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The map label for this gene is nth [H]

Identifier: 87198032

GI number: 87198032

Start: 5121

End: 5816

Strand: Direct

Name: nth [H]

Synonym: Saro_0006

Alternate gene names: 87198032

Gene position: 5121-5816 (Clockwise)

Preceding gene: 87198031

Following gene: 87198033

Centisome position: 0.14

GC content: 63.22

Gene sequence:

>696_bases
ATGACGCGCGACCAGATATTCGAGTTCTTCCGCCGACTTGCCGAGGCAAACCCCTCGCCCGAGACCGAGCTGGAGTTCGG
CAACGTCTACCAGTTGCTGGTCGCGGTGACGCTTTCGGCGCAGGCGACCGACGTCGGCGTCAACAAGGCCACCCGCAAGC
TGTTCCAGATCGTCAAGACGCCGCAGGACATGCTGGATCTTGGCGAGGAAGGCCTGAAGGAACACATCAAGACCATCGGC
CTGTTCAATTCCAAGGCCCGGAACGTCATGGCCATGGCCGAAATTCTCGTGCGCGAGCATGGGGGCGAAGTGCCGGCCGA
CCGCGACCTGCTGACCGCGTTGCCAGGCGTGGGCCGCAAGACCGCCAACGTCGTGCTGAATTGTGCGTTCGGCGCGGAAA
CATTTGCGGTCGACACACATATCTTCCGCGTGGGCAACCGCACCGGCCTTGCGAAGGGCAAGACGCCGCTGGCGGTGGAG
AAGCAGCTCGAGCGCAAGGTCCCCGGTCCGTTCAGGGTCGGTGCGCACCACTGGCTGATCTTGCACGGCCGCTATGTCTG
CAAGGCGCGTACGCCCGAATGCTGGCATTGCGGGGTGGTTGACCTTTGCGGCTACAAGGCGAAGGTTCTGGAAAAGGGGG
CAATGCCGGCTGGCGCGAAGAAGGCGGTGGCCGTCAAGAAGGAGAGCAAGGCATGA

Upstream 100 bases:

>100_bases
AGGAAGCGATCTTCTATACCCACCCTTCGGTCGAACGTCGCATCGAGGCGGCGATGGCCTGGAAGGCAGGGCGTGGCGGT
GCGAACATGACCGGGGTCCA

Downstream 100 bases:

>100_bases
AGCGCATGACTCCGGTCCTTTTCGCCGCCGCATTGGCGCTTTCCGGCTGTGCCGCCCAGGGCGGATCGGGCAACGACGGT
CACGGCAAGGACGTTCCCGC

Product: DNA-(apurinic or apyrimidinic site) lyase / endonuclease III

Products: NA

Alternate protein names: DNA-(apurinic or apyrimidinic site) lyase [H]

Number of amino acids: Translated: 231; Mature: 230

Protein sequence:

>231_residues
MTRDQIFEFFRRLAEANPSPETELEFGNVYQLLVAVTLSAQATDVGVNKATRKLFQIVKTPQDMLDLGEEGLKEHIKTIG
LFNSKARNVMAMAEILVREHGGEVPADRDLLTALPGVGRKTANVVLNCAFGAETFAVDTHIFRVGNRTGLAKGKTPLAVE
KQLERKVPGPFRVGAHHWLILHGRYVCKARTPECWHCGVVDLCGYKAKVLEKGAMPAGAKKAVAVKKESKA

Sequences:

>Translated_231_residues
MTRDQIFEFFRRLAEANPSPETELEFGNVYQLLVAVTLSAQATDVGVNKATRKLFQIVKTPQDMLDLGEEGLKEHIKTIG
LFNSKARNVMAMAEILVREHGGEVPADRDLLTALPGVGRKTANVVLNCAFGAETFAVDTHIFRVGNRTGLAKGKTPLAVE
KQLERKVPGPFRVGAHHWLILHGRYVCKARTPECWHCGVVDLCGYKAKVLEKGAMPAGAKKAVAVKKESKA
>Mature_230_residues
TRDQIFEFFRRLAEANPSPETELEFGNVYQLLVAVTLSAQATDVGVNKATRKLFQIVKTPQDMLDLGEEGLKEHIKTIGL
FNSKARNVMAMAEILVREHGGEVPADRDLLTALPGVGRKTANVVLNCAFGAETFAVDTHIFRVGNRTGLAKGKTPLAVEK
QLERKVPGPFRVGAHHWLILHGRYVCKARTPECWHCGVVDLCGYKAKVLEKGAMPAGAKKAVAVKKESKA

Specific function: Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand, 5' from the damaged site [H]

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI4505471, Length=177, Percent_Identity=31.0734463276836, Blast_Score=80, Evalue=1e-15,
Organism=Escherichia coli, GI1787920, Length=209, Percent_Identity=59.8086124401914, Blast_Score=253, Evalue=5e-69,
Organism=Caenorhabditis elegans, GI17554540, Length=169, Percent_Identity=33.7278106508876, Blast_Score=90, Evalue=8e-19,
Organism=Drosophila melanogaster, GI45550361, Length=177, Percent_Identity=28.8135593220339, Blast_Score=82, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR005759
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170 [H]

Pfam domain/function: PF10576 EndIII_4Fe-2S; PF00633 HHH; PF00730 HhH-GPD [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 25317; Mature: 25186

Theoretical pI: Translated: 9.87; Mature: 9.87

Prosite motif: PS01155 ENDONUCLEASE_III_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRDQIFEFFRRLAEANPSPETELEFGNVYQLLVAVTLSAQATDVGVNKATRKLFQIVKT
CCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHC
PQDMLDLGEEGLKEHIKTIGLFNSKARNVMAMAEILVREHGGEVPADRDLLTALPGVGRK
CHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCCC
TANVVLNCAFGAETFAVDTHIFRVGNRTGLAKGKTPLAVEKQLERKVPGPFRVGAHHWLI
CCCEEEEEECCCCCEEEHHHEEEECCCCCCCCCCCCHHHHHHHHHCCCCCEEECCEEEEE
LHGRYVCKARTPECWHCGVVDLCGYKAKVLEKGAMPAGAKKAVAVKKESKA
EECEEEEECCCCCCCCCCHHHHHCCHHHHHHCCCCCCCCCHHEEECCCCCC
>Mature Secondary Structure 
TRDQIFEFFRRLAEANPSPETELEFGNVYQLLVAVTLSAQATDVGVNKATRKLFQIVKT
CHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHC
PQDMLDLGEEGLKEHIKTIGLFNSKARNVMAMAEILVREHGGEVPADRDLLTALPGVGRK
CHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCCC
TANVVLNCAFGAETFAVDTHIFRVGNRTGLAKGKTPLAVEKQLERKVPGPFRVGAHHWLI
CCCEEEEEECCCCCEEEHHHEEEECCCCCCCCCCCCHHHHHHHHHCCCCCEEECCEEEEE
LHGRYVCKARTPECWHCGVVDLCGYKAKVLEKGAMPAGAKKAVAVKKESKA
EECEEEEECCCCCCCCCCHHHHHCCHHHHHHCCCCCCCCCHHEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]