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The map label for this gene is gsk [C]

Identifier: 87198177

GI number: 87198177

Start: 150718

End: 151713

Strand: Direct

Name: gsk [C]

Synonym: Saro_0151

Alternate gene names: 87198177

Gene position: 150718-151713 (Clockwise)

Preceding gene: 87198175

Following gene: 87198180

Centisome position: 4.23

GC content: 66.87

Gene sequence:

>996_bases
ATGTCCGACCCGCTTTACGACGTCATCGCCATCGGTAACGCCATCGTCGACGTGATGGCCCCGTGCGAGGATGCCGACAT
CGCGCGGCTCGGTCTGGCCAAGGGCGGGATGACGCTGGTCGACACGGCTCGGGCCAAGGAACTTTACGACGCCATGGGCC
CTGCCCGCGAGATTTCGGGCGGATCGGCGGCGAACACGCTGGCCGGGCTCGCCGCGCTCGGCGCGAACTGCGCCTTCATC
GGACAAGTGGCGGACGATCAGCTCGGCGAAGTCTTTGCGCACGACATCCGCGCGGGAGGAATCGCCTTTGACACGCCGAC
CCGCGCGGACGAACCGCCGACGGCGCGGTGCCTTATCTTCGTGACGCCCGACGGGCAGCGCACGATGAACACCTTCCTCG
GGGCGTCGCAGTTCCTCCCGGCGGAGGCTCTTGACGACGCCACCATCGCGGCCGCGCAGGTGCTCTACCTCGAAGGCTAC
CTGTGGGATCCGGAAGAGCCGCGCAAGGCGATGCGCCGCGCGATCGCGGCAGCCCGCAACGCCGGTCGCAAGGTCGCCTT
CACCTTGTCCGACGCATTCGTCATCTCGCGTCATGGCGACGATTTCCGCGCGCTCATCGATGCCGGCCAGATCGACATCC
TTTTCGCCAACGAGCATGAACTGGCTGCGCTGACCGGCATCGAGGATTTCCACGTCGGGATCGAGCAACTGGCGGCCAAG
GTGCCCACCGTCGTGGTAACCCGCAGCGAGAATGGTGCCCACGCGATCAGCCAGGGCGAACGCGCTCATGTCCCGGCGGA
ACCCATCGCCAAGGTCGTCGACACCACCGGCGCCGGCGACCTTTTTGCCGCCGGCTTCCTCTATGGCTACGTTCGCGGCC
GTCCTCTCGCCGAAAGCCTTACCCTCGGTGCGATCTGCGCCGCGGAAATCATCTCGCACTACGGCGCAAGGCCCGAGGCG
GACCTCAAGGACCTGGTCGCACAGCGCTTGGGTTGA

Upstream 100 bases:

>100_bases
GCTCAATCCGACGCGTGACCGTGGCGTTCCGGCGCTTGCTGCGATGCGGGCGAGCGGCTAGGCCCTGCCCTTCCCTGTGC
CAAATGGAGCTTTTCCGCGC

Downstream 100 bases:

>100_bases
ACCTTTCCTCGTCACCGAACCATGAACGCTTCGATCTCCTGCACTGTGCGCGGGATGCCGGTACTCAGGCGTTCGCAGCC
GTTGGCGGTCACGAGGTAAA

Product: PfkB

Products: ADP; H+; IMP [C]

Alternate protein names: NA

Number of amino acids: Translated: 331; Mature: 330

Protein sequence:

>331_residues
MSDPLYDVIAIGNAIVDVMAPCEDADIARLGLAKGGMTLVDTARAKELYDAMGPAREISGGSAANTLAGLAALGANCAFI
GQVADDQLGEVFAHDIRAGGIAFDTPTRADEPPTARCLIFVTPDGQRTMNTFLGASQFLPAEALDDATIAAAQVLYLEGY
LWDPEEPRKAMRRAIAAARNAGRKVAFTLSDAFVISRHGDDFRALIDAGQIDILFANEHELAALTGIEDFHVGIEQLAAK
VPTVVVTRSENGAHAISQGERAHVPAEPIAKVVDTTGAGDLFAAGFLYGYVRGRPLAESLTLGAICAAEIISHYGARPEA
DLKDLVAQRLG

Sequences:

>Translated_331_residues
MSDPLYDVIAIGNAIVDVMAPCEDADIARLGLAKGGMTLVDTARAKELYDAMGPAREISGGSAANTLAGLAALGANCAFI
GQVADDQLGEVFAHDIRAGGIAFDTPTRADEPPTARCLIFVTPDGQRTMNTFLGASQFLPAEALDDATIAAAQVLYLEGY
LWDPEEPRKAMRRAIAAARNAGRKVAFTLSDAFVISRHGDDFRALIDAGQIDILFANEHELAALTGIEDFHVGIEQLAAK
VPTVVVTRSENGAHAISQGERAHVPAEPIAKVVDTTGAGDLFAAGFLYGYVRGRPLAESLTLGAICAAEIISHYGARPEA
DLKDLVAQRLG
>Mature_330_residues
SDPLYDVIAIGNAIVDVMAPCEDADIARLGLAKGGMTLVDTARAKELYDAMGPAREISGGSAANTLAGLAALGANCAFIG
QVADDQLGEVFAHDIRAGGIAFDTPTRADEPPTARCLIFVTPDGQRTMNTFLGASQFLPAEALDDATIAAAQVLYLEGYL
WDPEEPRKAMRRAIAAARNAGRKVAFTLSDAFVISRHGDDFRALIDAGQIDILFANEHELAALTGIEDFHVGIEQLAAKV
PTVVVTRSENGAHAISQGERAHVPAEPIAKVVDTTGAGDLFAAGFLYGYVRGRPLAESLTLGAICAAEIISHYGARPEAD
LKDLVAQRLG

Specific function: Unknown

COG id: COG0524

COG function: function code G; Sugar kinases, ribokinase family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the carbohydrate kinase pfkB family [H]

Homologues:

Organism=Homo sapiens, GI5670342, Length=280, Percent_Identity=24.2857142857143, Blast_Score=69, Evalue=4e-12,
Organism=Escherichia coli, GI1786684, Length=242, Percent_Identity=28.9256198347107, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI87082343, Length=271, Percent_Identity=28.0442804428044, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI87081971, Length=331, Percent_Identity=26.8882175226586, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1790193, Length=274, Percent_Identity=28.4671532846715, Blast_Score=65, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI17541820, Length=337, Percent_Identity=23.1454005934718, Blast_Score=75, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI17532905, Length=281, Percent_Identity=27.7580071174377, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI21355521, Length=341, Percent_Identity=25.5131964809384, Blast_Score=89, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011611
- InterPro:   IPR002173 [H]

Pfam domain/function: PF00294 PfkB [H]

EC number: 2.7.1.73 [C]

Molecular weight: Translated: 34785; Mature: 34654

Theoretical pI: Translated: 4.48; Mature: 4.48

Prosite motif: PS00584 PFKB_KINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDPLYDVIAIGNAIVDVMAPCEDADIARLGLAKGGMTLVDTARAKELYDAMGPAREISG
CCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCEEEHHHHHHHHHHHCCCHHHCCC
GSAANTLAGLAALGANCAFIGQVADDQLGEVFAHDIRAGGIAFDTPTRADEPPTARCLIF
CCHHHHHHHHHHHCCCCEEEECHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCEEEEE
VTPDGQRTMNTFLGASQFLPAEALDDATIAAAQVLYLEGYLWDPEEPRKAMRRAIAAARN
ECCCCCHHHHHHHCHHHCCCHHHCCCHHHHHHHHHHEECEECCCCHHHHHHHHHHHHHHC
AGRKVAFTLSDAFVISRHGDDFRALIDAGQIDILFANEHELAALTGIEDFHVGIEQLAAK
CCCEEEEEEECEEEEECCCCHHHHHHCCCCEEEEEECCCCEEEECCCHHHHHHHHHHHHH
VPTVVVTRSENGAHAISQGERAHVPAEPIAKVVDTTGAGDLFAAGFLYGYVRGRPLAESL
CCEEEEEECCCCCHHHCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHHH
TLGAICAAEIISHYGARPEADLKDLVAQRLG
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHCC
>Mature Secondary Structure 
SDPLYDVIAIGNAIVDVMAPCEDADIARLGLAKGGMTLVDTARAKELYDAMGPAREISG
CCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCEEEHHHHHHHHHHHCCCHHHCCC
GSAANTLAGLAALGANCAFIGQVADDQLGEVFAHDIRAGGIAFDTPTRADEPPTARCLIF
CCHHHHHHHHHHHCCCCEEEECHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCEEEEE
VTPDGQRTMNTFLGASQFLPAEALDDATIAAAQVLYLEGYLWDPEEPRKAMRRAIAAARN
ECCCCCHHHHHHHCHHHCCCHHHCCCHHHHHHHHHHEECEECCCCHHHHHHHHHHHHHHC
AGRKVAFTLSDAFVISRHGDDFRALIDAGQIDILFANEHELAALTGIEDFHVGIEQLAAK
CCCEEEEEEECEEEEECCCCHHHHHHCCCCEEEEEECCCCEEEECCCHHHHHHHHHHHHH
VPTVVVTRSENGAHAISQGERAHVPAEPIAKVVDTTGAGDLFAAGFLYGYVRGRPLAESL
CCEEEEEECCCCCHHHCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHHH
TLGAICAAEIISHYGARPEADLKDLVAQRLG
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; Inosine [C]

Specific reaction: ATP + Inosine --> ADP + H+ + IMP [C]

General reaction: Phospho group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]