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The map label for this gene is xthA [H]

Identifier: 87198182

GI number: 87198182

Start: 154525

End: 155304

Strand: Direct

Name: xthA [H]

Synonym: Saro_0156

Alternate gene names: 87198182

Gene position: 154525-155304 (Clockwise)

Preceding gene: 87198181

Following gene: 87198183

Centisome position: 4.34

GC content: 62.69

Gene sequence:

>780_bases
ATGACGCTCAAGGTCGCAAGTTTCAACATCAACGGGATCAAGGCCCGTCTGCCACGCCTGCTCGAATGGTTGGAAGAGAC
GCGGCCGTCGGTCGCATGCTTGCAGGAAATCAAGACTCAGGACGAAGGCTTTCCGGCCGAGGAGTTCGAGAAGATCGGCT
ATCGCGCGATCTGGCATGGGCAGAAGGGCTTCAACGGCGTGGCGATCCTCGCCGATGGGGAGCAGCCCGTCGAGGTGCAG
CGCGGCCTGGCTGGCGATCCCGAGGACGAGCATTCGCGCTATCTGGAAGCCGACGTGTTCGGTCTTCGGGTGGTGTGCAT
CTACCTGCCGAATGGTAATCCGCAGCCGGGCCCCAAGTTCGACTACAAGCTTCGCTGGATGGAACGCCTCCGCGCCCGCA
TGGCCGATATTGCGTCCGAGGAAGTGCCGGCGCTTGTCATTGGCGACTACAACGTGATCCCCGAGGACAAGGATACCTTT
TCGGTCAAGGCAATGGCGAGCGATGCATTGATGCAGCCCGAATCGCGCGATGCCTATCGCCGGCTGCTCAACGACGGGTG
GACCGATGCCATCGACCTGTTCAATCCGCAGGGCGGCGTGTGGACGTTCTGGGACTACCAAGCCGGTGCATGGCAGCGCG
ACCACGGCTTCCGGATCGACCATGCCCTGCTATCGCCGGAGCTTGCCGACAGGCTGGTCGCAGCGGGCGTCGACAAGGAT
TACCGCGGCCGCGAGAAGGCCAGTGATCATGCGCCGGTCTGGGTCACCTTGCGGGACTGA

Upstream 100 bases:

>100_bases
GCGGCATCGGGTTGCGGCTGCGGGGCTTCCTTCGCCATTTGACGCCCGGTCGGGCGTCGTTTCGCAGCGCCCATCCGGCA
CCAGCAGGAATGCCGAAAAG

Downstream 100 bases:

>100_bases
AACGGTTTATCGTACGTAGACAACGCTTTGCGGATTCCCAATCGGCATGAACGGCGGCCTGTGCATGGCGTCACTGCGCG
TTAAGCCGGGGCAGGGCATC

Product: exodeoxyribonuclease III

Products: NA

Alternate protein names: EXO III; Exonuclease III; AP endonuclease VI [H]

Number of amino acids: Translated: 259; Mature: 258

Protein sequence:

>259_residues
MTLKVASFNINGIKARLPRLLEWLEETRPSVACLQEIKTQDEGFPAEEFEKIGYRAIWHGQKGFNGVAILADGEQPVEVQ
RGLAGDPEDEHSRYLEADVFGLRVVCIYLPNGNPQPGPKFDYKLRWMERLRARMADIASEEVPALVIGDYNVIPEDKDTF
SVKAMASDALMQPESRDAYRRLLNDGWTDAIDLFNPQGGVWTFWDYQAGAWQRDHGFRIDHALLSPELADRLVAAGVDKD
YRGREKASDHAPVWVTLRD

Sequences:

>Translated_259_residues
MTLKVASFNINGIKARLPRLLEWLEETRPSVACLQEIKTQDEGFPAEEFEKIGYRAIWHGQKGFNGVAILADGEQPVEVQ
RGLAGDPEDEHSRYLEADVFGLRVVCIYLPNGNPQPGPKFDYKLRWMERLRARMADIASEEVPALVIGDYNVIPEDKDTF
SVKAMASDALMQPESRDAYRRLLNDGWTDAIDLFNPQGGVWTFWDYQAGAWQRDHGFRIDHALLSPELADRLVAAGVDKD
YRGREKASDHAPVWVTLRD
>Mature_258_residues
TLKVASFNINGIKARLPRLLEWLEETRPSVACLQEIKTQDEGFPAEEFEKIGYRAIWHGQKGFNGVAILADGEQPVEVQR
GLAGDPEDEHSRYLEADVFGLRVVCIYLPNGNPQPGPKFDYKLRWMERLRARMADIASEEVPALVIGDYNVIPEDKDTFS
VKAMASDALMQPESRDAYRRLLNDGWTDAIDLFNPQGGVWTFWDYQAGAWQRDHGFRIDHALLSPELADRLVAAGVDKDY
RGREKASDHAPVWVTLRD

Specific function: Major apurinic-apyrimidinic endonuclease of E.coli. It removes the damaged DNA at cytosines and guanines by cleaving on the 3'-side of the AP site by a beta-elimination reaction. It exhibits 3'-5'-exonuclease, 3'-phosphomonoesterase, 3'-repair diesterase

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=263, Percent_Identity=29.6577946768061, Blast_Score=112, Evalue=4e-25,
Organism=Homo sapiens, GI18375503, Length=263, Percent_Identity=29.6577946768061, Blast_Score=112, Evalue=4e-25,
Organism=Homo sapiens, GI18375501, Length=263, Percent_Identity=29.6577946768061, Blast_Score=112, Evalue=4e-25,
Organism=Escherichia coli, GI1788046, Length=268, Percent_Identity=36.1940298507463, Blast_Score=155, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI71989536, Length=257, Percent_Identity=26.0700389105058, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI221330655, Length=261, Percent_Identity=28.735632183908, Blast_Score=99, Evalue=4e-21,
Organism=Drosophila melanogaster, GI17136678, Length=261, Percent_Identity=28.735632183908, Blast_Score=98, Evalue=5e-21,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 29373; Mature: 29242

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLKVASFNINGIKARLPRLLEWLEETRPSVACLQEIKTQDEGFPAEEFEKIGYRAIWHG
CEEEEEEECCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHCCCEEEECC
QKGFNGVAILADGEQPVEVQRGLAGDPEDEHSRYLEADVFGLRVVCIYLPNGNPQPGPKF
CCCCCEEEEEECCCCCHHHHCCCCCCCCHHHHHHHHHHEEEEEEEEEEECCCCCCCCCCC
DYKLRWMERLRARMADIASEEVPALVIGDYNVIPEDKDTFSVKAMASDALMQPESRDAYR
CHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCEEEEEHHHHHHCCCCCHHHHH
RLLNDGWTDAIDLFNPQGGVWTFWDYQAGAWQRDHGFRIDHALLSPELADRLVAAGVDKD
HHHHCCCCCHHHCCCCCCCEEEEEECCCCCEECCCCCCHHHHHCCHHHHHHHHHHCCCCC
YRGREKASDHAPVWVTLRD
CCCCCCCCCCCCEEEEECC
>Mature Secondary Structure 
TLKVASFNINGIKARLPRLLEWLEETRPSVACLQEIKTQDEGFPAEEFEKIGYRAIWHG
EEEEEEECCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHCCCEEEECC
QKGFNGVAILADGEQPVEVQRGLAGDPEDEHSRYLEADVFGLRVVCIYLPNGNPQPGPKF
CCCCCEEEEEECCCCCHHHHCCCCCCCCHHHHHHHHHHEEEEEEEEEEECCCCCCCCCCC
DYKLRWMERLRARMADIASEEVPALVIGDYNVIPEDKDTFSVKAMASDALMQPESRDAYR
CHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCEEEEEHHHHHHCCCCCHHHHH
RLLNDGWTDAIDLFNPQGGVWTFWDYQAGAWQRDHGFRIDHALLSPELADRLVAAGVDKD
HHHHCCCCCHHHCCCCCCCEEEEEECCCCCEECCCCCCHHHHHCCHHHHHHHHHHCCCCC
YRGREKASDHAPVWVTLRD
CCCCCCCCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3049539; 9097039; 9278503; 8948651; 7885481 [H]