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The map label for this gene is rimO

Identifier: 87198868

GI number: 87198868

Start: 897013

End: 898428

Strand: Reverse

Name: rimO

Synonym: Saro_0846

Alternate gene names: 87198868

Gene position: 898428-897013 (Counterclockwise)

Preceding gene: 87198870

Following gene: 87198862

Centisome position: 25.23

GC content: 65.82

Gene sequence:

>1416_bases
ATGGGCCGCGCCAAGATGGCAAGCCAGATTCCCCCGCGAGAGATTCCCGAAGCCCCCAAGGTCGGCATGGTCAGCCTTGG
CTGTCCCAAGGCGCTCGTCGATTCCGAACGCATCCTGACCCGCCTCCGGGCCGACGGCTATGCCATGAGCGCGGACTATG
CCGGGGCCGACGTCGTGCTCGTCAACACCTGCGGCTTCCTCGATTCCGCCAAGGAAGAAAGCCTCTCCGCCATTGGCGAG
GCGATTGCCGAGAACGGCCGCGTGATCGTGACGGGCTGCATGGGCAACGAGGCGGAAGCTATCCGGGCCAAGTTCCCGCA
GGTCCTCGCCGTGACCGGCGCTCACCAGTACGAAGCGGTGGTCGAGGCCGTGCACGAAGCCGCCCCGCCCAGCATGGGGC
CCTATATCGACCTCATTCCGCAGGCGGACCCGGGCGACGTCAAGCTCACGCCGCGGCACTACAGCTACCTGAAGATCTCG
GAAGGCTGCAACCACGCCTGTGCGTTCTGCATCATCCCGTCACTGCGCGGCAAGCTCGCCAGTCGCCGGATCGACGCGGT
CCTGCGCGAGGCGGAAAAGCTCGTCGCGGCAGGCACGAAGGAACTGCTGGTCATCAGCCAGGACACTTCTGCCTATGGCG
TCGACGTCCGCCACGAGGAACGCATGTGGAAGGGCCGCCCCGTCCGCACCCACATGACCGATCTGGCCAGGGAGCTCGGC
CAGCTCCGCACGCCGCAGGGCGAAACCCCGTGGGTCCGCCTGCACTATGTTTATCCCTATCCGCACGTCGACGCGGTCAT
TCCGCTCATGGCGGAAGGGCTGGTGACGCCGTACCTCGACATTCCGTTCCAGCACGCCAGCCCGAACGTGCTGCGCGCGA
TGAAGCGACCGGCGAACGAAGCCAAGGTCCTCGAGCGGATCAAGGCCTGGCGCGAAATCTGCCCGGACCTCGCCATCCGC
TCCAGCTTCGTCGTGGGTTTCCCCGGAGAAACGCAGGCCGATTTCGAGTATCTGCTTGAATGGCTTGAAGAAGCGCAGCT
CGACAGGGTCGGCGGATTCCGCTTCGAGCCGGTGGCTGGCGCGGCGGCCAACGATCTGCCCGACCAGGTGCCGGAAGAAA
TCAAGGAAGAACGCTACGCCCGGCTCATGGAAAAGACGGAGGCGATCAGCGCTGCCCGGCTTTCCGCAAAGGTCGGCCGC
CATATCAAGGTGATAGTCGACGAGGTCGGCGAGCCTGACGAGGATGGCGACATCGGCGCCACCGCGCGGTCCCAGGCCGA
TGCGCCCGAAATCGACGGCGCGGTCTATCTGCGCAACGTGCCCGCAAGCCTGAAGCCGGGCGACTTTGCCGAAGTGCTGG
TCGAGGACGCCGATGCCCACGACCTCTACGGGGTCATCGCGGACCGCCAGGGATAA

Upstream 100 bases:

>100_bases
TCCTTCTTTTTCCGCCCCTTCCGGCTCCAGTTGCTAGACCCGCGCACCCGTCAATGCAATCACCCGACCGCTACAGGTGA
CGCGCCGTGCATTTTCGCCT

Downstream 100 bases:

>100_bases
GGGTCATGCTGTGTCCTTGCAGGCGGCGGCCAGCAAGGTCTCGCGCAGCCGGGATACCTCGGGAAGATCGAGGCCCGACA
AGTCCGCGATCCCTTCGATC

Product: ribosomal protein S12 methylthiotransferase

Products: NA

Alternate protein names: S12 MTTase; S12 methylthiotransferase; Ribosome maturation factor RimO

Number of amino acids: Translated: 471; Mature: 470

Protein sequence:

>471_residues
MGRAKMASQIPPREIPEAPKVGMVSLGCPKALVDSERILTRLRADGYAMSADYAGADVVLVNTCGFLDSAKEESLSAIGE
AIAENGRVIVTGCMGNEAEAIRAKFPQVLAVTGAHQYEAVVEAVHEAAPPSMGPYIDLIPQADPGDVKLTPRHYSYLKIS
EGCNHACAFCIIPSLRGKLASRRIDAVLREAEKLVAAGTKELLVISQDTSAYGVDVRHEERMWKGRPVRTHMTDLARELG
QLRTPQGETPWVRLHYVYPYPHVDAVIPLMAEGLVTPYLDIPFQHASPNVLRAMKRPANEAKVLERIKAWREICPDLAIR
SSFVVGFPGETQADFEYLLEWLEEAQLDRVGGFRFEPVAGAAANDLPDQVPEEIKEERYARLMEKTEAISAARLSAKVGR
HIKVIVDEVGEPDEDGDIGATARSQADAPEIDGAVYLRNVPASLKPGDFAEVLVEDADAHDLYGVIADRQG

Sequences:

>Translated_471_residues
MGRAKMASQIPPREIPEAPKVGMVSLGCPKALVDSERILTRLRADGYAMSADYAGADVVLVNTCGFLDSAKEESLSAIGE
AIAENGRVIVTGCMGNEAEAIRAKFPQVLAVTGAHQYEAVVEAVHEAAPPSMGPYIDLIPQADPGDVKLTPRHYSYLKIS
EGCNHACAFCIIPSLRGKLASRRIDAVLREAEKLVAAGTKELLVISQDTSAYGVDVRHEERMWKGRPVRTHMTDLARELG
QLRTPQGETPWVRLHYVYPYPHVDAVIPLMAEGLVTPYLDIPFQHASPNVLRAMKRPANEAKVLERIKAWREICPDLAIR
SSFVVGFPGETQADFEYLLEWLEEAQLDRVGGFRFEPVAGAAANDLPDQVPEEIKEERYARLMEKTEAISAARLSAKVGR
HIKVIVDEVGEPDEDGDIGATARSQADAPEIDGAVYLRNVPASLKPGDFAEVLVEDADAHDLYGVIADRQG
>Mature_470_residues
GRAKMASQIPPREIPEAPKVGMVSLGCPKALVDSERILTRLRADGYAMSADYAGADVVLVNTCGFLDSAKEESLSAIGEA
IAENGRVIVTGCMGNEAEAIRAKFPQVLAVTGAHQYEAVVEAVHEAAPPSMGPYIDLIPQADPGDVKLTPRHYSYLKISE
GCNHACAFCIIPSLRGKLASRRIDAVLREAEKLVAAGTKELLVISQDTSAYGVDVRHEERMWKGRPVRTHMTDLARELGQ
LRTPQGETPWVRLHYVYPYPHVDAVIPLMAEGLVTPYLDIPFQHASPNVLRAMKRPANEAKVLERIKAWREICPDLAIRS
SFVVGFPGETQADFEYLLEWLEEAQLDRVGGFRFEPVAGAAANDLPDQVPEEIKEERYARLMEKTEAISAARLSAKVGRH
IKVIVDEVGEPDEDGDIGATARSQADAPEIDGAVYLRNVPASLKPGDFAEVLVEDADAHDLYGVIADRQG

Specific function: Catalyzes the methylthiolation of an aspartic acid residue of ribosomal protein S12

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI93277076, Length=414, Percent_Identity=26.8115942028986, Blast_Score=140, Evalue=2e-33,
Organism=Homo sapiens, GI28872782, Length=491, Percent_Identity=26.0692464358452, Blast_Score=119, Evalue=6e-27,
Organism=Homo sapiens, GI28872784, Length=493, Percent_Identity=25.9634888438134, Blast_Score=119, Evalue=6e-27,
Organism=Escherichia coli, GI1787057, Length=447, Percent_Identity=61.5212527964206, Blast_Score=549, Evalue=1e-157,
Organism=Escherichia coli, GI1786882, Length=400, Percent_Identity=26.75, Blast_Score=137, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI17553146, Length=472, Percent_Identity=23.728813559322, Blast_Score=112, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI71996771, Length=286, Percent_Identity=25.5244755244755, Blast_Score=72, Evalue=4e-13,
Organism=Drosophila melanogaster, GI21356207, Length=493, Percent_Identity=23.7322515212982, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI19922432, Length=412, Percent_Identity=26.2135922330097, Blast_Score=115, Evalue=6e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RIMO_NOVAD (Q2GA32)

Other databases:

- EMBL:   CP000248
- RefSeq:   YP_496125.1
- ProteinModelPortal:   Q2GA32
- SMR:   Q2GA32
- STRING:   Q2GA32
- GeneID:   3915901
- GenomeReviews:   CP000248_GR
- KEGG:   nar:Saro_0846
- NMPDR:   fig|48935.1.peg.3437
- eggNOG:   COG0621
- HOGENOM:   HBG457663
- OMA:   KADAPEI
- PhylomeDB:   Q2GA32
- ProtClustDB:   PRK14862
- BioCyc:   NARO279238:SARO_0846-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01865
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR012340
- InterPro:   IPR007197
- InterPro:   IPR005840
- InterPro:   IPR002792
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR11918
- SMART:   SM00729
- TIGRFAMs:   TIGR01125
- TIGRFAMs:   TIGR00089

Pfam domain/function: PF04055 Radical_SAM; PF00919 UPF0004

EC number: NA

Molecular weight: Translated: 51435; Mature: 51304

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: PS51449 MTTASE_N; PS01278 MTTASE_RADICAL; PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGRAKMASQIPPREIPEAPKVGMVSLGCPKALVDSERILTRLRADGYAMSADYAGADVVL
CCCHHHHHCCCCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHCCCEEEECCCCCCCEEE
VNTCGFLDSAKEESLSAIGEAIAENGRVIVTGCMGNEAEAIRAKFPQVLAVTGAHQYEAV
EECCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHCCCEEEECCCHHHHHH
VEAVHEAAPPSMGPYIDLIPQADPGDVKLTPRHYSYLKISEGCNHACAFCIIPSLRGKLA
HHHHHHHCCCCCCCCEEECCCCCCCCEEECCCCCEEEEECCCCCHHHHHEEEHHHHHHHH
SRRIDAVLREAEKLVAAGTKELLVISQDTSAYGVDVRHEERMWKGRPVRTHMTDLARELG
HHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHH
QLRTPQGETPWVRLHYVYPYPHVDAVIPLMAEGLVTPYLDIPFQHASPNVLRAMKRPANE
HCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHCCCCCHHCCCCCCCCCHHHHHHHCCCHH
AKVLERIKAWREICPDLAIRSSFVVGFPGETQADFEYLLEWLEEAQLDRVGGFRFEPVAG
HHHHHHHHHHHHHCHHHHHHCCEEEECCCCCCHHHHHHHHHHHHHHHHHHCCEEECCCCC
AAANDLPDQVPEEIKEERYARLMEKTEAISAARLSAKVGRHIKVIVDEVGEPDEDGDIGA
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHCCCCCCCCCCCC
TARSQADAPEIDGAVYLRNVPASLKPGDFAEVLVEDADAHDLYGVIADRQG
CCCCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHCCCCCHHHEEEECCCCC
>Mature Secondary Structure 
GRAKMASQIPPREIPEAPKVGMVSLGCPKALVDSERILTRLRADGYAMSADYAGADVVL
CCHHHHHCCCCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHCCCEEEECCCCCCCEEE
VNTCGFLDSAKEESLSAIGEAIAENGRVIVTGCMGNEAEAIRAKFPQVLAVTGAHQYEAV
EECCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHCCCEEEECCCHHHHHH
VEAVHEAAPPSMGPYIDLIPQADPGDVKLTPRHYSYLKISEGCNHACAFCIIPSLRGKLA
HHHHHHHCCCCCCCCEEECCCCCCCCEEECCCCCEEEEECCCCCHHHHHEEEHHHHHHHH
SRRIDAVLREAEKLVAAGTKELLVISQDTSAYGVDVRHEERMWKGRPVRTHMTDLARELG
HHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHH
QLRTPQGETPWVRLHYVYPYPHVDAVIPLMAEGLVTPYLDIPFQHASPNVLRAMKRPANE
HCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHCCCCCHHCCCCCCCCCHHHHHHHCCCHH
AKVLERIKAWREICPDLAIRSSFVVGFPGETQADFEYLLEWLEEAQLDRVGGFRFEPVAG
HHHHHHHHHHHHHCHHHHHHCCEEEECCCCCCHHHHHHHHHHHHHHHHHHCCEEECCCCC
AAANDLPDQVPEEIKEERYARLMEKTEAISAARLSAKVGRHIKVIVDEVGEPDEDGDIGA
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHCCCCCCCCCCCC
TARSQADAPEIDGAVYLRNVPASLKPGDFAEVLVEDADAHDLYGVIADRQG
CCCCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHCCCCCHHHEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA