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The map label for this gene is caiC [H]

Identifier: 86751803

GI number: 86751803

Start: 5324776

End: 5326362

Strand: Direct

Name: caiC [H]

Synonym: RPB_4705

Alternate gene names: 86751803

Gene position: 5324776-5326362 (Clockwise)

Preceding gene: 86751801

Following gene: 86751805

Centisome position: 99.87

GC content: 69.57

Gene sequence:

>1587_bases
ATGCCGCAACCCAGAGAAGCGCCGGGGATTGTCAGTCCGTTCGCCGGCATGGACGTGCCATGGCTGCTGCGGCTGCACGC
GCAGGCGCGGCCCGAACATCCCTTCGTGATCTGGGCGCCGTTCGACGCCCCGGCGCGGGTCTGGCGCTACGGCGAATTCC
ACGACCGCGTCGGCGCGCTCGCCGCCGGCCTGGTCGCGCGCGGCGTCCGGCCCGGCGACGCGGTGCTGATCCATCTCGAC
AATTGCGCCGAATTCCTGCTCGCCTGGTTCGCCTGCGTCGAGCTCGGCGCGCTCGCGGTGACCACCAACACCCGCTCGGC
GGCGGCCGAGATCGATTACTTCGCCGACCACTGCAACGCCGTCGCCGCCATCACCCAGCCGGCCTATGCCGAACTGGTCG
CGGCGAACTGCAAGAAGCTGCGCTGGATCGCGGTGATCTCGCACGATCCCGGCTCGCCGCTGCCGGCGCAGCCGGTGGCG
AAGGCGGAGTCGTTCGACAGCCTGTTCGGCGACAGCGCCAACCGGCCGCGGCGGCCGACCGACCCGTTCGCGCCGTGCAG
CGTGCAGTACACTTCGGGCACCACGTCGCGGCCGAAGGCGGTGCTGTGGACCCACGCCAACGCGCTGTGGGGCGCCAAGA
TCAACGCCGCCCACCAGACGCTGCGCGCCGACGACGTCCACCTCGCTTACCTGCCGCTGTTCCACACCAACGCGCTGGCC
TATTCGATGCTGGCGAGCCTGTGGGTCGGCGGCACCTGCGTGGTGCAGCCGCGGTTCTCGTCGAGCCGGTTCTGGAAGGT
GGCGCTGGAGCACCGCTGCACCTGGAATTCCAACATCGCGTTCTGCCTGAAGGCGCTGATGGATCAGGAGACGCCGAAGG
ATCACTCGTTCCGTCTGTGGGGCACCGCGGTCGCCGAACCGCCGGTCTTCGCCGGGTTCGGCATCAAGATCATCGGCTGG
TGGGGCATGACCGAGACCATCACCCACGGCATCATCGCCGAGGCCGACCAGCCCAACACGCCGATGTCGATCGGCCGCGC
CGCGCCCGAATATGCGATCCGCATCGTCGGCGAGGACGGCGCGCCGACGGCCGTCGGCGACACCGGCAATCTGCTGATCA
AGGGCGTGCCGGGCCTGTCGCTGTTCGCGGAGTATCTGCACAACGAGCAGGCCACCCGCGACAGCTTCGACGAGCACGGC
TACTTCATCACCGGCGACCGCGTCACGCTGCTGGAGCACGGCGCGATCCGGTTCGGCGACCGCGCCAAGGACATGCTCAA
GGTCGGCGGCGAGAACGTAGCGGCGTCCGAGATCGAGCAGGTGGTGATCACCGTGCCGGGCGTGCGCGAGGCCGCGGTGG
TGGCGAAGAAGCACCCGATGCTCGACGAGGTGCCGGTGGTGTTCGTGATCCCGGCGGCCGGCGTCGACAAGGCGGCGCCC
GACCTCGCCGATCAGATCCTGGCGGCGTGCAGCCGGTCGCTCGCCGATTTCAAGCGCCCGCGCGAAGTGCGCCTGGTCGA
CGACATGCCGCGCTCGACGCTGGAGAAGGTGGCGAAGGCGGAATTGCGCAAGATGCTGGCGGAGTAG

Upstream 100 bases:

>100_bases
GCGGGACGGAAACAAAAGGGATGCGCGCCATGCAGCGGCAATTGCCGTCGCACGGAAAGCGCACGAACGCGCGGAAAGCG
CAGCACAAAGGGGAGAAACC

Downstream 100 bases:

>100_bases
TCGCGGCCTTCTCGTCATTCCGGGACCCGCGCAACGCGCGTGAACCCGGAATGACCGGGGAAACTAACCGCCCATCGCCA
CCGGGCGGAACGCCTGCAGC

Product: AMP-dependent synthetase and ligase

Products: D-carnitinyl-CoA; crotonobetainyl-CoA; L-carnitinyl-CoA [C]

Alternate protein names: NA

Number of amino acids: Translated: 528; Mature: 527

Protein sequence:

>528_residues
MPQPREAPGIVSPFAGMDVPWLLRLHAQARPEHPFVIWAPFDAPARVWRYGEFHDRVGALAAGLVARGVRPGDAVLIHLD
NCAEFLLAWFACVELGALAVTTNTRSAAAEIDYFADHCNAVAAITQPAYAELVAANCKKLRWIAVISHDPGSPLPAQPVA
KAESFDSLFGDSANRPRRPTDPFAPCSVQYTSGTTSRPKAVLWTHANALWGAKINAAHQTLRADDVHLAYLPLFHTNALA
YSMLASLWVGGTCVVQPRFSSSRFWKVALEHRCTWNSNIAFCLKALMDQETPKDHSFRLWGTAVAEPPVFAGFGIKIIGW
WGMTETITHGIIAEADQPNTPMSIGRAAPEYAIRIVGEDGAPTAVGDTGNLLIKGVPGLSLFAEYLHNEQATRDSFDEHG
YFITGDRVTLLEHGAIRFGDRAKDMLKVGGENVAASEIEQVVITVPGVREAAVVAKKHPMLDEVPVVFVIPAAGVDKAAP
DLADQILAACSRSLADFKRPREVRLVDDMPRSTLEKVAKAELRKMLAE

Sequences:

>Translated_528_residues
MPQPREAPGIVSPFAGMDVPWLLRLHAQARPEHPFVIWAPFDAPARVWRYGEFHDRVGALAAGLVARGVRPGDAVLIHLD
NCAEFLLAWFACVELGALAVTTNTRSAAAEIDYFADHCNAVAAITQPAYAELVAANCKKLRWIAVISHDPGSPLPAQPVA
KAESFDSLFGDSANRPRRPTDPFAPCSVQYTSGTTSRPKAVLWTHANALWGAKINAAHQTLRADDVHLAYLPLFHTNALA
YSMLASLWVGGTCVVQPRFSSSRFWKVALEHRCTWNSNIAFCLKALMDQETPKDHSFRLWGTAVAEPPVFAGFGIKIIGW
WGMTETITHGIIAEADQPNTPMSIGRAAPEYAIRIVGEDGAPTAVGDTGNLLIKGVPGLSLFAEYLHNEQATRDSFDEHG
YFITGDRVTLLEHGAIRFGDRAKDMLKVGGENVAASEIEQVVITVPGVREAAVVAKKHPMLDEVPVVFVIPAAGVDKAAP
DLADQILAACSRSLADFKRPREVRLVDDMPRSTLEKVAKAELRKMLAE
>Mature_527_residues
PQPREAPGIVSPFAGMDVPWLLRLHAQARPEHPFVIWAPFDAPARVWRYGEFHDRVGALAAGLVARGVRPGDAVLIHLDN
CAEFLLAWFACVELGALAVTTNTRSAAAEIDYFADHCNAVAAITQPAYAELVAANCKKLRWIAVISHDPGSPLPAQPVAK
AESFDSLFGDSANRPRRPTDPFAPCSVQYTSGTTSRPKAVLWTHANALWGAKINAAHQTLRADDVHLAYLPLFHTNALAY
SMLASLWVGGTCVVQPRFSSSRFWKVALEHRCTWNSNIAFCLKALMDQETPKDHSFRLWGTAVAEPPVFAGFGIKIIGWW
GMTETITHGIIAEADQPNTPMSIGRAAPEYAIRIVGEDGAPTAVGDTGNLLIKGVPGLSLFAEYLHNEQATRDSFDEHGY
FITGDRVTLLEHGAIRFGDRAKDMLKVGGENVAASEIEQVVITVPGVREAAVVAKKHPMLDEVPVVFVIPAAGVDKAAPD
LADQILAACSRSLADFKRPREVRLVDDMPRSTLEKVAKAELRKMLAE

Specific function: Could catalyze the transfer of CoA to carnitine, generating the initial carnitinyl-CoA needed for the CaiB reaction cycle [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI40807357, Length=543, Percent_Identity=24.3093922651934, Blast_Score=117, Evalue=3e-26,
Organism=Homo sapiens, GI38505220, Length=532, Percent_Identity=24.4360902255639, Blast_Score=114, Evalue=3e-25,
Organism=Homo sapiens, GI187761345, Length=496, Percent_Identity=26.008064516129, Blast_Score=112, Evalue=6e-25,
Organism=Homo sapiens, GI187761343, Length=496, Percent_Identity=26.008064516129, Blast_Score=112, Evalue=6e-25,
Organism=Homo sapiens, GI42544132, Length=536, Percent_Identity=23.8805970149254, Blast_Score=112, Evalue=8e-25,
Organism=Homo sapiens, GI156151445, Length=517, Percent_Identity=22.6305609284333, Blast_Score=108, Evalue=2e-23,
Organism=Homo sapiens, GI13236579, Length=488, Percent_Identity=24.3852459016393, Blast_Score=103, Evalue=5e-22,
Organism=Homo sapiens, GI38524616, Length=548, Percent_Identity=25.3649635036496, Blast_Score=101, Evalue=2e-21,
Organism=Homo sapiens, GI122937307, Length=526, Percent_Identity=23.1939163498099, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI157311624, Length=520, Percent_Identity=22.5, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI157311622, Length=520, Percent_Identity=22.5, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI115511026, Length=548, Percent_Identity=23.3576642335766, Blast_Score=100, Evalue=5e-21,
Organism=Homo sapiens, GI58082049, Length=520, Percent_Identity=22.3076923076923, Blast_Score=99, Evalue=9e-21,
Organism=Homo sapiens, GI227499619, Length=536, Percent_Identity=24.2537313432836, Blast_Score=94, Evalue=5e-19,
Organism=Homo sapiens, GI13375727, Length=552, Percent_Identity=20.2898550724638, Blast_Score=87, Evalue=5e-17,
Organism=Homo sapiens, GI13325057, Length=369, Percent_Identity=26.2872628726287, Blast_Score=87, Evalue=5e-17,
Organism=Homo sapiens, GI8923896, Length=538, Percent_Identity=23.0483271375465, Blast_Score=67, Evalue=4e-11,
Organism=Escherichia coli, GI221142682, Length=494, Percent_Identity=30.5668016194332, Blast_Score=211, Evalue=6e-56,
Organism=Escherichia coli, GI145693145, Length=517, Percent_Identity=23.9845261121857, Blast_Score=132, Evalue=6e-32,
Organism=Escherichia coli, GI1788107, Length=516, Percent_Identity=26.1627906976744, Blast_Score=123, Evalue=3e-29,
Organism=Escherichia coli, GI1786810, Length=508, Percent_Identity=23.8188976377953, Blast_Score=118, Evalue=1e-27,
Organism=Escherichia coli, GI1790505, Length=548, Percent_Identity=23.1751824817518, Blast_Score=82, Evalue=8e-17,
Organism=Escherichia coli, GI1786529, Length=545, Percent_Identity=24.0366972477064, Blast_Score=69, Evalue=9e-13,
Organism=Caenorhabditis elegans, GI17558820, Length=508, Percent_Identity=26.9685039370079, Blast_Score=152, Evalue=5e-37,
Organism=Caenorhabditis elegans, GI32563687, Length=497, Percent_Identity=28.3702213279678, Blast_Score=152, Evalue=6e-37,
Organism=Caenorhabditis elegans, GI115534440, Length=523, Percent_Identity=23.5181644359465, Blast_Score=117, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17531443, Length=504, Percent_Identity=24.2063492063492, Blast_Score=113, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17557194, Length=551, Percent_Identity=23.5934664246824, Blast_Score=110, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17538037, Length=563, Percent_Identity=22.9129662522202, Blast_Score=107, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17560308, Length=562, Percent_Identity=23.8434163701068, Blast_Score=106, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI71994694, Length=546, Percent_Identity=24.1758241758242, Blast_Score=105, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI71994703, Length=546, Percent_Identity=24.1758241758242, Blast_Score=105, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI71994690, Length=546, Percent_Identity=24.1758241758242, Blast_Score=105, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI17551278, Length=530, Percent_Identity=24.3396226415094, Blast_Score=101, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17560140, Length=359, Percent_Identity=24.7910863509749, Blast_Score=91, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI71985884, Length=143, Percent_Identity=33.5664335664336, Blast_Score=68, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6319699, Length=492, Percent_Identity=22.7642276422764, Blast_Score=98, Evalue=4e-21,
Organism=Saccharomyces cerevisiae, GI6319264, Length=557, Percent_Identity=23.3393177737882, Blast_Score=79, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24583465, Length=537, Percent_Identity=24.7672253258845, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI24583463, Length=537, Percent_Identity=24.7672253258845, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI281364798, Length=537, Percent_Identity=24.7672253258845, Blast_Score=114, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24581924, Length=525, Percent_Identity=25.5238095238095, Blast_Score=112, Evalue=9e-25,
Organism=Drosophila melanogaster, GI45552801, Length=528, Percent_Identity=23.8636363636364, Blast_Score=105, Evalue=6e-23,
Organism=Drosophila melanogaster, GI45550496, Length=528, Percent_Identity=23.8636363636364, Blast_Score=105, Evalue=6e-23,
Organism=Drosophila melanogaster, GI21355181, Length=502, Percent_Identity=22.7091633466135, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI18859661, Length=510, Percent_Identity=24.7058823529412, Blast_Score=82, Evalue=6e-16,
Organism=Drosophila melanogaster, GI62472339, Length=555, Percent_Identity=22.8828828828829, Blast_Score=79, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24667955, Length=535, Percent_Identity=23.3644859813084, Blast_Score=78, Evalue=2e-14,
Organism=Drosophila melanogaster, GI20130357, Length=209, Percent_Identity=28.2296650717703, Blast_Score=73, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24667959, Length=365, Percent_Identity=24.1095890410959, Blast_Score=73, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24648257, Length=278, Percent_Identity=26.978417266187, Blast_Score=69, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.3.2.- [C]

Molecular weight: Translated: 57424; Mature: 57293

Theoretical pI: Translated: 6.65; Mature: 6.65

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPQPREAPGIVSPFAGMDVPWLLRLHAQARPEHPFVIWAPFDAPARVWRYGEFHDRVGAL
CCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCEEEEECCCCCHHHHCCCCHHHHHHHH
AAGLVARGVRPGDAVLIHLDNCAEFLLAWFACVELGALAVTTNTRSAAAEIDYFADHCNA
HHHHHHCCCCCCCEEEEEEHHHHHHHHHHHHHHHHCEEEEEECCCHHHHHHHHHHHHCCC
VAAITQPAYAELVAANCKKLRWIAVISHDPGSPLPAQPVAKAESFDSLFGDSANRPRRPT
EEHHCCHHHHHHHHHCCCEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCC
DPFAPCSVQYTSGTTSRPKAVLWTHANALWGAKINAAHQTLRADDVHLAYLPLFHTNALA
CCCCCCEEEECCCCCCCCCEEEEEECCEECCCEEHHHHHHHCCCCEEEEEEEHHHHHHHH
YSMLASLWVGGTCVVQPRFSSSRFWKVALEHRCTWNSNIAFCLKALMDQETPKDHSFRLW
HHHHHHHHCCCEEEEECCCCCCCEEEEEEHHCCCCCCCHHHHHHHHHCCCCCCCCCEEEE
GTAVAEPPVFAGFGIKIIGWWGMTETITHGIIAEADQPNTPMSIGRAAPEYAIRIVGEDG
EEECCCCCEEECCCEEEEEECCCHHHHHHCCEECCCCCCCCHHHCCCCCCEEEEEEECCC
APTAVGDTGNLLIKGVPGLSLFAEYLHNEQATRDSFDEHGYFITGDRVTLLEHGAIRFGD
CCCCCCCCCCEEEECCCCHHHHHHHHCCCHHHHCCHHHCCEEEECCEEEEEECCCEECCH
RAKDMLKVGGENVAASEIEQVVITVPGVREAAVVAKKHPMLDEVPVVFVIPAAGVDKAAP
HHHHHHHHCCCCCCHHHHCEEEEECCCCHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCH
DLADQILAACSRSLADFKRPREVRLVDDMPRSTLEKVAKAELRKMLAE
HHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PQPREAPGIVSPFAGMDVPWLLRLHAQARPEHPFVIWAPFDAPARVWRYGEFHDRVGAL
CCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCEEEEECCCCCHHHHCCCCHHHHHHHH
AAGLVARGVRPGDAVLIHLDNCAEFLLAWFACVELGALAVTTNTRSAAAEIDYFADHCNA
HHHHHHCCCCCCCEEEEEEHHHHHHHHHHHHHHHHCEEEEEECCCHHHHHHHHHHHHCCC
VAAITQPAYAELVAANCKKLRWIAVISHDPGSPLPAQPVAKAESFDSLFGDSANRPRRPT
EEHHCCHHHHHHHHHCCCEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCC
DPFAPCSVQYTSGTTSRPKAVLWTHANALWGAKINAAHQTLRADDVHLAYLPLFHTNALA
CCCCCCEEEECCCCCCCCCEEEEEECCEECCCEEHHHHHHHCCCCEEEEEEEHHHHHHHH
YSMLASLWVGGTCVVQPRFSSSRFWKVALEHRCTWNSNIAFCLKALMDQETPKDHSFRLW
HHHHHHHHCCCEEEEECCCCCCCEEEEEEHHCCCCCCCHHHHHHHHHCCCCCCCCCEEEE
GTAVAEPPVFAGFGIKIIGWWGMTETITHGIIAEADQPNTPMSIGRAAPEYAIRIVGEDG
EEECCCCCEEECCCEEEEEECCCHHHHHHCCEECCCCCCCCHHHCCCCCCEEEEEEECCC
APTAVGDTGNLLIKGVPGLSLFAEYLHNEQATRDSFDEHGYFITGDRVTLLEHGAIRFGD
CCCCCCCCCCEEEECCCCHHHHHHHHCCCHHHHCCHHHCCEEEECCEEEEEECCCEECCH
RAKDMLKVGGENVAASEIEQVVITVPGVREAAVVAKKHPMLDEVPVVFVIPAAGVDKAAP
HHHHHHHHCCCCCCHHHHCEEEEECCCCHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCH
DLADQILAACSRSLADFKRPREVRLVDDMPRSTLEKVAKAELRKMLAE
HHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Coenzyme A; D-carnitine; Coenzyme A; crotono-betaine; L-carnitine [C]

Specific reaction: Coenzyme A + D-carnitine = D-carnitinyl-CoA Coenzyme A + crotono-betaine = crotonobetainyl-CoA Coenzyme A + L-carnitine = L-carnitinyl-CoA [C]

General reaction: Ligases; Forming Carbon-Sulfur Bonds; Acid-Thiol Ligases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA