT
VTAVLPGGPVEITTLRRDVSTDGRHATVSFSTDWRDDAARRDFTINALYADPRTLEVFDYHGGLADLAARRVRFIGDARQ
RIREDYLRILRYFRFQARFGSIPADTEAESAVSELAAGLKGLSRERVGWELMNLLGLPDPAPTMRRMAELGVLAQVLPET
AADGLDALEALIGNEQSARADAVAIRRLAALLPADRHLAELVAARLRLSAAQRKRLATVAAPSGADGDARALAYRIGIEE
ARDRLLIAGRPVAALDGWQAPALPLKGGEIVARGVTAGPEVARTLRAVEDQWISEGFPDGARVSAILDQVLGHTSAPRD

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 3 subfamily [H]

Homologues:

Organism=Homo sapiens, GI155030240, Length=368, Percent_Identity=34.7826086956522, Blast_Score=188, Evalue=9e-48,
Organism=Escherichia coli, GI87081691, Length=245, Percent_Identity=29.3877551020408, Blast_Score=81, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI71995920, Length=379, Percent_Identity=32.1899736147757, Blast_Score=166, Evalue=2e-41,
Organism=Drosophila melanogaster, GI281360112, Length=360, Percent_Identity=33.6111111111111, Blast_Score=161, Evalue=6e-40,
Organism=Drosophila melanogaster, GI21357337, Length=360, Percent_Identity=33.6111111111111, Blast_Score=161, Evalue=6e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023068
- InterPro:   IPR002646 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.21; =2.7.7.25 [H]

Molecular weight: Translated: 42856; Mature: 42725

Theoretical pI: Translated: 5.80; Mature: 5.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDRLPKADWTARDDLAALVAALDPDSQGNCRWVGGVVRDTILGLPAKDIDMATTLPPDE
CCCCCCCCCCCCHHHHHHHHHHCCCCCCCCEEEECHHHHHHHHCCCCCCCCCCCCCCCCH
TAARLSTAGIKSVPTGIAHGTVTAVLPGGPVEITTLRRDVSTDGRHATVSFSTDWRDDAA
HHHHHHHCCHHHCCCCCCCCCEEEEECCCCEEEEEEHHCCCCCCCEEEEEECCCCCCHHH
RRDFTINALYADPRTLEVFDYHGGLADLAARRVRFIGDARQRIREDYLRILRYFRFQARF
CCCEEEEEEECCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GSIPADTEAESAVSELAAGLKGLSRERVGWELMNLLGLPDPAPTMRRMAELGVLAQVLPE
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
TAADGLDALEALIGNEQSARADAVAIRRLAALLPADRHLAELVAARLRLSAAQRKRLATV
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHE
AAPSGADGDARALAYRIGIEEARDRLLIAGRPVAALDGWQAPALPLKGGEIVARGVTAGP
ECCCCCCCCHHHHHHHHCHHHHCCCEEECCCCCHHCCCCCCCCCCCCCCCEEEECCCCCH
EVARTLRAVEDQWISEGFPDGARVSAILDQVLGHTSAPRD
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
TDRLPKADWTARDDLAALVAALDPDSQGNCRWVGGVVRDTILGLPAKDIDMATTLPPDE
CCCCCCCCCCCHHHHHHHHHHCCCCCCCCEEEECHHHHHHHHCCCCCCCCCCCCCCCCH
TAARLSTAGIKSVPTGIAHGTVTAVLPGGPVEITTLRRDVSTDGRHATVSFSTDWRDDAA
HHHHHHHCCHHHCCCCCCCCCEEEEECCCCEEEEEEHHCCCCCCCEEEEEECCCCCCHHH
RRDFTINALYADPRTLEVFDYHGGLADLAARRVRFIGDARQRIREDYLRILRYFRFQARF
CCCEEEEEEECCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GSIPADTEAESAVSELAAGLKGLSRERVGWELMNLLGLPDPAPTMRRMAELGVLAQVLPE
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
TAADGLDALEALIGNEQSARADAVAIRRLAALLPADRHLAELVAARLRLSAAQRKRLATV
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHE
AAPSGADGDARALAYRIGIEEARDRLLIAGRPVAALDGWQAPALPLKGGEIVARGVTAGP
ECCCCCCCCHHHHHHHHCHHHHCCCEEECCCCCHHCCCCCCCCCCCCCCCEEEECCCCCH
EVARTLRAVEDQWISEGFPDGARVSAILDQVLGHTSAPRD
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA